8 results about "Deoxynojirimycine" patented technology

The invention belongs to the technical field of biology, and discloses a transaminase GabT1 mutant S106I, a construction method thereof and application of the transaminase GabT1 mutant S106I in biosynthesis of 1-deoxynojirimycin. According to the invention, the 106th serine near the molecular catalytic domain of the transaminase GabT1 derived from bacillus amyloliquefaciens is mutated into isoleucine in a site-directed mutagenesis manner, so that the catalytic efficiency of the transaminase GabT1 is remarkably improved, the problem that the catalytic efficiency of the transaminase GabT1 on fructose-6-phosphoric acid is not high at present is solved, and the application of the transaminase GabT1 to the fructose-6-phosphoric acid is promoted. The yield of 1-deoxynojirimycin synthesized by the mutant GabTS106I is increased by 43.87% compared with that of a wild type. The 1-deoxynojirimycin fermentation production capability of the transaminase GabT1 mutant and the engineering strain obtained by the invention is greatly improved, and the transaminase GabT1 mutant and the engineering strain have a good industrial application prospect.

The application provides an amphiphilic nobeliatin molecule, a preparation method thereof and a blood glucose lowering and antibacterial application to a diabetic wound. The application relates to the technical field of biomedical materials. The application synthesizes a novel amphiphilic nobeliatin molecule by taking 1-deoxynobeliatin as a hydrophilic part and an alkyl chain as a hydrophobic part. The compound can self-assemble into a cluster sugar molecule in an aqueous solution, has good blood glucose lowering activity through the "multivalent effect" of the cluster sugar-glycosidase, has good antibacterial activity through the tertiary amine structure of the azasugar molecule and the long alkyl chain and the bacterial membrane, and realizes the compatibility of the blood glucose lowering effect and the antibacterial effect. The synthesis raw material of the nobeliatin molecule provided by the application is rich in source, low in price, simple in preparation process and convenient for industrial application. Therefore, the discovery of the compound is expected to provide a promising treatment scheme for the development of blood glucose lowering and antibacterial integrated materials and the treatment of diabetic wounds, and has a wide potential application field.

A method for simultaneously preparing a chlorophyll paste and 1-deoxynojirimycin includes the following steps: (1) drying a silkworm excrement in a shaded area until the silkworm excrement reaches a specific moisture content; (2) placing the silkworm excrement into an extraction tank, adding an extraction solvent to obtain a mixture, and stirring the mixture with a water bath, filtering the mixture, the extraction solvent comprising both a non-polar solvent and a polar solvent; (3) subjecting the mixture to a coarse filtration to obtain a crude filtrate; (4) subjecting the crude filtrate to centrifugation, obtaining a chlorophyll-rich light phase and a 1-deoxynojirimycin-rich heavy phase; and (5) undergoing fine filtration and concentration with the chlorophyll-rich light phase to obtain a chlorophyll paste, and subjecting the 1-deoxynojirimycin-rich heavy phase with fine filtration, concentration, formulation, and spray drying to prepare 1-deoxynojirimycin.

Disclosed are a 1-deoxynojirimycin derivative and use thereof. According to the present invention, a variety of 1-deoxynojirimycin derivatives are prepared and obtained through screening. Compared with 1-deoxynojirimycin as a lead compound, the 1-deoxynojirimycin derivative can better bind to an amino acid site that is related to a target protein OPA1, stabilize a binding pocket at a dimer interface, promote the formation of an OPA1 dimer and repair a mitochondrial ultrastructure, thereby significantly saving mitochondrial functions and effectively improving a physiological state of cells. The 1-deoxynojirimycin derivative of the present invention can be used for preparing a drug for treating a disease that is related to unbalanced formation of the OPA1 dimer, and for example, can be used as a potential therapeutic drug for mitochondrial cardiomyopathy and other mitochondrial diseases.

This invention discloses a high-yield 1-deoxynojirimycin-producing *Bacillus pumilus* strain and its applications, belonging to the field of microbiology. This invention, through screening, ARTP and NTG mutagenesis, obtained a *Bacillus pumilus* strain with significantly increased 1-deoxynojirimycin production. Bacillus pumilus DNJ-BP01, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38218, was used to inoculate the seed culture of Bacillus pumilus DNJ-BP01 into a fermentation medium containing 5% beef extract supplemented with 2.5% mannitol as the carbon source and 3% potassium nitrate as the nitrogen source. After 76 hours of aerobic fermentation, the yield of 1-deoxynojirimycin reached 0.892 g / L. This invention provides a new fermentation strain for the efficient industrial production of 1-deoxynojirimycin and has broad application prospects.

The application discloses a method for detecting deoxy-najinmycin in milk and dairy products. The method comprises the following steps: extracting a sample solution to be derivatized by using an extracting agent, adding a buffer solution and a derivatization reagent to the sample solution to be derivatized to perform derivatization, performing liquid chromatography test on the sample to be tested after the derivatization, and qualitatively and quantitatively determining the sample according to the retention time and external standard method. The content of deoxy-najinmycin in the sample to be tested is obtained according to the test result. The extracting agent is an acetonitrile aqueous solution, the buffer solution is a potassium borate buffer solution, and the derivatization reagent comprises a fluorenylmethyloxycarbonyl chloride acetonitrile solution, a glycine solution and an acetic acid aqueous solution. The precision, repeatability, recovery rate and standard curve linearity of the detection method meet the industry requirements. The method has good extraction effect, high sensitivity, stable and accurate results, and is simple to operate and convenient to popularize.

The invention relates to the technical field of genetic engineering and microbiology, and discloses bacillus amyloliquefaciens for highly producing 1-deoxynojirimycin as well as a construction method and application of the bacillus amyloliquefaciens. The yield of 1-deoxynojirimycin (1-DNJ) in bacillus amyloliquefaciens is increased by knocking out the secondary thiamine phosphate synthase gene yjbQ. According to the bacillus amyloliquefaciens engineering bacterium LX-12 yjbQ, on the basis of plasmid T2 (2)-Ori, a knockout vector T2-delta yjbQ of a secondary thiamine phosphate synthase gene yjbQ is constructed, and the gene yjbQ is successfully knocked out from bacillus amyloliquefaciens LX-12, so that the bacillus amyloliquefaciens engineering bacterium LX-12 yjbQ is obtained. Compared with the bacillus amyloliquefaciens LX-12, the yield of the 1-deoxynojirimycin J of the engineering strain LX-12 yjbQ is at least increased by 26.17%, and the highest yield can reach 37.25%.

The application discloses a preparation method for obtaining 1-deoxynojirimycin from mulberry leaves, and mainly solves the problem that the prior art only considers product content and ignores yield, and comprises the following steps: (1) drying and crushing mulberry leaves, adding water to make pulp, adding vitamin C after adjusting pH, stirring uniformly, extracting, filtering and centrifuging to obtain a centrifugal liquid; (2) purifying the centrifugal liquid through two-stage OSN organic membrane to obtain a cut-off membrane liquid; (3) sequentially flowing the cut-off membrane liquid through more than five cation resin exchange columns in series, first eluting with 1-3 % ml / 100g of ammonia water, then eluting with 4-5.5 % ml / 100g of ammonia water, concentrating the eluate collected from the second column to the penultimate column to no ammonia water to obtain a extract; (4) loading the extract into a mixed ion resin exchange column, eluting with pure water, and collecting the eluate; (5) making the eluate pass through a reverse osmosis membrane to obtain a cut-off liquid, concentrating and vacuum drying. The method provided by the application reduces production processes and cost, improves product content and guarantees the recovery rate.