42 results about "Gibberella acuminata" patented technology

The invention relates to the field of biology, in particular to a primer probe combination for identifying wheat blast, a product and application of the primer probe combination. The invention provides a primer and probe combination for identifying wheat blast. The primer and probe combination comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO.4, a downstream primer with a nucleotide sequence as shown in SEQ ID NO.5 and a probe with a nucleotide sequence as shown in SEQ ID NO.6. The primer probe combination of the specific marker is designed based on the gene of the pyricularia grisea, and the primer probe combination can accurately distinguish the pyricularia grisea, the gibberella and the pyricularia oryzae. Meanwhile, based on the designed primer probe combination, the pestilence pyricularia, gibberella and magnaporthe oryzae are respectively detected by utilizing an RPA amplification technology and a nucleic acid colloidal gold detection test strip, and the detection method can be used for specifically detecting the pestilence pyricularia.

The present invention relates to the biological field, and in particular to a primer-probe combination, product and application thereof for identifying wheat blast. The present invention provides a primer-probe combination for identifying wheat blast, the primer-probe combination comprising an upstream primer having a nucleotide sequence as shown in SEQ ID NO.4, a downstream primer having a nucleotide sequence as shown in SEQ ID NO.5 and a probe having a nucleotide sequence as shown in SEQ ID NO.6. The present invention designs a specifically labeled primer-probe combination based on the wheat blast fungus gene, and the primer-probe combination can accurately distinguish wheat blast fungus, gibberellosis and rice blast fungus. At the same time, based on the designed primer-probe combination, the present invention uses RPA amplification technology and nucleic acid colloidal gold detection test strips to detect wheat blast fungus, gibberellosis and rice blast fungus respectively, and the detection method can specifically detect wheat blast fungus.

The invention discloses an application of transmembrane transporters NPF and SWEET in increasing the yield of gibberellin of Fusarium fujikuroi, and the application comprises the following steps: performing heterologous expression on an exogenous transmembrane transporter NPF or SWEET in wild type Fusarium fujikuroi in a manner of constructing an integrated expression plasmid, so as to increase the yield of gibberellin GA4 + 7. A new thought is provided for increasing the yield of gibberellin, the yield of GA4 + 7 is effectively increased through heterologous overexpression of the transmembrane transporter NPF or SWEET, gibberellin which is more efficient, more stable, safer and higher in economic value is obtained, and the gibberellin has a positive promotion effect on industrial production of gibberellin.

The present invention relates to methods for controlling or preventing infestation of a rice plant by the phytopathogenic microorganism Gibberella fujikuroi, comprising applying to a crop of rice plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The invention provides a genetically engineered bacterium for producing gibberellin GA4 + 7 as well as a construction method and application of the genetically engineered bacterium. According to the genetically engineered bacterium, a CRISPR-Cas9 gene editing technology is adopted, a truncated mevalonyl coenzyme A reductase encoding gene tHMGR is inserted into a P450-3 gene locus in a Fusariumfujikuroi genome of a chassis bacterium, and POT1, FOX1 and FAA1 genes are further inserted into a fsr1 locus, a fsr3 locus and a fsr4 locus respectively, so that the reinforcement of a beta oxidation pathway is realized. The genetically engineered bacterium improves the supply capacity of a precursor acetyl CoA in the process of producing gibberellin GA4 + 7, and the yield of GA4 + 7 after fermentation of a fermentation medium for 168 hours reaches 2.66 g / L and is improved by 443.33% compared with the yield of an original strain GA4 + 7.

The invention discloses bacillus subtilis and application thereof, and belongs to the field of microorganisms. The technical problem to be solved by the invention is how to inhibit pathogenic bacteria by a biological control method. Therefore, the invention provides the bacillus subtilis, the bacillus subtilis is bacillus subtilis, the strain number of the bacillus subtilis is WR4, and the preservation number of the bacillus subtilis in the China General Microbiological Culture Collection Center is CGMCC No.29148. The invention further provides a preparation method of the bacillus subtilis. The bacillus subtilis CGMCC29148 provided by the invention is separated from rice rhizosphere soil, and the bacillus subtilis CGMCC29148 and the rice rhizosphere soil coexist in a micro-ecological harmonious manner; the strain has certain salt tolerance, can be applied to a wider range of soil, and can be used for widely preventing and treating pathogenic fungi such as fusarium, verticillium dahliae, magnaporthe oryzae and gibberella zeylanica and pathogenic bacteria such as potato scab.

The present invention discloses a seed endophyte for preventing and treating wheat fungal diseases and its application, belonging to the technical field of crop planting and crop disease biological control. The wheat endophyte (Pseudescherichia vulneris) GSE25 in the present invention was deposited in the China General Microbiological Culture Collection Center (CGMCC) on November 21, 2022, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.26158. The GSE25 strain has the ability to significantly reduce the pathogenicity of gibberellins and stripe rust on wheat plants, and can significantly reduce the mycotoxins produced by pathogens on wheat grains, has a promoting effect on crop growth and development, and has a broad-spectrum effect. The GSE25 strain of the present invention is of great value in the healthy growth, yield increase, control of pathogenic fungi and mycotoxin pollution of crops.

The present invention discloses a 1-((piperidin-4-yl)amino)propan-2-ol compound, its preparation and application. The structural formula of the 1-((piperidin-4-yl)amino)propan-2-ol compound is shown in Formula I. The compound of the present invention has a low cost and is easy to synthesize, and can be used as an inhibitor of trehalose-6-phosphate synthase in the prevention and control of crop diseases, especially the prevention and control of crop pathogenic bacteria represented by Magnaporthe oryzae, Fusarium graminearum and Botrytis cinerea.

The utility model relates to the technical field of gibberella fermentation, in particular to a waste residue treatment device for gibberella fermentation, which comprises a support table, an outer cylinder is mounted at the top of the support table in a penetrating manner, a cover plate is placed at the top of the outer cylinder, a support frame is connected to the center of the top of the cover plate, and a variable frequency motor is mounted on the support frame in a penetrating manner. The output end of the variable frequency motor and the interior of the outer cylinder are jointly connected with a processing part. According to the utility model, the variable frequency motor runs slowly, and meanwhile, the telescopic end of the electric telescopic rod II extends out to further open the electromagnetic valve on the discharge pipe; when the telescopic end of the second electric telescopic rod corresponds to the arc-shaped notch, the follow-up variable frequency motor runs at a high speed, so that liquid in the gibberella fermentation waste residues is thrown out and discharged from the discharging pipe. The gibberellic acid fermentation waste residues are crushed by the main heating crushing rod and the auxiliary heating crushing rod, and the gibberellic acid fermentation waste residues are dried by the heating column, the main heating crushing rod and the auxiliary heating crushing rod.

The invention provides a genetically engineered bacterium for producing gibberellin GA3 at high yield, a construction method and application. According to the invention, a key phospholipid synthesis regulation factor ino2 in gibberella zeylanica is enhanced and expressed, and a transcription inhibition factor opi1 and a pah1 gene for coding phosphatidic acid phosphatase are knocked out by adopting a CRISPR-Cas9 gene editing technology, so that the area amplification of the endoplasmic reticulum is stimulated, the synthesis and folding capabilities of endoplasmic reticulum protein are enhanced, and the metabolic limitation of limited enzyme abundance is relieved; and the yield of GA3 is further improved. Compared with a wild type, the reformed recombinant gibberella fujikuensis has the advantages that the endoplasmic reticulum area is enlarged, so that enrichment of recombinant terpenoid biosynthase in the endoplasmic reticulum is stimulated, accumulation of GA3 is finally improved, the gibberellin GA3 production level of a strain with the optimal performance is improved by 31.5% compared with that of an original strain, the gibberellin GA3 production capacity is remarkably improved, and the production cost is reduced. And a feasible route is provided for construction of high-yield GA3 engineering strains.

The present application relates to the field of microorganism, discloses a kind of fusarium fujikuroi, the fungicide containing the bacteria and their application and the method for producing gibberellin.The preservation number of the fusarium fujikuroi is CCTCC NO:M 20221848.The present application further provides the application of the fusarium fujikuroi in producing gibberellin.The method for producing gibberellin includes the following steps: the fusarium fujikuroi and / or the fungicide is inoculated into seed culture medium to carry out seed culture and obtain seed liquid;The seed liquid is inoculated into fermentation culture medium to carry out fermentation culture.The fusarium fujikuroi provided by the present application can resist gamma ray irradiation, has good environmental adaptability and gibberellin production potential, and is suitable for industrial production.

The invention relates to methods for controlling or preventing infestation of a plant by the phytopathogenic microorganism Phyllachora maydis, comprising applying to the phytopathogen, to the locus of the phytopathogen, or to a plant susceptible to attack by the phytopathogen, or to a propagation material thereof, a fungicidally effective amount of a compound as defined in claim 1.

The invention relates to the field of gene engineering, and discloses a CRISPRi (clustered regularly interspaced short palindromic repeats i) system, a recombinant strain containing the CRISPRi system, a construction method and application of the recombinant strain, and a method for producing gibberellin. The CRISPRi system comprises a coding gene of a dCas9 mutant protein without cleavage activity, sgRNA and an induction regulation component, wherein the induction regulation component is used for regulating the expression of the dCas9 mutant protein coding gene; the sgRNA guides the dCas9 mutant protein to be combined to a target gene, so that the expression of the target gene is inhibited; the dCas9 mutant protein is obtained by mutation on the basis of a Cas9 protein, and mutation sites are D10A and H840A of the dCas9 protein; the target genes comprise squalene synthase encoding genes, fatty acid synthase encoding genes and the like, the CRISPRi system can accurately and efficiently regulate and control gibberellin gene expression, the gibberellin yield is remarkably increased, the influence on strain growth is small, regulation and control are reversible, specificity is high, and the CRISPRi system is matched with gibberellin and has good industrial prospects.

The invention provides a gibberellin fermentation process. The gibberellin fermentation process comprises the following steps: step 1, strain activation: taking out a gibberellic fungus strain from a low-temperature refrigerator at-80 DEG C, transferring the strain to a PDA culture medium, and culturing for 7-8 days to obtain an activated seed solution; step 2, liquid seed culture: punching a fungus cake with the diameter of 10mm on the same circumference of the activated PDA culture medium plate by using a sterile puncher, putting the fungus cake into a test tube filled with sterile water and glass beads, putting the test tube into a vortex oscillator, oscillating for 10min to obtain a fungus suspension, adding the fungus suspension and a seed culture medium into a shake flask, putting the shake flask into a constant-temperature oscillator, and shaking for 10min to obtain a seed culture medium; performing constant-temperature shaking culture for 48 hours under the conditions of 33 DEG C and 220rpm to obtain liquid seeds; step 3, GA4 fermentation: inoculating the liquid seeds onto a palm oil fermentation culture medium, putting the inoculated culture medium into a constant-temperature oscillator, performing constant-temperature shaking culture under the conditions of 33 DEG C and 220rpm, and setting two control groups; the method has the advantage of improving the fermentation yield of GA4.

The application discloses a recombinant Gibberella fujikuroi for blocking competitive metabolic branch to improve gibberellin yield and a construction method thereof, and belongs to the technical field of industrial microorganisms.The recombinant Gibberella fujikuroi provided by the application knocks out a p450-3 gene, the GA4 yield of the recombinant strain is increased to 718.6 mg / L from 585.9 mg / L of the original strain, is increased by 22.6%, and the growth characteristics of the strain are not significantly affected.Through blocking the 13-hydroxylation competitive branch, the application realizes the redirection of metabolic flow to non-13-hydroxylation product GA4 / 7, provides an efficient chassis strain and a construction method for directional production of gibberellin components, and has important industrial application value.

The invention provides genetic engineering construction and application of a gibberellin GA4 + 7 efficient production strain. According to the method, the speed limiting step of a metabolic pathway of gibberella zeylanica is explored, key genes fpps, gs2, cps / ks and thmgr of the gibberella zeylanica are subjected to editing expression, the screened key genes ggs2, cps / ks and thmgr are subjected to co-expression, screening markers are recycled by using an inducible recombinase-based gibberella zeylanica screening marker recycling system, and finally, the strain OE: thmgr-gs2-cps / ks with the highest yield is obtained. The yield of GA4 + 7 in an optimized fermentation culture medium reaches 3.6 g / L.

The present invention provides a potential drug target protein PPTase and its encoding gene for use in the preparation of drugs for preventing and treating plant fusarium rust. ppt1 After the gene was removed, the production of GA3, GA4, and GA7 in gibberellins decreased significantly; at the same time, the growth ability of the knockout bacteria on PDA, MM, SM and other media decreased significantly. Therefore, inhibitor drugs designed with proteins as targets at this site can prevent and control diseases caused by gibberellin-producing fungi in plant fungi, providing a new way to screen fungal drugs for the prevention and control of plant fusarium rust.

The application provides application of a ppt1 gene in high-yield gibberellins GA4 and GA7. The method of the application is that an expression frame of the ppt1 is expressed in filamentous fungi by means of constructing an expression plasmid. By overexpressing the important gene ppt1 in the lysine synthesis pathway in filamentous fungi, the optimized strain only produces the intermediate metabolite GA4 and GA7, and does not produce the end metabolite GA3. The method is simple in operation and remarkable in effect, and provides a new way for construction of the high-yield GA4 and GA7 engineering strain. Compared with the wild type Gibberella fujikuroi, the Gibberella fujikuroi engineering strain provided by the application can more efficiently produce the fermentation products of GA4 and GA7, so that the GA 4+7 The mixture yield is increased by 36 times.

The invention relates to the field of gene engineering, and discloses a CRISPR-dCas9 system, a recombinant strain containing the CRISPR-dCas9 system, application of the CRISPR-dCas9 system and a gene editing method, and the CRISPR-dCas9 system comprises 5SrRNA, sgRNA, an encoding gene of adenine deamination mutant enzyme, a Linker sequence I and an encoding gene of dCas9 protein in the direction from upstream to downstream; wherein the 3'end of the adenine deamination mutant enzyme coding gene is connected with the 5 'end of the dCas9 protein coding gene through a Linker sequence I, and the sgRNA and the 5SrRNA are respectively an endogenous guide sequence and an endogenous promoter of gibberella fujiangjensis; the adenine deamination mutant enzyme is obtained by mutation on the basis of adenine deamination enzyme, and mutation sites are T111R and D119N of the adenine deamination enzyme; the nucleotide sequence of the Linker sequence I is as shown in any one of SEQ ID NO. 5, SEQ ID NO. 39 and SEQ ID NO. 40. The CRISPR-dCas9 system provided by the invention is suitable for gibberella zeylanica, provides an efficient tool for gene function research and genetic modification of gibberella zeylanica, and has relatively high industrial potential.

The invention relates to the technical field of microorganisms and application thereof, and discloses a piggyBac transposon system, a recombinant vector, a gene editing method, a recombinant strain and application of the piggyBac transposon system, the piggyBac transposon system contains piggyBac transposase, an inverted terminal repeat sequence, a target load gene sequence and a selective marker gene, the vector gene is a functional marker gene and / or a reporter gene, and the expression of the transposase is controlled through a promoter PtrpC; wherein the amino acid sequence of the piggyBac transposase is as shown in SEQ ID NO. 1; and the piggyBac transposase system also contains a vector plasmid. The gene editing method of the piggyBac transposon system can be suitable for efficiently and accurately inserting exogenous genes into gibberella zeylanica, traceless and residue-free genome modification is realized, the problem of exogenous gene residue is avoided, and the method has relatively good industrial value.

The invention belongs to the field of industrial microorganisms, and particularly relates to gibberella fujikubin and application and a fermentation product thereof. The preservation number of the gibberella zeylanica is CCTCC NO: M 20251933, the gibberella zeylanica is used for fermentation production of terpene derivatives, and the terpene derivatives can be used for preparation of plant growth regulators. The technology provides important lead compounds and technical platforms for development of green plant growth regulators, along with deep popularization of green agriculture and sustainable development concepts, the plant growth regulators with natural sources have more and more important application values, and the technology has wide industrial application prospects.

The invention discloses a dimethoxy xanthone compound and application thereof, and belongs to the technical field of agriculture. The invention finds that the dimethoxy xanthone compound shown in the formula (1) has a strong inhibition effect on phytopathogens, especially on pyricularia oryzae, wheat gibberella and phytophthora; the compound 1 can be used for bactericides, has a very high pesticide research value, and has a wide application prospect in the field of agricultural pharmacology.

The invention discloses a 2 (5H)-furanone derivative, a preparation method thereof and application of the 2 (5H)-furanone derivative as a biocide. The invention provides an effective means for synthesizing the 2 (5H)-furanone derivative, when the 2 (5H)-furanone derivative is prepared, the reaction conditions are green and metal-free, the operation is easy at room temperature, and the large-scale production is facilitated; according to the invention, the target product can be obtained with high yield and high selectivity. The 2 (5H)-furanone derivative disclosed by the invention has good inhibitory activity on the mycelial growth rate of plant-derived germs (such as tomato early blight, strawberry grey mould and wheat gibberellic mildew), has bactericidal activity and can be used as a pesticide.

The invention relates to the technical field of metabolic engineering, and discloses a by-product eliminated gibberellin high-yielding strain as well as a construction method and application thereof. In gibberella zeylanica, a bicarpin gene cluster and a fusarium gene cluster are relatively large, the size of the bicarpin gene cluster is about 17 kb, and the size of the fusarium gene cluster is about 16.8 kb, so that the difficulty of knocking out the bicarpin gene cluster and the fusarium gene cluster at the same time is relatively large. According to the present invention, with the CRISPR-Cas9 gene editing technology, the simultaneous knockout of the bicarpin gene cluster and the fusarium gene cluster is achieved, such that the influence of the fermentation by-product bicarpin and fusarium is eliminated, the gibberellin synthesis is increased, and the high-yield gibberellin gene engineering strain is obtained.

The invention provides a high-yield gibberellin GA4 + 7 genetically engineered bacterium and a construction method and application thereof.The genetically engineered bacterium is constructed with gibberellic acid as a chassis, and the construction method comprises the steps that P450-2 and CPR genes are inserted into a bik2 site through a CRISPR-Cas9 gene editing technology, and carbon flux flows to synthesis of GA4 + 7; the Hmgs, Erg10 and Ggs2 genes are subjected to combined overexpression; and superposing and over-expressing endogenous Fpps and Cps / Ks genes. Compared with an original strain, the modified genetic engineering strain can better utilize starch hydrolysate as a carbon source to synthesize gibberellin GA4 + 7, the GA4 + 7 production titer is improved to 4.49 g / L after shake flask fermentation for 168 hours and is improved by 68.80% compared with the original strain, and the modified genetic engineering strain has important industrial application value.

The invention provides an application of a ppt1 gene in high yield of gibberellin GA4 and GA7. The application method comprises the step of expressing the ppt1 expression cassette in filamentous fungi in a manner of constructing expression plasmids. Through overexpression of an important gene ppt1 in a lysine synthesis route in filamentous fungi, the optimized strain only produces intermediate metabolites GA4 and GA7, and does not produce a metabolic end product GA3. The method is simple to operate and remarkable in effect, and a new way is provided for construction of high-yield GA4 and GA7 engineering strains. Compared with wild gibberellic gibberellic acid, the gibberellic gibberellic acid genetically engineered bacterium provided by the invention can more efficiently perform fermentation production of GA4 and GA7, so that the yield of a GA4 + 7 mixture is increased to 36 times.

The application discloses application of a transmembrane transporter NPF or SWEET in improving gibberellin yield of Fusarium fujikuroi, and the application comprises the following steps: heterologously expressing an exogenous transmembrane transporter NPF or SWEET in wild-type Fusarium fujikuroi by constructing an integrative expression plasmid, and then improving the gibberellin GA 4+7 yield. The application provides a new idea for improving the gibberellin yield, effectively improves the GA 4+7 yield by heterologously overexpressing the transmembrane transporter NPF or SWEET, and obtains more efficient, more stable, safer and more economically valuable gibberellin, and has a positive promoting effect on industrial production.

The application provides a naringin derivative, a preparation method of the naringin derivative, and application of the naringin derivative in preparation of medicines for preventing and treating wheat scab, tobacco black stem disease, phomopsis stem rot and pepper wilt, and has good bacteriostatic activity on wheat scab fungus, tobacco black stem fungus, phomopsis stem rot fungus and pepper wilt fungus, has a good prevention and treatment effect on plant pathogenic fungal diseases such as wheat scab, tobacco black stem disease, phomopsis stem rot and pepper wilt, and has a broad application prospect and development potential.

The present invention discloses a method for converting progesterone into 11α, 17α-diOH-progesterone by utilizing Gibberella intermedia, belongs to the field of biotechnology, and is particularly related to a method for converting progesterone into 11α, 17α-diOH-progesterone by utilizing Gibberella intermedia CA3-1. The strain is deposited in the General Microbiological Center of China Committee for the Collection of Microorganisms, with a deposit number of CGMCC No.4903. Gibberella intermedia CA3-1 can convert progesterone into 11α, 17α-diOH-progesterone. When the substrate feed concentration is 3g / L, the concentration of 11α, 17α-diOH-progesterone is 0.626g / L, and the conversion rate of progesterone into 11α, 17α-diOH-progesterone is 18.9%. At present, the synthesis of 11α, 17α-diOH-progesterone provides a more diversified precursor for the research of steroidal drugs and their intermediates, with certain research value and market significance.