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Ultrasonic-assisted method for extracting dihydroquercetin in sorghum bran

A technology of ultrasonic-assisted extraction and dihydroquercetin, which is applied in the direction of organic chemistry and the like, can solve the problems of short extraction time, destruction, hindering industrial production and application, etc. Effect

Inactive Publication Date: 2017-09-29
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dihydroquercetin is widely found in pinaceae plants, such as maritime pine, larch, and Douglas fir, and is the flavonoid compound with the largest content in larch, but the content of dihydroquercetin in plants is low, generally only 0.3 ~5.7%, these factors make dihydroquercetin more expensive, which further hinders its industrial production and application
Ultrasonic-assisted extraction is a green, environmentally friendly, efficient and energy-saving extraction method that uses the high-speed, strong cavitation effect and stirring effect generated by ultrasonic waves to destroy the cells of medicinal materials, allowing the solvent to penetrate into the cells of medicinal materials and extract active ingredients. Due to the advantages of short time and high extraction efficiency, the method of ultrasonically assisted extraction of dihydroquercetin in sorghum bran has not been reported in the relevant literature so far.

Method used

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  • Ultrasonic-assisted method for extracting dihydroquercetin in sorghum bran

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Experimental program
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Effect test

Embodiment 1

[0017] Take 100g of sorghum bran powder that has been crushed through a 20-mesh sieve, add 1000mL of ethanol aqueous solution with a volume fraction of 10%, and conduct ultrasonic extraction for 5 minutes under the conditions of ultrasonic frequency 25KHz, power 100W, and temperature 25°C. , the filter residue was repeatedly extracted three times, the combined filtrate was concentrated under vacuum to no alcohol smell; the concentrated solution was applied to a macroporous resin chromatographic column (AB-8 macroporous adsorption resin, non-polar, glass column type is Φ3 .0*L100cm), with the same flow rate, wash the column with 2000mL distilled water; Adopt the ethanol aqueous solution elution of 30% by volume fraction, collect dihydroquercetin effective distillate liquid, detect with high performance liquid chromatography (method sees embodiment 3) Concentrate the collected effective distillates under reduced pressure until there is no alcohol smell, let stand at 0°C, precipit...

Embodiment 2

[0019] Take 100g of sorghum bran powder that has been crushed through a 200-mesh sieve, add 5000mL of ethanol aqueous solution with a volume fraction of 95%, and conduct ultrasonic extraction for 60 minutes under the conditions of ultrasonic frequency 25KHz, power 300W, and temperature 70°C. , the filter residue was repeatedly extracted three times, the combined filtrate was concentrated under vacuum and reduced pressure; the concentrated liquid was applied to a macroporous resin chromatographic column (ADS-17 macroporous adsorption resin, non-polar, glass column type Φ3.0* at a flow rate of 3 mL / min) (1100cm), with the same flow rate, wash the column with 2000mL distilled water; adopt the ethanol aqueous solution elution of 95% by volume fraction, collect dihydroquercetin effective distillate liquid, detect with high performance liquid chromatography (method sees embodiment 3); Concentrate the collected effective fractions under reduced pressure until there is no alcohol smell...

Embodiment 3

[0021] This embodiment is an embodiment of the detection method of dihydroquercetin.

[0022] Dihydroquercetin is detected by high-performance liquid chromatography, and the dihydroquercetin product is 290nm in the detection wavelength, and is separated through Agilent ZORBAX SB-C18 column (4.6 * 250mm, 5 μ m) (mobile phase: A phase is 0.1% Acetic acid aqueous solution, phase B is methanol; flow rate is 1mL / min, column temperature is 25°C, gradient elution), the chromatogram is as follows figure 1 Shown, dihydroquercetin appears chromatographic peak at 25.45min place, and under the same chromatographic condition, the retention time of this peak is consistent with the retention time of kaempferin reference substance.

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Abstract

The invention discloses an ultrasonic-assisted method for extracting dihydroquercetin in sorghum bran and belongs to the technical field of natural product chemistry. According to the method, the sorghum bran is utilized as a raw material, and a pure dihydroquercetin product can be obtained by the following steps of ultrasonic-assisted extraction, suction filtration, decompressing concentration, low-temperature standing, centrifugation, separation / enrichment, elution, distillate liquid low-temperature standing, crystallization and recrystallization and drying. The ultrasonic-assisted method disclosed by the invention can be applied to industrial production of high-purity dihydroquercetin, the utilized raw material of the sorghum bran is low in cost and easy to obtain, the ultrasonic-assisted method has the advantages of simple technological process, convenience in operation, low cost and no toxic harmful reagent pollution, and the dihydroquercetin can be applied to bulk pharmaceutical chemicals of medicine.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for ultrasonically assisted extraction of dihydroquercetin from sorghum bran. Background technique [0002] Dihydroquercetin is also called taxifolin and taxifolin. ,3',4'-pentahydroxyflavanone, the molecular formula is C 15 h 12 o 7 , the molecular weight is 304.26. Dihydroquercetin is one of the active ingredients of natural flavonoids, which belongs to the vitamin PP family and has strong antioxidant capacity. It can effectively eliminate excess free radicals from the human body, promote capillary permeability, improve immune function, and reduce cancer formation; it is an excellent natural medicine for preventing cardiovascular diseases, effectively restoring capillary elasticity, and preventing inflammation and tumor formation . At the same time, dihydroquercetin is also a high-efficiency natural food antioxidant, and its antioxid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40
CPCC07D311/32C07D311/40
Inventor 段玉清罗孝平崔洁媚张海晖张迪蔡梅红
Owner JIANGSU UNIV
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