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Strain of Acidithiobacillus caldus and culture medium and culture method thereof

A culture method and technology of bacillus, applied in the field of new strain of Thiobacillus thermophilicum and its culture medium, can solve the problems of soil endogenous pollution, excessive heavy metal content, etc., and achieve high biotransformation efficiency and high yield Strong acid ability, rapid growth effect

Active Publication Date: 2018-02-16
CENT SOUTH UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption and accumulation of heavy metals in the soil gradually lead to the heavy metal content in the soil seriously exceeding the standard, and the soil endogenous pollution is serious

Method used

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  • Strain of Acidithiobacillus caldus and culture medium and culture method thereof
  • Strain of Acidithiobacillus caldus and culture medium and culture method thereof
  • Strain of Acidithiobacillus caldus and culture medium and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the isolation of thermophilic acid sulfur bacillus (Acidithiobacillus caldus DX-csu)

[0028] Isolation of Acidithiobacillus caldus DX-csu Collect 10g of ore sample from the ore in Dexing, add it to a triangular flask with 100ml sterile water and glass beads inside, vibrate on a shaker at 170r / min for 20min, take 1ml and add 9ml of sterile water, successively dilute 10 -5 , 10 -6 , 10 -7 , 10 -8 , 10 -9 , 10 -10 Take 1ml of the above suspension and add it into 100ml sterilized 9K medium, repeat each concentration 3 times, place it in a 45°C incubator for 0.5d, 1d and 3d, repeat the above steps 4-5 times for bacteria for separation and purification.

Embodiment 2

[0029] Embodiment 2, identification of thermophilic acid sulfur bacillus (Acidithiobacillus caldus DX-csu)

[0030] (1) The sequencing results of the 16SrRNA gene sequence of the Acidithiobacilluscaldus DX-csu strain showed that the 16SrRNA gene sequence of the Acidithiobacilluscaldus DX-csu strain was shown in the sequence table. It indicated that Acidithiobacillus caldus DX-csu belonged to thermophilic acid sulfur bacillus.

[0031] (2) The results of the whole genome gene sequence sequencing of the Acidithiobacillus caldus DX-csu strain showed that the sequence results of the whole gene of the Acidithiobacillus caldus DX-csu strain were homologously compared with the whole gene sequence of the thermophilic acid sulfur bacillus in Genbank, and the results showed that Acidithiobacillus caldus DX-csu is a new strains.

Embodiment 3

[0032] Embodiment 3, the physiological property of thermophilic acid sulfur bacillus (Acidithiobacillus caldus)

[0033]Four different kinds of thermophilic acid sulfur bacillus (Acidithiobacillus caldus) culture medium solution pH and Eh of four different strains screened in this laboratory have been compared; The pH is obviously lower than the other three, and the results show that the Eh of the present invention is obviously higher than that of the other three, indicating that the strain of the present invention has strong acid production ability. See attached figure 1 ,2.

[0034] Have compared bacterium concentration change with time in the thermophilic acid sulfur bacillus (Acidithiobacillus caldus) substratum of four different kinds; three kinds. See attached image 3 .

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Abstract

The invention belongs to the technical field of using microbes to treat heavy metal pollution, and discloses a novel strain (DX-csu) of Acidithiobacillus caldus, and a culture medium and a culture method thereof. The preservation number of Acidithiobacillus caldus DX-csu is CGMCC No.14008. The culture process has the advantages of low equipment requirements and simple and convenient operation. Thestrain has the advantages of quick growth and strong acid producing performance. The strain can well biologically convert cadmium that is combined with iron and manganese in soil and can be applied to high efficient metal leaching performed by microbes and environmental governance.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of heavy metal pollution, and specifically relates to a new strain of thermophilic sulfur bacillus that grows rapidly and has high biotransformation efficiency for soil cadmium, as well as its culture medium and cultivation method. Background technique [0002] Soil is an important part of the ecological environment. In various environmental elements, such as areas with serious water pollution and air pollution, along with natural phenomena such as water flow, rainfall, and atmospheric flow, pollutants will eventually enter the soil and gradually transform into soil pollution. make the soil the ultimate receptor for pollutants. The adsorption and accumulation of heavy metals in the soil gradually lead to the heavy metal content in the soil seriously exceeding the standard, and the soil endogenous pollution is serious. At present, the adverse effects caused by soil pollution have attra...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 刘宏伟柏连阳蒋慧丹刘学端梁伊丽尹华群胡瑾
Owner CENT SOUTH UNIV
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