248 results about "Flavone glycosides" patented technology

It is intended to easily separate saponins from isoflavones extracted from starting soybeans thereby giving highly pure saponins at a high yield on an industrial scale. Saponins can be highly efficiently separated from isoflavones by performing multistage extraction under mild conditions such that malonyl isoflavone glycoside, from among isoflavones contained in the starting soybeans, is not converted into isoflavone glycoside, acetyl isoflavone glycoside or isoflavone aglycon. Thus, highly pure saponins can be obtained at a high yield on an industrial scale.

This is a kind of baicalin total flavones glucoside distillation, baicalin monomer flavones glucoside and its preparation method and application, belonging to the Chinese native medicine drugs manufacturing technology field. The invention obtains the baical in total flavones glucoside distillation mainly containing biacalein, or baical in flavones glucoside monomer compound mainly containing biacalein or wogonoside or oroxylin; comminute baixcal, add water, have alcoholysis reaction at 41 -46 Deg. C, the baialinase and baicalin flavones glucoside naturally existing in baixcal directly have alcoholysis reaction without distillation and separation. The pharmacy preparation includes various pharmacy preparations comprised of officinal auxiliary materials with baicalin total flavones glucoside distillation or monomer flavones glucoside as pharmacy active components, and are used to prepare drugs for curing liver diseases and AIDS. The reaction condition of the invention is mild, and it has high distilling rate, good product quality, low energy waste simple craft, no pollution and low production cost.

The invention relates to total glycosides of Rhodiola rosea, medical application and a preparation method thereof. In the invention, the total glycoside extract prepared from the Rhodiola rosea contains flavone glycosides, phenethyl alcohol glycosides, cinnamyl alcohol glycosides and total terpenoid glycosides, and is mainly characterized by containing Rhodiola rosea flavone (1).

The invention relates to an effective method of extracting puerarin and green food medicines made by it, and the steps include: using alcohol to soak, extracting several times, evaporating the alcohol, converting the extract into water solution, filtering out suspended substances; adopting polymeric absorbent to absorb, concentrating and drying and obtaining Gegen extract, by absorbing and eluting, further making flavone glycoside and terpene inner ester reach the specified indexes. Putting the extracted puerarin in drinks, beers, distilled spirits, teas, milk powders, etc; or adding the puerarin in the tablets, capsules, and electuaries or injectas. The pure plant natural food has proper taste. The Gegen is moderate, peppery and sweet, disperses the internal coldness and relieves the heat of muscle, promotes blood circulation, quenching the thirst and detoxicates alcohol toxicity, etc. and it has good curing effect on high blood pressure, coronary heart disease, heartstroke, dispels the wind, etc.

The invention discloses two types of flavonoid glycoside compounds which are kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(2'' ', 4'' '-O-diacetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-glucopyrose galactopyranoside and kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(4'' '-O-acetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-D-glucopyrose galactopyranoside respectively. The invention also discloses a method for preparing the two types of flavonoid glycoside compounds. Moreover, pharmacological tests prove that the two types of flavonoid glycoside compounds have quite good effects of reducing blood sugar and blood fat and resisting oxidation and cerebral ischemia, and thus, the flavonoid glycoside compounds of the invention can be used for preparing medicaments, food and cosmetics for preventing and treating diabetes, reducing blood fat and resisting oxidation and cerebral ischemia.

The present invention discloses clerodendranthus spiatus extract for treating urinary calculus and its preparation process, and belongs to the field of Chinese herbal medicine extract and application technology. The clerodendranthus spiatus extract preparing process includes the following steps: soaking clerodendranthus spiatus in 4-10 times methanol to extract and concentrating to obtain extractum; dissolving the extractum in solvent in the same volume; extracting the solution first with petroleum ether and then with ethyl acetate and concentrating to obtain coarse extract; and chromatographic separation, eluting, collecting and concentrating to operate clerodendranthus spiatus extract A. The clerodendranthus spiatus extract A is liposoluble flavone glycoside compound and has obvious urinary calculus treating effects.

The invention discloses a jasmine flower extract, a preparation method and an application thereof. The jasmine flower extract contains the following components: 70 percent to 75 percent of jasmine total flavonoids, 15 percent to 20 percent of jasmine non-flavonoid glycosides and 10 percent to 15 percent of jasmine volatile components. A main extraction solvents adopted by the invention when extracting jasmine flower is a mixed solution of ethanol and water in different proportions, and glycoside and aglucon in the flower can also be extracted; polyamide resin column is used for enriching total flavonoids so that impurities are removed and purity is increased; additionally, the method removes a plurality of jasmine fragrant ingredients, and the main effective components of the extract obtained is total flavonoids, the purity is high and the content of jasmine fragrant ingredients is greatly decreased. Pharmacology examination proves that, the extract has the functions of relaxing isolated vascular tissue, removing isolated oxygen free radical, reducing animal blood pressure, relieving myocardial ischemia, improving arrhythmia symptoms and anoxia test and can be applied to treating cardiovascular diseases.

Disclosed is a pharmaceutical preparation for tranquilizing, liver-preserving, allaying fever and relaxing bowels, which comprises 5-95% of iridoid glycosides, 1-90% of saponins, 1-90% of flavones, 1-90% volatile oils, and 1-90% of alkaloids.

The invention relates to a method for extracting plant flavone compounds by an enzymatic process, which comprises the following steps: (1) lixiviating, wherein enzymolysis water extraction is carried out once, and alcohol extraction is carried out twice; (2) purifying on the columns; (3) and processing the analytic solution to obtain the plant flavone compound product. Under the guidance of biotechnology theory, natural product chemical theory and technology and international drug test standard, the invention formulates an experimental scheme for production, and determines proper enzymatic process extraction technique and flavone glycoside biotransformation technique, thereby obtaining the product with high biological value. The invention determines to compound multiple enzyme preparations, optimizes the optimal transformation conditions and enhances the flavone biotransformation efficiency. The invention formulates a technical scheme of adsorption with macroporous absorbent resin to be used in industrial production, thereby increasing the content of aglycon flavones in the product.

Substituted and unsubstituted flavone or isoflavone glycoside derivatives of the formula [A¹-C(═O)O]_m—[X—O-Z]—[O—C(═O)-A²]_n, wherein [X—O-Z] represents a flavone or isoflavone glycoside structure, particularly a naringin residue, X is a flavone or isoflavone corresponding to formula (IIa) or formula (IIb):

wherein the flavone or isoflavone residue is substituted one or more times and/or reduced one or more times; Z represents a mono-, di- or polysaccharide, which is acetally-bound at the benzopyran group to X and ester-substituted by —O—C(═O)-A²; [A¹-C(═O)] is an acyl group on the flavone or isoflavone; A¹ and A² independently, represent a polyunsaturated C_15-26 alkenyl group containing at least four isolated and/or at least two conjugated double bonds, or an arylaliphatic radical with 1-to-4 methylene groups between the ester group and the aromatic ring; [C(═O)A²] is an acyl group; n is an integer other than 0; m is an integer, including 0; and R1, R2 and R are hydroxyl groups or hydrogen atoms.

The present invention relates to a process for isolating plant Argyrobium roseum extract that contains flavonoid glycoside, wherein the extract possesses hypoglycaemic activity. The present invention also contemplates a composition containing the extract and a method of treating various hyperglycaemic conditions including non-insulin dependent diabetes mellitus disease condition by administering the extract.

Total flavone aglycone in baikal skullcap root, which is used in Chinese medicine industry, is produced by: crushing baikal skullcap root, adding water 1-3 times, enzyme hydrolyzing at 37deg.C for 1-4 hours, drying, reflux extracting 1 to 3 times with organic solvent, recovering solvent to obtain total flavone aglycone extracts of baikal skullcap root. Compared with prior arts, its advantages includes: high extractive rate of effective ingredients, high purity and stability, good reproduction quality, simple process, no use for special equipment and suitable for production.

The invention relates to an extract method of Chinese traditional medicinal compound rhizoma coptidis alexipharmic soup active part and an application of the active part in preparing medicament resisting vascular dementia. The extract method disclosed by the invention adopts industrialized separating and refining technologies, such as microporous membrane and macroporous resin absorption, greatly decreases cream yield of the preparation, maximally retains effective components, improves transforming rate of effective components in raw materials and purity of effective components in the preparation. The extracted active part comprises three kinds of known chemical components: alkaloids, flavonoid glycosides and iridoid glycosides respectively, and has a vascular dementia resisting function.

The invention relates to a method for separating and purifying three flavonoid glycosides from trichosanthes bark. According to the method, three high-purity flavonoid glycosides, namely quercetin-3-O-beta-rutinoside, apigenin-7-O-beta-glucoside and diosmetin-7-O-beta-glucoside, are obtained from the trichosanthes bark through the following steps of: (1) preparing a crude trichosanthes bark extract; (2) extracting; (3) carrying out crude separation by using a polyamide column; and (4) carrying out separation and purification by using semi-preparative high-performance liquid chromatography: carrying out separation and purification on total flavonoid glycosides of the trichosanthes bark by using the semi-preparative high-performance liquid chromatography, wherein the mobile phase is methanol-water. The method disclosed by the invention has the advantages that the process flow is environment-friendly, the damage to the environment is not serious, and the comprehensive cost is low.

The present invention discloses a method for separating and preparing high purity flavonoid glycoside compounds from actinidia valvata dunn leaves. The method comprises processes of drug material extraction, crude extract preparation, and monomer compound separation and purification. The drug material extraction process, actinidia valvata dunn leaves are subjected to reflux extraction 2-4 times with ethanol with a volume concentration of 50-80%, and then the ethanol is subjected to vacuum recovering to obtain the extracting solution. In the crude extract preparation process, the extracting solution is loaded on a macroporous adsorption resin, water, ethanol with a volume concentration of 20-30%, and ethanol with a volume concentration of 50-60% are respectively adopted to carry out elution treatments, the eluent after elution with the ethanol with the volume concentration of 50-60% is collected, and is concentrated and dried to obtain the crude extract. In the monomer compound separation and purification process, a high-speed counter-current chromatography method is adopted to separate and purify the resulting crude extract, wherein the concrete steps comprise: preparing a solvent system for forming a stationary phase and a mobile phase, filling the stationary phase in a counter-current chromatography column, rotating the column, pumping the mobile phase into the column, adding the prepared crude extract to an injection valve, and receiving the target flow part according to a detection map.

The method of extracting natural dye from plane tree is to extract natural active dye from bark of plane tree containing resorin A type proanthocyanidin in 22-27 % possessing dyeing activity in the molecular weight range of 350-2000. By means of extracting, adsorbing, refining and other technological steps, coffee dye and pale yellow dye of purity over 90 % are prepared. The obtained dyes have high dyeing performance and high fastness on hair, wool and fabric, and will have raised use effect when being compounded with hydrolyzed tannin or flavone glycoside dye. The obtained dyes are used in dyeing hair, wool, fabric, etc.

The invention discloses a method for preparing total ginkgo flavone glycosides slow-release capsules by applying attapulgite, which comprises the following steps of: washing, drying and pulverizing gingko leaves into coarse powder; adding the gingko leaf coarse powder and petroleum ether with the weight ten times of the gingko leaf coarse powder into a container, refluxing and extracting for 1 hour, degreasing, filtering and drying; adding water with the weight ten times of the degreased gingko leaf coarse powder into the degreased gingko leaf coarse powder, boiling, decocting for 3 times, combining decocting liquids, filtering and concentrating to the relative density of 1.10; adding ethanol with the mass concentration of 95 percent into a concentrated liquid until the ethanol concentration is 70 percent, depositing, settling and filtering; adsorbing a filtrate through modified attapulgite to obtain the attapulgite loading total ginkgo flavone glycosides; and adding a pharmaceutical adjuvant material into the attapulgite loading total ginkgo flavone glycosides, and encapsulating into empty capsules to obtain the total ginkgo flavone glycosides slow-release capsules. The invention adopts the principle of adsorption separation and selects the modified attapulgite with strong adsorbability for the total ginkgo flavone glycosides to directly prepare a preparation without elution, the total ginkgo flavone glycosides slow-release capsules are slowly desorbed under the desorption action of a body fluid, steps are simplified, the production period is shortened, and the yield of products is improved.

A deacidified ginkgo leaf and its extract are disclosed. The deacidified ginkgo leaf contains flavone glycoside and terpene lactone, but almost not contains alkylphenate of original ginkgo leaf. The flavone glycoside and terpene lactone can be extracted by organic solvent or supercritical CO2 method. The deacidified ginkgo leaf can be used to prepare medicine for preventing and curing coronary heart disease, angine pectoris, cerebral thrombus and cerebral disfunction, health-care food and health-care tea.

The invention discloses an oral preparation of ginkgo leaf total flavonoid aglycone, a preparation method and an application thereof, pertaining to the field of traditional Chinese medicine. Total flavonoid glycosides extracted from ginkgo leaves are hydrolyzed to obtain ginkgo leaf total flavonoid aglycone which is then added with special auxiliary materials for preparing a highly efficient preparation, thereby greatly increasing the bioavailability of the ginkgo leaf total flavonoid aglycone. The preparation has significant curative effects in the treatment of coronary disease, hyperpiesis, cerebral thrombosis, cerebral infarction, apoplexy, dizziness, psychosis, nephritis, bronchitis, Parkinson's disease, diabetes, tumor, leukemia and other diseases.

The invention discloses a method for extraction of total flavonoid aglycones from hickory leaves. The method comprises: subjecting hickory leaf dry powder to heating reflux extraction by an ethanol aqueous solution with a volume concentration of 50-95%, conducting filtering to obtain a total flavonoid aglycone extract solution; conducting a pretreatment on polyamide; adding the pretreated polyamide into the total flavonoid aglycone extract solution, adding water and performing vacuum distillation to obtain a concentrated mixed solution, directly applying the mixed solution on a column, and letting the solid polyamide settle naturally to a smooth and bubble-free state; releasing the liquid from the column, then first employing an ethanol aqueous solution with a volume concentration of 20-30% to wash away impurities, then using an ethanol aqueous solution with a volume concentration of 40-50% as an eluent to perform elution, collecting the eluent, and evaporating the eluent, thus obtaining the total flavone glycosides. The total flavone glycosides obtained by the method provided in the invention has the advantages of: high purity and high recovery rate. In addition to simple process and convenient operation, the method also has a low cost, and is suitable for industrialized production.

The invention discloses an extraction technology of plant essential oil, flavone and polysaccharide. The extraction technology comprises the steps of extracting the plant essential oil, the flavone and the polysaccharide for three times under ultrasonic assistance by taking an alcohol aqueous solution containing cellulase as an extraction solvent, merging extracts, carrying out centrifugal separation to form an essential oil crude product, a water phase and a flavone aglycone crude product after reduced pressure distillation to recover alcohol, Extracting flavone glycoside from the water phase by using n-butyl alcohol, dissolving flavone aglycone in an extraction phase containing flavone glycoside, carrying out the reduced pressure distillation to recover n-butyl alcohol, drying to form a flavone crude product, and carrying out alcohol precipitation, filtering and dryness on raffinate containing polysaccharide to form a polysaccharide crude product. The extraction technology makes full use of a plant raw material and has the advantages of simplicity, safety, high product yield, low damage probability of activity and the like.

The present invention provides camellia flavonoid glycoside A with estrogenic activity, a preparation method and applications thereof. The compound is a novel compound prepared by camellia semiserrata Chi seed oil meal, the system of which is named as kaempferol-3-O-Beta-D-glucosyl-(6.1)-2'', 4''-O-diacetyl-Alpha-L-rhamanopyranosyl-(3.1)-2'', 3'', 4''-O-triacetyl-Alpha-L-rhamnoside. The compound has obvious estrogenic activity.

The invention relates to a flavonoid glycoside compound in flos elaeagni angustifoliae, and a preparation method thereof. The method comprises the following steps: taking flos elaeagni angustifoliae medicines as raw materials, extracting by solvent, extracting by solvent, carrying out separation through two or three ways including positive phase and reverse phase silica gel column chromatography,or glucan gel LH-20 column chromatography and a semi-preparation high-performance liquid chromatography, adopting thin-layer chromatography and the high-performance liquid chromatography to detect andanalyze to obtain two new flavonoid glycoside compounds, wherein the first compound is flos elaeagni angustifoliae glycoside A, and the second compound is flos elaeagni angustifoliae glycoside B. Through the measurement of in vitro inhibition of COX (cyclo-oxygense) enzyme activity, an experiment result indicates that the flavonoid glycoside compound separated from the flos elaeagni angustifoliaecan inhibits the COX enzyme activity in different degrees, and the flavonoid glycoside compound in flos elaeagni angustifoliae can be used for preparing anti-inflammatory medicines.

Belonging to the technical field of extraction and separation, the invention relates a preparation method and application of two active flavonoid glycosides in okra fruits. With okra fruits as the raw materials, the method comprises the steps of: transferring out polysaccharide and pectin through percolation extraction, flash evaporation and condensation, extraction and decolouration, alcohol precipitation, carrying on vacuum condensation, separating enrichment by a macroporous adsorption resin column, then conducting separation by combing the gel resin column chromatography technology, thus obtaining two monomer compounds of 5, 7, 3', 4'-tetrahydroxy-4''-O-methyl-3-O-beta-D-glucose flavonoid glycoside and 5, 7, 3', 4'- tetrahydroxy-3-O-[beta-D-glucosyl-(1-2)]-beta-D-glucose flavonoid glycoside, with extraction rates of 3.62%-8.66% and 8.01%-12.98% respectively. The purity of the two compounds is both above 95%. The method of the invention has simple process and low production cost, and the two flavonoid glycosides are characterized by good antioxidation and bacteriostatic activity.

The invention relates to a method for quickly separating a high-purity cowherb seed flavonoid glycoside monomer from a Chinese medicinal material cowherb seed and belongs to the technical field of Chinese medicines. The method comprises the following process steps of: A, extracting raw materials, namely poaching; B, enriching by using macroporous resin; C, performing high-efficiency preparation and liquid phase separation, wherein C18 fillers are adopted, the detection wavelength is 280 nm and the mobile phase is a 30 volume percent methanol solution; and D, recovering products, namely enriching a preparation solution by using the macroporous resin, desorbing by using ethanol, concentrating and drying to obtain the cowherb seed flavonoid glycoside monomer with the purity of over 99 percent. The method is simple and quick, low in solvent consumption, high in yield, and economic and environment-friendly. A large number of products can be prepared by the method at one time. The method has high economic value and academic value.