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Antibacterial isoflavonoid compound, and preparation method and application thereof

An antibacterial and isoflavone technology, applied in the field of isoflavone compounds, to achieve the effect of extending shelf life, safe use, and improving hygiene and safety

Active Publication Date: 2017-08-04
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has isolated a kind of isoflavone compound from Radix Puerariae, activity research shows that this compound has better antibacterial activity; This compound is safe, nontoxic, and antibacterial activity is remarkable, and this compound has not seen relevant report so far

Method used

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  • Antibacterial isoflavonoid compound, and preparation method and application thereof
  • Antibacterial isoflavonoid compound, and preparation method and application thereof
  • Antibacterial isoflavonoid compound, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The preparation of embodiment 1-compound

[0029] Pueraria lobata samples were from Nanping, Guangxi, and the variety was Pueraria mirifica. Sampling 2.0 kg of Pueraria lobata root to 30 meshes, extracting 5 times with methanol with a volume concentration of 95%, 24 hours each time, the weight ratio of the extraction solvent and Pueraria lobata root used each time is 2:1, the extracts are combined, filtered and concentrated under reduced pressure Become flowable viscous extractum extractum, obtain extractum 105g.

[0030] After the extract is dissolved in methanol with a weight ratio of 2.0 times, the sample is mixed with 120 g of 100-mesh silica gel, and 0.4 kg of 160-mesh silica gel is packed into a column for silica gel column chromatography. The volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring Merge the same part, wherein, each gradient elution to TLC spot plate without After the point (that is, after the ...

Embodiment 2

[0031] The preparation of embodiment 2-compound

[0032] The Pueraria lobata sample comes from Wanzhou, Chongqing, and the variety is Chaige. The Pueraria lobata sample is sampled 3.5kg, chopped, crushed to 45 mesh, and extracted 4 times with ethanol with a volume concentration of 95%, each time for 48 hours. The weight ratio is 4:1, and the extracts are combined, filtered and concentrated under reduced pressure to become a flowable viscous extract, to obtain 250g of extract.

[0033] After dissolving the extract with 2.0 times the amount of pure methanol by weight, mix the sample with 250g of 80-mesh silica gel, pack 1.0kg of 250-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 1:0, 20:1 , 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring Merge the same part, wherein, each gradient elution to TLC spot plate After no point, replace the next gradient elution to obtain 8 parts, wherein the chloroform-ac...

Embodiment 3

[0034] The preparation of embodiment 3-compound

[0035] Pueraria lobata sample comes from Lincang, Yunnan, and the variety is Chaige root. 5 kg of pueraria lobata is sampled and pulverized to 50 mesh, and extracted 3 times with 75% acetone by ultrasound, each extraction takes 72 hours, and the weight ratio of the extraction solvent used each time to Pueraria lobata root is 4 : 1, the extracts are combined, filtered, concentrated under reduced pressure into a flowable viscous extract, to obtain extract 380g.

[0036]After the extract was dissolved in methanol with a weight ratio of 1.6 times, the sample was mixed with 400 g of 90 mesh thick silica gel, and 1.2 kg of 180 mesh silica gel was packed into a column for silica gel column chromatography, with a volume ratio of 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring Merge the same part, wherein, each gradient elution to TLC spot plate without After the point, the next gradient elut...

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Abstract

The invention relates to an antibacterial isoflavonoid compound, and a preparation method and application thereof, belonging to the technical field of the chemistry of natural products. The compound is named as 8-hydroxy-7-hydroxymethyl-4',6-dimethoxy-isoflavone. Results of activity tests prove that the compound has good bacteriostatic effect. When applied to tobacco liquid for quality preservation, the compound can effectively inhibit the growth of microbes in the tobacco liquid and prolongs the shelf life of the tobacco liquid; and when applied to tipping paper of a cigarette, the compound can eliminate or reduce propagation and reproduction of bacteria in the tipping paper of the cigarette.

Description

technical field [0001] The invention relates to an antibacterial isoflavone compound, in particular to an isoflavone compound extracted for the first time from traditional medicine and food homologous plant kudzu root. At the same time, the invention also relates to the preparation method of the compound, its application in the shelf life of tobacco tobacco liquid, and in the preparation of cigarette antibacterial tipping paper, belonging to the technical field of natural product chemistry. Background technique [0002] Pueraria lobata is the dried root of the leguminous plant Pueraria mirifica, commonly known as Pueraria mirifica. Excavated in autumn and winter, cut into thick slices or small pieces while fresh, and dried. Sweet, pungent, cool. It has the functions of relieving muscle and reducing fever, clearing rash, promoting body fluid and quenching thirst, promoting yang and relieving diarrhea. It is often used for superficial syndromes of fever, stiff neck and back...

Claims

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Application Information

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IPC IPC(8): C07D311/64D21H21/36A01N43/16A01P1/00A01P3/00
Inventor 李雪梅米其利李晶刘欣杨叶昆陈建华夭建华徐济仓张承明孔维松许永周敏杨光宇胡秋芬李干鹏
Owner CHINA TOBACCO YUNNAN IND
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