The invention relates to a rapid detection method for candida albicans by loop-mediated isothermal amplification. Reagents used by the method comprises a loop-mediated isothermal amplification reaction solution, a Bst DNA polymerase and a color development reagent, wherein the reaction solution contains reaction buffer, dNTP, magnesium sulfate, an upstream inner primer 5-CACATTTTGGATAAGGTTCACCATT-ATAGAAACCTCAAGATTTCCGT-3, a downstream inner primer 5-TCGTGTTCGTGAAAGTGAAGATAAT-CTTTCTATCATCCAAATCGTAGA-3, an upstream outer primer 5-ACCGTTGATTTCCAATTCG-3, a downstream outer primer 5-TTAACTTGAGCCATGGAGAT-3, and glycine betaine. The method for detection of candida albicans comprises the steps of DNA extraction of the fungi, loop-mediated isothermal amplification of the candida albicans, and chromogenic detection. The method has the characteristics of rapidness, strong specificity, high sensitivity and low cost.