New Kosteletzkya virginica (L.) Presl. sesquiterpene compound, and preparation method and use thereof

A seaside malvain and compound technology, applied in the field of new sesquiterpene compounds and their preparation, can solve the problems of economic loss, chlorosis and yellowing of cotton leaves, wilting and shedding and the like

Active Publication Date: 2013-10-16
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Verticillium wilt is a soil-borne fungal vascular disease that infects through the roots. After infection, it will cause cotton leaves to turn green and yell

Method used

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  • New Kosteletzkya virginica (L.) Presl. sesquiterpene compound, and preparation method and use thereof
  • New Kosteletzkya virginica (L.) Presl. sesquiterpene compound, and preparation method and use thereof
  • New Kosteletzkya virginica (L.) Presl. sesquiterpene compound, and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Cut 13 kg of seaside mallow fresh tubers (water content is 75%) into 5 cm sections, add 95% ethanol 6 times the volume of the crude drug, reflux extraction twice, each time for 2 hours, combine the crude extracts, and reduce pressure at 70 ° C Concentrate to 0.5 times the volume of the medicinal material, then add ethyl acetate to extract 5 times at a volume of 1:2, combine the ethyl acetate extracts, concentrate under reduced pressure, and dry to obtain 0.065 kg of ethyl acetate extract. The ethyl acetate extract was segmented by silica gel column chromatography, and petroleum ether, petroleum ether-ethyl acetate (30:1), petroleum ether-ethyl acetate (10:1), petroleum ether-ethyl acetate ( 4:1), petroleum ether-ethyl acetate (1:1) solution elution, the eluents with similar components were combined and concentrated to obtain 6 fractions of Fr.-A to Fr.-F. Using methods such as silica gel column chromatography, gel LH-20 column chromatography, and recrystallization, the ...

Embodiment 2

[0026] Cut 5 kg of seaside mallow fresh branches (water content is 80%) into 10 cm sections, add 5 times the crude drug volume of methanol, reflux extraction for 3 times, each time for 1.5 h, combine the crude extracts, and concentrate under reduced pressure at 50 °C to 1 times the volume of the medicinal material, and then added ethyl acetate at a volume of 1:1 for extraction three times, combined the ethyl acetate extracts, concentrated under reduced pressure, and obtained 9 grams of ethyl acetate extract of seaside mallow. Using methods such as silica gel column chromatography, gel LH-20 column chromatography, and recrystallization, the compound virginicin (400mg) was isolated from the ethyl acetate extract with a yield of 0.01%, and the purity of the product detected by HPLC was 98.8%. %.

Embodiment 3

[0028] Cut 1 kg of seaside mallow dry branches and leaves into 3 cm sections, add 6 times the crude drug volume of 80% methanol / water, reflux extraction twice, each time for 2 hours, combine the crude extracts, concentrate under reduced pressure at 50 ° C to 1.5 times the medicinal material Measure the volume, then add ethyl acetate to extract 5 times according to the volume of 1:2, combine the ethyl acetate extracts, and concentrate under reduced pressure to obtain ethyl acetate extracts. Using methods such as silica gel column chromatography, gel LH-20 column chromatography, and recrystallization, the compound virginicin (80 mg) was isolated from the ethyl acetate extract, and the yield was 0.008%. The purity of the product detected by HPLC was 98.4%. %.

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PUM

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Abstract

The invention discloses a new Kosteletzkya virginica (L.) Presl. sesquiterpene compound, and a preparation method and a use thereof, and belongs to the chemical field of natural products. The above new cadinane type sesquiterpene compound is obtained by extracting and separating from a raw material the rhizome of a halophyte Kosteletzkya virginica (L.) Presl. through chemical processes of the natural products. The structural formula of the compound is shown in the specification, is named as virginicin, has a strong killing effect on Verticillium dahliae, and can be used for controlling cotton verticillium wilt.

Description

1. Technical field [0001] The invention discloses a new sesquiterpene compound, its preparation method and application, and belongs to the field of natural product chemistry. It is characterized in that it is a new juniperane-type sesquiterpene compound obtained by extracting and separating the rhizome of the halophyte seaside mallow (Kosteletzkya virginica (L.) Presl.) through natural product chemical methods, named as Virginicin, the compound has a strong killing effect on Verticillium dahliae, and can be used for the prevention and treatment of Verticillium dahliae. 2. Background technology [0002] Cotton is an important economic crop in the world, and China is a big cotton production, consumption and textile country. my country's annual cotton planting area is 70-80 million mu, with a total output of about 6 million tons, accounting for a quarter of the world's total output. After cotton verticillium wilt was first discovered in the United States in 1914, it quickly s...

Claims

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Application Information

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IPC IPC(8): C07C47/575C07C45/78A01N35/04A01P3/00
Inventor 陈雨冯煦赵友谊王鸣王奇志单宇管福琴印敏孙浩
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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