The invention relates to the field of
chemical treatment of natural medicines, in particular to a method for extracting
swainsonine from barbadosweed endophytic fungi (U.
oxytropis), which comprises the following steps: (1) preparing the barbadosweed endophytic fungi into a bacterial suspension, and culturing; (2) after culture of the bacterial suspension in the step (1) is finished, performing suction
filtration, rinsing,
drying,
grinding and wall breaking, and collecting
dry powder; (3) weighing the
dry powder collected in the step (2), transferring the
dry powder into a
centrifuge tube, adding
methanol, extracting, centrifuging, collecting supernate, evaporating to
dryness, redissolving, filtering and diluting; according to the extraction method, the yield of
swainsonine reaches 220 mu g / g and is remarkably improved compared with the prior art, the method is simple in process condition, safe, low in cost and easy for industrial production, a basis is provided for development and utilization of
swainsonine produced through microbial
fermentation,
raw material resources of swainsonine products are expanded, and the method is suitable for industrial production. Wide application prospects are realized.