26 results about "Gibberella acuminata" patented technology

The present invention relates to methods for controlling or preventing infestation of a rice plant by the phytopathogenic microorganism Gibberella fujikuroi, comprising applying to a crop of rice plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The present invention discloses a seed endophyte for preventing and treating wheat fungal diseases and its application, belonging to the technical field of crop planting and crop disease biological control. The wheat endophyte (Pseudescherichia vulneris) GSE25 in the present invention was deposited in the China General Microbiological Culture Collection Center (CGMCC) on November 21, 2022, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.26158. The GSE25 strain has the ability to significantly reduce the pathogenicity of gibberellins and stripe rust on wheat plants, and can significantly reduce the mycotoxins produced by pathogens on wheat grains, has a promoting effect on crop growth and development, and has a broad-spectrum effect. The GSE25 strain of the present invention is of great value in the healthy growth, yield increase, control of pathogenic fungi and mycotoxin pollution of crops.

The utility model relates to the technical field of gibberella fermentation, in particular to a waste residue treatment device for gibberella fermentation, which comprises a support table, an outer cylinder is mounted at the top of the support table in a penetrating manner, a cover plate is placed at the top of the outer cylinder, a support frame is connected to the center of the top of the cover plate, and a variable frequency motor is mounted on the support frame in a penetrating manner. The output end of the variable frequency motor and the interior of the outer cylinder are jointly connected with a processing part. According to the utility model, the variable frequency motor runs slowly, and meanwhile, the telescopic end of the electric telescopic rod II extends out to further open the electromagnetic valve on the discharge pipe; when the telescopic end of the second electric telescopic rod corresponds to the arc-shaped notch, the follow-up variable frequency motor runs at a high speed, so that liquid in the gibberella fermentation waste residues is thrown out and discharged from the discharging pipe. The gibberellic acid fermentation waste residues are crushed by the main heating crushing rod and the auxiliary heating crushing rod, and the gibberellic acid fermentation waste residues are dried by the heating column, the main heating crushing rod and the auxiliary heating crushing rod.

The present application relates to the field of microorganism, discloses a kind of fusarium fujikuroi, the fungicide containing the bacteria and their application and the method for producing gibberellin.The preservation number of the fusarium fujikuroi is CCTCC NO:M 20221848.The present application further provides the application of the fusarium fujikuroi in producing gibberellin.The method for producing gibberellin includes the following steps: the fusarium fujikuroi and / or the fungicide is inoculated into seed culture medium to carry out seed culture and obtain seed liquid;The seed liquid is inoculated into fermentation culture medium to carry out fermentation culture.The fusarium fujikuroi provided by the present application can resist gamma ray irradiation, has good environmental adaptability and gibberellin production potential, and is suitable for industrial production.

The invention relates to methods for controlling or preventing infestation of a plant by the phytopathogenic microorganism Phyllachora maydis, comprising applying to the phytopathogen, to the locus of the phytopathogen, or to a plant susceptible to attack by the phytopathogen, or to a propagation material thereof, a fungicidally effective amount of a compound as defined in claim 1.

The invention relates to the field of gene engineering, and discloses a CRISPRi (clustered regularly interspaced short palindromic repeats i) system, a recombinant strain containing the CRISPRi system, a construction method and application of the recombinant strain, and a method for producing gibberellin. The CRISPRi system comprises a coding gene of a dCas9 mutant protein without cleavage activity, sgRNA and an induction regulation component, wherein the induction regulation component is used for regulating the expression of the dCas9 mutant protein coding gene; the sgRNA guides the dCas9 mutant protein to be combined to a target gene, so that the expression of the target gene is inhibited; the dCas9 mutant protein is obtained by mutation on the basis of a Cas9 protein, and mutation sites are D10A and H840A of the dCas9 protein; the target genes comprise squalene synthase encoding genes, fatty acid synthase encoding genes and the like, the CRISPRi system can accurately and efficiently regulate and control gibberellin gene expression, the gibberellin yield is remarkably increased, the influence on strain growth is small, regulation and control are reversible, specificity is high, and the CRISPRi system is matched with gibberellin and has good industrial prospects.

The application discloses a recombinant Gibberella fujikuroi for blocking competitive metabolic branch to improve gibberellin yield and a construction method thereof, and belongs to the technical field of industrial microorganisms.The recombinant Gibberella fujikuroi provided by the application knocks out a p450-3 gene, the GA4 yield of the recombinant strain is increased to 718.6 mg / L from 585.9 mg / L of the original strain, is increased by 22.6%, and the growth characteristics of the strain are not significantly affected.Through blocking the 13-hydroxylation competitive branch, the application realizes the redirection of metabolic flow to non-13-hydroxylation product GA4 / 7, provides an efficient chassis strain and a construction method for directional production of gibberellin components, and has important industrial application value.

The invention provides genetic engineering construction and application of a gibberellin GA4 + 7 efficient production strain. According to the method, the speed limiting step of a metabolic pathway of gibberella zeylanica is explored, key genes fpps, gs2, cps / ks and thmgr of the gibberella zeylanica are subjected to editing expression, the screened key genes ggs2, cps / ks and thmgr are subjected to co-expression, screening markers are recycled by using an inducible recombinase-based gibberella zeylanica screening marker recycling system, and finally, the strain OE: thmgr-gs2-cps / ks with the highest yield is obtained. The yield of GA4 + 7 in an optimized fermentation culture medium reaches 3.6 g / L.

The application provides application of a ppt1 gene in high-yield gibberellins GA4 and GA7. The method of the application is that an expression frame of the ppt1 is expressed in filamentous fungi by means of constructing an expression plasmid. By overexpressing the important gene ppt1 in the lysine synthesis pathway in filamentous fungi, the optimized strain only produces the intermediate metabolite GA4 and GA7, and does not produce the end metabolite GA3. The method is simple in operation and remarkable in effect, and provides a new way for construction of the high-yield GA4 and GA7 engineering strain. Compared with the wild type Gibberella fujikuroi, the Gibberella fujikuroi engineering strain provided by the application can more efficiently produce the fermentation products of GA4 and GA7, so that the GA 4+7 The mixture yield is increased by 36 times.

The invention relates to the field of gene engineering, and discloses a CRISPR-dCas9 system, a recombinant strain containing the CRISPR-dCas9 system, application of the CRISPR-dCas9 system and a gene editing method, and the CRISPR-dCas9 system comprises 5SrRNA, sgRNA, an encoding gene of adenine deamination mutant enzyme, a Linker sequence I and an encoding gene of dCas9 protein in the direction from upstream to downstream; wherein the 3'end of the adenine deamination mutant enzyme coding gene is connected with the 5 'end of the dCas9 protein coding gene through a Linker sequence I, and the sgRNA and the 5SrRNA are respectively an endogenous guide sequence and an endogenous promoter of gibberella fujiangjensis; the adenine deamination mutant enzyme is obtained by mutation on the basis of adenine deamination enzyme, and mutation sites are T111R and D119N of the adenine deamination enzyme; the nucleotide sequence of the Linker sequence I is as shown in any one of SEQ ID NO. 5, SEQ ID NO. 39 and SEQ ID NO. 40. The CRISPR-dCas9 system provided by the invention is suitable for gibberella zeylanica, provides an efficient tool for gene function research and genetic modification of gibberella zeylanica, and has relatively high industrial potential.

The invention relates to the technical field of microorganisms and application thereof, and discloses a piggyBac transposon system, a recombinant vector, a gene editing method, a recombinant strain and application of the piggyBac transposon system, the piggyBac transposon system contains piggyBac transposase, an inverted terminal repeat sequence, a target load gene sequence and a selective marker gene, the vector gene is a functional marker gene and / or a reporter gene, and the expression of the transposase is controlled through a promoter PtrpC; wherein the amino acid sequence of the piggyBac transposase is as shown in SEQ ID NO. 1; and the piggyBac transposase system also contains a vector plasmid. The gene editing method of the piggyBac transposon system can be suitable for efficiently and accurately inserting exogenous genes into gibberella zeylanica, traceless and residue-free genome modification is realized, the problem of exogenous gene residue is avoided, and the method has relatively good industrial value.

The invention belongs to the field of industrial microorganisms, and particularly relates to gibberella fujikubin and application and a fermentation product thereof. The preservation number of the gibberella zeylanica is CCTCC NO: M 20251933, the gibberella zeylanica is used for fermentation production of terpene derivatives, and the terpene derivatives can be used for preparation of plant growth regulators. The technology provides important lead compounds and technical platforms for development of green plant growth regulators, along with deep popularization of green agriculture and sustainable development concepts, the plant growth regulators with natural sources have more and more important application values, and the technology has wide industrial application prospects.

The invention discloses a dimethoxy xanthone compound and application thereof, and belongs to the technical field of agriculture. The invention finds that the dimethoxy xanthone compound shown in the formula (1) has a strong inhibition effect on phytopathogens, especially on pyricularia oryzae, wheat gibberella and phytophthora; the compound 1 can be used for bactericides, has a very high pesticide research value, and has a wide application prospect in the field of agricultural pharmacology.

The invention provides a high-yield gibberellin GA4 + 7 genetically engineered bacterium and a construction method and application thereof.The genetically engineered bacterium is constructed with gibberellic acid as a chassis, and the construction method comprises the steps that P450-2 and CPR genes are inserted into a bik2 site through a CRISPR-Cas9 gene editing technology, and carbon flux flows to synthesis of GA4 + 7; the Hmgs, Erg10 and Ggs2 genes are subjected to combined overexpression; and superposing and over-expressing endogenous Fpps and Cps / Ks genes. Compared with an original strain, the modified genetic engineering strain can better utilize starch hydrolysate as a carbon source to synthesize gibberellin GA4 + 7, the GA4 + 7 production titer is improved to 4.49 g / L after shake flask fermentation for 168 hours and is improved by 68.80% compared with the original strain, and the modified genetic engineering strain has important industrial application value.

The application discloses application of a transmembrane transporter NPF or SWEET in improving gibberellin yield of Fusarium fujikuroi, and the application comprises the following steps: heterologously expressing an exogenous transmembrane transporter NPF or SWEET in wild-type Fusarium fujikuroi by constructing an integrative expression plasmid, and then improving the gibberellin GA 4+7 yield. The application provides a new idea for improving the gibberellin yield, effectively improves the GA 4+7 yield by heterologously overexpressing the transmembrane transporter NPF or SWEET, and obtains more efficient, more stable, safer and more economically valuable gibberellin, and has a positive promoting effect on industrial production.

The application provides a naringin derivative, a preparation method of the naringin derivative, and application of the naringin derivative in preparation of medicines for preventing and treating wheat scab, tobacco black stem disease, phomopsis stem rot and pepper wilt, and has good bacteriostatic activity on wheat scab fungus, tobacco black stem fungus, phomopsis stem rot fungus and pepper wilt fungus, has a good prevention and treatment effect on plant pathogenic fungal diseases such as wheat scab, tobacco black stem disease, phomopsis stem rot and pepper wilt, and has a broad application prospect and development potential.

The invention provides an engineering bacterium for high-yield gibberellic acid GA3 as well as a construction method and application thereof, and belongs to the technical field of gene engineering. According to the invention, gibberella fujikouensis QJ47-9 is used as an original strain, and a DES gene overexpressed strain marked as a DESOE strain is obtained by overexpressing and amplifying a gene DES encoding DES dehydrogenase. On the basis of the DESOE strain, the P450-3 monooxygenase is subjected to key amino acid mutation, the selected mutation sites are F97Y, T220A, V236I and D350Q, and the mutant strain is marked as DESOE / p450-3 delta. After fermentation culture is carried out for 7 days at the temperature of 28 DEG C and the speed of 250 rpm, the yield of GA3 is increased to 2.51 g / L from 1.53 g / L of an initial strain, and the increasing rate is 64.05%; meanwhile, the yield of a byproduct GA1 is reduced to 0.06 g / L.

This invention discloses a class of acetylhydrazine derivatives with phenyl heteroatom substituents, their preparation methods, and applications; the acetylhydrazine derivatives have the general structural formula shown below, and exhibit excellent broad-spectrum antifungal effects against fungi including *Fusarium graminearum*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, *Rhizoctonia solani*, *Alternaria alternata*, *Curvularia lunata*, *Phytophthora capsici*, *Sclerotium sclerotiorum*, and *Fusarium graminearum*.

The application belongs to the technical field of microorganisms, and specifically discloses a kind of Microbacterium arborescens QHHQ09-2, a microbial agent thereof and application. The Microbacterium arborescens QHHQ09-2 of the application has a preservation number of CGMCC No.33708. The Microbacterium arborescens has the functions of IAA production, nitrogen fixation and alkali tolerance, can promote plant growth and development, slow down the decomposition or transfer of chlorophyll in plant leaves, delay plant senescence, and effectively inhibit Gibberella zeae.

The invention discloses wheat gibberella and application thereof, and belongs to the technical field of biology. A 26SrDNA (deoxyribonucleic acid) of the gene has a gene sequence as shown in SEQ ID No. 1; the gibberella zearalenone obtained through separation, screening and mutation can be efficiently synthesized into the gibberella zearalenone through liquid fermentation and solid fermentation, the highest yields of the gibberella zearalenone reach 11.5 g / L and 8.3 g / kg respectively, the production efficiency of the gibberella zearalenone is remarkably improved, and a foundation is laid for application of the gibberella zearalenone as a medicine raw material.

The invention relates to the technical field of prevention and control of gibberellic disease, in particular to a lysozyme compound preparation for targeted degradation of DON toxin and application of the lysozyme compound preparation in prevention and control of wheat gibberellic disease. The lysozyme compound preparation for targeted degradation of DON toxin is prepared from the following raw materials in parts by weight: 10-30 parts of a first enzyme source, 3-10 parts of a second enzyme source, 2-8 parts of carboxymethyl chitosan, 1-4 parts of an auxiliary agent and 1-3 parts of trehalose, the lysozyme compound preparation for targeted degradation of DON toxin prepared by the invention not only synchronously inhibits growth of gibberella and degrades DON toxin, but also improves the adhesion and infiltration capacity on the surface of wheat ears, the lasting period of pesticide effect reaches 21 days due to embedding of carboxymethyl chitosan, the DON residue in grains is less than 0.5 ppm, the European Union standard is met, the thousand seed weight and the yield increase per mu are improved, and the application prospect is wide. And the quality is effectively ensured.

The invention provides a high-yield gibberellic acid GA4 + 7 engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of gene engineering. According to the invention, gibberella zeylanica is taken as an original strain, and a P450-3 gene is knocked out by utilizing a CRISPR-Cas9 gene editing technology, so that a strain which does not generate GA1 and GA3 is obtained, and the total yield of GA4 + 7 is greatly increased; according to the present invention, the DES dehydrogenase is subjected to key amino acid mutation, the selected mutation sites are T50A, G108P and E126K, the combining ability of the mutated DES dehydrogenase and GA4 is enhanced, and the mutated DES dehydrogenase has strong catalytic ability under high temperature and acidic fermentation conditions so as to adjust the ratio of GA4 + 7 in the fermentation broth, such that the total amount of GA4 + 7 is increased by 314.95%;

The invention belongs to the technical field of microorganisms, and particularly discloses a microbacterium arborescens QHHQ09-2 as well as a microbial agent and application of the microbacterium arborescens QHHQ09-2. The preservation number of the microbacterium arborescens QHHQ09-2 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.33708, and the preservation number of the microbacterium arborescens QHHQ09-2 disclosed by the invention is CGMCC No.33708. The microbacterium arborescens has the functions of IAA production, nitrogen fixation and alkali resistance, can promote plant growth and development, can slow down decomposition or transfer of chlorophyll of plant leaves and delay plant senescence, and also can effectively inhibit Gibberella maydis.

The invention provides a gibberellin GA4 + 7 producing strain as well as a construction method and application thereof. Through a plurality of genetic engineering strategies, the method comprises the following steps: (1) heterologous expression of a plant source gibberellin transporter; (2) overexpressing an endogenous post-translational modification enzyme ppt1 to indirectly trigger mRNA degradation of a downstream key enzyme gene P450-3; and a plurality of strategies are synergistically combined, so that the industrial gibberella zeylanica mainly producing GA3 is successfully transformed into a specific engineering strain with high yield of GA4 + 7, and the generation of GA3 is completely eliminated. In combination with fermentation process optimization, the maximum yield of GA4 + 7 of the engineering strain on a shake flask level can reach 3.53 g / L, which is improved by 130 times or more compared with that of an original strain, and the engineering strain has excellent genetic stability and huge industrial application potential.

To provide a new agent for controlling soybean black root rot fungus and to provide a Calonectria ilicicola strain for use in the soybean black root rot fungus control agent.SOLUTION: An agent for controlling soybean black root rot fungus according to one embodiment contains an attenuated Calonectria ilicicola as an active ingredient. A Calonectria ilicicola according to one embodiment is a Calonectria ilicicola strain UH2-1A having the accession number NITE-AP-04095 (deposition number: NITE P-04095).SELECTED DRAWING: None