Technique for spherosinin purification with continuous column chromatography

A technology of swainsonine and column chromatography, which is applied in the field of natural product chemistry research, can solve the problems of low yield, complex process and high cost, and achieve the effects of high extraction rate, simple process flow and low cost.
CN101270118AInactive Publication Date: 2008-09-24贺武利 +3
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
贺武利
Publication Date
2008-09-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

Belonging to the field of chemical analyses on natural products, the present invention relates to a method for extracting, separating, purifying and testing natural products, in particular to a technique for extracting swainsonine by continuous column chromatography. The present invention resolves the defects of the prior art, including a complex process, high cost and low yield rate. The solution orderly includes the following steps: (1) the preprocessing of locoweed; (2) the acquisition of locoweed extract; (3) the preparation of a crude product: The extract is roughly separated by polar macroporous adsorption resin and, after TLC test, merged with eluant containing swainsonine to serve as the crude product for later use; (4) the purification of the swainsonine: The crude product is purified by silica gel column chromatography, and by the TLC test and the GC test, the parts of the swainsonine, which have relative content larger than or equal to 10 percent, are respectively collected; (5) the acquisition of a pure swainsonine product by the HPLC test; the parts collected in the step (4) undergo the HPLC test in order to collect eluate in the appearance time period of the swainsonine, and after the solvent is evaporated, the pure swainsonine product can be produced by recrystallization.
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Description

1. Technical field

[0001] The invention belongs to the field of chemical research of natural products, and in particular relates to extraction, separation, purification and detection methods of natural products, in particular to a process for extracting swainsonine by continuous column chromatography. 2. Background technology

[0002] Locoweed is a very common weed in Northwest my country. It is easy to cause poisoning after being eaten by livestock, and it is often regarded as a harmful weed. Studies in recent years have shown that the main toxic component of locoweed - swainsonine, can not only inhibit the growth of cancer cells, but also activate the body's immunity and reduce the side effects of cancer patients caused by chemotherapy. Therefore, it is a promising natural medicine.

[0003] There are three main sources of swainsonine (SW): ① Extraction and separation from plants. The currently known plants containing swainsonine are mainly Astragalus and Oxytopis plants...

Claims

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