36results about How to "Improve biotransformation efficiency" patented technology

The invention relates to a method for extracting plant flavone compounds by an enzymatic process, which comprises the following steps: (1) lixiviating, wherein enzymolysis water extraction is carried out once, and alcohol extraction is carried out twice; (2) purifying on the columns; (3) and processing the analytic solution to obtain the plant flavone compound product. Under the guidance of biotechnology theory, natural product chemical theory and technology and international drug test standard, the invention formulates an experimental scheme for production, and determines proper enzymatic process extraction technique and flavone glycoside biotransformation technique, thereby obtaining the product with high biological value. The invention determines to compound multiple enzyme preparations, optimizes the optimal transformation conditions and enhances the flavone biotransformation efficiency. The invention formulates a technical scheme of adsorption with macroporous absorbent resin to be used in industrial production, thereby increasing the content of aglycon flavones in the product.

The invention provides a method for preparing arctigenin, which includes the steps: arctiin is used as a substrate, Beta-glucosidase enzyme liquid produced by the fermentation of aspergillus niger CGMCC No. 2594 is used as a catalyzer, the subsrate and the catalyzer are transformed in a shaker at the temperature of 20 DEG C to 50 DEG C and at the speed of 100r / min to 200r / min for 40h to 64h; and the transformed solution is separated and purified to obtain the arctigenin. The method has the advantages of: 1. mild reaction conditions and being environmental-friendly; 2. simple preparation process of a biocatalyst, namely the Beta-glucosidase enzyme liquid, needing no complex zymoprotein separation and purification as well as low cost; 3. simple operation and relatively high biological transformation efficiency; and 4. easy amplification of the biological transformation process and easy realization of large scale industrialized production. By utilizing the method for producing, the molar yield of the arctigenin can reach 94.7 percent and the purity of the product can reach 99.7 percent.

The invention relates to a method for preparing a growth promoter for edible fungi and application thereof. The method comprises the following steps of: dissolving, complexing, drying, mixing, and smashing the following components of raidx astragali leaching liquor, alginic acid, EDTA-Na, chitosan, fulvic acid, gibberellin, FeSO4, Zn SO4, LaSO4, CeSO4, KH2PO4, borax, Na2Mo2O4, ammonium polyphosphate, vitamins of B family and the like to obtain a mixture. The mixture has the advantages of rapid promotion of the edible fungi growth, enlargement of the fruiting bodies, accelerated growth, high performance of degrading and utilizing nutrients, improvement on stress resistance and disease resistance, yield increase, quality improvement, and nutrient value enhancement after the mixture is sprayed on fruiting bodies of the edible fungi.

The invention aims at providing a culturing system capable of improving fermentation efficiency of anaerobic gas feeding microorganisms. According to the system, a culturing medium is optimized, and therefore in the shake cultivation or continuous gas passing stir cultivation process, the gas and liquid mass transfer efficiency is improved, so that the fermentation efficiency of the anaerobic gas feeding microorganisms is improved. According to a method for improving the fermentation efficiency of the anaerobic gas feeding microorganisms, simethicone and / or kieselguhr are / is added into the culture medium and / or fermentation liquor of the anaerobic gas feeding microorganisms. According to the method, the gas and liquid mass transfer speed can be improved in the gas fermentation process, and the efficiency of gas biotransformation is effectively improved.

The invention discloses a method for producing lactic acid from bagasse. The method comprises the following steps: grinding and filtering bagasse: adding water to the bagasse, carrying out wet grinding on the bagasse by virtue of a disc mill and sieving the bagasse; carryin gout green liquor-ethanol coupling pretreatment: carrying out a pretreatment course in a pressure-proof reaction kettle, adding the ground bagasse to the reactor and adding a green liquor, wherein heating temperature is 140-145 DEG C and reaction duration is 2-4h; filtering after the pretreatment so as to obtain solid residues; carrying out synchronous saccharification and fermentation: adding cellulase and lactobacillus strain to the solid residues obtained from pretreatment in a mode of taking the solid residues as a fermentation substrate, and reacting at 45 DEG C at pH value of 4.8-5.0 for 72-96h so as to produce the lactic acid. The method for producing the lactic acid from bagasse disclosed by the invention is high in conversion rate of hydrolyzing cellulase into cellulose and semi-cellulose; and the utilization rate of bagasse resource component is high and production cost is low.

The invention relates to the field of biological medicine, relates to a preparation process of bear gall powder, and in particular relates to a preparation process of artificial bear gall powder. According to the scheme, a conversion solution containing cell active substances is used, so that the technical problems that the purification step of enzyme is complex, the cost is high and the enzyme activity is not easy to maintain are solved. According to the preparation process, after bioconversion is performed on livestock gall powder, the ratio of tauroursodeoxycholic acid to taurochenodeoxycholic acid of the obtained artificial bear gall powder is similar to that of natural bear gall powder. The artificial bear gall powder in the scheme can be used as a substitute of the natural bear gallpowder for deep processing and further application. The artificial bear gall powder product produced by adopting the preparation process is small in batch difference, the production process can be standardized, the conversion efficiency is improved, and industrial large-scale production can be achieved.

The invention relates to a method for fruiting bag-cultivated mushroom at one time. The method comprises the operation steps of mushroom selection, inoculated culture, test tube expanding, cultivated species inoculation, cultivated species culture, strain treatment, raw material pretreatment, material batching and stirring, mushroom stick packaging, mushroom stick sterilization, mushroom stick inoculation, mushroom stick cultivation, mushroom stick coloring, mushroom stick pretreatment and mushroom stick fruiting. Coarse and fine culture base materials of a mushroom stick produced by utilizing the scheme are reasonably matched, nutrient substances are full, and perlite in a formula has the functions of holding moisture, ventilating air, regulating the potential of hydrogen and providing multiple microelements needed by the growth of the mushroom; meanwhile, oak chips and apple chips are mixed with corncobs, so that the ventilation of the mushroom stick is improved, the biotransformation rate of the mushroom is improved favorably, biotransformation of the mushroom stick can be promoted by virtue of a great number of microelements in canal mud, the uniformity of mushroom stick fruiting is improved by pre-cooling a mushroom bag, the mushroom stick fruiting speed is increased by utilizing the stimulation of the difference between pre-cooling temperature and external environment temperature, and the biotransformation rate is improved by 10-20 percent.

The invention discloses a microbial complex microbial inoculant for degrading kitchen garbage and a degrading method thereof, and particularly relates to the technical field of kitchen garbage treatment, the microbial complex microbial inoculant comprises complex thalli and solid auxiliary materials, and is characterized in that the complex thalli comprise cocci, bacillus and saccharomycetes, the cocci comprise nitrosococci and pediococcus pentosaceus, the bacillus comprises bacillus and saccharomycetes, and the saccharomycetes comprise bacillus and saccharomycetes. The bacillus comprises bacillus amyloliquefaciens, bacillus subtilis and amine bacillus, and the saccharomycetes comprise beer yeast and monascus purpureus. Water in the kitchen garbage is drained off, survival and reproduction of degrading bacteria are facilitated, the degradation efficiency is improved, meanwhile, the bacillus amyloliquefaciens can produce amylase, the bacillus subtilis can produce protease, and the prepared microbial complex microbial inoculant is used for fermentation of the kitchen garbage and can effectively promote decomposition of organic substances, so that the biotransformation efficiency is improved, the weight of the treated kitchen garbage is reduced by more than 80%, and the purposes of reduction, recycling and harmlessness of the kitchen garbage can be effectively achieved through the microbial complex microbial inoculant.

The invention belongs to the technical field of treating heavy metal pollution with microorganisms, discloses application of acidithiobacillus caldus and particularly discloses application of a new bacterial strain DX-csu in the acidithiobacillus caldus. A preservation number of the bacterial strain is CGMCC No. 14008 and the bacterial strain is named acidithiobacillus caldus. The acidithiobacillus caldus disclosed by the invention has good biotransformation action to iron and manganese-bond cadmium in soil and provides technical guidance for efficient metal leaching with the microorganisms, environmental treatment and the like.

The invention discloses a method for constructing a methanol quick utilization strain. The method comprises the steps of firstly, constructing a methanol and xylose mandatory co-utilization basic strain, then performing adaptability evolution on the basic strain by using methanol and xylose as carbon sources to obtain a strain of which the growth rate is significantly accelerated, and finally strengthening the activity of downstream metabolic pathway relevant enzymes of ribulose 5 phosphate in the strain. The invention further discloses the constructed methanol bioconversion strain and a method for performing methanol bioconversion through the strain. Through the strain disclosed by the invention, the methanol and the xylose can be used as the carbon sources, even the methanol is used as the only carbon source, methanol bioconversion is performed, and the conversion efficiency of the methanol is significantly improved. The invention further discloses mutants of some enzymes in the strain, and a method for performing methanol bioconversion through the mutants.

The invention discloses an amino acid polypeptide water-soluble fertilizer and a preparation method thereof. The water-soluble fertilizer is formed by carrying out microbial fermentation and proteasehydrolysis on feathers, and contains rich components such as amino acids, polypeptide proteins, organic acids, polyamines, minerals and the like. The preparation method comprises the following main steps: culturing anaerobic keratin degrading bacterium 18D-TA to obtain a fermentation seed solution; mixing feathers with the fermentation seed solution according to a mass / volume ratio of 1:10-1:1; carrying out culturing at a temperature of 55 DEG C for 16-24 hours; preserving heat at a temperature of 85 DEG C for 2-6 hours; and collecting the fermentation liquid to prepare the amino acid polypeptide water-soluble fertilizer. The amino acid polypeptide water-soluble fertilizer provided by the invention is simple in preparation method and mild in fermentation condition, and the water-soluble fertilizer with high content of polypeptide proteins and amino acids can be obtained. The amino acid polypeptide water-soluble fertilizer provided by the invention has good fertilizer efficiency and haseffects of promoting germination of plant seed, development of roots, growth of plants, flowering and fruiting, and the growth promoting effect is remarkable.

The invention belongs to the technical field of citrus processing, and relates to a preparation method of a citrus lactobacillus capsule with high flavone utilization degree, the method comprises the following steps: (1) raw material treatment: crushing a dried citrus peel raw material, adding soaked rice, stirring uniformly, steaming in a waterproof manner, and cooling; (2) adding koji for saccharification, namely adding sweet wine koji into the citrus peel raw material cooled to the saccharification temperature while the citrus peel raw material is hot, uniformly stirring, and saccharifying at constant temperature; (3) heating for enzyme deactivation: after saccharification is finished, heating the citrus peel saccharified substance to 65 DEG C or above, and cooling after enzyme deactivation; (4) adding lactobacillus: adding the activated lactobacillus in the RMS liquid culture medium into the orange peel saccharified substance cooled to the room temperature, uniformly stirring, and performing semi-solid constant-temperature fermentation in a sealed state; (5) squeezing and separating; and (6) post-treatment. According to the preparation method of the citrus lactobacillus capsule with high flavone availability, provided by the invention, the bioavailability of a citrus flavone product is improved, and meanwhile, an intestinal health-care effect is taken into account.

The invention belongs to the technical field of using microbes to treat heavy metal pollution, and discloses a novel strain (DX-csu) of Acidithiobacillus caldus, and a culture medium and a culture method thereof. The preservation number of Acidithiobacillus caldus DX-csu is CGMCC No.14008. The culture process has the advantages of low equipment requirements and simple and convenient operation. Thestrain has the advantages of quick growth and strong acid producing performance. The strain can well biologically convert cadmium that is combined with iron and manganese in soil and can be applied to high efficient metal leaching performed by microbes and environmental governance.

The invention relates to a preparation method for d-pseudoephedrine by a microbe transformation, belonging to the fermentation and biotransformation field, which is characterized in that a 1-phenyl-2-methylamino acetone is acted as a transformation precursor, the Morgan bacteria is transformed into a microbe, and the d-pseudoephedrine is produced through the procedures of fostering the microbe, collecting the cells, immobilizing the cells, and biotransformation. The preparation method for d-pseudoephedrine by a microbe transformation has the advantages of directly utilizing the microbe transformation to produce d-pseudoephedrine without a chemical resolving agent and a chemical splitting, not only saving the cost but also facilitating to protect the environment; having high efficiency of the biotransformation and strong specific target with the produced d-pseudoephedrine percentage being larger than 99.0%; possessing of simple process and low production cost because the other ephedrine is also able to produce based on the same process and equipment, for example, 1-pseudoephedrine.

The invention discloses a method for constructing coexistence of aerobic fungi and facultative or anaerobic microorganisms by utilizing 3D printing, which comprises the following steps: inoculating the aerobic fungi into a fermentation culture medium, and placing a supporting material with pores on the fermentation culture medium, so that the aerobic fungi form a compact biological membrane on the supporting material; facultative or anaerobic microorganisms are made into a living body material of a fence structure through 3D printing; and putting the living body material into a fermentation culture medium, coexisting with aerobic fungi, and carrying out fermentation reaction. The invention also provides a bioreactor constructed by using the 3D printed container and the living body material. According to the invention, oxygen consumption is realized through biological membranes formed by aerobic fungi on a support material and a living body material, proper growth and production conditions are created for facultative or anaerobic microorganisms in the living body material, and a bioreactor is designed through the living body material and 3D printing equipment; the oxygen gradient is generated in the bioreactor by utilizing the oxygen consumption of aerobic bacteria, and the oxygen requirement of chemical production based on a CBP system is met.

The invention discloses a method for constructing a bacterial strain of which the methyl alcohol tolerance is improved through mutation of a cgl2365 gene and / or a cgl2857 gene in the bacterial strain. The invention also discloses a constructed methyl alcohol biotransformation bacterial strain of which the methyl alcohol tolerance is improved, and a method for carrying out methyl alcohol biotransformation by the bacterial strain. When the bacterial strain constructed by the method disclosed by the invention utilizes a mixed carbon source formed by the methyl alcohol and other auxiliary carbon sources, the use ratio of the methyl alcohol is obviously improved, and therefore, the use efficiency of the bacterial strain for the methyl alcohol is substantially improved.

The invention discloses an application of a novel microbial strain Saccharomyces cerevisiae CGMCC No.2230 in microbial transformation preparation of (S)-(-)-3-chlorine-1-phenylpropanols. The application of the invention is that: 3-chlorine-1-propiophenones are taken as substrates, and immobilized cell particles which are prepared by fermentation broth obtained after fermentation of the Saccharomyces cerevisiae strain CGMCC No.2230 are taken as biocatalysts; transformation reaction time of transformation liquid is 8 to 72 hours at the temperature of 25 to 30 DEG C in dibutyl phthalates, and the product S)-(-)-3-chlorine-1-phenylpropanols are obtained after separation and purification of the transformation liquid. The microbial transformation method has the advantages of friendly surroundings, mild reaction conditions, simple product separation, high substrate transformation ratio, good product purity and biocatalysts capable of being reused, and is suitable for commercial process.

The invention relates to a novel cytochrome P450 gene, expressed protein and application thereof, which belong to the filed of biotechnology. The P450 gene encodes CYP107213 enzyme and has the nucleotide sequence shown as SEQ ID NO 1. The P450 gene has the function of oxidizing abamectin 4'-hydroxyl into 4'-carbonyl specifically in sites, provides a novel functional gene resource for biologicallyconverting abamectin, thereby laying the foundation of further reformation and recombination of the gene, improvement of biological conversion efficiency of abamectin and reduction of production costof methylamino abamectin benzoate. Wild Streptomyces sp.ZB01 containing the gene has the function of oxidizing abamectin 4'-hydroxyl into 4'-carbonyl specifically in sites with the conversion efficiency of 36.8% which is obviously greater than that of current wild Streptomyces and that of most ema heterologous expressed proteins.

The invention discloses a method for constructing a bacterial strain of which the methyl alcohol tolerance is improved through weakening expression of a cgl0833 gene in the bacterial strain. The invention also discloses a constructed methyl alcohol biotransformation bacterial strain of which the methyl alcohol tolerance is improved, and a method for carrying out methyl alcohol biotransformation by the bacterial strain. While the bacterial strain constructed by the invention utilizes a mixed carbon source formed by the methyl alcohol and other auxiliary carbon sources, the use ratio of the methyl alcohol is obviously improved, and therefore, the use efficiency of the bacterial strain for the methyl alcohol is substantially improved.

The invention discloses a preparation method of fermented lentinula edodes submicron powder. The method comprises the following steps of baking lentinula edodes, and crushing the baked lentinula edodes into lentinula edodes powder of 20-30 meshes; fermenting the lentinula edodes powder for the first time with yeast prepared from radish seeds, glossy ganoderma, toadstool and poria cocos; then adding lentinula edodes powder, and performing fermentation twice with inonotus cuticularis; draining lentinula edodes liquid after fermentation twice into lentinula edodes fermented submicron powder; andfinally, performing baking, and performing packaging to obtain finished products of the lentinula edodes submicron powder. According to the preparation method disclosed by the invention, the nutrientvalue, the health-care efficacy and the absorptivity of the lentinula edodes are increased, so that the nutrients and the functional components of the lentinula edodes are fully exerted, and nutrientsare reserved; and fibers are tiny, so that the mouth feel is better, and the quality of the lentinula edodes micro powder is significantly improved.

The invention relates to a preparation method for d-pseudoephedrine by a microbe transformation, belonging to the fermentation and biotransformation field, which is characterized in that a 1-phenyl-2-methylamino acetone is acted as a transformation precursor, the Morgan bacteria is transformed into a microbe, and the d-pseudoephedrine is produced through the procedures of fostering the microbe, collecting the cells, immobilizing the cells, and biotransformation. The preparation method for d-pseudoephedrine by a microbe transformation has the advantages of directly utilizing the microbe transformation to produce d-pseudoephedrine without a chemical resolving agent and a chemical splitting, not only saving the cost but also facilitating to protect the environment; having high efficiency of the biotransformation and strong specific target with the produced d-pseudoephedrine percentage being larger than 99.0%; possessing of simple process and low production cost because the other ephedrineis also able to produce based on the same process and equipment, for example, 1-pseudoephedrine.

The invention relates to a method for fruiting bag-cultivated mushroom at one time. The method comprises the operation steps of mushroom selection, inoculated culture, test tube expanding, cultivated species inoculation, cultivated species culture, strain treatment, raw material pretreatment, material batching and stirring, mushroom stick packaging, mushroom stick sterilization, mushroom stick inoculation, mushroom stick cultivation, mushroom stick coloring, mushroom stick pretreatment and mushroom stick fruiting. Coarse and fine culture base materials of a mushroom stick produced by utilizing the scheme are reasonably matched, nutrient substances are full, and perlite in a formula has the functions of holding moisture, ventilating air, regulating the potential of hydrogen and providing multiple microelements needed by the growth of the mushroom; meanwhile, oak chips and apple chips are mixed with corncobs, so that the ventilation of the mushroom stick is improved, the biotransformation rate of the mushroom is improved favorably, biotransformation of the mushroom stick can be promoted by virtue of a great number of microelements in canal mud, the uniformity of mushroom stick fruiting is improved by pre-cooling a mushroom bag, the mushroom stick fruiting speed is increased by utilizing the stimulation of the difference between pre-cooling temperature and external environment temperature, and the biotransformation rate is improved by 10-20 percent.

The invention discloses a magnetically immobilized yeast cell and its application in the preparation of (R)-2-hydroxy-4-phenylbutyrate ethyl ester. The magnetically immobilized yeast cell is made of lysine-modified magnetic nano The particles are mixed with the yeast cell solution, fixed in a shaker at 20°C-40°C, 130-170r / min for 2-6h, after the reaction, magnetically separated, the precipitate is washed with deionized water, and freeze-dried to obtain magnetically immobilized yeast cell. The immobilization method of the invention effectively improves the stability of the yeast cells and enhances the tolerance of the cells to the substrate solution. Under the action of an external magnetic field, the magnetically immobilized cells are easier to separate from the reaction system, which is beneficial to the separation and extraction of products and simplifies the process flow. Appropriate magnetic field frequency and magnetic field strength in the alternating magnetic field reactor can effectively enhance the mass transfer process of the reaction system, significantly shorten the reduction reaction time, and then improve the biotransformation efficiency.

The invention discloses a high-ammonia-nitrogen autotrophic denitrification device, which belongs to the technical field of wastewater treatment. The device includes a reactor main body, the bottom of which is connected to a third pump body through a delivery pipe body, and a three-phase separator, which is arranged in the upper part of the reactor main body , the bottom side wall of the reactor main body is connected with the second pump body through the conveying pipe body, the filler filling assembly is arranged in the reactor main body, and the ultrasonic processing assembly is arranged above the three-phase separator, and the ultrasonic processing assembly includes a hollow fiber membrane, a hollow fiber There is a superoxide combination pipe body above the membrane, and the superoxide combination pipe body is connected to the superoxide assembly respectively. The superoxide assembly is composed of a low-frequency ultrasonic vibrator and an air pump. The loss of the medium shortens the initial start-up time, realizes the microcirculation flow of the internal medium, improves the mass transfer effect, and ensures the stable operation of the denitrification process.

The invention belongs to the technical field of microbial control of heavy metal pollution, and discloses the application of a thermophilic acid thiobacillus, specifically the application of a new strain DX-csu in Acidithiobacillus caldus. The preservation number of the strain is CGMCC No.14008; naming For Acidithiobacillus caldus. The Acidithiobacillus caldus bacterium of the invention has a good biotransformation effect on the cadmium in the iron-manganese bonded state in the soil, and provides technical guidance for efficient metal leaching by microorganisms, environmental treatment and the like.

The present invention discloses a method for increasing the alcohol fermentation yield through a Trichoderma viride fermentation extract. The method at least comprises: (1) adopting straws as a raw material to carry out solid state fermentation, wherein straw powder is added to a fermentation barrel, water and ammonium nitrate are added, complete stirring is performed, Trichoderma viride CGMCC3.6619 is inoculated to make the strain and the straw powder completely contact, continuous culturing is performed for one month, and drying is performed after completing the fermentation to obtain a fermentation matrix for spare; (2) carrying out extraction and purification on the extract alcohol fermentation auxiliary agent, wherein the fermentation matrix is soaked with a NaOH solution, constant temperature treatment and filtration are performed, the filtrate is concentrated to prepare a raw liquid, the pH value of the raw liquid is adjusted to 2.0, standing, centrifugation and precipitate removing are performed, and the supernatant is subjected to spray drying to a product alcohol fermentation auxiliary agent; and (3) carrying out alcohol fermentation, starch is taken and added to clear water, stirring is performed to make the starch be uniformly dispersed in the water, liquid high-temperature amylase and calcium chloride are added, the pH value is adjusted, liquid glucoamylase is added, cooling is performed after completing the saccharification, activated yeast and the alcohol fermentation auxiliary agent are added, and fermentation is performed in a constant temperature chamber.