326results about How to "High decolorization rate" patented technology

The present invention is an industrial waste water microwave electrodeless ultraviolet photocatalysis-dual membrane separation coupling treatment device, the device mainly consists of a reactor (1), a membrane separation system (2), a microwave electrodeless ultraviolet light source system (4), an aeration system, and an ozone tail gas decomposition device (7) connected to the reactor, and an inlet and outlet water system, wherein: the upper and lower parts of the reactor are respectively the reaction zone and the aeration zone, which are separated by a water distribution plate (5); the membrane separation system The microwave electrodeless ultraviolet light source system is located in the reaction zone and is separated by a corrugated partition (3); the aeration system is composed of a microporous aeration head (6) and a blower (8), and the microporous aeration head is located in the aeration At the bottom of the zone, the blower sends air to the aeration zone through the air duct. The invention has the characteristics of high reaction rate, complete degradation of organic matter, long-term operation and the like, and has strong operability and high safety. It is suitable for the treatment of refractory organic industrial wastewater, and it is also suitable for sterilization and disinfection in the field of water supply.

The invention belongs to the chemical field of natural products and particularly relates to a preparation method and use of moringa oleifera leaf polysaccharides. The preparation method comprises the steps of carrying out combined microwave-ultrasound extraction, hydrochloric acid method deproteinization, hydrogen peroxide method decoloration and AB-8 macroporous adsorption resin column separation, so as to prepare the moringa oleifera leaf polysaccharides. The preparation method of the moringa oleifera leaf polysaccharides has the advantages of short extraction time, low extraction temperature, low energy consumption and high extraction rate and purity. Besides, an animal experiment proves that the prepared moringa oleifera leaf polysaccharides have remarkable treatment effect to hyperuricosuria; by decreasing the content of cholesterol, triacylglycerol, urea nitrogen, creatinine and xanthine oxidase, the production of uric acid is achieved; meanwhile, by directly decomposing uric acid, renal functions are improved, the excretion of uric acid is promoted, and blood vessels are protected, thereby being favorable to the recovery of patients with the hyperuricosuria. The preparation method has a wide medical application prospect.

The invention discloses a method for treating high-concentration water-based ink waste liquid and dehydrating sludge in the high-concentration water-based ink waste liquid. The method is characterized by comprising the following steps: collecting the water-based ink waste liquid, pumping the collected water-based ink waste liquid into a reactor, then adding inorganic acid into the waste liquid, carrying out stirring and adjusting the pH value of the waste liquid until the waste liquid is acidic; allowing the sludge to enter and be located on a dehydration filter belt, heating the sludge with microwaves, carrying out squeezing and dehydration, and spraying water on the dehydrated sludge to rapidly cool the surface of the dehydrated sludge; and taking the sludge off the filter belt and discharging dehydration water into a subsequent waste water treating system. With the method provided by the invention, integrated removal of pollutants in the ink waste liquid and dehydration of the sludge is realized; the removal rate of the pollutants reaches more than 90%; a decolorization ratio is up to more than 99%; and the treated sludge has a water content of lower than 40%.

The invention discloses a wastewater treatment process of oil-retaining emulsion liquid waste water, which adopts electrochemical demulsification. The technological process is as follows: the oil-retaining emulsion liquid waste water; grate pretreatment; adjustment of water quality and volume; electrochemical demulsification; oxidation or precipitation; filtration; clear water. The electrochemical demulsification and separation reaction pond during electrochemical demulsification has the advantages of continuous demulsification and separation, operation under normal temperature and atmospheric pressure, low operating cost, small occupied area of the complete equipment, convenient maintenance, easy operation, applicability to demulsification and separation of oil-retaining emulsion liquid waste water. The electrochemical demulsification has the advantages of integrated mode of ionization and chemical reaction, optimal demulsification effect, high oil-water separation efficiency, high decoloration efficiency, good effluent close to the standard of tap water examined by sensory organ, high water quality up to first class discharge standard and recyclability.

The invention discloses a protein flocculant for treating printing and dyeing wastewater. The protein flocculant for treating the printing and dyeing wastewater is prepared from various animal protein powders originating from cheap waste animal pelts, hoofs, animal fur powders and bean pulps aiming at different groups in a protein molecule and a chemical modifier for reaction. The invention simultaneously provides a protein flocculant compound. The invention provides a simple and practicable method; and by utilizing the method, various dye waste waters discharged in printing and dyeing plants and dye production plants can be effectively treated, and the product cost is cheap.

This invention discloses a method for preparing high-purity xylooligosaccharide from straw by using enzyme and membrane technology. The method comprises: steam-exploding straw, adding xylanase, hydrolyzing, centrifuging or filtering to obtain supernatant, performing nanofiltration or vacuum-concentration, acid-treating, centrifuging or filtering to obtain supernatant, performing active carbon adsorption, ultrafine filtration and ion exchange resin column adsorption, and concentrating or spray-drying to obtain high-purity xylooligosaccharide slurry or powder (xylooligosaccharide content is up to 70-95%). The residues of straw produced during xylooligosaccharide preparation can be used for papermaking, thus is environmentally friendly. Straw has an abundant resource, thus the cost is low. The method has such advantages as high treatment quantity, integrated operation, simple process, and high efficiency.

This invention relates to a method for electrooxidation degradation of dye waste-water by utilizing activated carbon fiber, particularly by using the activated carbon felt as anode material. It comprises steps of: (1), feeding wastewater into the electrolytic cell, (2), the cell is equipped anode activated carbon fiber and stainless steel cathode, (3), power-in the DC power and starting the agitator, proceeding electrolysis with current density of 15-35mA / cm2, voltage of 5-12V. The absorption makes the enrichment of organic dye on the surface of the activated carbon, and oxidation and degradation of the absorbed dye occurs by electrocatalysis oxidation, after the treatment, the decolourisation is upto 99%, COD removal rate is 87.9%.

The invention discloses a decolorizer for reducing an anisidine value in soybean oil for injection and a method for preparing the same. According to the mass percentage, modified attapulgite is mixed with 2 to 3 percent of carbonized bean husk ash to prepare the decolorizer; and the method comprises the following steps: 1, purifying the attapulgite; 2, activating the attapulgite; 3, modifying the attapulgite; 4, preparing the carbonized bean husk ash; and 5, according to the mass ratio of 97-98:2-3, mixing the modified attapulgite with the carbonized bean husk ash in a stirring tank, and grinding the mixture by a pulverizer to obtain the decolorizer. The decolorizer prepared by the method has the advantages of quick decolorizing speed and good decolorizing effect.

The invention discloses an air floatation lustration method of sugar juice. Nitrogen is used as foaming air supply instead of air; thin juice (such as mixed juice, diffusion juice, neutral juice, clear juice and filtered juice) and concentrated sugar juice (such as syrup and molasses) in a sugar-refinery are processed through floatation lustration. The lustration method solves the problems that oxygen generated in the air floatation lustration method which takes the air as the foaming air supply has the oxidation encolouration side effects on the phenols in the sugar juice, the intensity of sulfitation is reduced, the sugar juice is fermented under the low temperature, etc., the sugar juice with better quality is obtained, and the product quality of the sugar-refinery is increased.

The invention relates to a polyethyleneimine modified chitosan microsphere medium. The medium adopts a crosslinked chitosan microsphere with aldehyde surface, wherein high molecular weight polyethyleneimine is modified on the surface; the weight of polyethyleneimine modified on the surface is 731 plus or minus 32 mu mol / g-1,275 plus or minus 37 mu mol / g according to the ion exchange capacity; the particle size of the polyethyleneimine modified chitosan microsphere medium ranges from 50 mu m to 200 mu m; and the molecular weight of polyethyleneimine molecules ranges from 1,200 to 60,000. According to the polyethyleneimine modified chitosan microsphere medium, the ion exchange capacity of the medium is improved, and accordingly, the decolorization ratio of the medium to dye wastewater is increased; the maximum ion exchange capacity of the polyethyleneimine modified chitosan microsphere medium can be 1,275 plus or minus 37 mu mol / g and is far higher than that of unmodified chitosan microsphere medium with the ion exchange capacity of 418 plus or minus 31 mu mol / g; and the modifying method is simple in process and low in cost.

The invention discloses a printing and dyeing wastewater treatment process which comprises the following steps: adding a filter aid into wastewater, flocculating the wastewater, filtering to remove suspended matters from the wastewater, and reserving small suspended matters with colors and dissolved matters; adding a reducing decolorizer into the wastewater subjected to primary filtration for treating dyeing wastewater of direct dyes, so that dye molecules in the wastewater form a reductive degradation product; enabling the wastewater containing the reductive degradation product to pass through an adsorption material for adsorbing the reductive degradation product or other materials in the wastewater to the solid surface due to the adsorption effect; pouring a non-water-soluble organic solvent into the wastewater subjected to adsorption, and vibrating the solvent and the wastewater together, wherein the water phase is colorless when two phases are separated, and the dyes are accumulated on the upper-layer organic phase; and pouring a certain quantity of clean water into the wastewater subjected to extraction for diluting the wastewater. The process has the advantages that the pH value of the wastewater does not need to be adjusted, the effect is good, and the cost is low.

The invention discloses a synchronous decoloration and nitrogen recovery method for high-concentration printing waste liquids. The method is characterized by comprising steps as follows: cation exchange resin is added to a certain quantity of the high-concentration printing waste liquids and stirred until pasty sludge is formed through a reaction; treated wastewater is taken as back washing water to wash the resin clean; the sludge in the back washing water is precipitated and separated from wastewater, the precipitated sludge is mechanically pressed and dewatered; the washed cation exchange resin is filtered and dewatered; an acid solution for dewatered resin is treated, nitrogen is recovered, and the resin is regenerated; the regenerated cation exchange resin is added to the high-concentration printing waste liquids and recycled. With the adoption of the treatment method, the decolorization rate of the printing waste liquids is higher than 99.5%, the ammonium-nitrogen removal rate is about 90%, the CODCr removal rate is higher than 90%, synchronous decoloration and nitrogen recovery of the waste liquids are realized, and the content of acid group ions in the wastewater is not increased.

The invention discloses a ganoderma lucidum polysaccharide extracting method. The extracting method comprises the steps: firstly, smashing ganoderma lucidum sporocarp and sieving by a 80-mesh sieve; then putting into a steam explosion device to be exploded, adding into water to be soaked for 1.5h according to a solid to liquid ratio as 1 to (10 to 20) and then heating to 30 to 50 DEG C to performultrasonic treatment; adjusting a material liquid temperature, a pH value, protease and cellulase; after enzymolysis finishes, warming to denaturalize enzyme, centrifuging, decoloring resin, deproteinizing, filtering, vacuum concentrating and vacuum drying under a lower temperature to obtain high-activity and high-purity ganoderma lucidum polysaccharide solid powder. Therefore, a polysaccharide extracting rate and a polysaccharide production efficiency are improved, a space structure of polysaccharide is prevented from being damaged, biological activity of the polysaccharide is prevented frombeing reduced, and the ganoderma lucidum polysaccharide extracting method is more suitable for industrial production.

The invention discloses a strong-flavor rapeseed oil processing method, which comprises: pounding agastache rugosa, dianthus chinensis l, peppermint, melissaofficinalis l, osmanthus fragrans and camellia japonica l, adding water, decocting, and filtering to obtain an ingredient liquid; selecting high-quality rapeseed, peeling to obtain rapeseed kernel, heating for a certain time with steam, adding edible salt, uniformly mixing, adding to the ingredient liquid, soaking for a certain time, filtering, adjusting the water content of the rapeseed kernel, carrying out a microwave pretreatment, cooling, and carrying out cold pressing to obtain pressed oil and pressed cake; and carrying out degumming, alkali refining, decoloring and deodorizing on the pressed oil to obtain the strong-flavor rapeseed oil. According to the present invention, the strong-flavor rapeseed oil processing method is provided, wherein the low temperature cold pressing is used, the oil yield is high, the prepared strong-flavor rapeseed oil has advantages of rich flavor, high nutritional value, good oxidation stability and no pollution, the pure nature property of the rapeseed oil is ensured, and the requirements of green food are met.

The invention relates to a catalyst for ozone oxidation of organic matters and a preparation method of the catalyst. The catalyst is prepared by the following steps: mixing multi-metal oxide, activated carbon, bentonite, a metal oxide adhesive and an activated carbon adhesive, wherein mass ratio of the multi-metal oxide to the activated carbon to the bentonite is 1:(0.2-5): (0.2-0.5), the mass of the metal oxide adhesive added during the mixing is 0.2%-5% of total mass of the multi-metal oxide, and the mass of the activated carbon adhesive is 0.2%-5% of the total mass of the catalyst; and after the mixing, carrying out mechanical granulation, drying particles, and carrying out microwave radiation, so as to obtain the finished product. The catalyst has the beneficial effects that by utilizing an ozone oxidation process of the organic matters, the ozone oxidation efficiency of the organic matters can be improved; and when the catalyst is applied to wastewater treatment, the wastewater COD removal efficiency of ozone is generally more than 45%, meanwhile, the B / C ratio of the wastewater can be increased, the decolorization ratio of the wastewater can be increased, and the service life of the catalyst is generally more than 5 years.

The invention relates to a method for preparing mesoporous TiO2 / activated-carbon in-situ composite material, and belongs to the technology field of nanometer composite material. A natural microbial surfactant and a sugar additive are added in water, stirred, and cultured to form fermented emulsion; inorganic solution containing Ti<4+> is dropwise added in the emulsion, and the pH is adjusted to be 7 to 9; then precipitates are water-washed and alcohol-washed, and are processed through heat treatment; the precipitates are rapidly heated up in a calciner until the temperature reaches 300 to 600 DEG C and then are carbonized to obtain the mesoporous TiO2 / activated-carbon in-situ composite material. By utilizing the nanometer multilayered vesicle structure of the microorganisms and the mineralization deposition process, the preparation method synthesizes the mesoporous TiO2 / activated-carbon in-situ composite material with photoelectric double functions and high catalytic activity under the mild condition, wherein, the specific surface area is high, the catalytic activity is high, and the reproducibility of the mesoporous structure is good.

The invention relates to an environment-friendly technology for cleanly producing sodium glutamate. The environment-friendly technology comprises the following steps of: simultaneously adding liquid ammonia and sodium carbonate into sodium glutamate fermentation liquor for reaction, and thereby obtaining coarse fermentation liquor; filtering by micromembrane, and collecting degerming liquid and mycoprotein; adopting the mycoprotein to prepare probiotics, pumping the degerming liquid into a bleaching tank to perform decoloration process, and concentrating and crystallizing to obtain sodium glutamate coarse crystal; then refining and processing waste water; the technology is economical and environment-friendly, and has wide application prospects.

The invention discloses a hierarchical porous nickel-aluminum alloy membrane with the electrocatalytic performance and a preparation method thereof. According to the method, a certain proportion of nickel-aluminum powder is taken, is mixed and pressed, and then is molten into a porous alloy membrane in a sintering mode, then the interior of the porous alloy membrane is enabled to form a microporous morphology through chemical corrosion, the alloy membrane forms a nano-pore structure with high specific surface area and high catalytic activity due to the fact that nickel-aluminum alloy is corroded by sodium hydroxide, so that the membrane separation and the electrocatalysis function are integrated without adding an additional supported catalyst, and therefore the processing capacity of the alloy membrane to dye wastewater is greatly improved, and the decolorization rate of the alloy membrane at the maximum flux is increased from 55% to 95% or above.

This invention relates to decoloring and refining method of sugarcane sugar solutioní»s ion-exchange method. Pretreating the sugarcane melada and removing large-molecular weight organic to get the sugarcane liquor solution. The liquor is entered big-aperture type alkali anion-exchange resin; the pigment and ash content of the sugarcane liquor undertakes exchanging absorption by alkali anion-exchange resin. Then remove the pigment and ash content by adsorption which uses regeneration agent sodium hydroxide. The sugarcane syrup which has removed pigment and ash content enter big-aperture type weak acid anion-exchange resin. The big-aperture type weak acid anion-exchange resin can absorb magnesium and calcium in the sugarcane syrup. Then absorb it to remove magnesium and calcium by regeneration agent hydrochloric acid.

The present invention discloses a high-concentration printing ink waste liquid treatment and sludge dewatering integration method and a high-concentration printing ink waste liquid treatment and sludge dewatering integration apparatus. The apparatus comprises a printing ink waste liquid storage tank, a reaction curing dewatering device, a solid-liquid separation sieve and an acid storage tank, wherein a sewage pipe is arranged between the waste liquid storage tank and the reaction curing dewatering tank for connection and is provided with a sewage pump, a stirrer is arranged in the reaction curing dewatering tank, the motor of the stirrer adopts the stepless speed regulating, the lower portion of the stirring shaft is helical, an acid addition pipe is arranged between the acid storage tank and the reaction curing dewatering tank for connection and is provided with a valve, the bottom portion of the reaction curing dewatering tank is provided with a rapid opening valve, and the solid-liquid separation sieve is positioned just below the reaction curing dewatering tank. With the treatment method and the equipment of the present invention, the printing ink waste liquid pollutant removing and sludge dewatering integration is achieved, the waste liquid decolorization rate achieves more than or equal to 99%, the CODCr removal rate achieves more than or equal to 90%, the sludge moisture content is less than 60%, and the sludge is naturally dried without other dewatering equipment after being taken out.

The invention discloses a refined method of Chinese wax, comprising 1) acid cleaning to removal impurity, which means acidic solution is adopted to removal the phospholipids and micro metal ion in the liquated raw wax; 2) adsorbing to decolor, which means the floridin and the silicon dioxide are adopted to decolor and removal the protein in the liquated raw Chinese wax under vacuum state. The refined method of Chinese wax has the advantages of simple technique and convention operation. The appearance of the made Chinese wax and the physicochemical index can reach the first wax standard set by < trading cooperative standards of P.R.C- Chinese wax [GH011-80] >, and also has the advantages of increasing the productive rate and the purity of the Chinese wax and lowering the productivity energy consumption.

The invention relates to a decolorizing method for tea oil. The decolorizing method comprises the following steps of: weighing non-decolorized tea oil, and heating the non-decolorized tea oil under a vacuum condition; adding a certain amount of mixed decolorizing agent to the tea oil; stirring the mixture under the condition that the heating temperature is kept unchanged; cooling and centrifuging the tea oil after the tea oil is decolorized for a certain time; and separating the tea oil from the decolorizing agent to obtain a decolorized finished tea oil product. The decolorizing method disclosed by the invention have the advantages of simpleness and convenience for operation and high working efficiency; and the tea oil processed by using the method disclosed by the invention has the advantages of light color and luster and good transparency. According to the decolorizing method disclosed by the invention, quality and preservation stability of the tea oil are improved; and a good example is provided for the development of tea oil industry as well as the comprehensive utilization of resources.

The invention belongs to the field of nano photocatalysis materials, and particularly relates to a tungsten-nitrogen (W, N) binary co-doped nanometer TiO2 photocatalyst and a preparation method thereof. In the method, the (W, N) binary co-doped nanometer TiO2 photocatalyst is synthesized by using a sol-gel method and a mechanical alloying method together, wherein the doping amounts of W and N can be adjusted and controlled. The (W, N)-TiO2 photocatalyst prepared with the preparation method disclosed by the invention has high visible light absorbing performance, an absorption band edge in a visible light region can extend to 650 nanometers, and the photon efficiency of a TiO2 material can be remarkably increased. Meanwhile, the (W, N) binary co-doped nanometer TiO2 photocatalyst has superior photocatalysis performance under visible light, which is superior to that of an internally-known TiO2 commodity P25. Due to the adoption of the (W, N) binary co-doped nanometer TiO2 photocatalyst, the problems that the range of an absorption spectrum is mainly in an ultraviolet light region when WO3 is loaded onto TiO2, and the like existing in the prior art can be solved, and the nano TiO2 photocatalyst with high light absorption performance and catalytic activity is obtained under visible light. The preparation method is easy, and has industrial prospect.

The invention belongs to the chemical field, and provides a composite phosphate removing decolorant and a method for removing phosphor and decolouring, the invention solves the technical problems of phosphor removal and poor decolouring effect of the product during the waste water treatment process in the prior art, the composite phosphate removing decolorant of the present invention is polymerized by ferrous sulphate, calcium hypochlorite, NaClO3, H2O2, HPMA, borax and purified water in proportion, and the mass ratio are 1: 0.1-0.75: 0.001-0.5: 0.001-0.15: 0.01-0.28: 0.001-0.15: 0.01-55.50. The phosphor removal and decolouring method comprises the following steps: mixing a phosphate removing decolorant and waste water according to mass ratio, uniformly stirring under normal temperature, and standing for 20-90 minutes, wherein the phosphate removal rate of the waste water can reach 70-95.74, and the decolourization rate can reach 70-99.99. The method of the invention has the advantages of simple operation, convenient usage, good effect of phosphor removal and decolouring.

The invention relates to a method for decolorizing nucleotide enzymatic hydrolysate. The method comprises the following steps of: performing pigment adsorption on the nucleotide enzymatic hydrolysate through hyper-crosslinked resin SX-01; and after adsorption is saturated, and regenerating the hyper-crosslinked resin SX-01 by using a regenerant, wherein the hyper-crosslinked resin SX-01 has the following structure units: the skeleton of the resin is polystyrene, the average grain size is 0.3 to 1 mm, the water content is 32 to 49 percent, the aperture is 1.5 to 4 nm, the most probable aperture is 1.8 nm, the average specific surface area is 950 to 1,800 m<2> / g, and the average pore volume is 0.54 to 0.82 cm<3> / g. By adoption of the method, the decolorization ratio of the enzymatic hydrolysate reaches over 80 percent, the treatment quantity reaches 11 times of bed volume (BV), the loss ratio of four kinds of nucleotides is lower than 5 percent, and the consumption quantity of the regenerant is 5 BV.

The invention relates to a sugar juice decoloring agent and a sugar juice purification decoloring method. A preparation method of the sugar juice decoloring agent comprises the steps of preparing calcium phosphate in remelted syrup of brown granulated sugar, wherein nonsugar such as pigment in the remelted syrup of the brown granulated sugar is embedded in the calcium phosphate (equivalent to the template molecules); after that, removing the nonsugar such as pigment by firing at high temperature so as to obtain calcium phosphate with a macroporous structure, wherein big holes pass through a whole framework, the specific surface area is 169m<2> / g, and the average pore size is 500nm. The macroporous calcium phosphate is added into the remelted syrup of the brown granulated sugar as the sugar juice decoloring agent; the decolorization rate of the macroporous calcium phosphate for sugar juice reaches 38.15% under the conditions that the amount of the macroporous calcium phosphate is 0.2g, the reaction time is 30min, the reaction temperature is 80 DEG C, and the pH value is equal to 6.00. Compared with calcium phosphate generated in pure water, the decolorization rate of the macroporous calcium phosphate is increased by 10.1%.

The invention discloses a decoloring flocculant for treating soluble azo dye printing and dyeing wastewater and an application method thereof. A preparation method for the decoloring flocculant comprises the following steps of: (1) continuously stirring 50-80% by weight of inorganic high molecular flocculant in a breaker and heating for 5-10min; (2) adding 9-45% by weight of sulfate into the breaker, stirring for 10-30min at medium speed; (3) adding 0.01-0.05% by weight of quick sinking agent into the above substances, stirring, standing, depositing, and taking supernatant; (4) adding 5-10% by weight of surfactant into the supernatant to form an emulsion; and (5) diluting the emulsion by 10 times via distilled water to obtain clear transparent light yellow liquid. The transparent light yellow liquid in percentage by weight of 0.1-0.2% is added into the bright red soluble azo dye printing and dyeing wastewater, and the wastewater is quickly stirred for 1-3min at a speed of 300r / min, stirred for 10-15min at a speed of 80r / min, stood and deposited to obtain the colorless transparent printing and dyeing wastewater as the yielding water.

The invention discloses a heavy metal wastewater adsorbing material and a preparation method thereof. The heavy metal wastewater adsorbing material is characterized by being prepared from the following ingredients by weight parts: 4-6 parts of rectorite, 3-7 parts of kaolinite, 2-5 parts of polyacrylamide, 1-3 parts of aluminum sulfate, 1-2 parts of carboxymethyl chitosan, 1-2 parts of deacetylated chitin, 5-10 parts of ferric chloride, 5-10 parts of ammonia water, 1-2 parts of phosphatidylinositol, 2-4 parts of methyl parahydroxybenzoats, 0.2-0.6 part of sodium diacetate, 0.4-0.8 part of hydrogen peroxide, 0.1-0.6 part of peroxyacetic acid, 1-2 parts of sodium laurylsulfate, 80-100 parts of sodium hydroxide, 10-20 parts of ethylene glycol, 80-100 parts of ethyl alcohol, 0.1-0.2 part of sodium hydrosulfite, 70-100 parts of hydrochloric acid and 100-200 parts of water.

The invention discloses an irpex lacteus S-11 capable of efficiently degrading lignin, and belongs to the technical field of microbial engineering. A liquid medium containing 4 g / L lignin is inoculated with the strain, the strain is cultured at 28 DEG C at 180 rpm for 11 days, and the degradation rate and decolorization rate of the strain for lignin reach 33.81% and 41.79% respectively. The methodfor degrading the lignin by using the strain is convenient to operate, low in cost and suitable for biological pretreatment of lignin in biorefinery industry. The strain has the degradation rate of apure lignin substrate higher than that of same-species strains reported at home and abroad, is shorter in degradation time and is suitable for biological pretreatment application in the biorefinery industry.

The invention discloses a culture preparation method of trametes hirsuta laccase with a dyestuff synergistic degradation effect. The culture preparation method comprises the following steps: putting activated trametes hirsuta MX2 into a fermentation culture medium and culturing; centrifuging obtained fermentation liquid, wherein supernatant obtained by centrifuging is crude enzyme liquid; and separating and purifying the crude enzyme liquid to obtain two types of laccase isozymes which are marked as ThLac1 and ThLac2. The invention further provides a dyestuff de-coloring method which is carried out by utilizing the trametes hirsuta laccase; and the dyestuff de-coloring method comprises the following steps: mixing the ThLac1 with the ThLac2, wherein the ThLac1 accounts for 50%-80% of the total enzyme activity of a mixed enzyme; and putting the mixed enzyme into a dyestuff until the content is 50IU / L and de-coloring, wherein the de-coloring time is more than or equal to 3 hours.