Method for extracting plant flavone compounds by enzymatic process

Abstract

The invention relates to a method for extracting plant flavone compounds by an enzymatic process, which comprises the following steps: (1) lixiviating, wherein enzymolysis water extraction is carried out once, and alcohol extraction is carried out twice; (2) purifying on the columns; (3) and processing the analytic solution to obtain the plant flavone compound product. Under the guidance of biotechnology theory, natural product chemical theory and technology and international drug test standard, the invention formulates an experimental scheme for production, and determines proper enzymatic process extraction technique and flavone glycoside biotransformation technique, thereby obtaining the product with high biological value. The invention determines to compound multiple enzyme preparations, optimizes the optimal transformation conditions and enhances the flavone biotransformation efficiency. The invention formulates a technical scheme of adsorption with macroporous absorbent resin to be used in industrial production, thereby increasing the content of aglycon flavones in the product.

Description

technical field

[0001] The invention belongs to the field of biotechnology, in particular to a method for enzymatically extracting plant flavonoids. Background technique

[0002] Plants are the most abundant natural resources available to human beings, and the flavonoid bioactive substances contained in plants are the main source of raw materials for food, chemical and pharmaceutical industries. However, these flavonoids are distributed in different parts of the plant in different glycoside forms, such as the fruits, leaves, and rhizomes of ginkgo, persimmon, walnut, hawthorn, Chinese toon, etc. These functional substances generally contain more aglycon-type flavonoids in plant fruits, but the proportion of low-activity flavonoid glycosides in plant stems and leaves is higher, but the content is higher than that in fruits. Therefore, it is of great theoretical and practical significance to enrich flavonoid bioactive substances in plant stems and leaves, modify them by chemi...

Examples

Embodiment 1

[0025] Embodiment 1 is example with Ginkgo biloba.

[0026] A method for enzymatically extracting plant flavonoids, the steps are as follows:

[0027] (1) Leaching

[0028] Ginkgo leaves are crushed into 20 mesh ginkgo leaf powder, 450kg of ginkgo leaf powder is weighed and packed into an extraction tank, and a solvent is added for extraction. Enzymatic water extraction once, alcohol extraction twice. Among them, the ginkgo leaves are collected from the dried ginkgo leaves in Ji County, Tianjin.

[0029] The first pass: enzymatic water extraction: add 2700L of water, 0.1% compound enzyme, pectinase: cellulase=7:3 (mass ratio) in the compound enzyme, keep the temperature at 50°C, extract for 2h, filter The pectinase and cellulase used in the present embodiment are the commercially available products of Tianjin Nuoao Technology Co., Ltd.

[0030] Second pass: add 2250L 80% ethanol solvent, extract at 70°C, extract for 2 hours, and filter;

[0031] The third pass: add 2250L ...

Embodiment 2

[0042] Embodiment 2, taking seabuckthorn apricot leaf as an example.

[0043] (1) Leaching

[0044] The seabuckthorn apricot leaves required for the test were crushed into 20 mesh seabuckthorn leaf powder, 450kg of the seabuckthorn leaf powder was weighed and packed into an extraction tank, and a solvent was added for extraction. Extract three times:

[0045] The first pass: enzymatic water extraction: add 2700L of water, 0.2% compound enzyme, in the compound enzyme, pectinase: cellulase=6:4 (mass ratio), extraction temperature is 50°C, extract for 2h, filter;

[0046] The second pass: add 2700L 70% ethanol solvent, extract at 75°C, extract for 2 hours, and filter;

[0047] The third pass: add 2700L 70% ethanol solvent, extract at 75°C, extract for 1 hour, and filter;

[0048] Combine the first and second extracts, concentrate to a solution of about 2000L, place the precipitate, and centrifuge after 3 hours to obtain a supernatant. The third extraction was directly used for ...

technical field

[0001] The invention belongs to the field of biotechnology, in particular to a method for enzymatically extracting plant flavonoids. Background technique

[0002] Plants are the most abundant natural resources available to human beings, and the flavonoid bioactive substances contained in plants are the main source of raw materials for food, chemical and pharmaceutical industries. However, these flavonoids are distributed in different parts of the plant in different glycoside forms, such as the fruits, leaves, and rhizomes of ginkgo, persimmon, walnut, hawthorn, Chinese toon, etc. These functional substances generally contain more aglycon-type flavonoids in plant fruits, but the proportion of low-activity flavonoid glycosides in plant stems and leaves is higher, but the content is higher than that in fruits. Therefore, it is of great theoretical and practical significance to enrich flavonoid bioactive substances in plant stems and leaves, modify them by chemi...

Examples

Embodiment 1

[0025] Embodiment 1 is example with Ginkgo biloba.

[0026] A method for enzymatically extracting plant flavonoids, the steps are as follows:

[0027] (1) Leaching

[0028] Ginkgo leaves are crushed into 20 mesh ginkgo leaf powder, 450kg of ginkgo leaf powder is weighed and packed into an extraction tank, and a solvent is added for extraction. Enzymatic water extraction once, alcohol extraction twice. Among them, the ginkgo leaves are collected from the dried ginkgo leaves in Ji County, Tianjin.

[0029] The first pass: enzymatic water extraction: add 2700L of water, 0.1% compound enzyme, pectinase: cellulase=7:3 (mass ratio) in the compound enzyme, keep the temperature at 50°C, extract for 2h, filter The pectinase and cellulase used in the present embodiment are the commercially available products of Tianjin Nuoao Technology Co., Ltd.

[0030] Second pass: add 2250L 80% ethanol solvent, extract at 70°C, extract for 2 hours, and filter;

[0031] The third pass: add 2250L ...

Embodiment 2

[0042] Embodiment 2, taking seabuckthorn apricot leaf as an example.

[0043] (1) Leaching

[0044] The seabuckthorn apricot leaves required for the test were crushed into 20 mesh seabuckthorn leaf powder, 450kg of the seabuckthorn leaf powder was weighed and packed into an extraction tank, and a solvent was added for extraction. Extract three times:

[0045] The first pass: enzymatic water extraction: add 2700L of water, 0.2% compound enzyme, in the compound enzyme, pectinase: cellulase=6:4 (mass ratio), extraction temperature is 50°C, extract for 2h, filter;

[0046] The second pass: add 2700L 70% ethanol solvent, extract at 75°C, extract for 2 hours, and filter;

[0047] The third pass: add 2700L 70% ethanol solvent, extract at 75°C, extract for 1 hour, and filter;

[0048] Combine the first and second extracts, concentrate to a solution of about 2000L, place the precipitate, and centrifuge after 3 hours to obtain a supernatant. The third extraction was directly used for ...