Spherosinin degradation phage wheat germ oligotrophy unit cell bacterium YLZZ-2 and separation process thereof

A technology of Stenotrophomonas, YLZZ-2, applied in the field of microorganisms

Inactive Publication Date: 2010-01-06
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the biodegradation of locotoxin has not been reported yet, but these research methods can be used for reference. Explore a SW biodegradation pathway, screen out microorganisms that can degrade SW, and transfer the enzyme gene that degrades the toxin into the normal animal gastrointestinal tract. On the flora or animal body, it can not only fundamentally solve the problem of locoweed poisoning in animals, but also bring good economic and environmental benefits, and play a positive role in the biological control of grassland poisonous weeds and the study of grassland ecology

Method used

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  • Spherosinin degradation phage wheat germ oligotrophy unit cell bacterium YLZZ-2 and separation process thereof
  • Spherosinin degradation phage wheat germ oligotrophy unit cell bacterium YLZZ-2 and separation process thereof
  • Spherosinin degradation phage wheat germ oligotrophy unit cell bacterium YLZZ-2 and separation process thereof

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Embodiment 1

[0037] Embodiment 1: the preparation method of Stenotrophomonas maltophilia YLZZ-2 bacterial strain

[0038] 1) Collection of grass samples: locoweeds collected from growing places such as Gansu, Tibet, Inner Mongolia, and Qinghai;

[0039] 2) Preparation of soil samples: bury 1 kg locoweed in the soil at a depth of 10-20 cm, take out the soil around the grass sample half a year later, and fully mix the collected soil samples;

[0040] 3) Isolation and screening of bacterial strains: Take 10 g of well-mixed soil samples and add them to a conical flask filled with 90 mL of sterile water and sterile glass beads, place them on a shaker, shake fully at 200 r / min for 10 min, prepare a bacterial suspension, and then take Add 10mL of bacterial suspension into 100mL enrichment medium, and culture overnight at 30°C, 180r / min, pH 7.0, and then inoculate 10% of the inoculum into fresh acclimatization medium containing SW 30mg / L After culture, inoculate once every 4 days, and gradually i...

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Abstract

The invention discloses a swainsonine degrading bacteria stenotrophomonas maltophilia YLZZ-2, which is a rod-shaped bacteria and is (0.3 to 0.5) Mum multiplied by (1.3 to 1.5) Mum; the gram stain is negative, and the invention has no capsule and spore but has mobility and a plurality of flagellums; the bacterial colony is circular, light yellow green, smooth in surface, moist, uplift, trim in edge and non-transparent, and when the culture time is long, the invention has a concentric circular semi-transparent inner ring; the bacterial strain is obtained by artificial separation and screening from the soil which buried the locoweed that is grown at Gansu, Tibet, Mongolia, Qinghai and other places, and the invention is collected in the China center for type culture collection on July 20, 2007 with the collection number of CCTCC NO: M 207109. The bacterial strain is characterized in that the invention can degrade the major toxin swainsonine (SW) of locoweed.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a new stenotrophomonas maltophilia, in particular to a degrading bacterium capable of effectively degrading swainsonine (Swainsonine, SW), the main toxin of locoweed. The trophomonas bacterium YLZZ-2 also relates to the isolation, identification, cultivation method and application of the degrading bacterium. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of ​​damage reaching 11 million ha 2 , accounting for 2.8% of the country's grassland area. . Because locoweed has strong adaptability, it grows vigorously on desert grasslands, and turns green earl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16
Inventor 王建华赵兴华耿果霞
Owner NORTHWEST A & F UNIV
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