The invention provides an automatic slice scanning device and a slice scanner, and relates to the field of biological medical devices. The automatic slice scanning device comprises a base, a luggage carrier, a lifting device, a first translation device and a slice carrier platform, wherein the luggage carrier, the lifting device, the first translation device and the slice carrier platform are installed on the base. The automatic slice scanning device and the slice scanner provided by the invention can achieve automatic focusing scanning, so that the operation is simple. Furthermore, in the device, a plurality of glass slides can be placed on the luggage carrier at the same time, after the pathological scanning of the previous slice is finished, the working procedure of the next slice is continued, batch scanning can be automatically performed, the scanning result is reliable, errors caused by human operation are reduced, the work efficiency is high, and unmanned operation and automaticscanning can be realized, so that the work intensity of workers is reduced, the comprehensive cost performance is high, and the automatic slice scanning device and the slice scanner can be promoted in large scale and are suitable for routine inspection projects.