Adipose transplantation material containing highly concentrated adipose-derived stem cells and extracellular matrix, and pure physical preparation method and application thereof

A fat-derived, highly concentrated technology, applied in the field of fat transplantation and stem cell therapy, can solve the problems of high cost, complicated separation process, limited capacity, etc., and achieve the effect of low cost, rich source, and no rejection.

Active Publication Date: 2016-12-07

鲁峰

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AI-Extracted Technical Summary

Problems solved by technology

[0003] However, there are many limitations in the application of SVF, for example: 1. After transplantation, because SVFs are out of the microenvironment under the physiological state, as a cell suspension, their ability to colonize the target tissue i...

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Abstract

The invention discloses an adipose transplantation material containing highly concentrated adipose-derived stem cells and an extracellular matrix (ECM), and a pure physical preparation method and an application thereof; with application of a fluid vortex principle, most of mature adipocytes in an adipose tissue are broken; at the same time, with application of a flocculation sedimentation technology, oil droplets generated from breaking the adipocytes are gathered and removed. A series of mechanical processing processes are adopted, the pure physical means is adopted, and no any exogenous substances are added. The multiple physical methods are used for removing the mature adipocytes and grease affecting the tissue retention rate after transplantation. In a prepared SVF-gel, the adipose-derived stem cells and endothelial progenitor cells are highly concentrated; the concentrated stromal vascular fraction cells (SVFs) still adhere on the ECM, the number of stem cells fix-planted in local after transplantation is far greater than the ordinary adipose transplantation number; as a stem cell therapy method, the adipose transplantation material has better long-term curative effect.

Application Domain

Prosthesis

Technology Topic

Fat graftingOil droplet +15

Image

Examples

Experimental program(3)
Effect test(1)

Example Embodiment

[0044] Example 1

[0045] 1) Treat the obtained granular fat with Coleman technology: centrifuge at 1200g/min for 3 minutes, recover the upper layer of fat after centrifugation, discard the bottom layer, save the middle layer of high-density adipose tissue, transfer it to a needleless syringe, and use scissors Further cut the fat tissue.

[0046] The above-mentioned granular fat is obtained by wet liposuction technology, which uses a liposuction needle to suck adipose tissue from the patient's abdomen or thigh.

[0047] 2) Connect the syringe with fat in the previous step and a new syringe through the luer connector (such as figure 1 Shown) Connect the injection ports of the two syringes to achieve a sealed butt, push the plunger of the syringe containing adipose tissue, and inject the adipose tissue into the contralateral syringe through the connector at a bolus speed of 20ml/s, and push back and forth continuously. Note 2min (such as figure 2 Shown).

[0048] 3) Take the remaining fat in step 1) and mix it into the fat tissue repeatedly injected in the previous step;

[0049] 4) Pass the adipose tissue obtained in step 3) through Tulip’s Nano Transfer (such as image 3 (Shown) filter (the filter pore size is not greater than 0.2mm), put the filtrate into a sealed syringe, pull off the syringe plug, maintain the negative pressure in the syringe, and then slightly shake for 30 seconds; flocculation appears during the slight shaking process, showing It is like egg drop soup; centrifuge the shaken mixture at 1800~2200g/min for 3~5min.

[0050] 5) After step 4) centrifugation, the mixture is separated into layers, the top layer is grease, the bottom layer is swelling liquid, and the volume of the grease layer accounts for more than 80% of the total volume of the mixture, then the intermediate layer product is taken to obtain SVF-gel, which is the height Concentrated fat-derived stem cells and extracellular matrix transplantation materials;

[0051] If the mixture does not stratify after centrifugation, or the volume of the top oil layer accounts for less than 80% of the volume of the whole mixture, repeat steps 2) to 4) of the mixture for no more than 3 times.

[0052] The SVF-gel prepared above can be used for facial rejuvenation and soft tissue damage repair. The SVF-gel graft material is directly placed in a syringe, and intradermal injection or local precision injection filling can be performed through a 27G fine needle.

Example Embodiment

[0053] Example 2

[0054] 1) Treat the obtained granular fat with Coleman technology: centrifuge at 800g/min for 5 minutes, recover the upper layer of fat after centrifugation, discard the bottom layer, save the middle layer of high-density adipose tissue, transfer it to a needleless syringe, and use scissors Further cut the fat tissue.

[0055] The above-mentioned granular fat is obtained by wet liposuction technology, which uses a liposuction needle to suck adipose tissue from the patient's abdomen or thigh.

[0056] 2) Connect the syringe with fat in the previous step and a new syringe through the Luer connector to connect the injection ports of the two syringes to achieve a sealed docking, push the syringe plunger containing the fat tissue, and inject the fat tissue through the connector In the contralateral syringe, the bolus speed is 10ml/s, and the bolus is continuously back and forth for 1 min.

[0057] 3) Take the remaining fat in step 1) and mix it into the fat tissue that has been repeatedly injected in the previous step;

[0058] 4) Filter the adipose tissue obtained in step 3) through Nano Transfer of Tulip Company (the filter pore size is not greater than 0.2mm), put the filtrate into a sealed syringe, pull off the syringe plug, maintain the negative pressure in the syringe, and then slightly shake 45 seconds; flocculation appears in the process of slight shaking, which is like egg flower soup; centrifuge the shaking mixture at 1800g/min for 3min.

[0059] 5) After step 4) centrifugation, the mixture is separated into layers, the top layer is grease, the bottom layer is swelling liquid, and the volume of the grease layer accounts for 80% or more of the volume of the whole mixture, then the intermediate layer product is taken to obtain SVF-gel, which is Highly concentrated adipose-derived stem cells and extracellular matrix transplantation materials;

[0060] If the mixture does not stratify after centrifugation, or the volume of the top oil layer accounts for less than 80% of the volume of the whole mixture, repeat steps 2) to 4) of the mixture for no more than 3 times.

[0061] The SVF-gel prepared above can be used for facial rejuvenation and soft tissue damage repair. The SVF-gel graft material is directly placed in a syringe, and intradermal injection or local precision injection filling can be performed through a 27G fine needle.

Example Embodiment

[0062] Example 3

[0063] 1) Treat the obtained granular fat with Coleman technology: centrifuge at 1200g/min for 5 minutes, recover the upper layer of fat after centrifugation, discard the bottom layer, save the middle layer of high-density adipose tissue, transfer it to a needleless syringe, and use scissors Further cut the fat tissue.

[0064] The above-mentioned granular fat is obtained by wet liposuction technology, which uses a liposuction needle to suck adipose tissue from the patient's abdomen or thigh.

[0065] 2) Connect the syringe with fat in the previous step and a new syringe through the Luer connector to connect the injection ports of the two syringes to achieve a sealed docking, push the syringe plunger containing the fat tissue, and inject the fat tissue through the connector In the contralateral syringe, the bolus speed was 20ml/s, and the bolus injection continued for 1 min.

[0066] 3) Take the remaining fat in step 1) and mix it into the fat tissue that has been repeatedly injected in the previous step;

[0067] 4) Filter the adipose tissue obtained in step 3) through Nano Transfer of Tulip Company (the filter pore size is not greater than 0.2mm), put the filtrate into a sealed syringe, pull off the syringe plug, maintain the negative pressure in the syringe, and then slightly shake 25 seconds; flocculation occurs during the slight shaking process, which is like egg flower soup; centrifuge the shaking mixture at 2200g/min for 5min.

[0068] 5) After step 4) centrifugation, the mixture is separated into layers, the top layer is grease, the bottom layer is swelling liquid, and the volume of the grease layer accounts for 80% or more of the volume of the whole mixture, then the intermediate layer product is taken to obtain SVF-gel, which is Highly concentrated adipose-derived stem cells and extracellular matrix transplantation materials;

[0069] If the mixture does not stratify after centrifugation, or the volume of the top oil layer accounts for less than 80% of the volume of the whole mixture, repeat steps 2) to 4) of the mixture for no more than 3 times.

[0070] The SVF-gel prepared above can be used for facial rejuvenation and soft tissue damage repair. The SVF-gel graft material is directly placed in a syringe, and intradermal injection or local precision injection filling can be performed through a 27G fine needle.

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