Method for preparing 1-deoxynojirimycin by membrane separation technology

A deoxynojirimycin and membrane separation technology, which is applied in the field of preparing 1-deoxynojirimycin by membrane separation technology, can solve serious pollution problems, achieve good product quality, reduce the generation of pollutants, and reduce energy consumption.

Inactive Publication Date: 2012-01-11
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As mentioned above, in the existing method for preparing 1-deoxynojirimycin, the use of ion exchange resin in the production process will produce a large amount of acidic and alkaline water waste, which is seriously polluted

Method used

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  • Method for preparing 1-deoxynojirimycin by membrane separation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Fresh mulberry leaves are dried, crushed, weighed 10kg, put into an extraction tank, add 8 times the amount of deionized water and soak at 50°C for 5 hours, extract 3 times, filter the soaking solution and add a hollow polyamide fiber nanofiltration membrane system with a molecular weight cut-off of 300 to filter, Add an appropriate amount of deionized water for dialysis, add hollow polyamide fiber reverse osmosis membrane to concentrate the permeated liquid, add ethyl acetate to extract the concentrated solution for 3 times, concentrate the aqueous phase under reduced pressure, and dry in vacuum to obtain 21 products, of which 1-deoxynojiri Mycin content is 83.4%.

Embodiment 2

[0019] Fresh mulberry leaves are dried, crushed, weighed 50kg, put into an extraction tank, add 10 times the amount of deionized water and soak at 60°C for 3 hours, extract twice, filter the soaking liquid and add a hollow polyethersulfone nanofiltration membrane system with a molecular weight cut-off of 500, Add an appropriate amount of deionized water for dialysis, add hollow polyethersulfone reverse osmosis membrane to concentrate the permeated liquid, add ethyl acetate to extract the concentrated solution 4 times, concentrate the aqueous phase under reduced pressure, and dry in vacuum to obtain 112 products, of which 1-deoxynojiri Mycin content is 79.3%.

Embodiment 3

[0021] Fresh mulberry leaves are dried, crushed, weighed 100kg, put into the extraction tank, add 10 times the amount of deionized water and soak at 50°C for 4 hours, extract twice, filter the soaking liquid and add a hollow polytetrafluoroethylene nanofiltration membrane system with a molecular weight cut off of 400 to filter , add an appropriate amount of deionized water for dialysis, and then add a hollow composite reverse osmosis membrane to concentrate the permeated liquid. The concentrated solution is extracted 3 times with ethyl acetate, the aqueous phase is concentrated under reduced pressure, and vacuum-dried to obtain 232 products, of which 1-deoxynojiri The element content is 80.1%.

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Abstract

The invention belongs to the field of natural product chemistry, and relates to a method for preparing 1-deoxynojirimycin by a membrane separation technology. The method comprises the following steps of collecting fresh mulberry leaves, drying, crushing, adding deionized water into the crushed mulberry leaves for soaking, adding the soak into a nanofiltration membrane system with filtration to carry out filtration, adding the filtrate into a reverse osmosis membrane to carry out concentration, adding ethyl acetate into the concentrated filtrate to carry out extraction, carrying out pressure-reducing concentration of an aqueous phase obtained by the previous step, and carrying out vacuum drying to obtain the desired product. The method for preparing 1-deoxynojirimycin by a membrane separation technology has the advantages of simple process, good operability, low energy consumption, less pollution and good applicability for industrialized production.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, in particular to a method for preparing 1-deoxynojirimycin by membrane separation technology. Background technique [0002] 1-Deoxynojirimycin (1-DNJ), chemical name: 3,4,5-trihydroxy-2-hydroxymethyltetrahydropyridine, molecular formula: C 6 h 13 NO 4 , molecular weight: 163.17, molecular structure: [0003] [0004] 1-Deoxynojirimycin is a polar alkaloid, easily soluble in water, and an effective α-glucosidase inhibitor. It has a significant hypoglycemic effect, can inhibit tumor metastasis and has antiviral effects. It exists in mulberry, commelina, hyacinth and Adenophora, with the highest content in mulberry, with a content of about 0.2% in mulberry leaves. [0005] The existing extraction of 1-deoxynojirimycin is mostly extracted from mulberry leaves, and generally separated by resin column. Such as the patent (application number: 200710067498.3) "A method for extracting 1-de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D211/46
CPCY02P20/10
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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