A fruity selenium-enriched Cordyceps militaris yogurt product and preparation method thereof

A technology of Cordyceps militaris and dairy products, which is applied in the direction of milk preparations, biochemical equipment and methods, dairy products, etc., can solve the problems of not being able to meet the needs of health, low selenium content, etc., and achieve the promotion of absorption, good taste, and increased absorption The effect of the ability of inorganic selenium

Active Publication Date: 2020-11-13
JINLIN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of selenium in natural foods is generally low, and it is far from enough to meet the needs of health by relying on selenium intake from food to maintain life and exercise.

Method used

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  • A fruity selenium-enriched Cordyceps militaris yogurt product and preparation method thereof
  • A fruity selenium-enriched Cordyceps militaris yogurt product and preparation method thereof
  • A fruity selenium-enriched Cordyceps militaris yogurt product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of Cordyceps militaris protoplasts: according to mycelium mass (g): enzyme solution volume (mL) = 1:1, add sterile mixed enzyme solution, and place in a shaker at 26°C for 2.5 hours of enzymatic hydrolysis . Centrifuge the enzymolysis solution at 6000 r / min for 12 min, discard the supernatant, wash the precipitate with the osmotic agent for 3 times, centrifuge to thoroughly remove the residual enzyme solution, add the osmotic agent for oscillation, and centrifuge at 2500 r / min for 12 min. Take the supernatant and centrifuge at 6000 r / min for 12 min, the protoplasts sink to the bottom of the centrifuge tube, add 2 parts of osmotic stabilizer to shake and suspend;

[0026] (2) Selenium-enriched culture of Cordyceps militaris: adding Na to the prepared Cordyceps militaris biomass 2 SeO 3 60 μg / L, after standing at 26°C for 8 hours, transfer 5% into 100mL Cordyceps militaris regeneration medium, and culture at 26°C with shaking (150 r / min) for 4 days;

[...

Embodiment 2

[0032] (1) Preparation of Cordyceps militaris protoplasts: according to mycelium mass (g): enzyme solution volume (mL) = 1:1, add sterile mixed enzyme solution, and place in a shaker at 26°C for 2.5 hours of enzymatic hydrolysis . Centrifuge the enzymolysis solution at 6000 r / min for 12 min, discard the supernatant, wash the precipitate with the osmotic agent for 3 times, centrifuge to thoroughly remove the residual enzyme solution, add the osmotic agent for oscillation, and centrifuge at 2500 r / min for 12 min. Take the supernatant and centrifuge at 6000 r / min for 12 minutes, the protoplasts sink to the bottom of the centrifuge tube, add 4 parts of osmotic stabilizer to shake and suspend;

[0033](2) Selenium-enriched culture of Cordyceps militaris: adding Na to the prepared Cordyceps militaris biomass 2 SeO 3 60 μg / L, after standing at 26°C for 8 hours, transfer 5% into 100mL Cordyceps militaris regeneration medium, and culture at 26°C with shaking (150 r / min) for 4 days; ...

Embodiment 3

[0039] (1) Preparation of Cordyceps militaris protoplasts: according to mycelium mass (g): enzyme solution volume (mL) = 1:1, add sterile mixed enzyme solution, and place in a shaker at 26°C for 2.5 hours of enzymatic hydrolysis . Centrifuge the enzymolysis solution at 6000 r / min for 12 min, discard the supernatant, wash the precipitate with the osmotic agent for 3 times, centrifuge to thoroughly remove the residual enzyme solution, add the osmotic agent for oscillation, and centrifuge at 2500 r / min for 12 min. Take the supernatant and centrifuge at 6000 r / min for 12 min, the protoplasts sink to the bottom of the centrifuge tube, add 6 parts of osmotic stabilizer to shake and suspend;

[0040] (2) Selenium-enriched culture of Cordyceps militaris: adding Na to the prepared Cordyceps militaris biomass 2 SeO 3 60 μg / L, after standing at 26°C for 8 hours, transfer 5% into 100mL Cordyceps militaris regeneration medium, and culture at 26°C with shaking (150 r / min) for 4 days;

[...

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Abstract

The invention discloses a fruity selenium-enriched Cordyceps militaris yogurt product and a preparation method thereof. By preparing the Cordyceps militaris biomass, the cell wall permeability of the Cordyceps militaris strain is changed, and the selenium-enriched culture is carried out at the same time, which can significantly improve the selenium-enriched content of the Cordyceps militaris ability, and then use low-fat and low-sugar skim milk, selenium-enriched Cordyceps militaris fermented mycelia homogenate as raw materials, and add hawthorn powder, pine pollen, vitamin C, etc. to carry out mixed-bacteria fermented yogurt to prepare a low-fat and low-sugar , the selenium-enriched Cordyceps militaris yogurt with the function of lowering blood lipids solves the problems of single function of fresh yogurt, is not conducive to storage, and cannot be eaten by diabetics, and enhances the health care function of lowering blood lipids of yogurt. As a health food, it has a good development prospect.

Description

technical field [0001] The invention discloses a fruit-flavored selenium-enriched Cordyceps militaris yogurt product and a preparation method thereof. The selenium-enriched Cordyceps militaris mycelium and defatted fresh milk whose cell wall permeability is changed and the selenium-enriching ability is obviously enhanced are used as main raw materials, and Add hawthorn powder, pine pollen solution, sweetener and vitamins and other auxiliary materials, inoculate Streptococcus thermophilus, Lactobacillus bulgaricus, Lactococcus lactis subsp. diacetyl, and Lactobacillus acidophilus for fermentation to obtain solid yogurt The invention belongs to the technical field of fermented yogurt. Background technique [0002] Yogurt is made by fermenting milk, which has better nutritional value and absorption efficiency than raw milk. Many studies have shown that yogurt has outstanding effects in various health and nutritional functions such as fighting cancer caused by diet and stress, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C9/123A23C9/13C12N1/14C12R1/645
Inventor 董媛王会岩蔡建辉李艳于欢郭健刘微王皓朱洁郝峰姜勇张宸豪
Owner JINLIN MEDICAL COLLEGE
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