High efficiency nitrogen circulating bacteria artificial screening and application in aquatic state sepairing and water treatment

A nitrogen cycle and water ecology technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve problems such as the description of aquatic microorganisms, achieve enhanced tolerance, obvious effects, and avoid simple migration Effect

Inactive Publication Date: 2005-11-09
NANJING UNIV
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

For example, Zhang Zhi et al. invented an ecological restoration method for eutrophic small water bodies, which is mainly composed of eutrophic algae, black algae, sunflower, chara, and foxtail algae (Zhang Zhi, Zhou Zhongliang, Lin Chen, Lu Yajia et al., Ecological Restoration Method for Eutrophic Shallow Water, Patent Application No.: 03115845.5) applied for patent protection for the effect of various aquatic plants on purifying eutrophic water, but did not carry out patent application for the effect of aquatic microorganisms on purifying water quality. explain

Method used

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  • High efficiency nitrogen circulating bacteria artificial screening and application in aquatic state sepairing and water treatment
  • High efficiency nitrogen circulating bacteria artificial screening and application in aquatic state sepairing and water treatment
  • High efficiency nitrogen circulating bacteria artificial screening and application in aquatic state sepairing and water treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Collect bacterial water samples:

[0042] In various aquatic higher plant growth areas in lakes and other water bodies, use sterilized sterile test tubes (15×1.5cm) to extend into the water layer 20-30cm below the water surface to sample, seal immediately after sampling, and wait for inoculation in the laboratory.

[0043] 2. The first expansion training:

[0044] Prepare 200ml of culture medium for nitrifying bacteria, denitrifying bacteria, nitrosifying bacteria and ammonifying bacteria respectively, (wherein the medium composition of nitrifying bacteria is NaNO 3 1g, Na 2 CO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 0.5g, FeSO 4 0.4g, H 2 O 1000ml, pH value 8.2; The composition of nitrosative bacteria culture medium is (NH 4 ) 2 SO 4 0.2g, K 2 HPO 4 0.1g, MgSO 4 0.05g, NaCl 0.2g, FeSO 4 0.04g, CaCO 3 0.5g, H 2 O 100ml, pH value 7.2; The medium composition of ammonifying bacteria is: beef extract 4.5g, peptone 9.0g, NaCl 4.5g, H 2 O 1000ml,...

Embodiment 2

[0058] High-efficiency immobilized nitrogen cycle bacteria (ammonification-nitration-nitrosation-denitrification) are applied to eutrophication ecological engineering and ecological restoration of lakes, reservoirs and other water bodies (taking Hongfeng Lake in Guiyang as an example):

Embodiment 3

[0072] High-efficiency immobilization of nitrogen cycle bacteria (ammonification-nitration-nitrosation-denitrification) applied to flowing water bodies such as rivers (taking Guiyang Houwu Bay as an example)

[0073] Specific implementation plan:

[0074] A. Take water samples in the root zone of aquatic plants in natural water bodies to be inoculated

[0075] B. Carry out expansion cultivation, primary separation, enrichment, purification, and secondary enrichment according to Example 1 to obtain efficient nitrifying bacteria, nitrosifying bacteria, ammonifying bacteria, and denitrifying bacteria. The medium component of nitrifying bacteria is NaNO 3 1.1g, Na 2 CO 3 1.2g, NaCl 0.7g, K 2 HPO 4 0.6g, MgSO 4 0.6g, FeSO 4 0.5g, H 2 O 1000ml, pH value 8.5; The composition of nitrosative bacteria culture medium is (NH 4 ) 2 SO 4 0.3g, K 2 HPO 4 0.2g, MgSO 4 0.06g, NaCl 0.3g, FeSO 4 0.05g, CaCO 3 0.6g, H 2 O 100ml, pH value 7.5; the medium composition of a...

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Abstract

A process for effectively screening the nitrogen cycling bacteria used to repair the ecological environment of water and treat water includes such steps as acquiring the water sample containing local nitrogen cycling bacteria, respectively preparing the culture media for ammonifying bacteria, nitrosifying bacteria, nitrifying bacteria and denitrifying bacteria, culturing, separating, purifying and using the bacterium with high conversion rate as the preferable one.

Description

1. Technical field [0001] The invention belongs to the technical field of microbial artificial screening, and relates to artificial microbial screening and its application in environmental treatment, more specifically, it relates to the artificial screening of high-efficiency nitrogen cycle bacteria and its application in water ecological restoration and water treatment. 2. Background technology [0002] The pollution and eutrophication of lakes, rivers and other water bodies are serious ecological and environmental problems that endanger human health and restrict the sustainable development of social economy in many parts of China and even in the world. Investigations by the environmental monitoring department show that my country's seven major water systems, lakes, reservoirs, groundwater in some areas and coastal waters have been polluted to varying degrees, among which the problem of eutrophication is particularly prominent. Natural water bodies such as Dianchi Lake and T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 李正魁濮培民宁安洪宇宁
Owner NANJING UNIV
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