A fruity selenium-enriched Cordyceps militaris yogurt product and preparation method thereof
A technology of Cordyceps militaris and dairy products, which is applied in the direction of milk preparations, biochemical equipment and methods, dairy products, etc., can solve the problems of not being able to meet the needs of health, low selenium content, etc., and achieve the promotion of absorption, good taste, and increased absorption The effect of the ability of inorganic selenium
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Embodiment 1
[0025] (1) Preparation of Cordyceps militaris protoplasts: according to mycelium mass (g): enzyme solution volume (mL) = 1:1, add sterile mixed enzyme solution, and place in a shaker at 26°C for 2.5 hours of enzymatic hydrolysis . Centrifuge the enzymolysis solution at 6000 r / min for 12 min, discard the supernatant, wash the precipitate with the osmotic agent for 3 times, centrifuge to thoroughly remove the residual enzyme solution, add the osmotic agent for oscillation, and centrifuge at 2500 r / min for 12 min. Take the supernatant and centrifuge at 6000 r / min for 12 min, the protoplasts sink to the bottom of the centrifuge tube, add 2 parts of osmotic stabilizer to shake and suspend;
[0026] (2) Selenium-enriched culture of Cordyceps militaris: adding Na to the prepared Cordyceps militaris biomass 2 SeO 3 60 μg / L, after standing at 26°C for 8 hours, transfer 5% into 100mL Cordyceps militaris regeneration medium, and culture at 26°C with shaking (150 r / min) for 4 days;
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Embodiment 2
[0032] (1) Preparation of Cordyceps militaris protoplasts: according to mycelium mass (g): enzyme solution volume (mL) = 1:1, add sterile mixed enzyme solution, and place in a shaker at 26°C for 2.5 hours of enzymatic hydrolysis . Centrifuge the enzymolysis solution at 6000 r / min for 12 min, discard the supernatant, wash the precipitate with the osmotic agent for 3 times, centrifuge to thoroughly remove the residual enzyme solution, add the osmotic agent for oscillation, and centrifuge at 2500 r / min for 12 min. Take the supernatant and centrifuge at 6000 r / min for 12 minutes, the protoplasts sink to the bottom of the centrifuge tube, add 4 parts of osmotic stabilizer to shake and suspend;
[0033](2) Selenium-enriched culture of Cordyceps militaris: adding Na to the prepared Cordyceps militaris biomass 2 SeO 3 60 μg / L, after standing at 26°C for 8 hours, transfer 5% into 100mL Cordyceps militaris regeneration medium, and culture at 26°C with shaking (150 r / min) for 4 days; ...
Embodiment 3
[0039] (1) Preparation of Cordyceps militaris protoplasts: according to mycelium mass (g): enzyme solution volume (mL) = 1:1, add sterile mixed enzyme solution, and place in a shaker at 26°C for 2.5 hours of enzymatic hydrolysis . Centrifuge the enzymolysis solution at 6000 r / min for 12 min, discard the supernatant, wash the precipitate with the osmotic agent for 3 times, centrifuge to thoroughly remove the residual enzyme solution, add the osmotic agent for oscillation, and centrifuge at 2500 r / min for 12 min. Take the supernatant and centrifuge at 6000 r / min for 12 min, the protoplasts sink to the bottom of the centrifuge tube, add 6 parts of osmotic stabilizer to shake and suspend;
[0040] (2) Selenium-enriched culture of Cordyceps militaris: adding Na to the prepared Cordyceps militaris biomass 2 SeO 3 60 μg / L, after standing at 26°C for 8 hours, transfer 5% into 100mL Cordyceps militaris regeneration medium, and culture at 26°C with shaking (150 r / min) for 4 days;
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