12 results about "Flavone glycosides" patented technology

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

This invention relates to the field of plant tissue culture and the breeding of superior varieties of traditional Chinese medicinal materials. This invention discloses a method for rapid propagation of Epimedium inflorescences via tissue culture, comprising the following steps: S1, collecting newly sprouted flower inflorescences of Epimedium in early spring, disinfecting them, and setting them aside; S2, cutting the disinfected inflorescences into 1.1-1.2 cm segments, inoculating them into callus induction medium, and culturing them under conditions of 1200-1300 lux light intensity, 12 h / day photoperiod, and 24℃. This invention ensures uniform seedling quality and stable traits through targeted screening of effective components and monitoring of genetic stability. The optimized culture medium ratios at each stage result in a high proliferation coefficient, robust rooting, and a short propagation cycle, enabling efficient year-round propagation. Standardized seedling hardening and transplanting lead to a high survival rate. This method is technically stable and highly applicable, enabling the mass production of high-flavonoid glycoside, genetically stable, high-quality seedlings, fundamentally solving problems such as germplasm degradation, low propagation coefficient, and uneven quality, providing strong support for the breeding and industrialization of superior varieties of Epimedium.

The present application relates to the technical field of traditional Chinese medicine detection, and provides a genuine three-leafed knot thin-layer identification kit and method, comprising: a chrysophanol control solution, a neochlorogenic acid control solution and a rapid extraction solvent, the rapid extraction solvent contains a low-carbon alcohol, which is used for extracting flavonoid glycoside components in three-leafed knot stem and leaf samples, and the kit is used for identifying the origin of three-leafed knot by detecting the content of chrysophanol and the presence of neochlorogenic acid. The present application uses a chrysophanol and neochlorogenic acid double-index mutual exclusion identification logic, combined with a rapid extraction solvent system without drying, to realize on-site, rapid and accurate identification of the genuineness of three-leafed knot, and effectively solves the problem of origin confusion.

A method for decomposing flavonoid glycosides, comprising heating a raw material containing flavonoid glycosides in a sealed container in the presence of alcohol at a temperature exceeding the boiling point of the alcohol at normal pressure, thereby decomposing the flavonoid glycosides into flavonoids.

This invention discloses a method for determining the flavonoid aglycone content in acid-hydrolyzed sea buckthorn leaf extract, belonging to the field of detection technology. The invention involves acid-hydrolyzing sea buckthorn leaf extract to obtain a filtrate containing flavonoid aglycones, which is used as the test solution. High-performance liquid chromatography (HPLC) is used to detect the flavonoid aglycone content in both the test solution and the reference solution. The preparation method of the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin includes: adding sea buckthorn leaf extract to methanol and hydrochloric acid solutions, mixing thoroughly, heating under reflux, cooling, shaking, and filtering to obtain the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin. This invention simplifies the diverse chemical structures of flavonoid glycosides to a few flavonoid aglycones, effectively reducing dependence on expensive and scarce glycoside reference standards, simplifying chromatographic separation, and improving the stability and reproducibility of the method.

A method for producing a flavone inclusion compound, comprising a detachment step of treating a poorly soluble flavone having a rhamnose glycoside structure with an enzyme having rhamnodiastase activity in the presence of a cyclodextrin to detach rhamnose. According to the production method of the present invention, a flavone inclusion compound, a flavone glycoside composition, which is excellent in solubility in water, can be efficiently produced, and can be suitably utilized in the fields of pharmaceuticals, food and drink, health foods, foods for specified health uses, and cosmetics, etc.

The application discloses a flavone glycoside compound and application thereof in preparation of anti-inflammatory drugs, and the application comprises the following steps: step one, alcohol extraction of raw materials; step two, resin column chromatography; step three, silica gel column chromatography; step four, polyamide column chromatography; step five, ODS column chromatography; step six, obtaining of the target compound; and step seven, application of the target compound; in the step one, the alcohol extraction process specifically comprises: 2-4 times of heating reflux extraction, each time for 1-3 hours; in the step six, the separation method is HPLC high performance liquid chromatography; the flavone carbon glycoside compound with anti-inflammatory activity is obtained through the following steps of solvent extraction, macroporous adsorption resin column chromatography, silica gel column chromatography, polyamide column chromatography, ODS column chromatography and preparation liquid phase separation by using dried flowers of the Trollius chinensis G.Lodd, and the application has the advantages of simple separation method and easy-to-obtain raw materials, and the obtained compound is expected to be developed into an anti-inflammatory lead compound, and has important significance for development of anti-inflammatory drugs.

This invention provides a glycosyltransferase ThUGT1, the nucleotide sequence of which is shown in SEQ ID NO.1. This invention also provides a method for synthesizing flavonoid compounds. This invention provides the flavonoid glycosyltransferase ThUGT1 gene from *Trifolium repens*, which can serve as a regulatory gene for the biosynthesis of components such as luteolin, kaempferol, and genistein, and can be applied to the preparation of luteolinoside, kaempferol-7-O-glucoside, and genistein. This invention utilizes a heterologous expression method to biosynthesize glycosides through microbial biosynthesis, and also verifies its function and enzymatic properties. The method exhibits strong site specificity, reacting only at position 7, good solubility, and a broad substrate scope, binding to most flavonoid compounds, especially the relatively scarce 7-O-glucoside transferase. The resulting mutant can improve the yield, enabling industrial production, promoting the development of *Trifolium repens* glycosides, and providing a foundation for the widespread application of *Trifolium repens*.

The application discloses a jellyfish saussurea extract with acetylcholinesterase inhibitory activity and a preparation method thereof, and relates to the technical fields of traditional Chinese medicine extraction and nerve drugs. The jellyfish saussurea extract is prepared by the following steps: drying jellyfish saussurea whole grass, crushing, sieving, obtaining jellyfish saussurea coarse powder, adding an acetic acid-sodium acetate buffer solution with a pH value of 4.5-5.0 to the coarse powder, and the adding amount of the buffer solution is 1:10-1:20 (w / v). A composite enzyme is added to the above-mentioned mixed system, and the composite enzyme is composed of cellulase and pectinase, and the mass ratio of the two is 1.5-2.5:1. The application adopts the integration of "composite enzymatic wall breaking, double-frequency ultrasonic assisted extraction, and macroporous resin directional elution", and the AChE inhibitory active ingredients (small molecule phenolic acid and flavonoid glycoside) in the jellyfish saussurea are directionally enriched according to the physical and chemical properties. The composite enzymatic wall breaking (cellulase+pectinase) can efficiently degrade plant cell wall cellulose and intercellular layer pectin, and significantly improve the wall breaking rate.

This invention discloses a method for detecting and separating eight structurally similar flavonoid glycosides using capillary electrophoresis, belonging to the field of biochemical analysis technology. The invention includes the following steps: First, prepare borate stock solution, cyclodextrin phosphate buffer, DES background buffer solution, and mixed standards for later use; employ a capillary electrophoresis system equipped with a diode array detector, using uncoated fused silica capillary tubes with an inner diameter of 75 μm and an effective length of 60 cm; pre-treat the capillary tubes, perform separation and detection, obtain the electrophoretic peak diagram of the sample, extract the peak area values ​​of each peak, calculate the migration time and concentration of each component, and achieve the separation and quantification of eight flavonoid glycosides. The buffer solution used in this invention can perfectly separate flavonoid glycosides that cannot be separated by HPLC due to structural similarity; all eight flavonoid glycosides elute within approximately 12 minutes with symmetrical and well-separated peaks.

The application discloses a flavonoid aglycone extraction and separation method based on metal ion doped eutectic solvent, relates to the technical field of natural product extraction and separation, and comprises the following steps: mixing a hydrogen bond acceptor, a hydrogen bond donor and a metal ion according to a molar ratio to synthesize a metal ion doped eutectic solvent, and adding water to obtain an MTDES aqueous solution; adding plant powder containing flavonoid aglycone into the MTDES aqueous solution, performing ultrasonic extraction, centrifugal separation, collecting supernatant, and obtaining an extract; mixing the extract with a water-soluble small-molecule organic solvent, adjusting the pH and / or the concentration of salt, centrifugally separating phases, collecting an organic solvent phase, and concentrating to obtain a flavonoid aglycone crude extract. 3+ The application utilizes the catalysis of Al 3+ and the synergistic effect of the DES hydrogen bond network to realize the integration of in-situ hydrolysis of flavonoid glycosides and extraction of aglycone. Compared with the traditional acid hydrolysis-organic solvent extraction process, the method has the advantages of simplified steps, shortened time and reduced solvent consumption.