Oxytropis ehrig verticillium FEL5-AS1 of synthesized swainsonine and application thereof
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A technology of FEL5-AS1 and swainsonine, applied in the field of microorganisms, can solve the problems of high price and lack of swainsonine sources
Inactive Publication Date: 2009-08-26
NORTHWEST A & F UNIV
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Swainsonine has good prospects for developing new anti-tumor drugs, but the source of swainsonine is ve
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Embodiment 1
[0018] Embodiment 1: Isolation and screening of fungus Ehrigia spinosa FEL5-AS1
[0019] 1) Wash the whole plant of Astragalus membranaceus grown on the Shigatse grassland in Tibet with deionized water to remove the dust on the surface of Astragalus membranaceus, and use sterilized tweezers to separate the leaves, stems and seeds of the plant, and then separate the leaves, stems and seeds Wrap them with gauze respectively for surface disinfection.
[0020] 2) Use sterilized filter paper to absorb the water on the surface-sterilized plant tissue, and then use sterilized scissors to cut leaves, stems, seeds, etc. into 3mm 2 Then inoculate each tissue piece on the surface of water agar medium with sterilized tweezers, and culture it in an incubator at 18°C.
[0021] 3) When the mycelium grows from around the tissue block, pick the mycelium at the edge of the colony and inoculate it on the surface of the enrichment medium, and place it in an incubator at 18°C for enrichment cul...
Embodiment 2
[0022] Embodiment 2: the identification of fungus Ehrigia spinosa FEL5-AS1
[0023] 1. Morphological features
[0024] The colony of the FEL5-AS1 strain was round, white at first, and gradually changed to light yellow, brown, dark brown with the prolongation of the culture time, with a slight protrusion in the center ( figure 1 ). The mycelium has a transverse septum and no mediastinum. Some old hyphae produce conidiophores with few branches. The spore-forming peduncles are bent knee-like and have obvious spore scars. Conidia and conidiophores with septum, without mediastinum, the septum of conidia is thicker, the number of septa varies from 1 to 4, the spores are rod-shaped, nearly cylindrical, and the size of conidia is 20-90 μm ×5~10μm( figure 2 ).
[0025] 2) Characteristics of culture
[0026] The strain grows very slowly on PDA, PCA, Czapek and other media, and its growth rate is 0.60, 0.51 and 0.48mm / d respectively. The optimum growth temperature is 18°C, and the ...
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Abstract
The invention discloses an oxytropis ehrig verticillium FEL5-AS1 of a synthesized swainsonine which is preserved in China Center for Type Culture Collection on 8th, Oct of 2008, with the preservation number of CCTCC NO: M 2008144. The colony of the bacterial strain is round, which is white at first. With the extension of the culturing time, the colony gradually changes into straw yellow, tan and pitchy, and the center processes a little. Mycelium has transverse layers but has no mediastinum. Part of aged mycelia produces conidiophores which have branches. The conidiophores bend like knee bending, and spore formation scars are obvious. Conidia and conidiophores have transverse layers but have no mediastinum. The diaphragm of the conidia is thick, and the transverse layers consist 1-4. The spores are bar-shaped, similar to column. The size of the conidia is 20-90Mum multiplied by 5-10 Mum. The bacterial strain is obtained from leaves, stems and seeds of astragalus strictus by artificial separation and screening and has the function of synthesizing swainsonine (SW) which is main toxin of locoweed.
Description
technical field [0001] The invention belongs to the technical field of microbes, and relates to a novel Erigeria spinosa (Embellisaoxytropis), in particular to a kind of Embellisaoxytropis capable of synthesizing the main toxin Swainsonine (Swainsonine, SW) of Stemstragalus Bacteria FEL5-AS1 and applications. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of damage reaching 11 million ha 2 , accounting for 2.8% of the country's grassland area. Astragalus membranaceus is a kind of locoweed Astragalus in my country, mainly distributed in Tibet; Astragalus membranaceus mainly harms livestock such as horses, sheep, cattle, and camels. species improveme...
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