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Paenibacillus and application thereof to environmental engineering

The technology of Paenibacillus and Bacillus is applied in the application field of microbial strain resource application environmental engineering, Paenibacillus new species and the application field of environmental engineering, which can solve the problems of flocculation of strain harm, heavy metal ion toxicity and other problems, Achieve the effect of easy extraction, low production cost and high fermentation yield

Inactive Publication Date: 2010-10-27
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The dyes and heavy metal ions removed by bioflocculation ar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1: Cultivation and identification of Paenibacillus sp. Y24-1 CGMCC No. 3700

[0029] The Paenibacillus Y24-1 isolated and screened from Xinjiang desert sand samples was identified as a new species of Paenibacillus after the identification of microbial taxonomy. At present, the strain has been deposited in the International Depository of Microorganisms under the Budapest Treaty: The General Microbiology Center (CGMCC) of the China Microorganism Collection Management Committee (CGMCC) before the application date. Address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, zip code: 100101, the preservation date is March 26, 2010, and the preservation number is CGMCC No. 3700. After the identification of the multi-phase classification of microorganisms, it is determined that it is a new species of Paenibacillus, the biological name is tentatively designated Paenibacillus sp. Y24-1, and the recommended c...

Example Embodiment

[0034] Example 2: Tolerance of Paenibacillus sp. Y24-1 CGMCC No. 3700 to heavy metal ions

[0035] Inoculate Y24-1 strains with different concentrations of Pb 2+ , Cu 2+ , Co 2+ , Hg 2+ , Zn 2+ On the PDA solid medium plate, culture at a constant temperature of 30°C to detect its growth status. The measurement results are shown in Table 2.

[0036] Table 2 Tolerance experiment of Y24-1 strain to different heavy metals (concentration unit mg / L)

[0037]

[0038]

[0039] Note: + means growth,-means no growth

[0040] From the results in Table 2, it can be seen that the Y24-1 strain has a positive effect on Pb 2+ And Co 2+ The maximum tolerance range of >1200mg / L, Cu 2+ The maximum tolerance range is 100mg / L, Hg 2+ The maximum tolerance range is 50mg / L, Zn 2+ The maximum tolerance range of Y24-1 is 900mg / L, indicating that strain Y24-1 is resistant to Pb 2+ And Co 2+ Has a higher tolerance, and for Hg 2+ The tolerance is low.

Example Embodiment

[0041] Example 3: Adsorption activity of Paenibacillus sp. Y24-1 CGMCC No. 3700 on heavy metal ions during the cultivation process

[0042] In the tolerance range of the strain, different concentrations of Pb are formulated 2+ , Cu 2+ , Co 2+ , Hg 2+ , Zn 2+ PDA and Cha's fermentation medium, the concentrations of which are 1000mg / L, 100mg / L, 800mg / L, 50mg / L and 700mg / L, respectively, connected to Y24-1 strain and cultured at 30℃, 150r / min constant temperature shaking 48 Centrifuge at 6000 rpm for 5 minutes, take the supernatant to determine the content of different heavy metals, and calculate the bacteria's adsorption capacity for different heavy metals. The calculation formula for the adsorption rate is: r=(B 0 -B 1 ) / B 0 ×100%, where B 0 Is the concentration of heavy metals in the medium without adding bacteria, B 1 It is the concentration of heavy metals after adding bacteria liquid to culture. The results are shown in Table 3.

[0043] The determination method of different he...

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PUM

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Abstract

The invention discloses paenibacillus sp. Y24-1 CGMCC No. 3700 and application of flocculating agents generated by the paenibacillus to the aspect of removing suspended substances, coloring agents and heavy metal from waste water. The ion absorption rate of the paenibacillus is respectively 90.535 percent for Pb2<+>, 78.74 percent for Hg<2+>, 18.457 percent for Cu<2+>, 15.853 percent for Co<2+> and 19.365 percent for Zn<2+>. The method comprises the following steps: carrying out fermentation culture on strains; taking fermentation liquid for centrifugation; adding absolute ethyl alcohol with 1.5 times of the volume into supernatant fluid; carrying out still standing at 4 DEG C after the materials are uniformly mixed; carrying out centrifugation on separated precipitate; carrying out ethanol washing and vacuum drying on obtained precipitate to prepare the paenibacillus. The flocculation activity is between 81.7 and 83.15 percent. The invention has wide application in the aspect of removing suspended substances, coloring agents, heavy metal and the like from waste water.

Description

Technical field [0001] The invention belongs to the technical field of microbial strain resources application environmental engineering. Specifically, it relates to the technical field of a new species of Paenibacillus and its application in environmental engineering. Background technique [0002] At present, in environmental engineering technology, flocculation sedimentation method is one of the widely used and low-cost methods, and the key to this process is to select and add flocculants with excellent performance. There are three main types of flocculants. One is low-molecular-weight inorganic salts, such as aluminum salts and iron salts, which are used in large amounts, which are likely to cause secondary pollution and affect human health; the other is synthetic polymer flocculants, which have fast flocculation speed and high efficiency, but are difficult to biodegrade , Residual monomers are toxic and other issues; third, natural organic polymer flocculants have the advant...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C02F1/28C02F1/52C02F1/62C02F3/34C12R1/01
Inventor 谢玉清顾美英茆军唐琦勇
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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