Adipose transplantation material containing highly concentrated adipose-derived stem cells and extracellular matrix, and pure physical preparation method and application thereof
A fat-derived, highly concentrated technology, applied in the field of fat transplantation and stem cell therapy, can solve the problems of high cost, complicated separation process, limited capacity, etc., and achieve the effect of low cost, rich source, and no rejection.
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[0044] Example 1
[0045] 1) Treat the obtained granular fat with Coleman technology: centrifuge at 1200g / min for 3 minutes, recover the upper layer of fat after centrifugation, discard the bottom layer, save the middle layer of high-density adipose tissue, transfer it to a needleless syringe, and use scissors Further cut the fat tissue.
[0046] The above-mentioned granular fat is obtained by wet liposuction technology, which uses a liposuction needle to suck adipose tissue from the patient's abdomen or thigh.
[0047] 2) Connect the syringe with fat in the previous step and a new syringe through the luer connector (such as figure 1 Shown) Connect the injection ports of the two syringes to achieve a sealed butt, push the plunger of the syringe containing adipose tissue, and inject the adipose tissue into the contralateral syringe through the connector at a bolus speed of 20ml / s, and push back and forth continuously. Note 2min (such as figure 2 Shown).
[0048] 3) Take the remaining...
Example Embodiment
[0053] Example 2
[0054] 1) Treat the obtained granular fat with Coleman technology: centrifuge at 800g / min for 5 minutes, recover the upper layer of fat after centrifugation, discard the bottom layer, save the middle layer of high-density adipose tissue, transfer it to a needleless syringe, and use scissors Further cut the fat tissue.
[0055] The above-mentioned granular fat is obtained by wet liposuction technology, which uses a liposuction needle to suck adipose tissue from the patient's abdomen or thigh.
[0056] 2) Connect the syringe with fat in the previous step and a new syringe through the Luer connector to connect the injection ports of the two syringes to achieve a sealed docking, push the syringe plunger containing the fat tissue, and inject the fat tissue through the connector In the contralateral syringe, the bolus speed is 10ml / s, and the bolus is continuously back and forth for 1 min.
[0057] 3) Take the remaining fat in step 1) and mix it into the fat tissue that ...
Example Embodiment
[0062] Example 3
[0063] 1) Treat the obtained granular fat with Coleman technology: centrifuge at 1200g / min for 5 minutes, recover the upper layer of fat after centrifugation, discard the bottom layer, save the middle layer of high-density adipose tissue, transfer it to a needleless syringe, and use scissors Further cut the fat tissue.
[0064] The above-mentioned granular fat is obtained by wet liposuction technology, which uses a liposuction needle to suck adipose tissue from the patient's abdomen or thigh.
[0065] 2) Connect the syringe with fat in the previous step and a new syringe through the Luer connector to connect the injection ports of the two syringes to achieve a sealed docking, push the syringe plunger containing the fat tissue, and inject the fat tissue through the connector In the contralateral syringe, the bolus speed was 20ml / s, and the bolus injection continued for 1 min.
[0066] 3) Take the remaining fat in step 1) and mix it into the fat tissue that has been ...
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