Corn silk polysaccharide separation and decoloring method

A corn filigree and polysaccharide technology, applied in the field of plant polysaccharide separation and decolorization, can solve the problems of different effects, low pigment removal rate, difficult removal of activated carbon, etc., and achieves the effect of maintaining color and luster, simple process and remarkable effect.

Inactive Publication Date: 2010-06-23
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, each method has its limitations, and the decolorization effect of different plant polysaccharides is not the same
Activated carbon adsorption decolorization is difficult to remove; hydrogen peroxide oxidative decolorization, the effect is not ideal, and hydrogen peroxide has oxidative properties, which will destroy the biological activity of polysaccharides; Resin method, dialysis method and organic solvent method for the removal rate of pigments in polysaccharides not too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Grind the cleaned and dried corn filaments, weigh 50 g of the filament powder, add cellulase (enzyme activity 2000 units) of 1.0% of the filament mass, add distilled water at a ratio of 1:10, adjust the pH to 4.5, and place in a constant temperature water bath at 45°C. Stir for enzymatic hydrolysis for 5h. The filigree polysaccharide extract after enzymolysis is filtered with nylon mesh or gauze, centrifuged, the precipitate is discarded, the supernatant is taken, and vacuum concentrated to 1 / 3 volume by a rotary evaporator. The Sevage method is used to remove protein, and the specific operation is to mix the supernatant in the previous step: deproteinization reagent (chloroform: n-butanol = 5: 1) = 5: 1 (volume ratio), and centrifuge at 3500r / min for 10min. Take the supernatant. Add powdered activated carbon to the polysaccharide solution after deproteinization. The amount of activated carbon added is 0.08g / ml (polysaccharide solution after deproteinization). After mi...

Embodiment 2

[0018] The cleaned and dried corn filaments were pulverized, and 100 g of quantitative filament powder was weighed, cellulase (enzyme activity 1500) with a mass of 1.2% of the filaments was added, and distilled water was added in a ratio of 1:12.5. Adjust the pH value to 5.0 and place in a constant temperature water bath at 45°C for 4.5h. Filtrate the filigree polysaccharide extract with nylon mesh or gauze, centrifuge, discard the precipitate, take the supernatant, and use a rotary evaporator to vacuum concentrate to 1 / 3.5 volume. The Sevage method is used to remove protein, and the specific operation is to mix the supernatant in the previous step: deproteinization reagent (chloroform: n-butanol = 5: 1) = 5: 1 (volume ratio), and centrifuge at 4000r / min for 9min. Take the supernatant. Add powdered activated carbon to the polysaccharide solution after deproteinization. The amount of activated carbon added is 0.10g / ml (polysaccharide solution after deproteinization). After mix...

Embodiment 3

[0023] Crush the washed and dried corn filaments, weigh 200 g filament powder, add cellulase (enzyme activity 1000 units) with 1.5% filament mass, add distilled water at a ratio of 1:15, adjust the pH value to 5.5, and bathe in a constant temperature water bath at 45°C for 4 hours. Filtrate the filigree polysaccharide extract with nylon mesh or gauze, centrifuge, discard the precipitate, take the supernatant, and use a rotary evaporator to vacuum concentrate to 1 / 4 volume. The Sevage method is used to remove protein, and the specific operation is to mix the supernatant in the previous step: deproteinization reagent (chloroform: n-butanol = 5: 1) = 6: 1 (volume ratio), and centrifuge at 4500r / min for 8min. Take the supernatant. Add powdered activated carbon to the polysaccharide solution after deproteinization. The amount of activated carbon added is 0.12g / ml (polysaccharide solution after deproteinization). After mixing evenly, put it in a constant temperature water bath at 45...

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Abstract

The invention relates to a corn silk polysaccharide separation and decoloring method, which is characterized in that the method comprises the following steps: crushing corn silks, then mixing the corn silks with water in a mass ratio of 1: 10 to 1: 15, adding cellulase with the corn silk mass of 1.0-1.5%, and regulating to a 45 DEG C of constant-temperature water bath with the pH value of 4.5-5.5; filtering polysaccharide leaching liquor after enzymolysis, centrifugating, taking liquid supernatant for vacuum concentration, and removing protein; adding powdered activated carbon into a polysaccharide solution after the removal of protein and the 45 DEG C of constant-temperature water bath; after centrifugation, taking the liquid supernatant, utilizing a microporous filter membrane for sucking filtration for one time to three times, and removing the activated carbon residue; adding anhydrous ethanol or methanol into the decolored silk polysaccharide solution, and centrifugating after uniform mixing; drying precipitation in a vacuum mode to obtain corn silk polysaccharide. The method can decolor pigment in the solution in corn silk polysaccharide separation and extraction, so the effect is obvious, the pigment removal rate can reach 85-90%, and the polysaccharide retention rate can reach more than 83%. The process is simple. At the same time, the method effectively removes the activated carbon left over in the decolored polysaccharide solution and maintains the purity of the polysaccharide color of the corn silk polysaccharide.

Description

technical field [0001] The invention belongs to a plant polysaccharide separation and decolorization method, in particular to a corn filament polysaccharide separation and decolorization method. Background technique [0002] Corn silk (also known as corn silk) polysaccharide is sweet, flat, non-toxic, and has physiological functions such as lowering blood sugar, lowering blood pressure, anticancer, antibacterial and immune activity. Our country is rich in corn filament resources, and the filaments are easy to collect and the cost is low. Corn filaments are as effective as pollen. During the separation and extraction process, filigree polysaccharides are mixed with pigments, proteins and some small molecular substances. Among these impurities, pigments have a certain influence on the appearance quality of filigree polysaccharides, and make the separation, purification and structural identification of polysaccharides more difficult, so the pigments need to be removed. Durin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C08B37/00
Inventor 何余堂刘贺潘孝明宫照杰
Owner BOHAI UNIV
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