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Method for extracting rubusoside from sweet tea leaves

A technology of sweet tea and sweet tea, which is applied in the field of natural product chemistry, can solve problems such as the limitation of sweet tea in food, and achieve the effects of reducing production costs, high purification efficiency, and easy operation

Inactive Publication Date: 2011-11-16
JISHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The separation and extraction, testing and pharmacology of sweet tea at home and abroad have been studied, and there are also reports on small-scale extraction of sweet tea with high purity. However, a large number of toxic reagents are used for separation and purification in the extraction and separation process. Significant restrictions on food

Method used

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  • Method for extracting rubusoside from sweet tea leaves
  • Method for extracting rubusoside from sweet tea leaves
  • Method for extracting rubusoside from sweet tea leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Grind and dry 100 kg of sweet tea, add 300 L of 45% ethanol, heat and reflux at 65 ° C for 2 h, filter twice with three layers of gauze, filter with a ceramic membrane, concentrate under reduced pressure to 50 L (no ethanol smell), and then add large pores to the concentrate Adsorbed resin LSA-40 column, eluted with 70% ethanol to obtain the eluate, and concentrated the eluate to dryness. Then heat and dissolve with 80L of 70% ethanol, add 5kg of granular activated carbon for decolorization for 30min, then concentrate to dryness, heat and dissolve with 2L of 90% ethanol, add hot water and mix until the final concentration of ethanol is 50%, then crystallize at room temperature for 24h, filter Crystals were obtained, and then recrystallized to obtain sweet tea samples.

[0028] After testing, the Agilent1100 high performance liquid chromatograph, the chromatographic column is Hypersil ODS (150mm×4.6mm, 5μm), the mobile phase is 65% methanol aqueous solution; t...

Embodiment 2

[0030] Crush and dry sweet tea 500kg, add 45% ethanol 600L, heat and reflux at 65°C for 2 hours, filter twice with three layers of gauze, filter with ceramic membrane, concentrate under reduced pressure to 100L (no ethanol smell), and then apply macroporous adsorption resin LSA- 40 columns, eluted with 70% ethanol to obtain the eluate, and concentrated the eluate to dryness. Then heat and dissolve with 150L of 70% ethanol, add 15kg of activated carbon to decolorize for 30min, then concentrate to dryness, heat and dissolve with 6L of 90% ethanol, add hot water until the final concentration of ethanol is 47%, crystallize at room temperature for 24h, filter to obtain crystals, and then Carry out recrystallization to obtain sweet tea element sample. Purity detection was carried out according to the method of Example 1, and the purity was 97.82%. The results are shown in Table 1.

Embodiment 3

[0032] Crush and dry 200kg of sweet tea, add 200L of 45% ethanol, heat and reflux at 65°C for 2 hours, filter twice with three layers of gauze, filter with ceramic membrane, concentrate under reduced pressure to 50L (no ethanol smell), and then apply macroporous adsorption resin LSA- 40 columns, eluted with 70% ethanol to obtain the eluate, and concentrated the eluate to dryness. Then heat and dissolve with 100L of 70% ethanol, add 8kg of activated carbon to decolorize for 30min, then concentrate to dryness, heat and dissolve with 3L of 90% ethanol, add hot water until the final concentration of ethanol is 40%, crystallize at room temperature for 24h, filter to obtain crystals, and then Carry out recrystallization to obtain sweet tea element sample. Purity detection was carried out according to the method of Example 1, and the purity was 98.95%. The results are shown in Table 1.

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Abstract

The invention provides a method for extracting rubusoside from sweet tea leaves, belonging to the technical field of natural product chemistry. The method comprises the following steps: drying the sweet tea leaves; extracting with ethanol solution, filtering, carrying out pressure reduced concentrating, then absorbing with resin, eluting with ethanol; carrying out pressure reduced concentrating on the elution solution, decoloring, crystallizing and recrystallizing to prepare into the pure rubusoside product. The processes for crystallizing, recrystallizing and purifying rubusoside are simple and easy to operate and ensure high purification degree.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for extracting sweet tea element from sweet tea leaves. Background technique [0002] Sweet tea is a perennial vine of Rosaceae. It is a wild sweet plant unique to Guangxi. It is mainly produced in Dayao Mountain in our region. Because of its sweet taste, it is named sweet tea. Together with Mangosteen and Stevia, it is known as the three major sweet plants in Guangxi. It has a long history of application among the folks in our region. For a long time, local people have been drinking it as tea, and also used it as a substitute for sugar to process food, and it can also be used as medicine. In 1979, the Guangxi Analysis and Testing Research Center conducted an analysis of the chemical composition of sweet tea, and determined that the chemical structure of its sweetening substances was consistent with that of stevioside, and its sweetness was ...

Claims

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Application Information

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IPC IPC(8): A23L1/09
Inventor 唐忠海
Owner JISHOU UNIVERSITY
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