63 results about "Gibberella acuminata" patented technology

Belonging to the field of biotechnologies, the invention relates to a strain of Gibberella intermedia CA3-1 for high-efficiency conversion of dehydroepiandrosterone (DHEA) and its application. The strain is preserved in China General microbiological culture collection center and has a preservation number of CGMCCNo.4903. The Gibberella intermedia CA3-1 can convert DHEA into 3beta, 7alpha, 15alpha-trihydroxyandrost-5-ene-17-one. Under the condition of a substrate feeding amount of 5.0g/L, the substrate conversion rate can reach about 90%, and the product yield can be about 80%, so that the strain provided in the invention is of great significance for steroid drug production by bioconversion.

The present invention provides one new gene MgKIN17 from Pyricularia gisea, and the gene features that it has the amino acid sequence from the site 1 to the site 3544 in the SEQ ID No. 1, the cDNA with the nucleotide sequence of SEQ ID No. 2, the promoter with the nucleotide sequence of SEQ ID No. 4, and coded protein with the amino acid sequence of SEQ ID No. 3. The insertion mutation and gene complementation of the present invention shows that the destruction of the gene leads the formation of Pyricularia gisea infecting organ and reduced lesion capacity of rice. Wheat bakanae fungus, corn stalk rot and other pathogenic fungus have homogenous protein with homogeneity over 40 % to MgKIN17 and maybe similar biological function. The gene of the present invention and its protein expression and modification may be used as the target site for designing and screening antifungal medicines.

The invention discloses a dihydroagarofuran type sesquiterpene compound, the preparing method of the compound, and the application of the compound in preparing anti-microbial pesticides and relates to the field of medicinal chemistry. According to the application of the novel sesquiterpene compound in anti-microbial pesticides, the structure of the adopted sesquiterpene compound is shown in the figure of the abstract and is named 1beta-acetyl-6alpha-(5-carboxyl-N-methyl-2-pyridone)-8beta-nicotinoyl-9alpha-benzoyl-beta-dihydroagarofuran. The compound is characterized in that the stem leaves of the plant Monimopetalum Chinese Rehd. are used as raw materials, and then the compound is obtained through extraction and separation with the natural product chemistry method. As a pesticide, the compound has a remarkable resisting effect on pests including plutella xylostella, cabbage caterpillar, corn armyworm, prodenia litura, argyrogramma agnate, pea aphids, rice-stem borer, rice tryporyza incertulas and henosepilachna vigintioctomaculata. As a sterilant, the compound has a remarkable resisting effect on sclerotinia sclerotioru, rhizoctonia cereal, fusarium graminearum and botrytis cinerea person.

The invention discloses 2,8-bis(trifluoromethyl)quinoline 4-modified derivatives, a preparation method thereof and an application of the derivatives in prevention and treatment of plant diseases. Thetest results show that the compounds have excellent control effect on sclerotiniasclerotiorum, rhizoctonia solani, wheat phytoalexin, pyriculariaoryzae, phyllostictazeae, botrytis cinerea, watermelongummy stem blight and cotton fusarium wilt. The derivatives of the invention are simple in preparation process, low in price and easy to obtain raw materials, high in product purity and wide in bactericidal spectrum, and are expected to be developed into a new bactericide.

The invention discloses a rice endotrophic azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on fusarium graminearum and a purpose thereof. The rice endotrophic azotobacter is Burkholderia sp. GDSD125 and has the preservation number being CGMCC No.5038 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD125 CGMCC No.5038 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.

The invention discloses a Yangbi walnut germin-like protein gene JsGLP1 and an application thereof. The nucleotide sequence of the JsGLP1 gene is as shown in SEQ ID NO:1, and is capable of encoding germin-like protein. Through functional genomics related technological research, the gene JsGLP1 is proved to have the function of enhancing plant fungal infection resistance; the antifungal gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for over expression; the transgenic tobacco plant is strong in the in-vitro antifungal activity; and JsGLP1 over-expression transgenic tobacco has a significant inhibitory effect on the growth of sclerotinia sclerotiorum, beaded gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

The invention discloses a pathogenesis-related protein 1 family gene PnPR1-2 of panax notoginseng. The nucleotide sequence of the pathogenesis-related protein 1 family gene PnPR1-2 is as shown in SEQ ID No. 1. The pathogenesis-related protein 1 family gene PnPR1-2 comprises protein encoded with an amino acid sequence as shown in SEQ ID No. 2. Related technological researches of functional genomics prove that the PnPR1-2 gene is capable of increasing the fungus resistance of plants; when the PnPR1-2 gene is built onto a plant expression carrier and transferred into tobacco for overexpression, the transgenic tobacco plant is high in in-vitro antifungal activity, and the overexpression PnPR1-2 transgenic tobacco has an evident inhibiting effect on the growth of various fungi such as Fusarium solani, Verticillium Fusarium, beaded gibberella and Alternaria panax.

The invention discloses application of a Yangbi big-bubble walnut transcription factor gene JsWRKY1. The JsWRKY1 gene is proved to have a function of enhancing the fungal disease resistance of a plant through technical research related to functional genomics. The antifungal gene JsWRKY1 is established to a plant expression vector and transferred into a tobacco for overexpression, and a transgenic tobacco plant has very high in-vitro antifungal activity, and a transgenic tobacco subjected to JsWRKY1 overexpression achieves obvious inhibiting effect on the growth of botryosphaeria dothidea, moniliform gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

The invention belongs to the application field of biology engineering technology, and particularly relates to a method of immobilizating gibberella CA3-1(Gibberella intermedia) with a preserved number of CGMCC No.4903 and using the gibberella for biological transformation to prepare nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid includes: immobilizing the gibberella CA3-1, adding a proper amount of 3-cyanopyridine, and choosing an optimum reaction condition for immobilizing thalluses to obtain a product which is the nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid strengthens tolerance of the thalluses to substrates after the thalluses are immobilized, improves production capacity of unit thalluses, simplifies separation processes of products greatly, increases reutilization times obviously, and provides a foundation for achieving technology of performing a biological transformation method by using the gibberella to prepare the nicotinic acid.

The invention relates to an application of a lilium regale germin protein gene LrGLP1. The nucleotide sequence of the LrGLP1gene is shown as SEQ ID NO:1, a germin-like protein is encoded, and according to the invention, functional genomics related technical researches prove that the LrGLP1gene has a function of improving the fungal infection resistance of plants. An antifungal LrGLP1 gene in the invention is built into a plant expression vector, and transferred into a tobacco to be subjected to excessive expression, so that a transgenic tobacco plant has an extremely strong in-vitro antifungal activity. LrGLP1overexpressed transgenic tobaccos have an obvious inhibitory effect on the growth of alternaria alternate, sclerotinia sclerotiorum and catenuliform gibberella.

The invention discloses a method for identifying wheat scab extension resistance through wheat ear top double-flower inoculation. A prepared gibberella spore liquid is inoculated into bilateral florets of a sixth spikelet at the top of a wheat ear by utilizing a double-flower instilling method in a wheat flowering stage, a browning condition of inoculated spikelet is recorded, a diseased spikeletrate of infected spikelet at the lower part of the inoculated spikelet after inoculation is counted, and wheat scab extension resistance is dynamically evaluated. According to the inoculation method,a diseased part mainly expands downwards from the sixth spikelet on the top, the diseased spikelet rate at the lower part of the sixth spikelet is counted, diseased parts are consistent, the problem that identification reliability is affected due to the fact that white spikes are prone to being withered through a traditional single-flower instillation method is solved, and the extension resistanceof varieties can be reflected more accurately.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats)/Cpf1 gene editing system as well as a construction method and the application thereof in gibberella. The system is a skeleton plasmid pCpf1Ff-DR, and comprises an Fncpf1 gene, a functional crRNA gene, a promoter pGPD, a promoter pTRPC, a strong RNA polymerase III type promoter 5srRNA and a hygromycin selection marker hph. The system is high in efficiency, strong in universality, easy to operate and capable of being rapidly applied to filamentous fungus gibberella, and the gene editing system can be used for achieving knockout of the bicarpin gene cluster as long as 17kb in the gibberella. According to the method, large-fragment gene knockout is achieved in gibberellin for the first time, it is found for the first time that knockout of the bicarpin gene cluster is beneficial to increase of the yield of gibberellin, and the method is expected to be further used for transforming gibberellin industrial bacteria, shortening the time of bacterial strain upgrading and providing powerful guarantee for increasing the yield of gibberellin and increasing economic benefits.

The invention discloses a method for identifying wheat scab resistance. The method comprises the following steps: inoculating wheat spike base internodes with a gibberella spore liquid after wheat heading, recording the browning condition of an inoculation point, judging whether the inoculation succeeds or not, respectively counting the scab rate on the 7th day, the 14th day and the 21st day afterthe inoculation, and dynamically evaluating the wheat scab resistance. The method has the greatest advantages of fastness and accuracy in observation of the disease condition and identification of the variety resistance, and solves the problems of difficult control of the spore inoculation amount, slow disease incidence, large simultaneous inoculation of the same variety in spike rate difference,high stem corrosion possibility and identification reliability influence of a stem injection method. Compared with the ear base stalk injection method, the method of the invention has the advantagesof consistent and small dosage of the spore liquid at the inoculation part, improvement of the consistency of disease attack, high inoculation efficiency, low cost, obvious phenotypic difference between variety resistance and variety susceptibility, and facilitation of common technicians can judge the gibberella disease resistance of wheat varieties.

The invention discloses Julans sigillata Dode proline-enriched protein gene JsPRP1 and applications thereof. The nucleotide sequence of the JsPRP1 is represented by the SEQ ID No.1, and the encoding is rich in proline protein. Through the related technologies and researches of functional genomics, the results show that JsPRP1 gene has a function that can improve the plants' performance on resisting fungal infection. The anti-fungus gene JsPRP1 can be built on an expression carrier and over-expressed in tobacco, and the transgene tobacco plants has a strong in-vitro activity on resisting fungi. The transgene tobacco, where the JsPRP1 gene is over-expressed, has a prominent inhibiting effect on the growth of colletotrichum gloesporioides, sclerotinia sclerotiorum, botryosphaeria, and catenulate gibberella.

The invention provides a fullerene/gutta-percha ultraviolet absorption film material and a preparation method and application thereof, and belongs to the technical field of composite materials. The fullerene/gutta-percha ultraviolet absorption film material provided by the invention comprises a film matrix and fullerene dispersed in the film matrix, wherein the film matrix is formed by gutta-percha, and the fullerene is C60. The film material provided by the invention is obtained by compounding fullerene C60 and gutta-percha, wherein the fullerene C60 has good ultraviolet absorption capacity,and the gutta-percha has good biodegradability and is environment-friendly, so that the film material obtained by compounding the fullerene C60 and the gutta-percha retains respective characteristics,is good in biodegradability and has a wide ultraviolet absorption band, the ultraviolet absorptivity in the wave band of 200-400 nm almost reaches 100%, and a good prevention and control effect on the gibberella saubinetii is achieved.

The invention relates to a genetically engineered bacterium for producing gibberellin GA3 at high yield, a construction method and application. According to the present invention, by enhancing the gene expressing the nitrogen source regulatory factor AreA and the gene expressing the global regulatory factor Lae1 in the gibberella zeylanica, the positive regulation effect of the gibberella zeylanica on the related gene in the gibberellin anabolism path is enhanced, the transcriptional level of the related gene is improved, and the accumulation of gibberellin GA3 is improved. According to the recombinant gibberellin GA3 high-yield recombinant gibberellin GA3 provided by the invention, compared with wild gibberellin GA3, the transformed recombinant gibberellin GA3 has the advantages that the overall transcriptional level of a GA gene cluster is enhanced, the synthesis capability of a metabolic process is improved, the transcriptional inhibition of GA3 synthesis related genes is weakened, a carbon source substance and a nitrogen source substance can be better utilized for producing gibberellin GA3, and the yield of gibberellin GA3 is improved. The level of the transformed strain with the optimal performance for producing gibberellin GA3 through fermentation is improved from 2 g/L to 3.25 g/L compared with the original strain, the production capacity of gibberellin GA3 is remarkably improved, and the strain can be applied to large-scale production of gibberellin GA3.

The invention discloses gibberella sp. QJP2 and application of the gibberella sp. QJP2 in weeding. The Gibberella intermedia QJP2 is separated from suaeda glauca rhizosphere soil collected in the wetland of the intertidal zone of the Jiaozhou Bay in Qingdao, and is preserved in the China General Microbiological Culture Collection Center on December 9, 2016, and the preservation number is CGMCC No.13199. The Gibberella intermedia QJP2 has the advantages that the Gibberella intermedia QJP2 can be used for preparing the Gibberella intermedia QJP2; a fermentation product of the strain has relatively good inhibitory activity on malignant weed barnyard grass in a paddy field, and is safe to use for rice. When the concentration of a fermentation product is 100 mu g/mL, the growth inhibition rate of the fermentation product to radicles of barnyard grass seedlings is 81.3%, and the growth of radicles of rice seedlings is not affected. Therefore, the suaeda salsa sourced gibberellic fungus QJP2 has the potential of being developed into a biological herbicide for preventing and removing barnyard grass.