The invention provides an organic biochar enzyme inhibiting slow-release fertilizer and a production method thereof, and relates to the technical field of fertilizer production. The organic biochar enzyme inhibiting slow-release fertilizer is prepared from the following raw materials in parts by weight: 10 parts of potassium sulfate, 10 parts of monoammonium sulfate, 40 parts of urea, 18 parts of ammonium sulfate, 10 parts of a biochar enzyme inhibiting material, 5 parts of humic acid, and 7 parts of biochar powder, wherein the biochar enzyme inhibiting material is prepared from the following raw materials in parts by weight: 500 parts of polyaspartic acid, 400 parts of biochar powder, 5 parts of phosphate solubilizing bacteria, 5 parts of potassium bacteria, 60-80 parts of a nitrification inhibitor and 60-80 parts of a urease inhibitor. The organic biochar enzyme inhibiting slow-release fertilizer contains a certain amount of organic matters, and also comprises phosphate solubilizing bacteria, potassium bacteria, polyaspartic acid and urease inhibitor; with the biochar powder, propagation of microorganisms in soil is facilitated, the soil is improved, phosphate and potassium are solubilized, and loss of the slow-release fertilizer is controlled, so that not only is crop yield increased, but also waste recovery is realized, and the environmental pollution is reduced; the organic biochar enzyme inhibiting slow-release fertilizer belongs to environmental-friendly fertilizers.