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Method for extracting monomeric EGCG from fresh tea

A technology of fresh tea leaves and monomers, applied in the field of extracting monomer EGCG, which can solve the problems of high cost, small amount of preparation, unsuitable for large-scale factory production, etc., and achieve the effect of low cost, short production cycle and reduced leaching

Active Publication Date: 2014-05-28
CHENGDU WAGOTT BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has high cost and small amount of preparation, and is not suitable for large-scale industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: a kind of method extracting monomer EGCG from fresh tealeaves, it comprises the following steps:

[0029] S1. Soaking: put 2000kg of fresh tea leaves in the extractor, add 10000kg of purified water, soak at a constant temperature for 1.8h, pass the mixed solution in the extractor through a 18-mesh sieve, and obtain the filtrate; wherein, the constant temperature soaking temperature is 65 ℃;

[0030] S2. Centrifugation: Centrifuge the filtrate for 50 s in an environment with a temperature of 30° C. and a pressure of 0.1 MPa, discard the precipitate, and use the centrifuge for the next step; wherein, the speed of the centrifuge is 6500 rpm;

[0031] S3. Primary adsorption: put the centrifugate obtained in step S2 on the LX-8 resin, absorb EGCG in the centrifugate, and elute with 70% ethanol at a speed of 1.8m 3 / h, collecting the eluate with a solids content of 4%;

[0032] S4. MVR concentration: put the collected eluate into the MVR concentrator to concen...

Embodiment 2

[0040] Embodiment 2: a kind of method extracting monomer EGCG from fresh tealeaves, it comprises the following steps:

[0041] S1. Soaking: put 2000kg of fresh tea leaves in the extractor, add 16000kg of purified water, soak for 2.5 hours at a constant temperature, pass the mixed solution in the extractor through a 24 mesh sieve, and obtain the filtrate; wherein, the constant temperature soaking temperature is 100 ℃;

[0042] S2. Centrifugation: Centrifuge the filtrate for 70 seconds under an environment with a temperature of 50° C. and a pressure of 0.3 MPa, discard the precipitate, and use the centrifuge for the next operation; wherein, the speed of the centrifuge is 7000 rpm;

[0043] S3. Primary adsorption: load the centrifugate obtained in step S2 on LX-8 resin, absorb EGCG in the centrifugate, and elute with 75% ethanol at a speed of 2.4m 3 / h, collecting the eluent with a solids content of 6%;

[0044] S4. MVR concentration: put the collected eluate into the MVR conce...

Embodiment 3

[0052] Embodiment 3: a kind of method extracting monomer EGCG from fresh tealeaves, it comprises the following steps:

[0053] S1. Soaking: put 2000kg of fresh tea leaves in an extractor, add 12000kg of purified water, soak at a constant temperature for 2 hours, and pass the mixed solution in the extractor through a 20-mesh sieve to obtain a filtrate; wherein, the constant temperature soaking temperature is 85°C ;

[0054] S2. Centrifugation: Centrifuge the filtrate for 60 seconds under an environment with a temperature of 40°C and a pressure of 0.2 MPa, discard the precipitate, and use the centrifuge for the next step; wherein, the speed of the centrifuge is 6800 rpm;

[0055] S3. Primary adsorption: load the centrifugate obtained in step S2 on LX-8 resin, absorb EGCG in the centrifugate, and elute with 72% ethanol at a speed of 2m 3 / h, collecting the eluate with a solids content of 5%;

[0056] S4. MVR concentration: put the collected eluate into the MVR concentrator to c...

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Abstract

The invention discloses a method for extracting monomeric EGCG from fresh tea, belonging to the technical field of chemistry of natural products. The method comprises the following concrete steps: S1, soaking; S2, centrifugation; S3, primary adsorption; S4, MVR concentration; S5, CF filtration; S6, secondary adsorption; S7, concentration; S8, rinsing; S9, dehydration; S10, drying; and S11, deironing. According to the invention, fresh tea is used as a raw material; the disadvantages of easy pasting and uneasy filtration during extraction of crushed and sieved dry tea at a high temperature in the prior art is overcome; purified water is used as a medium for direct separation of EGCG monomer, and leaching of a plurality of fat-soluble pesticides is reduced; in the process of purification, an LX-8 resin is used for adsorbing EGCG, and an HZ818 resin is used for adsorbing caffeine; crystallization, rinsing and drying are carried out so as to obtain the EGCG monomer; the obtained EGCG monomer is off-white or white powder, and yield of the monomer reaches about 99%. The method has the advantages of simple operation, high separation efficiency, low cost, a short production period and high product purity and is suitable for industrialized large-scale production.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for extracting monomeric EGCG from fresh tea leaves. Background technique [0002] EGCG, namely epigallocatechin gallate, is a component extracted from green tea. It is the main active and water-soluble component of green tea, accounting for 9-13% of the gross weight of green tea. EGCG is the main component of tea polyphenols in green tea. The composition is also the main component of green tea catechins, which account for 10-15% of the total green tea catechins. The catechins include: gallic acid catechin (Gallocatechin, GC), epigallic acid catechin Epigallocatechin (ECG), epigallocatechin gallate (EGCG), epigallocatechin (EGC) and epicatechin (EC), among which the order of antioxidant activity is EC<ECG<EGC <EGCG. Because of its special stereochemical structure, EGCG is the most effective antioxidant polyphenol in green tea, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 顾峰
Owner CHENGDU WAGOTT BIO TECH
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