Method for extracting high-purity theanine from fresh tea leaves

A technology of fresh tea leaves and theanine, applied in chemical instruments and methods, preparation of carboxylic acid amides, preparation of organic compounds, etc., can solve the problems of low product purity, achieve high separation efficiency, low production cost, and simple operation Effect

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  • Abstract
  • Description
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Problems solved by technology

At present, domestic studies have been carried out on extracting theanine from the tea extract or the residual liquid after extracting tea polyphenols. However, the theanine obtained in the prior art is only a crude product, and the product has low purity. For example, China Patent Publication No. CN1587258A discloses a method for extracting theanine from tea leaves, and the purity of the obtained product is only 10%-30%

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  • Method for extracting high-purity theanine from fresh tea leaves

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Embodiment 1

[0023] Embodiment 1: a kind of method extracting high-purity theanine from fresh tealeaves, it comprises the following steps:

[0024] S1. Constant temperature leaching: put fresh tea leaves in an extractor, add purified water with a temperature >80°C and soak for 2.0 hours at a constant temperature, the weight ratio of fresh tea leaves to purified water is 1:5, and the mixed solution in the extractor Cross 18 mesh sieves to obtain the filtrate;

[0025] S2. Centrifugation: Centrifuge the filtrate for 50 seconds at a temperature of 30°C, a pressure of 0.1 MPa, and a speed of 6500 rpm, and collect the centrifugate;

[0026] S3. Primary membrane concentration: use a reverse osmosis membrane with a rejection rate > 95% to concentrate the centrifugate at a concentration temperature of 30°C until the solid content is ≥ 5%;

[0027] S4. Solvent extraction: the primary membrane concentrate is extracted with ethyl acetate, the volume ratio of the concentrate to ethyl acetate is 1.2:1...

Embodiment 2

[0035] Embodiment 2: a kind of method extracting high-purity theanine from fresh tealeaves, it comprises the following steps:

[0036] S1. Constant temperature leaching: put fresh tea leaves in an extractor, add purified water with a temperature > 80°C and soak for 2.5 hours at a constant temperature, the weight ratio of fresh tea leaves to purified water is 1:8, and the mixed solution in the extractor Cross 24 mesh sieves to obtain the filtrate;

[0037] S2. Centrifugation: Centrifuge the filtrate for 70 seconds at a temperature of 50°C, a pressure of 0.3 MPa, and a rotation speed of 7000 rpm, and collect the centrifugate;

[0038] S3. Primary membrane concentration: use a reverse osmosis membrane with a rejection rate > 95% to concentrate the centrifugate at a concentration temperature of 50°C until the solid content is ≥ 5%;

[0039] S4. Solvent extraction: the primary membrane concentrate is extracted with ethyl acetate, the volume ratio of the concentrate to ethyl acetat...

Embodiment 3

[0047] Embodiment 3: a kind of method extracting high-purity theanine from fresh tealeaves, it comprises the following steps:

[0048] S1. Constant temperature leaching: put fresh tea leaves in an extractor, add purified water with a temperature >80°C and soak for 2.2 hours at a constant temperature, the weight ratio of fresh tea leaves to purified water is 1:6, and the mixed solution in the extractor Pass through a 20-mesh sieve to obtain the filtrate;

[0049] S2. Centrifugation: Centrifuge the filtrate for 60 seconds at a temperature of 35°C, a pressure of 0.2 MPa, and a rotation speed of 6800 rpm, and collect the centrifugate;

[0050] S3. Primary membrane concentration: use a reverse osmosis membrane with a rejection rate > 95% to concentrate the centrifugate at a concentration temperature of 40°C until the solid content is ≥ 5%;

[0051]S4. Solvent extraction: the primary membrane concentrate is extracted with ethyl acetate, the volume ratio of the concentrate to ethyl ...

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Abstract

The invention discloses a method for extracting high-purity theanine from fresh tea leaves, and belongs to the technical field of chemistry of natural products. The method includes constant-temperature digestion, centrifugation, primary membrane concentration, solvent extraction, electrodialysis, membrane dialysis, secondary membrane concentration, vacuum concentration, ethanol extraction, crystallization and microwave drying. The method has the advantages that the method is substituted for conventional methods based on ion exchange adsorption, substantial reagents needed by elution of conventional techniques are reduced due to technological adoption of electrodialysis, and operation equipment is easy to acquire, so that the method is simple to operate, low in production cost and ecologically environment-friendly; experiments prove that the purity of products obtained according to the method can be more than 98% and separation efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for extracting high-purity theanine from fresh tea leaves. Background technique [0002] Amino acid is an important component of tea, and the system is named N-acetyl-γ-L-glutamine (N-ethyl-γ-L-glatamine). Theanine is an effective taste substance in green tea, the content of theanine directly determines the quality of tea: no matter what kind of tea, theanine accounts for more than 50% of the free amino acids in tea, and the content of theanine in dry tea is about 1%-2%. Theanine is the ethylamine inducer of glutamic acid. It is synthesized from glutamic acid and ethylamine in the roots of tea trees, transported to the leaves for storage through the trunk, and converted into catechins under sunlight. In 1950, Mr. Shuto first extracted and refined theanine from tea leaves, and determined its chemical structure. Since then, many scholars ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C237/06C07C231/24
CPCC07C231/04C07C237/06
Inventor 顾峰
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