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Application of a Strain of Thermophilic Thiobacillus

A technology of rod-shaped bacteria and strains, which is applied in the application field of new strains of Thiobacillus thermophilicum, can solve problems such as excessive heavy metal content and endogenous soil pollution, and achieve strong acid production capacity, high biotransformation efficiency, and growth quick effect

Active Publication Date: 2020-04-24
CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption and accumulation of heavy metals in the soil gradually lead to the heavy metal content in the soil seriously exceeding the standard, and the soil endogenous pollution is serious

Method used

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  • Application of a Strain of Thermophilic Thiobacillus
  • Application of a Strain of Thermophilic Thiobacillus
  • Application of a Strain of Thermophilic Thiobacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the isolation of thermophilic acid sulfur bacillus (Acidithiobacillus caldusDX-csu)

[0030] Isolation of Acidithiobacillus caldusDX-csu: Collect 10g of ore sample from the ore in Dexing, add it to a conical flask containing 100ml of sterile water and glass beads, shake it on a shaker at 170r / min for 20min, and take 1ml Add 9ml sterile water, dilute 10 successively -5 , 10 -6 , 10 -7 , 10 -8 , 10 -9 , 10 -10 times, take 1ml of the above suspension and add it to 100ml sterilized 9K medium, repeat each concentration 3 times, place it in a 45°C incubator for 0.5d, 1d and 3d, repeat the above steps 4-5 times for bacteria for separation and purification.

Embodiment 2

[0031] Embodiment 2, identification of thermophilic acid sulfur bacillus (Acidithiobacillus caldusDX-csu)

[0032] (1) The sequencing results of the 16SrRNA gene sequence of Acidithiobacillus caldus DX-csu strain showed that the 16SrRNA gene sequence of Acidithiobacillus caldus DX-csu strain was shown in the sequence table, and the homology comparison was carried out with the 16SrRNA gene sequence of Thermophilic acidsulfobacillus in Genbank according to the sequencing results , the results showed that Acidithiobacillus caldus DX-csu belongs to thermophilic acid sulfur bacillus.

[0033] (2) The sequence results of the whole genome gene sequence of Acidithiobacillus caldusDX-csu strain showed that the sequence results of the whole gene of Acidithiobacillus caldusDX-csu strain were homologously compared with the whole gene sequence of Acidithiobacillus caldusDX- csu is a new strain.

Embodiment 3

[0034] Embodiment 3, the physiological property of thermophilic acid sulfur bacillus (Acidithiobacillus caldus)

[0035] Four different kinds of thermophilic acid sulfur bacillus (Acidithiobacillus caldus) culture medium solution pH and Eh of four strains that this laboratory screened were compared and Eh changes with time; Its result shows that thermophilic acid sulfur bacillus (Acidithiobacillus caldusDX-csu) of the present invention ) pH is significantly lower than the other three, and the results show that the Eh of Acidithiobacillus caldusDX-csu of the present invention is significantly higher than the other three, indicating that the strain of the present invention has strong acid production ability. See attached figure 1 ,2.

[0036] Compare four strains of different kinds of thermophilic acid sulfur bacillus (Acidithiobacillus caldus) culture medium bacterium concentration change with time; Its result shows that thermophilic acid sulfur bacillus (Acidithiobacillus cal...

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Abstract

The invention belongs to the technical field of microbial control of heavy metal pollution, and discloses the application of a thermophilic acid thiobacillus, specifically the application of a new strain DX-csu in Acidithiobacillus caldus. The preservation number of the strain is CGMCC No.14008; naming For Acidithiobacillus caldus. The Acidithiobacillus caldus bacterium of the invention has a good biotransformation effect on the cadmium in the iron-manganese bonded state in the soil, and provides technical guidance for efficient metal leaching by microorganisms, environmental treatment and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial control of heavy metal pollution, and in particular relates to the application of a new strain of thermophilic sulfur bacillus that grows rapidly and has high biotransformation efficiency for soil cadmium. Background technique [0002] Soil is an important part of the ecological environment. In various environmental elements, such as areas with serious water pollution and air pollution, along with natural phenomena such as water flow, rainfall, and atmospheric flow, pollutants will eventually enter the soil and gradually transform into soil pollution. make the soil the ultimate receptor for pollutants. The adsorption and accumulation of heavy metals in the soil gradually lead to the heavy metal content in the soil seriously exceeding the standard, and the soil endogenous pollution is serious. At present, the adverse effects caused by soil pollution have attracted much attention and become a hot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/20C12N1/205C12R2001/01
Inventor 刘宏伟柏连阳蒋慧丹刘学端尹华群梁伊丽胡瑾
Owner CENT SOUTH UNIV
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