Isoflavone compound with antioxidant activity, preparation method and application thereof

A technology of antioxidant activity and isoflavones, applied in application, food science, organic chemistry, etc., to prevent rancidity, simple structure, and prolong shelf life.

Active Publication Date: 2017-07-28
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The compound is safe, non-toxic, and has significant antioxidant a

Method used

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  • Isoflavone compound with antioxidant activity, preparation method and application thereof
  • Isoflavone compound with antioxidant activity, preparation method and application thereof
  • Isoflavone compound with antioxidant activity, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1-compound preparation

[0026] Take 2.6 kg of dried Pueraria lobata, coarsely crush it to 30 mesh, and extract 4 times with acetone ultrasonically with a volume concentration of 70%. The weight of the organic solvent is 3 times the weight of Pueraria lobata, for 60 minutes each time, combine the extracts, filter , The filtrate was concentrated under reduced pressure until precipitation was observed, left to stand for 3 hours, the precipitate was filtered off, and concentrated into a flowable viscous extract 62g.

[0027] Add 120g of acetone to the extract to dissolve the extract, then add 62g of 100 mesh silica gel to mix the sample, after mixing the sample, use 400g of 200 mesh silica gel to pack the column; the volume ratio is 1:0, 9:1, 8:2, 7:3, 1:1, 3:7 chloroform-acetone mixed organic solvent gradient elution, collected gradient eluent, concentrated, monitored by TLC, combined the same parts to obtain 6 parts A-F, among them, the collected Part 12g of ...

Embodiment 2

[0028] Embodiment 2-compound preparation

[0029] Take 2.8kg of dried Pueraria lobata pieces, coarsely crush them to 35 mesh, and ultrasonically extract 3 times with 95% ethanol at a volume concentration each time. The weight of the organic solvent is 4 times the weight of Pueraria lobata, for 50 minutes each time, combine the extracts, filter , The filtrate was concentrated under reduced pressure until precipitation was observed, left to stand for 4 hours, the precipitate was filtered off, and concentrated into a flowable viscous extract 66g.

[0030] Add 140g of acetone to the extract to dissolve, then add 70g of 100 mesh silica gel to mix the sample, after mixing the sample, use 400g of 200 mesh silica gel to pack the column; the volume ratio is 1:0, 9:1, 8:2, 7:3, 1:1, 3:7 chloroform-acetone mixed organic solvent gradient elution, collected gradient eluent, concentrated, monitored by TLC, combined the same parts to obtain 6 parts A-F, among them, the collected Part 12g of...

Embodiment 3

[0031] Embodiment 3-compound preparation

[0032] Take 2.8kg of dried Pueraria lobata pieces, coarsely crush them to 35 mesh, and ultrasonically extract 5 times each time with methanol with a volume concentration of 95%. The weight of the organic solvent is 5 times the weight of Pueraria lobata root, each time for 50 minutes, combine the extracts, filter , The filtrate was concentrated under reduced pressure until a precipitate was observed, left to stand for 4 hours, the precipitate was filtered off, and concentrated into a flowable viscous extract 60g.

[0033] Add 140g of acetone to the extract to dissolve, then add 70g of 100 mesh silica gel to mix the sample, after mixing the sample, use 400g of 200 mesh silica gel to pack the column; the volume ratio is 1:0, 9:1, 8:2, 7:3, 1:1, 3:7 chloroform-acetone mixed organic solvent gradient elution, collected gradient eluent, concentrated, monitored by TLC, combined the same parts to obtain 6 parts A-F, among them, the collected ...

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Abstract

Belonging to the technical field of natural product chemistry, the invention relates to an isoflavone compound with antioxidant activity, a preparation method and application thereof. The isoflavone compound is separated from the traditional Chinese medicinal material kudzuvine root, and is named as: 4', 8-dihydroxy-7-hydroxymethyl-6-methoxy-isoflavone, and the molecular formula is C17H14O6. Antioxidant activity experiments are carried out on the compound, and the result shows that the compound has strong antioxidant activity. At the same time, the compound can be used as a tobacco feed liquid additive, and can effectively prevent rancidity of tobacco feed liquid and prolong the shelf life.

Description

technical field [0001] The invention relates to an isoflavone compound with antioxidant activity, in particular to an isoflavone compound with antioxidant activity extracted from kudzu root for the first time. Simultaneously, the invention also relates to a preparation method of the compound and its application as a tobacco material liquid additive, belonging to the technical field of natural product chemistry. Background technique [0002] Pueraria lobata is the dried root of the leguminous plant Pueraria mirifica, commonly known as Pueraria mirifica. Excavated in autumn and winter, cut into thick slices or small pieces while fresh; dry. Sweet, pungent, cool. It has the functions of relieving muscle and reducing fever, clearing rash, promoting body fluid and quenching thirst, promoting yang and relieving diarrhea. It is often used for superficial syndromes of fever, stiff neck and back pain, opaque measles, fever and thirst, yin deficiency and thirst, heat diarrhea and d...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40A24B15/40
CPCA24B15/403C07D311/36C07D311/40
Inventor 李雪梅李晶刘欣米其利杨叶昆陈建华夭建华徐济仓张承明孔维松许永周敏杨光宇胡秋芬李干鹏
Owner CHINA TOBACCO YUNNAN IND
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