The invention relates to a
corn silk polysaccharide separation and decoloring method, which is characterized in that the method comprises the following steps: crushing corn silks, then mixing the corn silks with water in a
mass ratio of 1: 10 to 1: 15, adding
cellulase with the
corn silk mass of 1.0-1.5%, and regulating to a 45 DEG C of constant-temperature water bath with the pH value of 4.5-5.5; filtering
polysaccharide leaching liquor after enzymolysis, centrifugating, taking liquid supernatant for vacuum concentration, and removing
protein; adding powdered
activated carbon into a
polysaccharide solution after the removal of
protein and the 45 DEG C of constant-temperature water bath; after
centrifugation, taking the liquid supernatant, utilizing a microporous filter membrane for sucking
filtration for one time to three times, and removing the
activated carbon residue; adding
anhydrous ethanol or
methanol into the decolored silk polysaccharide solution, and centrifugating after uniform mixing;
drying precipitation in a vacuum mode to obtain
corn silk polysaccharide. The method can decolor
pigment in the solution in corn silk polysaccharide separation and extraction, so the effect is obvious, the
pigment removal rate can reach 85-90%, and the polysaccharide
retention rate can reach more than 83%. The process is simple. At the same time, the method effectively removes the
activated carbon left over in the decolored polysaccharide solution and maintains the purity of the polysaccharide color of the corn silk polysaccharide.