Spherosinin degradation bacterium calcium acetate fixed bacillus YLZZ-1 and method for producing the same

A technology of YLZZ-1 and swainsonine, applied in the field of microorganisms

Inactive Publication Date: 2010-06-09
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the biodegradation of locotoxin has not been reported yet, but these research methods can be used for reference. Explore a SW biodegradation pathway, screen out microorganisms that can degrade SW, and transfer the enzyme gene that degrades the toxin into the normal animal gastrointestinal tract. On the flora or animal body, it can not only fundamentally solve the problem of locoweed poisoning in animals, but also bring good economic and environmental benefits, and play a positive role in the biological control of grassland poisonous weeds and the study of grassland ecology

Method used

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  • Spherosinin degradation bacterium calcium acetate fixed bacillus YLZZ-1 and method for producing the same
  • Spherosinin degradation bacterium calcium acetate fixed bacillus YLZZ-1 and method for producing the same
  • Spherosinin degradation bacterium calcium acetate fixed bacillus YLZZ-1 and method for producing the same

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Isolation and screening of swainsonine (SW) degrading bacteria (YLZZ-1 strain)

[0035] 1) Collection of grass samples: locoweeds collected from growing places such as Gansu, Tibet, Inner Mongolia, and Qinghai;

[0036] 2) Soil sample cultivation: bury 1 kg locoweed in the soil at a depth of 10-20 cm, take out the soil around the grass sample after 3-6 months, and fully mix the collected soil samples;

[0037] 3) Isolation and screening of bacterial strains: Take 10 g of well-mixed soil samples and add them to a conical flask filled with 90 mL of sterile water and sterile glass beads, place them on a shaker, shake fully at 200 r / min for 10 min, prepare a bacterial suspension, and then take 10mL bacterial suspension was added to 100mL enriched culture medium, and cultured overnight at 30°C, 180r / min, pH 7.0, and then inoculated into fresh swainsonine (SW) 30mg / Cultivate in the domestication medium of L, transplant once every 4 days later, and gradually incre...

Embodiment 2

[0043] Homology analysis of the 16S rDNA sequence of embodiment 2YLZZ-1 bacterial strain

[0044] The 16S rDNA sequence of the YLZZ-1 strain (SEQ ID NO.1 in the sequence table) was subjected to sequence homology analysis through the Blast retrieval system of NCBI, and a phylogenetic tree was constructed using DNAStar software, see Figure 7 . Depend on Figure 7 It is concluded that the genetic evolution distance of the YLZZ-1 bacterial strain is the closest to Acinetobacter, and it is in the same minimal branch as Acinetobacter calcoaceticus, and it is the same as the known bacterial strain Acinetobacter calcoaceticus strain CAI-13 (DQ257421). The homology reached 100%, indicating that the bacteria belonged to Acinetobacter calcoacetate in molecular phylogenetic taxonomy.

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Abstract

The invention discloses a swainsonine degradation bacteria acetic acid calcium acinetobacter YLZZ-1, which is of short rod shape, 0.2 to 0.3Mum x 0.5 to 1.0Mum, gram-negative without capsule, germ andmotion; bacterial colony is circular, ivory-white, smooth on the surface, wet, elevated and regular in edge and not transparent; bacterial strain is artificially separated and screened out from locoweed growing soil in areas such as Ganshu, Tibet, Inner Mongolia and Qinghai and is preserved at the preservation center for typical culturing in China on Jul., 20, 2007 with preservation No. of CCTCCNO: M 207108. The invention has the advantages of degrading swainsonine which is main toxin of locoweed (Swainsonine, SW).

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a new Acinetobacter calcoaceticus (Acinetobacter calcoaceticus), in particular to a degrading bacterium Acinetobacter calcoaceticus capable of effectively degrading swainsonine (Swainsonine, SW), the main toxin of locoweed YLZZ-1 also relates to the preparation and cultivation method of the degrading bacteria and its application. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, and the damage area is 11 million hm2, accounting for 2.8% of the grassland area in China. Because locoweed has strong adaptability, it grows vigorously on desert grasslands, and turns green ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16A23K10/18
Inventor 王建华赵兴华耿果霞
Owner NORTHWEST A & F UNIV
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