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Method for extracting theanine from fresh tea

A technology of fresh tea and theanine, which is applied in the separation/purification of carboxylic acid amide, organic chemistry, etc., can solve the problems of low purity and achieve the effects of high purity, short production cycle and high separation efficiency

Active Publication Date: 2014-06-04
CHENGDU WAGOTT BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the present invention utilizes various tea raw materials to extract theanine, which has the advantages of simple operation, low cost, and no environmental pollution, but this method uses dry tea leaves, and the obtained theanine is only a crude product with a low purity of only 10 %-30%

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  • Method for extracting theanine from fresh tea

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: a kind of method extracting theanine from fresh tealeaves, it comprises the following steps:

[0024] S1. Soaking: put 2,000 kg of fresh tea leaves in a container, add 10,000 kg of purified water, soak at a constant temperature for 1.8 hours, and pass the mixed solution in the container through a 18-mesh sieve to obtain a filtrate; wherein, the soaking temperature at a constant temperature is 65°C;

[0025] S2. Centrifugation: Centrifuge the filtrate for 50 minutes under an environment with a temperature of 30° C. and a pressure of 0.1 MPa, discard the precipitate, and use the centrifuge for the next operation; wherein, the speed of the centrifuge is 6500 rpm;

[0026] S3. Concentration once: use a reverse osmosis membrane with a molecular weight cut-off of 100 to concentrate the centrifugate at a concentration temperature of 30°C until the solid content is 5%;

[0027] S4. Primary adsorption: load the concentrated solution on LX-8 resin, elute with 70% et...

Embodiment 2

[0035] Embodiment 2: a kind of method extracting theanine from fresh tealeaves, it comprises the following steps:

[0036] S1. Soaking: put 2000kg of fresh tea leaves in an extractor, add 16000kg of purified water, soak at a constant temperature for 2.5 hours, pass the mixed solution in the extractor through a 24-mesh sieve to obtain a filtrate; wherein, the constant temperature soaking temperature is 100°C ;

[0037] S2. Centrifugation: Centrifuge the filtrate for 70 minutes under an environment with a temperature of 50° C. and a pressure of 0.3 MPa, discard the precipitate, and use the centrifugate for the next step; wherein, the speed of the centrifuge is 7000 rpm;

[0038] S3. Primary concentration: Concentrate the centrifugate with a reverse osmosis membrane with a molecular weight cut-off of 100 at a concentration temperature of 50°C until the solid content is 10%;

[0039] S4. Primary adsorption: load the concentrated solution on LX-8 resin, elute with 75% ethanol, and...

Embodiment 3

[0047] Embodiment 3: a kind of method extracting theanine from fresh tealeaves, it comprises the following steps:

[0048] S1. Soaking: put 2000kg of fresh tea leaves in an extractor, add 12000kg of purified water, soak at a constant temperature for 2 hours, pass the mixed solution in the container through a 20-mesh sieve to obtain a filtrate; wherein, the soaking temperature at a constant temperature is 85°C;

[0049] S2. Centrifugation: Centrifuge the filtrate for 60 minutes in an environment with a temperature of 38°C and a pressure of 0.25 MPa, discard the precipitate, and use the centrifuge for the next step; wherein, the speed of the centrifuge is 6800 rpm;

[0050] S3. Primary concentration: Concentrate the centrifugate with a reverse osmosis membrane with a molecular weight cut-off of 100 at a concentration temperature of 42°C until the solid content is 10%;

[0051] S4. Primary adsorption: load the concentrated solution on LX-8 resin, elute with 72% ethanol, and the e...

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Abstract

The invention discloses a method for extracting theanine from fresh tea, belonging to the technical field of natural product chemistry. The method comprises specific steps of S1. soaking; S2. centrifuging; S3. concentrating for the first time; S4. adsorbing for the first time; S5. adsorbing for the second time; S6. adsorbing for the third time; S7. concentrating for the second time; S8. concentrating for the third time; S9. disinfecting; S10. spray-drying; S11. deironing. The method adopts fresh tea as the raw material, thus overcoming the defects in the traditional technology that smashed and sieved dry tea is easy to be burnt and difficult to filter in extraction at high temperature; purified water is used as a medium for directly separating theanine, so that less fat soluble pesticide is leached; the method adopts three times of resin adsorption for respectively adsorbing EGCG (epigallocatechin gllate), caffeine and theanine in the filtrate, and finally natural theanine with content of 30-45% can be separated. The method has advantages of simple operation, high separation efficiency, low cost, short production cycle, high purity of the product, and is applicable to large scale factory production.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for extracting theanine from fresh tea leaves. Background technique [0002] Amino acid is an important component of tea, named N-acetyl-γ-L-glutamine (N-ethyl-γ-L-glatamine). Theanine is an effective taste substance in green tea. The content of theanine directly determines the quality of tea: no matter what kind of tea, theanine accounts for more than 50% of the free amino acids in tea, and the content of theanine in dry tea is about 1%-2%. Theanine is the ethylamine inducer of glutamic acid. It is synthesized from glutamic acid and ethylamine in the roots of tea trees, transported to the leaves for storage through the trunk, and converted into catechins under sunlight. In 1950, Mr. Shuto first extracted and refined theanine from tea leaves, and determined its chemical structure. Since then, many scholars have carried out systematic rese...

Claims

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Application Information

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IPC IPC(8): C07C237/06C07C231/24
Inventor 顾峰
Owner CHENGDU WAGOTT BIO TECH
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