Method for detecting content of swainsonine in locoweed endophytic fungi

A technology of swainsonine and endophytic fungi, which is applied in the field of analytical chemistry, can solve the problems of limited application, lack of luminescent groups in swainsonine, and difficult detection of swainsonine, and achieve strong specificity and high sensitivity , good reproducibility
CN102590378BInactive Publication Date: 2013-09-11NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2013-09-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for detecting the content of swainsonine in locoweed endophytic fungi. The method comprises the steps: pre-treating an endophytic fungi hyphae sample to prepare a test solution; using a high performance liquid chromatograph evaporative light-scattering detector to detect the swainsonine in the locoweed endophytic fungi; taking a mixed solution of acetonitrile and ammonium acetate buffer solution as a mobile phase, wherein the volume ratio of acetonitrile to ammonium acetate is 0.2-2 to 0.2-2; separating the swainsonine from other materials in the test sample by an HILIC (Hydrophilic Interaction Chromatography) column; and constructing a map by logarithm of swainsonine in different concentrations and logarithm of a corresponding response peak area so as to obtain a standard curve of the swainsonine. According to the method, the swainsonine in the endophytic fungi Undifilum oxytropis can be effectively separated, and the method can be directly applied to the quantitative analysis of the swainsonine. The method is good in separation effect, strong in specificity and high in accuracy, and the analysis method is reliable.
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Description

technical field

[0001] The invention belongs to the technical field of analytical chemistry, and relates to the separation and determination of swainsonine in endophytic fungi, in particular to a method for determining the content of swainsonine in locoweed endophytic fungi. Background technique

[0002] Swainsonine (SW) is an indolizidine alkaloid with the chemical name 1,2,8-trihydroxy-8-hydro-indolizidine. SW is the main toxic component in locoweed (a general term for poisonous plants of the legume genus Oxytropis and Astragalus). Locoweeds are widely distributed around the world, and animals can cause poisoning and even death after eating a large amount of them.

[0003] Swainsonine is not only a plant-derived toxin, but it also has the effect of inhibiting the growth and metastasis of tumor cells. Because SW is a very strong α-mannosidase inhibitor, it can not only inhibit the expression of glycoproteins on the surface of tumor cells, but also inhibit the growth and m...

Claims

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