36results about How to "Improve biotransformation efficiency" patented technology

The invention relates to a method for extracting plant flavone compounds by an enzymatic process, which comprises the following steps: (1) lixiviating, wherein enzymolysis water extraction is carried out once, and alcohol extraction is carried out twice; (2) purifying on the columns; (3) and processing the analytic solution to obtain the plant flavone compound product. Under the guidance of biotechnology theory, natural product chemical theory and technology and international drug test standard, the invention formulates an experimental scheme for production, and determines proper enzymatic process extraction technique and flavone glycoside biotransformation technique, thereby obtaining the product with high biological value. The invention determines to compound multiple enzyme preparations, optimizes the optimal transformation conditions and enhances the flavone biotransformation efficiency. The invention formulates a technical scheme of adsorption with macroporous absorbent resin to be used in industrial production, thereby increasing the content of aglycon flavones in the product.

The invention provides a method for preparing arctigenin, which includes the steps: arctiin is used as a substrate, Beta-glucosidase enzyme liquid produced by the fermentation of aspergillus niger CGMCC No. 2594 is used as a catalyzer, the subsrate and the catalyzer are transformed in a shaker at the temperature of 20 DEG C to 50 DEG C and at the speed of 100r/min to 200r/min for 40h to 64h; and the transformed solution is separated and purified to obtain the arctigenin. The method has the advantages of: 1. mild reaction conditions and being environmental-friendly; 2. simple preparation process of a biocatalyst, namely the Beta-glucosidase enzyme liquid, needing no complex zymoprotein separation and purification as well as low cost; 3. simple operation and relatively high biological transformation efficiency; and 4. easy amplification of the biological transformation process and easy realization of large scale industrialized production. By utilizing the method for producing, the molar yield of the arctigenin can reach 94.7 percent and the purity of the product can reach 99.7 percent.

The invention relates to a method for preparing a growth promoter for edible fungi and application thereof. The method comprises the following steps of: dissolving, complexing, drying, mixing, and smashing the following components of raidx astragali leaching liquor, alginic acid, EDTA-Na, chitosan, fulvic acid, gibberellin, FeSO4, Zn SO4, LaSO4, CeSO4, KH2PO4, borax, Na2Mo2O4, ammonium polyphosphate, vitamins of B family and the like to obtain a mixture. The mixture has the advantages of rapid promotion of the edible fungi growth, enlargement of the fruiting bodies, accelerated growth, high performance of degrading and utilizing nutrients, improvement on stress resistance and disease resistance, yield increase, quality improvement, and nutrient value enhancement after the mixture is sprayed on fruiting bodies of the edible fungi.

The invention aims at providing a culturing system capable of improving fermentation efficiency of anaerobic gas feeding microorganisms. According to the system, a culturing medium is optimized, and therefore in the shake cultivation or continuous gas passing stir cultivation process, the gas and liquid mass transfer efficiency is improved, so that the fermentation efficiency of the anaerobic gas feeding microorganisms is improved. According to a method for improving the fermentation efficiency of the anaerobic gas feeding microorganisms, simethicone and/or kieselguhr are/is added into the culture medium and/or fermentation liquor of the anaerobic gas feeding microorganisms. According to the method, the gas and liquid mass transfer speed can be improved in the gas fermentation process, and the efficiency of gas biotransformation is effectively improved.

The invention belongs to the technical field of treating heavy metal pollution with microorganisms, discloses application of acidithiobacillus caldus and particularly discloses application of a new bacterial strain DX-csu in the acidithiobacillus caldus. A preservation number of the bacterial strain is CGMCC No. 14008 and the bacterial strain is named acidithiobacillus caldus. The acidithiobacillus caldus disclosed by the invention has good biotransformation action to iron and manganese-bond cadmium in soil and provides technical guidance for efficient metal leaching with the microorganisms, environmental treatment and the like.

The invention discloses a method for constructing a methanol quick utilization strain. The method comprises the steps of firstly, constructing a methanol and xylose mandatory co-utilization basic strain, then performing adaptability evolution on the basic strain by using methanol and xylose as carbon sources to obtain a strain of which the growth rate is significantly accelerated, and finally strengthening the activity of downstream metabolic pathway relevant enzymes of ribulose 5 phosphate in the strain. The invention further discloses the constructed methanol bioconversion strain and a method for performing methanol bioconversion through the strain. Through the strain disclosed by the invention, the methanol and the xylose can be used as the carbon sources, even the methanol is used as the only carbon source, methanol bioconversion is performed, and the conversion efficiency of the methanol is significantly improved. The invention further discloses mutants of some enzymes in the strain, and a method for performing methanol bioconversion through the mutants.

The invention discloses an amino acid polypeptide water-soluble fertilizer and a preparation method thereof. The water-soluble fertilizer is formed by carrying out microbial fermentation and proteasehydrolysis on feathers, and contains rich components such as amino acids, polypeptide proteins, organic acids, polyamines, minerals and the like. The preparation method comprises the following main steps: culturing anaerobic keratin degrading bacterium 18D-TA to obtain a fermentation seed solution; mixing feathers with the fermentation seed solution according to a mass/volume ratio of 1:10-1:1; carrying out culturing at a temperature of 55 DEG C for 16-24 hours; preserving heat at a temperature of 85 DEG C for 2-6 hours; and collecting the fermentation liquid to prepare the amino acid polypeptide water-soluble fertilizer. The amino acid polypeptide water-soluble fertilizer provided by the invention is simple in preparation method and mild in fermentation condition, and the water-soluble fertilizer with high content of polypeptide proteins and amino acids can be obtained. The amino acid polypeptide water-soluble fertilizer provided by the invention has good fertilizer efficiency and haseffects of promoting germination of plant seed, development of roots, growth of plants, flowering and fruiting, and the growth promoting effect is remarkable.

The invention belongs to the technical field of using microbes to treat heavy metal pollution, and discloses a novel strain (DX-csu) of Acidithiobacillus caldus, and a culture medium and a culture method thereof. The preservation number of Acidithiobacillus caldus DX-csu is CGMCC No.14008. The culture process has the advantages of low equipment requirements and simple and convenient operation. Thestrain has the advantages of quick growth and strong acid producing performance. The strain can well biologically convert cadmium that is combined with iron and manganese in soil and can be applied to high efficient metal leaching performed by microbes and environmental governance.

The invention relates to a preparation method for d-pseudoephedrine by a microbe transformation, belonging to the fermentation and biotransformation field, which is characterized in that a 1-phenyl-2-methylamino acetone is acted as a transformation precursor, the Morgan bacteria is transformed into a microbe, and the d-pseudoephedrine is produced through the procedures of fostering the microbe, collecting the cells, immobilizing the cells, and biotransformation. The preparation method for d-pseudoephedrine by a microbe transformation has the advantages of directly utilizing the microbe transformation to produce d-pseudoephedrine without a chemical resolving agent and a chemical splitting, not only saving the cost but also facilitating to protect the environment; having high efficiency of the biotransformation and strong specific target with the produced d-pseudoephedrine percentage being larger than 99.0%; possessing of simple process and low production cost because the other ephedrine is also able to produce based on the same process and equipment, for example, 1-pseudoephedrine.

The invention discloses a method for constructing a bacterial strain of which the methyl alcohol tolerance is improved through mutation of a cgl2365 gene and/ or a cgl2857 gene in the bacterial strain. The invention also discloses a constructed methyl alcohol biotransformation bacterial strain of which the methyl alcohol tolerance is improved, and a method for carrying out methyl alcohol biotransformation by the bacterial strain. When the bacterial strain constructed by the method disclosed by the invention utilizes a mixed carbon source formed by the methyl alcohol and other auxiliary carbon sources, the use ratio of the methyl alcohol is obviously improved, and therefore, the use efficiency of the bacterial strain for the methyl alcohol is substantially improved.

The invention discloses a method for constructing a bacterial strain of which the methyl alcohol tolerance is improved through weakening expression of a cgl0833 gene in the bacterial strain. The invention also discloses a constructed methyl alcohol biotransformation bacterial strain of which the methyl alcohol tolerance is improved, and a method for carrying out methyl alcohol biotransformation by the bacterial strain. While the bacterial strain constructed by the invention utilizes a mixed carbon source formed by the methyl alcohol and other auxiliary carbon sources, the use ratio of the methyl alcohol is obviously improved, and therefore, the use efficiency of the bacterial strain for the methyl alcohol is substantially improved.