71 results about "Flavone glycosides" patented technology

The invention discloses a plant-source nutritional supplement based on glycoside compounds and a preparation method thereof, and relates to the technical field of preparation of plant-source nutritional supplements.The preparation method comprises the steps that raw materials are prepared; the preparation method comprises the following steps: respectively carrying out ultrasonic-assisted extraction on flavonoid glycoside, saponin and geniposide raw materials to obtain an extracting solution; carrying out centrifugal separation on the extracting solution, taking supernate, and then purifying by adopting a macroporous resin adsorption method to obtain a purified glycoside compound solution; mixing the purified glycoside compound solution with lycium barbarum polysaccharide, Chinese yam starch, vitamin E and other auxiliaries in proportion, and uniformly stirring to form a mixed solution; carrying out spray drying on the mixed solution to obtain a powdery nutritional supplement; and screening the powdery nutritional supplement. The high-purity plant source glycoside compound and the functional auxiliary agent are scientifically compounded, and ultrasonic extraction, resin purification and precise process control are combined, so that the integrated improvement of activity synergy, quality stability and personalized scene adaptation of the nutritional supplement is realized.

The invention discloses a flavonoid glycoside glycosyltransferase LbUGT71BX1 in laportea bulbifera as well as the coding gene and the use thereof. The amino acid sequence of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 2. The nucleotide sequence of the coding gene of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 1. According to the invention, on the basis of relevant results of the second-generation transcriptome and the third-generation full-length transcriptome sequencing of the laportea bulbifera, the last-step key enzyme LbUGT71BX1 for the synthesis of the flavonoid glycoside in the laportea bulbifera is screened and identified by using a reverse genetics method, filling the terminal blank of the biosynthesis pathway of the flavonoid glycoside in the laportea bulbifera.

The invention belongs to the technical field of sleep-aiding oral liquids, and discloses a sleep-aiding nerve-calming oral liquid and a preparation process thereof, the oral liquid is composed of the following components: a plant fermentation broth, fructo-oligosaccharide, gamma-aminobutyric acid, casein hydrolysis peptide, a saussurea involucrata culture, and pyrroloquinoline quinone disodium salt; wherein the plant fermentation liquor is prepared from spina date seeds, poria cocos, lilies, Chinese dates, lotus seeds, lophatherum gracile, seville orange flowers, red roses with double petals, roselle, wheat, licorice roots, peach kernels, sweet-scented osmanthus and plant lactobacillus BXM2. The core component spina date seed saponin A is combined with gamma-aminobutyric acid to directly regulate the GABAergic nervous system, shorten the sleep latency and effectively help sleep and tranquilize the nerves; casein hydrolysis peptide promotes secretion of melatonin, and cooperates with the saussurea involucrata culture to resist oxidative stress, improve the sleep structure and restore circadian rhythm; small molecular active peptides and flavonoid glycosides are generated through fermentation, and anxiety mood, fatigue state and qi and blood circulation are improved; nano embedding and targeted delivery technologies are adopted in the whole process, the bioavailability is high, and the risk of drug dependence is avoided.

The invention relates to the technical field of fine processing of agricultural products, and provides a method and a system for quantitatively distinguishing peels of soft-branch stock oranges and processing type oranges, and the method comprises the following steps: S1 to S4, selecting two types of mature oranges, freeze-drying and grinding peels, and mixing the peels according to a preset proportion to obtain peel residues; weighing the same amount of the sample, adding methanol, and carrying out ultrasonic extraction, centrifugation and filtration by a filter membrane to obtain a to-be-detected solution; and calculating the proportion of the polyoxymethylene flavone glycoside in the soft-branch rootstock orange peel through HPLC (High Performance Liquid Chromatography) detection by combining an integral peak area of a standard curve of the polyoxymethylene flavone glycoside. The invention further discloses a system for executing the method. According to the method, the polyoxymethylene flavone glycoside is used as an index, HPLC detection and standard curve calculation are combined, whether the fruit peel is contained or not can be clearly distinguished, the proportion can be accurately quantified, an objective traceable basis is provided, the cost is low, the popularization rate is high, the sample treatment process is applicable, and a safe and reliable scheme is provided for high-value utilization and quality control of by-products in the citrus industry.

The invention relates to the technical field of functional beverages, in particular to a composite polypeptide solid beverage with a uric acid reducing function and a preparation method of the composite polypeptide solid beverage. The composite polypeptide solid beverage with the uric acid reducing function is prepared from the following raw materials in percentage by weight: 15-25% of plukenetia volubilis extract, 30-40% of lysimachia christinae hance-plantain herb composite extract, 8-12% of oyster peptide powder, 5-8% of earthworm protein peptide freeze-dried powder and 3-5% of a flavoring agent. Wherein the ratio of a lysimachia christinae hance extract to a plantain herb extract in the lysimachia christinae hance-plantain herb composite extract is 3: The flavoring agent is erythritol and / or stevioside, xanthine oxidase is inhibited by using flavonoid substances, GLUT9 expression is synergistically down-regulated by using bispyribaloside, PRPP synthetase activity is regulated by using zinc-rich peptide, uric acid micro-crystals are dissolved by using earthworm protein peptide, a multi-target action network of flavonoid-glycoside-bioactive peptide is achieved, and the effect of improving the bioavailability of the flavonoid-glycoside-bioactive peptide is achieved. And triple mechanisms of excretion promotion, generation inhibition and kidney protection are realized.

The present invention discloses a golden chrysanthemum flavonoid glycoside derived from golden chrysanthemum, wherein the golden chrysanthemum flavonoid glycoside is apigenin 7-O-(6"-O-malonyl)-β-D-glucoside derived from golden chrysanthemum. The present invention separates and purifies apigenin 7-O-(6"-O-malonyl)-β-D-glucoside from golden chrysanthemum for the first time, completes the first synthesis through a 5-step synthesis process with a total yield of 2.7%, and discovers for the first time that the compound has activity in improving hyperlipidemia and fatty liver in vitro and in vivo. Therefore, the compound can be used in the preparation of products for treating related diseases such as hyperlipidemia and fatty liver.

The present invention relates to an extract containing Myconoside, which is derived from an in vitro system of a plant of the family Gesneraceae, in particular from the genus Haberlea and Ramonda, including species Haberlea rhodopsis Friv., Ramonda helichii (Bois.C. B. Clarke, Ramonda myconi (L.) Rchb., Ramonda nathaliae Pancicamp, and a method for preparing the extract containing Myconoside. The extract containing Myconoside, which is derived from an in vitro system of a plant of the family Gesneraceae, in particular from the genus Haberlea and Ramonda, includes the species of Haberlea rhodopsis Friv. The invention relates to an extract, in particular to petrovic, Ramonda serbic Panic, Petrovic and Ramonda serbic Panic, as well as hybrids thereof, and a preparation method of the extract. Furthermore, the invention relates to the use of said extract as a chemical, radiation and UV protection agent in human and veterinary medicine, in nutritional supplements and in cosmetics. The plant in-vitro system extract is used independently or in a mixed mode, and comprises a polyphenol component extract 1 mainly containing flavone-C glycoside and phenethyl alcohol compounds (PHE) and a Mycoside component extract 2 mainly containing phenethyl alcohol compounds (particularly Mycoside and Paucifloside). The extract 1 contains 0.1-55.0 wt% of PHE, free phenol, saccharides, organic acid, fatty acid, amino acid, sterol and the like, and the total amount is 100 wt%; the extract 2 contains 55.0 to 99.9 weight percent of PHE (particularly Myconoside and Paucifloside) and phenolic compounds, monosaccharides, disaccharides or residues thereof, and the total amount is 100 weight percent. These compounds, in particular Myconoside, have high biological activity, anti-oxidation, anti-mutation and anti-chromosome-disruption activity, and thus are suitable for use alone, also can be used in combination by metered mixing, or can be conditioned by dilution, for the preparation of natural chemical, radio and ultraviolet protective agents with a controllable degree of protection. The latter is used in humans and veterinary, nutritional supplements and cosmetics to prevent and treat harmful chemical, radiation and UV effects.

The invention discloses lotus leaf powder as well as a preparation method and application thereof, and belongs to the technical field of deep processing of plant raw materials. The preparation method of the lotus leaf powder provided by the invention comprises the following steps: carrying out enzymolysis on pretreated fresh lotus leaves in a system containing beta-glucosidase, and then carrying out scalding and ice water leaching cooling; the obtained lotus leaf powder has bright emerald green color, and contains more active ingredients such as flavonoid aglycones and nuciferine which are beneficial to human body absorption; moreover, the content of tannin and total flavonoid glycoside in the obtained lotus leaf powder is relatively low, so that the lotus leaf powder has relatively low bitter and astringent taste, and the obtained lotus leaf powder is excellent in mouth feel; meanwhile, the obtained lotus leaf powder also has good capability of improving blood fat; the method can be widely applied to preparation of matcha-flavored food or preparation of functional preparations for improving blood fat.

The invention provides albizia flower compound essential oil with sleep aiding and depression relieving functions and a preparation method of the albizia flower compound essential oil, and relates to the technical field of aromatherapy and natural product application. The compound essential oil takes an albizia flower low-temperature two-stage extract as a core carrier and is compounded with various functional essential oil components such as bitter orange leaf essential oil, FCF bergamot essential oil and Egypt nymphaea tetragona original essence, and the compound essential oil is prepared through three core innovations of albizia flower carrier synergistic technology, multi-channel nerve regulation design and safety strengthening design. The technical problems that existing sleep-aiding essential oil is single in effect and high in potential nerve irritation and the bioavailability of albizia flower flavonoid glycoside is low are solved. Experimental verification shows that the compound essential oil can shorten the sleep latency period by 52% and reduce the anxiety score by 47%, can cooperatively regulate the GABA energy system, 5-hydroxytryptamine secretion and hypothalamus-pituitary-adrenal axis through an olfaction-nerve reflex path, realizes efficient anxiety resistance, deep sleep promotion and emotion balance, and is suitable for people with various anxiety insomnia and emotional disorder.

The application provides application of a glycosyltransferase mutant in catalyzing synthesis of flavone glycoside compounds, and belongs to the technical field of biological enzymes.The application provides a mutant AAVT of a glycosyltransferase VcUGT1 screened from blueberries, wherein, compared with the glycosyltransferase VcUGT1, the mutant AAVT has point mutations of S367A, V274A, F82V and I132T.The mutant can significantly improve the catalytic activity of a glycosyltransferase reaction, and can efficiently catalyze substrates to generate corresponding 7-O-glucoside compounds.The enzyme catalysis reaction of the mutant can produce flavone-7-O-glucoside compounds with medical value in a large amount in industrialization, and the catalysis reaction condition is mild and green and environmentally friendly, and the mutant has the prospect of industrialization development and application.

The invention relates to and provides a fingerprint spectrum of musk-chuan NaoLiantong granules and a detection method for quantitative analysis of multiple components by single marker. The fingerprint spectrum detection method has the characteristics of simplicity and convenience in operation and good stability and reproducibility. 13 common peaks exist in an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of 15 batches of musk-chuan naoeutong granules, and 7 components in the fingerprint spectrum, namely geniposidic acid, chlorogenic acid, cowherb seed flavonoid glycoside, ferulic acid, senkyunolide I, salvianolic acid B and tanshinone IIA, are identified. The similarity of the 15 batches of the musk and chuan naoliantong granules is 0.961-1. According to the fingerprint spectrum detection method, 13 representative common characteristic peaks are determined so as to comprehensively reflect the internal quality characteristics of the musk-chuan naoliantong preparation.

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

The invention relates to the technical field of modern preparations of medicinal and edible medicinal diets, in particular to a lily and kumquat traditional Chinese medicine ointment special for pregnant women to relieve cough and moisten the lung and a preparation process thereof, the lily and kumquat traditional Chinese medicine ointment is prepared from kumquat, Lanzhou sweet lily, Xinhui pericarpium citri reticulatae and other components, and also contains a kumquat flavonoid glycoside-cyclodextrin inclusion compound and a lily polysaccharide-zinc complex; carrying out ultrasonic extraction on flavonoid glycoside from kumquats, then carrying out inclusion with cyclodextrin, complexing lily polysaccharide with zinc, and carrying out microencapsulation on the folium eriobotryae extract; the raw materials and Chinese yam water are mixed, decocted and concentrated, active ingredients are added for compounding, and crystallization is performed after homogenization and sterilization to obtain the ointment. The ointment improves the content and stability of effective components through inclusion, complexation and microencapsulation technologies, is high in 24-hour slow release rate, quick in effect taking, high in clinical effective rate, good in taste, free of additives and suitable for pregnant women, and achieves safe and effective cough relieving.

This invention relates to the field of plant tissue culture and the breeding of superior varieties of traditional Chinese medicinal materials. This invention discloses a method for rapid propagation of Epimedium inflorescences via tissue culture, comprising the following steps: S1, collecting newly sprouted flower inflorescences of Epimedium in early spring, disinfecting them, and setting them aside; S2, cutting the disinfected inflorescences into 1.1-1.2 cm segments, inoculating them into callus induction medium, and culturing them under conditions of 1200-1300 lux light intensity, 12 h / day photoperiod, and 24℃. This invention ensures uniform seedling quality and stable traits through targeted screening of effective components and monitoring of genetic stability. The optimized culture medium ratios at each stage result in a high proliferation coefficient, robust rooting, and a short propagation cycle, enabling efficient year-round propagation. Standardized seedling hardening and transplanting lead to a high survival rate. This method is technically stable and highly applicable, enabling the mass production of high-flavonoid glycoside, genetically stable, high-quality seedlings, fundamentally solving problems such as germplasm degradation, low propagation coefficient, and uneven quality, providing strong support for the breeding and industrialization of superior varieties of Epimedium.

The application belongs to the field of Chinese herbal medicines, and particularly relates to a method for preparing acylated flavonoid glycoside B (kaempferol-3-O-[6'''-O-E-p-coumaroyl-beta-D-glucosyl-(1->2)-alpha-L-rhamnose glycoside]) from ginkgo leaf extract. The method first uses macroporous adsorption resin to preliminarily purify the ginkgo leaf extract, and then uses activated white clay to decolorize the ginkgo leaf extract. After that, the decolorized flow fraction is separated by using flash medium-pressure preparative chromatography and silica gel column chromatography, and a large amount of acylated flavonoid glycoside B is successfully prepared. The purity of the separated acylated flavonoid glycoside B reaches UV >= 98%, ELSD >= 98%, and the recovery rate is high, wherein the acylated flavonoid glycoside B is 75%. The method has the advantages of large preparation amount, high stability, strong repeatability, simple process steps, high purity of the separated monomeric compound, and the like, and is an ideal separation process, which lays a foundation for subsequent industrial scale production.

The present invention relates to the field of feed additive technology, and in particular to a feed additive composition and application thereof. The present invention provides a feed additive composition, wherein the composition comprises ursolic acid and flavonoid glycosides in a mass ratio of (0.5-5): 1. The present invention finds that ursolic acid and flavonoid glycosides can play a synergistic effect in reducing the mortality rate of livestock and poultry. In the case of equal dietary addition, the effect of ursolic acid and flavonoid glycosides used in combination is significantly higher than that of ursolic acid and flavonoid glycosides added separately. The feed additive composition provided by the present invention can effectively reduce the mortality rate of livestock and poultry, improve the growth performance of livestock and poultry, promote their weight gain, increase average daily feed intake and average daily weight gain, reduce feed-to-weight ratio to a certain extent, and is of great significance for the development of feed additives for replacing antibiotics.

The present invention discloses a flavonoid glycoside combination for treating chronic obstructive pulmonary disease, comprising a tricholoma glycoside-irisin composition. The tricholoma glycoside-irisin composition is prepared with normal saline into a suspension at a concentration of 0.5 to 1.5 g / kg. Also provided are a method for preparing the flavonoid glycoside combination for treating chronic obstructive pulmonary disease and use of the flavonoid glycoside combination in preparing a medicament for treating chronic obstructive pulmonary disease. The flavonoid glycoside combination of the present invention is easy to obtain and has a good effect in treating chronic obstructive pulmonary disease.

The invention discloses a method for performing enzymolysis on flavonoid glycoside by using industrial saccharifying enzyme, and belongs to the technical field of bioactive substance conversion. The method comprises the following steps: dissolving a flavonoid glycoside substrate by using a DMSO solution to obtain a dissolved solution; then adding the dissolving solution, amyloglucosidase and a citrate buffer solution into a pore plate, putting the pore plate into a microplate reader, and carrying out enzymolysis efficiency test; and after the reaction is finished, wrapping the pore plate with tinfoil, and putting the pore plate into a freezing layer to inactivate the enzyme. The amyloglucosidase provided by the invention has a wider substrate spectrum, can realize comprehensive conversion of glycoside components with different structures in various flavone-rich complex plant raw materials, and does not need to independently adapt enzymes for specific glycoside types or raw material types; meanwhile, the amyloglucosidase can be used for treating various types of plant raw materials by virtue of a single enzyme seed, so that the use types of enzyme preparations are reduced, and the process complexity and cost are reduced.

The present application relates to the technical field of traditional Chinese medicine detection, and provides a genuine three-leafed knot thin-layer identification kit and method, comprising: a chrysophanol control solution, a neochlorogenic acid control solution and a rapid extraction solvent, the rapid extraction solvent contains a low-carbon alcohol, which is used for extracting flavonoid glycoside components in three-leafed knot stem and leaf samples, and the kit is used for identifying the origin of three-leafed knot by detecting the content of chrysophanol and the presence of neochlorogenic acid. The present application uses a chrysophanol and neochlorogenic acid double-index mutual exclusion identification logic, combined with a rapid extraction solvent system without drying, to realize on-site, rapid and accurate identification of the genuineness of three-leafed knot, and effectively solves the problem of origin confusion.

A method for decomposing flavonoid glycosides, comprising heating a raw material containing flavonoid glycosides in a sealed container in the presence of alcohol at a temperature exceeding the boiling point of the alcohol at normal pressure, thereby decomposing the flavonoid glycosides into flavonoids.

The invention provides a preparation method of a mixed molecularly imprinted polymer and a method for simultaneously extracting various flavonoid glycosides from a ginkgo biloba extract, and relates to the technical field of plant processing. According to the invention, rutin, isoquercitrin and apigenin-7-O-glucoside are used as template molecules to prepare a molecularly imprinted polymer (MIP); various characterizations prove that the preparation and synthesis are successful, and adsorption capacity tests prove that the compound has adsorption capacity on specific flavonoid glycoside. The MIP is added into the ginkgo flavone crude extract, and the total flavone content of the product after adsorption is increased from 24% to 90% or above. The four flavonoid glycoside compounds including rutin, isoquercitrin, 3-O rutinoside and apigenin-7-O-glucoside are obtained at a time, and it is indicated that the MIP has good adsorption performance, can be recycled and can be used for rapidly enriching the flavonoid glycoside compounds.

The invention belongs to the technical field of traditional Chinese medicine component detection and analysis, and particularly relates to a method for detecting various active components in lonicerae flos by quantitative analysis of multi-components by single marker. According to the QAMS detection method for the various active ingredients in the lonicera confusa, based on a simple, convenient and rapid high performance liquid chromatography technology, QAMS of the active ingredients such as flavonoids, flavonoid glycosides, iridoid glycosides and organic acids in the lonicera confusa can be achieved, and qualitative and quantitative detection of the various active ingredients is included. The detection target objects comprise 14 active components, namely neochlorogenic acid, cumaric acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, swertiamarin, secologanin, rutin, quercitrin, galuteolin, ferulic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C. The invention further provides a method for detecting the content of the isochlorogenic acid. Tests prove that the method disclosed by the invention meets methodological requirements, has the characteristics of simplicity and convenience in operation, rapidness in detection, high sensitivity, accuracy in determination and strong applicability, is beneficial to accurately evaluating the quality of the lonicerae flos and provides a reliable basis for safe application of the lonicerae flos.

The invention provides an anti-atherosclerosis lipidosome carrier drug and a preparation method thereof, and belongs to the technical field of biological pharmacy, the lipidosome carrier drug is prepared by taking tussah peptide, premna microphylla leaf flavonoid glycoside, clausena lansium leaf polysaccharide and troxerutin as active ingredients. The antheraea pernyi peptide is a product between 1kDa and 5kDa obtained by extracting protein from antheraea pernyi and sequentially carrying out enzymolysis by bacillus subtilis protease and pepsin. The premna microphylla leaf flavonoid glycoside is prepared by performing enzymolysis and water extraction on premna microphylla leaves and then purifying the premna microphylla leaves through an HPD-600 macroporous adsorption resin column. The clausena lansium leaf polysaccharide is prepared by the following steps: extracting clausena lansium leaves with water, precipitating with ethanol, redissolving, and purifying through a DEAE-52 cellulose column and a Sephadex G-100 gel column in sequence. All the components aim at key pathological links of atherosclerosis respectively, and the core advantages of high efficiency, safety and stability are achieved through multi-component synergistic compatibility and closed-loop optimization of a fine preparation process.

The invention discloses a flavonoid glycoside compound as well as a preparation method and application thereof. The invention provides a flavonoid glycoside compound 2 ''-O-benzoyl isoorientin. The compound is a new flavonoid glycoside compound which is separated from gentiana rigescens for the first time. The invention further provides a preparation method of the 2 ''-O-benzoyl isoorientin, and further provides application of the flavonoid glycoside compound 2 ''-O-benzoyl isoorientin in preparation of anti-aging cosmetics or drugs and application of the flavonoid glycoside compound 2 ''-O-benzoyl isoorientin in preparation of anti-tumor drugs.

The present invention discloses a flavonoid aglycone composition for treating chronic obstructive pulmonary disease. The flavonoid aglycone composition includes maackiain and tectorigenin, and the mass ratio of maackiain to tectorigenin is 7:1 to 3:2. The present invention also discloses the application of the flavonoid aglycone composition in the medicine for treating chronic obstructive pulmonary disease. The present invention combines maackiain in the genuine medicinal material Sophora flavescens and tectorigenin in Belamcanda chinensis, and has the effect of treating chronic obstructive pulmonary disease. The contents of these two flavonoid aglycones, maackiain and tectorigenin, in their medicinal materials are very high and are easily obtained. Therefore, developing them into drugs for treating COPD has great clinical and market value and is worthy of vigorous promotion and application.

The present application relates to the technical field of biotechnology, and proposes a composition for preventing and treating grape downy mildew, a preparation method and application thereof.The composition for preventing and treating grape downy mildew comprises cyazofamid and flavonoid glycoside extract, and the mass ratio of cyazofamid to flavonoid glycoside extract is 5:150-250.Through the above technical solution, the problem of poor prevention and treatment effect of the existing technology on grape downy mildew is solved.

The invention belongs to the technical field of plant puree preparation, and particularly relates to dendrobium puree as well as a preparation method and application thereof. According to the preparation method, firstly, a 1% sodium bicarbonate aqueous solution is used for conducting heat extraction on dendrobium nobile, the 1% sodium bicarbonate aqueous solution can neutralize the acid environment, and acidic hydrolysis of polysaccharide in the extraction process is inhibited; according to the method, an ethanol solution with the concentration of 50% is added into dendrobium residues extracted by a sodium bicarbonate aqueous solution with the concentration of 1% for re-extraction, gradient separation of components can be realized by using solvent systems with different polarities, and the ethanol solution with the concentration of 50% can dissolve acidic polysaccharide which is not dissolved by alkaline water in dendrobium and can effectively extract medium polar substances such as flavones and glycosides at the same time. In addition, a water-saturated n-butyl alcohol solution is continuously added into the extracted dendrobium residues for extraction, and residual polysaccharide and saponin components in the dendrobium are efficiently extracted. Through the steps, the raw stock components in the dendrobium nobile can be effectively reserved, and the dendrobium nobile has an excellent anti-inflammatory effect.

The invention relates to the technical field of food processing, and discloses a preparation method of sweet and refreshing alfalfa tea and alfalfa tea.The preparation method comprises the steps that high-phenotype catechin alfalfa materials are shaded, and amino acid enrichment is promoted; picking fresh stems and leaves of two-three-leaf tender alfalfa in a squaring stage as raw materials; the raw materials are subjected to temperature-controlled medium-time tedding operation, so that hydrolysis of polysaccharide and protein is promoted, and enrichment of amino acid and soluble sugar is facilitated; the tedded leaves are subjected to rolling stir-frying variable-temperature fixation operation, and degradation of astringent substances such as anthocyanin and flavonoid glycoside is promoted through first high fixation and second low fixation; performing short-time rolling and shaping on the enzyme-deactivated leaves to promote the formation of a curled shape and the overflow of alfalfa juice; the rolled leaves are subjected to hot air-microwave combined drying and heat convection and heat radiation combined operation, so that the taste is sweet and refreshing, and the green color of the alfalfa substitutional tea is kept; and then preparing the sweet and refreshing alfalfa tea. The sweet and refreshing alfalfa substitutional tea is prepared through systematic innovation of alfalfa germplasm resource management, raw material harvesting, a processing technology and the like, and the sweet and refreshing alfalfa substitutional tea can be stably obtained.

This invention discloses a method for determining the flavonoid aglycone content in acid-hydrolyzed sea buckthorn leaf extract, belonging to the field of detection technology. The invention involves acid-hydrolyzing sea buckthorn leaf extract to obtain a filtrate containing flavonoid aglycones, which is used as the test solution. High-performance liquid chromatography (HPLC) is used to detect the flavonoid aglycone content in both the test solution and the reference solution. The preparation method of the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin includes: adding sea buckthorn leaf extract to methanol and hydrochloric acid solutions, mixing thoroughly, heating under reflux, cooling, shaking, and filtering to obtain the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin. This invention simplifies the diverse chemical structures of flavonoid glycosides to a few flavonoid aglycones, effectively reducing dependence on expensive and scarce glycoside reference standards, simplifying chromatographic separation, and improving the stability and reproducibility of the method.

The application belongs to the field of Chinese herbal medicines, and relates to a method for preparing acylated flavonoid glycoside A (quercetin-3-O-[6'''-O-E-p-coumaroyl-beta-D-glucosyl-(1->2)-alpha-L-rhamnose glycoside]) from ginkgo leaf extract. The method first uses macroporous adsorption resin to preliminarily purify the ginkgo leaf extract, carries out decolorization through activated white clay, and then uses flash medium-pressure preparative chromatography and recrystallization means to separate acylated flavonoid glycoside A, so that the purity of the separated acylated flavonoid glycoside A reaches UV >= 98%, ELSD >= 98%, and the recovery rate is high, wherein the acylated flavonoid glycoside A is 70%. The method has the advantages of large preparation amount, high stability, strong repeatability, simple process steps, high purity of separated monomeric compounds, etc., and is an ideal separation process for preparing acylated flavonoid glycoside A from ginkgo leaf extract, and lays a foundation for subsequent industrial scale production.