Preparation method for extracting swertiamarin from Swertia bimaculata

A technology of swerberin and picroside, which is applied in the field of preparation of extracting swerpinomacroside from swert sprout, and achieves the effects of reducing production cost, easy operation, and easy control

Inactive Publication Date: 2011-11-16
唐忠海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At home and abroad, researches have been carried out on the content detection and pharmacology of swertomarin, but there is no report on the small-scale extraction of swertamarin with higher purity.

Method used

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  • Preparation method for extracting swertiamarin from Swertia bimaculata
  • Preparation method for extracting swertiamarin from Swertia bimaculata
  • Preparation method for extracting swertiamarin from Swertia bimaculata

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Grind and dry 100 kg of swert sprouts, add 300 L of 70% ethanol (adjust the pH to about 6.0 with 0.1 mol / L hydrochloric acid), heat and reflux at 45 ° C for 2 h, filter twice with three layers of gauze, filter with a ceramic membrane, and decompress Concentrate to 50L (no ethanol smell), then apply the concentrated solution to a macroporous adsorption resin LSA-20 column, elute with 70% ethanol to obtain the eluate, and concentrate the eluate to dryness. Then heat and dissolve with 50L of 70% ethanol, add 5kg of granular activated carbon for decolorization for 30min, then concentrate to dryness, dissolve in 2L of absolute ethanol, add hot water to mix, add excess petroleum ether, then crystallize at room temperature for 24h, and filter to obtain crystals , and then recrystallized to obtain high-purity swerticroside.

[0031] After testing, the Agilent1100 high-performance liquid chromatograph, the chromatographic column is an Alltimal C18 column (250mm * 4.6m...

Embodiment 2

[0032] Example 2: Grind and dry 150 kg of swert sprouts, add 400 L of 70% ethanol (adjust the pH to about 6.0 with 0.1 mol / L hydrochloric acid), heat and reflux for extraction at 45° C. for 2 h, filter twice with three layers of gauze, filter with a ceramic membrane, and decompress Concentrate to 60L (no ethanol smell), then apply the concentrated solution to a macroporous adsorption resin LSA-20 column, elute with 60% ethanol to obtain the eluate, and concentrate the eluate to dryness. Then heat and dissolve with 70L of 70% ethanol, add 5kg of granular activated carbon for decolorization for 30min, then concentrate to dryness, dissolve in 4L of absolute ethanol, add hot water to mix, add excess petroleum ether, then crystallize at room temperature for 24h, and filter to obtain crystals , and then recrystallized to obtain high-purity swerticroside. Purity testing was carried out according to the method of Example 1, and the purity was 90.17%. The results are shown in Table 1. ...

Embodiment 3

[0033] Example 3: Grind and dry 200 kg of swert sprouts, add 500 L of 70% ethanol (adjust the pH to about 6.0 with 0.1 mol / L hydrochloric acid), heat and reflux at 45 ° C for 2 h, filter twice with three layers of gauze, filter with a ceramic membrane, and decompress Concentrate to 90L (no ethanol smell), then apply the concentrated solution to a macroporous adsorption resin LSA-20 column, elute with 70% ethanol to obtain the eluate, and concentrate the eluate to dryness. Then heat and dissolve with 90L of 70% ethanol, add 8kg of granular activated carbon for decolorization for 30min, then concentrate to dryness, dissolve in 5L of absolute ethanol, add hot water to mix, add excess petroleum ether, then crystallize at room temperature for 24h, and filter to obtain crystals , and then recrystallized to obtain high-purity swerticroside. Purity detection was carried out according to the method of Example 1, and the purity was 92.53%. The results are shown in Table 1.

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Abstract

The invention provides a preparation method for extracting swertiamarin from Swertia bimaculata, belonging to the technical field of natural product chemistry. The preparation method comprises: extracting dried Swertia bimaculata with an ethanol solution, filtering, concentrating under a pressure reduced condition, carrying out resin adsorption, eluting with ethanol, concentrating the eluate under a pressure reduced condition, decolorizing, and crystallizing and recrystallizing to obtain high-purity swertiamarin. Crystallization and recrystallization purifying processes of the swertiamarin are simple and easy to operate and have high purification degree.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a preparation method for extracting swerbromoside from swert sprouts. Background technique [0002] False Chinese Swertia Herb, Latin name Swertia davidi Franch, belongs to Gentianaceae (Gentian-aceae), mainly produced in China, India, Nepal and other countries. Herbs perennial, erect. Stems four-sided, unmyelinated and smooth, single leaves opposite, sessile and lanceolate or long lanceolate. The flowers are bisexual, with small purple spots on the upper part of the corolla lobes, and two large yellowish-brown spots in the middle. The calyx lobes are nearly equal, and each lobes of the corolla have 1-2 glands at the base. The folks are called Shuihuanglian, Shuilingzhi, and Hefeng Grass, fish gall grass, herring gall grass, etc. Tujia people use it to treat lung heat, jaundice, malignant sores, scabies, and acute enteritis. The clinical applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 唐忠海向华
Owner 唐忠海
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