78 results about "Deoxynojirimycine" patented technology

The present invention provides a method for increasing the activity of a mutant or wild-type α-glucosidase enzyme in vitro and in vivo by contacting the enzyme with a specific pharmacological chaperone which is a derivative of 1-deoxynojirimycin. The invention also provides a method for the treatment of Pompe disease by administration of chaperone small molecule compound which is a derivative of 1-deoxynojirimycin. The 1-deoxynojirimycin derivative is substituted at the N or C1 position. Combination therapy with replacement α-glucosidase gene or enzyme is also provided.

The present invention relates to a mulberry leaf effective component composition for reducing blood sugar and its preparation method. Said mulberry leaf effective component composition contains 20-80% of mulberry leaf polysaccharide and 80-20% of mulberry leaf alkaloid in which 1-deoxynojirimycin content is greater than 50%. Said preparation method includes the following steps: removing impurity, drying, pulverizing to obtain mulberry leaf powder, using organic solvent to make extraction, filtering, removing residue, using hot water to make extraction, filtering, adding ethyl alcohol into filtrate to make its concentration be 40%-80%, filtering to obtain supernatant fluid and precipitate, using cation exchange resin and anion exchange resin to make separation and remove impurity, concentrating and drying so as to obtain mulberry leaf alkaloid.

The invention discloses sanggenone C and sanggenone D extracted from white mulberry root-barks, mulberry twigs and folium leaves and a new medicine application of a composition comprising the sanggenone C and the sanggenone D which are main ingredients, particularly the application of the single ingredient sanggenone C or sanggenone D and the composition composed of the sanggenone C, the sanggenone D, mulberrin, mulberroside, oxidized resveratrol, resveratrol and deoxynojirimycin in preparation of alpha-glucosidase inhibitor medicines. The composition is prepared by respectively extracting medicinal materials containing the chemical components above and blending according to certain proportions, or using several extraction methods for extracting the medicinal materials simultaneously containing the chemical components above. The white mulberry root-barks, the mulberry twigs and the folium leaves are accidentally found to contain the sanggenone C and the sanggenone D apart from containing alkaloids such as deoxynojirimycin; and the sanggenone C and the sanggenone D have stronger pharmacological activity than the alkaloids such as deoxynojirimycin.

A 1-deoxynojirimycin preparing method suitable for industrial production comprises the following steps: (1) raw material pre-treatment: crushing the dry mulberry leaf raw material; (2) enzymelysis: adding water into powder, raising the temperature, adding a combined enzyme for enzymelysis, and filtering; (3) continuous combined extraction: performing continuous combined extraction on filter residues by using 3-4 extracting devices, mixing extracting solutions, and filtering; (4) alcohol precipitation and centrifugation: performing pressure-reduction concentration to 1/3-1/2 of the original volume, cooling, adjusting pH, adding an alcohol solution, stirring, performing still standing, and centrifuging by using a tube centrifuge; (5) membrane filtration: at 25-35 bar pressure at room temperature, ultrafiltering by using an organic ultrafiltration membrane, and then at 0.5-1.5 MPa pressure at room temperature, nano-filtering by using a nanofiltration membrane; (6) concentrating; (7) drying. By using the 1-deoxynojirimycin preparing method, the loss of a solvent can be reduced to a large extent, the energy consumption can be reduced and the product yield can be increased; therefore, the 1-deoxynojirimycin preparing method is suitable for industrial production.

The invention relates to the use of a deoxynojirimycin derivative, or pharmaceutically acceptable salt thereof, for the preparation of a medicament for the treatment of insulin resistance, hyperpigmentation and/or inflammatory processes in the skin, a fungal disease, overweight and obesity, or a microbacterial infection.

The invention belongs to the field of natural product chemistry, and relates to a method for preparing 1-deoxynojirimycin by a membrane separation technology. The method comprises the following steps of collecting fresh mulberry leaves, drying, crushing, adding deionized water into the crushed mulberry leaves for soaking, adding the soak into a nanofiltration membrane system with filtration to carry out filtration, adding the filtrate into a reverse osmosis membrane to carry out concentration, adding ethyl acetate into the concentrated filtrate to carry out extraction, carrying out pressure-reducing concentration of an aqueous phase obtained by the previous step, and carrying out vacuum drying to obtain the desired product. The method for preparing 1-deoxynojirimycin by a membrane separation technology has the advantages of simple process, good operability, low energy consumption, less pollution and good applicability for industrialized production.

The invention belongs to the field of detection of quality of traditional Chinese preparations, and particularly relates to a method for detecting a pharmaceutical preparation for treating the xiaoke disease. The pharmaceutical preparation comprises the active pharmaceutical ingredients, by weight, 6000-8000 parts of silkworm feces and 100-200 parts of radix glycyrrhizae. According to the method for detecting the pharmaceutical preparation, the pharmaceutical preparation is detected by measuring the content of 1-Deoxynojirimycin through an HPLC method. The method for detecting the pharmaceutical preparation is rapid, comprehensive and highly targeted, the quality of the preparation can be effectively controlled, and use safety and stability of the preparation can be improved.

The invention relates to the field of traditional Chinese medicine active site preparation, and discloses an active site of a mulberry twig, as well as a preparation method and application thereof. The method comprises the following steps: S1, after grinding the mulberry twig, heating, reflowing and extracting for 1-3 times at the temperature of 25-100 DEG C with 10-40 times of 25-95% ethyl alcohol, wherein the every-time extraction time is 60-300 min; filtering and concentrating an extracting solution to obtain a stock solution; S2, enriching and purifying the stock solution by strong-acid cation exchange resin and anion exchange resin in sequence to obtain the active site of the mulberry twig. The preparation method is simple and convenient to operate and good in repeatability, and the content of 1-Deoxynojirimycin (1-DNJ ) can be higher than 30.0%.

The invention discloses a preparation method of high-purity 1-deoxynojirimycin. 1-deoxynojirimycin is enriched by using ammonium cation exchange resin, and the 1-deoxynojirimycin is pulped and purified by combining anion exchange resin and a hot solvent to prepare the high-purity 1-deoxynojirimycin. The use of a high-concentration acid liquor can be avoided, so that equipment in a production workshop is prevented from being corroded by acids, the production risk is reduced, the energy consumption can be reduced, the solvent loss can be reduced, the product yield can be increased, and finally,the product with the mass content of 1-deoxynojirimycin being greater than 98.0% can be prepared.

The invention discloses a method for extracting 1-deoxynojirimycin from fresh mulberry leaves. The method comprises the following steps: cleaning and draining fresh picked mulberry leaves; cutting thedrained fresh mulberry leaves into strips, and putting the mulberry leaves into an extracting solution containing acetic acid; recovering an extracting solution after ultrasonic treatment; carrying out ultrasonic-assisted extraction on the mulberry leaves twice by taking purified water as an extraction solvent; combining extracting solutions, filtering, concentrating in vacuum, refrigerating, filtering, concentrating and drying to obtain an extract. The method does not need a large amount of organic solvents, and is simple in process, easy to operate and high in extraction rate. The obtainedextract can be used for preparing high-purity 1-deoxynojirimycin.

The invention discloses bacillus amyloliquefaciens capable of producing 1-deoxynojirimycin at high yield. The strain preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 19098. The invention also discloses a method for producing the 1-deoxynojirimycin by fermenting the bacillus amyloliquefaciens, and the yield of the 1-deoxynojirimycinreaches 0.9 g/L. The 1-deoxynojirimycin has alpha-glucosidase inhibitory activity and can be applied to blood glucose control of diabetics, so that the strain and the method for preparing the 1-deoxynojirimycin through fermentation of the strain have wide application prospects.

Disclosed are methods of preparing substituted nojirimycins represented by the following formula,

comprising reacting 1-deoxynojirimycin with a reagent prepared by reacting an oxidizing agent with the compound represented by the following formula.

The invention discloses an Auricularia auricula cultivation material rich in deoxynojirimycin. The cultivation material is prepared from, by weight, 3-5 parts of bean curd residues, 20-25 parts of ramulus mori, 1-2 parts of gypsum powder, 7-9 parts of edible fungus residues, 50-60 parts of peanut shells, 20-30 parts of pineapple peels and 10-16 parts of chelated emulsion. The ramulus mori is addedinto the cultivation material, mixed, stacked and fermented and blended with mulberry leaf extract; rich deoxynojirimycin is given to the cultivation material, so that the nutritional value of the finished product is improved; the raw material disclosed by the invention is scientific and reasonable in compatibility, comprehensive in formula nutrition, good in air permeability of fungus materialsand strong in water-retaining property, and can provide a good growth environment for the black fungus.

The invention provides a mulberry leaf extract rich in 1-deoxynojirimycin and a preparation method of the mulberry leaf extract. The preparation method comprises the following steps: S1, pretreatment: cleaning, drying and crushing mulberry leaves to obtain mulberry leaf powder; s2, ultrasonic treatment: adding the mulberry leaf powder into water, performing ultrasonic treatment and centrifugation, and taking supernate to obtain supernate 1 and residues; s3, performing enzymolysis treatment: adding water with the same mass as the residues, performing enzymolysis by using a compound enzyme 1, performing enzymolysis by using a compound enzyme 2, performing centrifugation, and taking supernate to obtain supernate 2; and S4, filtering, concentrating and drying: combining the supernatant 1 in the S2 and the supernatant 2 in the S3, and carrying out membrane filtration, membrane concentration and spray drying. The mulberry leaf extract obtained by the method is high in extraction rate, retains active ingredients in mulberry leaves to a great extent, has an extremely strong blood sugar reducing effect, is safe, good in quality and light in color, and has plant faint scent.

A stable pharmaceutical composition that includes an active agent selected from 1-deoxynojirimycin, a pharmaceutically acceptable salt thereof, or a derivative thereof, and a buffer, wherein the stable pharmaceutical composition is capable of being parenterally administered to a human without deleterious health effects. Pompe disease is an example of a lysosomal storage disorder. Pompe disease is caused by a deficiency in the enzyme acid alpha-glucosidase (GAA). GAA metabolizes glycogen, a storage form of sugar used for energy, into glucose.

The invention relates to a method for extracting ingredients of white mulberry and in particular relates to a method for extracting deoxynojirimycin from the white mulberry and purifying deoxynojirimycin. The method for extracting deoxynojirimycin from the white mulberry and purifying deoxynojirimycin comprises the following steps: carrying out water extraction, namely airing leaves of the white mulberry, then adding deionized water, boiling at constant temperature and extracting, filtering and collecting filtrate, adding the deionized water into filter residue and then extracting again, putting filtrate together, adding trichloroacetic acid and centrifuging, so that first supernate is obtained; loading onto a column, namely purifying the first supernate by adopting a reversed phase chromatography column, so that liquid containing deoxynojirimycin is obtained; carrying out ethanol precipitation and taking supernate, namely firstly adding ethanol with alcohol content of 55-65% into the liquid containing deoxynojirimycin obtained after the step of loading onto the column is finished, stirring for 3-8 minutes, then adding ethanol with alcohol content of 70-80%, and stirring for 3-5 minutes, so that second supernate is obtained; concentrating, namely concentrating the second supernate, so that concentrated liquor is obtained; and carrying out microwave vacuum drying, namely carrying out microwave vacuum drying on the concentrated liquor, so that a powder product containing deoxynojirimycin is obtained. The method for extracting deoxynojirimycin from the white mulberry and purifying deoxynojirimycin has the advantages of high extraction and purification efficiency and good separating effect.

The invention discloses a determination method of the content of 1-deoxynojirimycin in silkworm larvae. The determination method comprises the following steps of: taking 0.05mol/L HCl as a solvent and swirling for 15-20 seconds at a room temperature; extracting for two times by using a step of ultrasonic treatment for 20-30 minutes to effectively extract DNJ (Deoxynojirimycin) from the silkworm larvae; marking the DNJ for 20-25 minutes at 20 DEG C by utilizing 5mmol/L 9-fluorenyl methylclhlorofonmate (FMOC-C1) to form a DNJ-FMOC complex with the fluorescence; and detecting by utilizing a reverse phase high performance liquid chromatography provided with a fluorescence detector and calculating the content of the DNJ according to a peak area. The determination method of the content of the DNJ in the silkworm larvae provided with the invention has the advantages of high precision, repeatability and high sample recovery rate and good stability, can be used for detecting the content of the DNJ in a lot of silkworm larva products, and has active social benefits and economic benefits for propelling pharmaceutical development of the silkworm larvae.

The invention relates to a pharmaceutical composition, and in particular discloses a pharmaceutical composition for treating diabetes and complications thereof. The pharmaceutical composition consists of 1-deoxynojirimycin (1-DNJ for short) and resveratrol, wherein weight ratio of the 1-DNJ to the resveratrol is (1-5):15. The medicine disclosed by the invention can effectively inhibit alpha-glucosidase and reduce blood sugar concentration, and is more excellent in effect and lower in side effect in comparison with a simple blood sugar reducing compound; the pharmaceutical composition can be used for preparing blood sugar reducing medicines, food or healthcare products, as well as medicines, food or healthcare products for preventing or treating complications of diabetes.