The invention relates to a nano emulsion, which comprises, by weight percentage, 1-15% of a high HLB value emulsifier, 1-10% of a low HLB value emulsifier, 1-20% of solid grease, 5-25% of liquid grease, 1-15% of polyol and the balance deionized water. The invention also provides a preparation method of the nano emulsion. The method includes: firstly mixing the emulsifiers with grease to obtain an oil phase component, mixing the polyol with deionized water to obtain an aqueous phase component, respectively heating the oil phase component and the aqueous phase component in a water bath pot till complete dissolution, then adding the oil phase component into the aqueous phase component, adjusting the temperature until blue appears in the system obviously, recording the sample temperature at the moment, i.e. PIT (phase inversion temperature), conducting heat preservation for 0-10min, then stopping heating, and performing rapid cooling to room temperature, thus obtaining the nano emulsion prepared by phase inversion temperature method. The nano emulsion prepared by the method provided by the invention has uniform particles and good stability, and the energy consumption is greatly reduced in the preparation process, and the operation process and preparation cost are saved.