Oxytropis ehrig sporangium FEL6-AS2 for synthesizing SW and application thereof
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A technology of FEL6-AS2 and swainsonine, applied in the field of microorganisms, can solve the problems of high price and lack of swainsonine sources
Inactive Publication Date: 2009-12-09
NORTHWEST A & F UNIV
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Swainsonine has good prospects for developing new anti-tumor drugs, but the source of swainsonine is ve
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Embodiment 1
[0021] Embodiment 1: Isolation and screening of fungus Ehrigia spinosa FEL6-AS2
[0022] 1) Astragalus membranaceus is collected from the whole stem growing on the Shigatse grassland in Tibet. After cleaning its surface with deionized water, separate the leaves, stems, and species of the plant with sterilized tweezers, and then separate the leaves, stems, and species. Wrap it with gauze for surface disinfection;
[0023] 2) Use sterilized filter paper to absorb the water on the surface-sterilized plant tissue, and then use sterilized scissors to cut the leaves, stems, and seeds into 3mm 2 The size of the tissue pieces, and then use sterilized forceps to inoculate each tissue piece on the surface of the water agar medium, and culture it in the incubator at 18°C;
[0024] 3) When the hyphae grow out from around the tissue block, pick the mycelium at the edge of the colony and inoculate it on the surface of the enrichment medium, and place it in an incubator at 18°C for enrich...
Embodiment 2
[0027] Embodiment 2: the identification of fungus Ehrigia spinosa FEL6-AS2
[0028] 1. Morphological features
[0029] The colony of the FEL6-AS2 strain was round, white at first, and gradually changed to light yellow, brown, dark brown with the prolongation of the culture time, with a slight protrusion in the center ( figure 1 ). The mycelium has a transverse septum without a mediastinum, and some old mycelium give birth to conidiophores with few branches. Conidia and conidiophores have transverse septum without mediastinum. The septum of conidia is thicker and the number of septa varies from 1 to 4. The spores are rod-shaped, nearly cylindrical, and some are oval, divided into The spore size is 20~80μm×6~12μm( figure 2 ).
[0030] 2) Characteristics of culture
[0031] The strain grows very slowly on PDA, PCA, Czapek and other media, and its growth rate is 0.57, 0.49 and 0.47mm / d respectively. The optimum growth temperature is 18°C, and the growth is inhibited when the...
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Abstract
The invention discloses an oxytropis ehrig sporangium FEL6-AS2 for synthesizing SW, which is preserved in the China Center for Type Culture Collection on October 8, 2008, with the preservation serial number of CCTCC No: M 208145. The colony of the strain is circular and is white originally, the color is gradually changed into a grey-white color, a grey-black color and a black color along with the prolongation of the culture time, and the center protrudes. The mycelium has transverse septa and does not have longitudinal septa, part of aged hyphae puts forth conidium peduncles, and the conidium peduncles have branches. Conidia and the conidium peduncles have the transverse septa and do not have the longitudinal septa, the transverse septa of the conidia are thicker, the number of the transverse septa is 1 to 4, sporules are in bar shapes and are approximately cylindrical, parts of the sporules are circular, and the size of the conidia is 20 to 80mum*6 to 12mum; and the strain is obtained from the leaves, stems and seeds of astragalus root with straight stems by artificial separation and screening, and has the function of synthesizing the SW which is the main toxin of locoweed.
Description
technical field [0001] The invention belongs to the technical field of microbes, and relates to a novel Erigeria spinosa (Embellisaoxytropis), in particular to a kind of Embellisioxytropis capable of synthesizing the locotoxin-swainsonine (Swainsonine, SW). Bacteria FEL6-AS2 also relates to the use of the strain. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of damage reaching 11 million ha 2 , accounting for 2.8% of the country's grassland area. Astragalus membranaceus is a kind of locoweed Astragalus in my country, mainly distributed in Tibet; Astragalus membranaceus mainly harms livestock such as horses, sheep, cattle, and camels. kind of impro...
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