Method for improving fermentation efficiency of anaerobic gas feeding microorganisms

A technology for microbial fermentation and microorganisms, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as low efficiency, and achieve the effect of improving efficiency and gas-liquid mass transfer rate.

Active Publication Date: 2015-04-22
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the current microbial gas fermentation has the problem of low efficiency

Method used

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  • Method for improving fermentation efficiency of anaerobic gas feeding microorganisms
  • Method for improving fermentation efficiency of anaerobic gas feeding microorganisms
  • Method for improving fermentation efficiency of anaerobic gas feeding microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Shaking culture at constant temperature: add 2ml mineral solution stock, 0.05g yeast extract and 0.5g morpholine ethanesulfonic acid, and a certain proportion of diatomaceous earth and / or simethicone to 100ml system, 5ml reducing agent stock , filled with 99.999% nitrogen at a rate of 2 L / min for 5 minutes, sealed, and sterilized at 121 degrees for 20 minutes. After sterilization, cool to about 40 degrees, and then add 0.1ml of trace element storage solution to complete the construction of the culture system. Insert 10ml of Clostridium carboxidivorans bacterial strain P7 or Clostridium ljungdahlii bacterial strain DSM 13528 culture solution cultivated to the logarithmic growth phase, then continuously feed simulated syngas (gas composition: 50% CO, 35% CO 2 , 15%H 2 ) to balance for 5 minutes, pressurize to 0.6bar to seal. Shake culture at 37 degrees and 180 rpm, and take samples at regular intervals for fermentation product detection.

[0025] The constructed cultur...

Embodiment 2

[0030] Embodiment 2: continuous aeration and stirring culture

[0031] In the continuous aerated culture system of Clostridium carboxidivorans strain P7 with added substances, the production of acetic acid was significantly higher than that of the culture system composed of the basic medium, the basic medium system, the basic medium system + 5% silicone oil system and the basic medium system + The content of acetic acid in the 5% diatomite system has reached 9.8g / L, 28g / L and 19g / L ( Figure 5 ). Compared with the basal culture system, the yield of the latter two systems increased by 185% and 93.9% respectively. Compared with the shaking culture system, the continuous aeration and stirring culture method can give full play to the advantages of this culture system.

Embodiment 3

[0032] Example 3: Effects of Different Proportions of Additives on the Alcohol Production Effect of Fermentation System

[0033] 1%, 2%, 3%, 4% and 5% simethicone oil were added to the basal medium of Clostridium carboxidivorans strain P7 for fermentation, and the effect of butanol fermentation was detected. It is found that different proportions of additives will increase the butanol production of the system to varying degrees, and the butanol production of the fermentation system with the additive concentration from low to high is 70mg / L, 82mg / L, 97mg / L, 131mg / L , 157mg / L and 168mg / L ( Image 6 ), with the increase of the addition ratio, the butanol yield also showed a significant increase.

[0034]Clostridium ljungdahlii strain DSM 13528 was added 1%, 2%, 3%, 4% and 5% diatomaceous earth to the basal medium for fermentation, and the ethanol fermentation effect was detected. It is found that different proportions of additives will increase the ethanol production of the sys...

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Abstract

The invention aims at providing a culturing system capable of improving fermentation efficiency of anaerobic gas feeding microorganisms. According to the system, a culturing medium is optimized, and therefore in the shake cultivation or continuous gas passing stir cultivation process, the gas and liquid mass transfer efficiency is improved, so that the fermentation efficiency of the anaerobic gas feeding microorganisms is improved. According to a method for improving the fermentation efficiency of the anaerobic gas feeding microorganisms, simethicone and/or kieselguhr are/is added into the culture medium and/or fermentation liquor of the anaerobic gas feeding microorganisms. According to the method, the gas and liquid mass transfer speed can be improved in the gas fermentation process, and the efficiency of gas biotransformation is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of microbial gas fermentation, and in particular relates to a method for improving the fermentation efficiency of anaerobic gas microorganisms. technical background [0002] With the rapid development of my country's economy, power plants, steel plants and other industrial and mining enterprises discharge a large amount of industrial tail gas into the atmosphere, which not only causes serious environmental pollution, but also causes a series of environmental problems, and also causes serious harm to human health. This type of industrial tail gas is rich in CO, CO 2 and H 2 , is a kind of cheap raw material for the manufacture of petrochemical chemicals with great development value. How to fully develop and utilize these industrial tail gases can not only reduce environmental pressure, but also form substitute products for petrochemical resources, so as to alleviate my country's growing demand for petroleum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12P7/08C12R1/145
CPCC12P7/06C12P7/16Y02E50/10
Inventor 黄志勇韩一凡王兴彪王敬敬张小霞郭亚琼
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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