59results about How to "High flocculation activity" patented technology

The invention discloses paenibacillus sp. Y24-1 CGMCC No. 3700 and application of flocculating agents generated by the paenibacillus to the aspect of removing suspended substances, coloring agents and heavy metal from waste water. The ion absorption rate of the paenibacillus is respectively 90.535 percent for Pb2<+>, 78.74 percent for Hg<2+>, 18.457 percent for Cu<2+>, 15.853 percent for Co<2+> and 19.365 percent for Zn<2+>. The method comprises the following steps: carrying out fermentation culture on strains; taking fermentation liquid for centrifugation; adding absolute ethyl alcohol with 1.5 times of the volume into supernatant fluid; carrying out still standing at 4 DEG C after the materials are uniformly mixed; carrying out centrifugation on separated precipitate; carrying out ethanol washing and vacuum drying on obtained precipitate to prepare the paenibacillus. The flocculation activity is between 81.7 and 83.15 percent. The invention has wide application in the aspect of removing suspended substances, coloring agents, heavy metal and the like from waste water.

The invention discloses a method to prepare bio-flocculant. The invention is characterized in that aspergillus fermentating cultivation is adopted. The aspergillus is agitated and fermented (28-30 DEG C, pH6-7, under ventilating condition) for 48-60 hours; the fermentation broth is filtered and condensed, and then proper amount of ethanol is added into the condensed broth and centrifugal separation is conducted after stewing; the deposition is washed with ethanol and dried in vacuum to achieve a crude product of fawn flocculant. The prevention method has the advantages of simple condition of production and low cost. The bio-flocculant prepared by the method is applicable to disposing sewage containing granular contaminants or filth, and the dosage generally drops in 0.1 to 0.3 per mill. It is proved by flocculation treatment that, compared with the prior art, the invention has sound flocculation effect, low flocculant speed and small dosage; besides, the invention is environment-friendly and harmless to human body.

The invention relates to an exopolysaccharide generated by pseudomonas, a culture method and application. The pseudomonas sp. QL212 is preserved in common microbe center of China Committee for Culture Collection of Microorganisms on September 12, 2014, at the address of Institute of Microbiology of Chinese Academy of Sciences, No.3, Yard No. 1, West Beichen Road, Chaoyang District, Beijing, and having the preservation number of CGMCC NO.9651. The curdlan synthesized by the pseudomonas sp. QL212 serves as the exopolysaccharide; through fermentation condition optimization, the yield of curdlan is up to 5.94 g/L which is higher than the production peak of the already known pseudomonas curdlan.

The invention relates to a Bacillus licheniformis gene engineering bacterium capable of producing a polysaccharide flocculant at high yield and an establishment method thereof, belonging to the technical fields of gene engineering and microbial fermentation. The gene engineering bacterium is formed by overexpressing epsDEF gene in Bacillus licheniformis. The nucleotide sequence of the epsDEF gene is disclosed as SEQ ID No 1 in the sequence table. The gene engineering bacterium preparation method comprises the following steps: cloning the epsDEF gene into an expression vector to obtain a recombinant expression vector, and transforming the recombinant expression vector into extracellular-polysaccharide-producing Bacillus licheniformis by electric transformation, thereby obtaining the gene engineering bacterium capable of producing a polysaccharide flocculant at high yield. The polysaccharide flocculant preparation method comprises the following steps: carrying out fermentation and culture on the gene engineering bacterium, collecting the fermentation liquid, and purifying to obtain the polysaccharide flocculant. In the gene engineering bacterium fermentation process, the flocculation activity of the fermentation liquid is obviously enhanced, and the polysaccharide flocculant yield is obviously enhanced. The polysaccharide flocculant is applicable to sewage treatment and food engineering.

The invention relates to bacillus licheniformis, a method for preparing a flocculating agent from the bacillus licheniformis and an application of the flocculating agent. The strain is bacillus licheniformis LZ-1 which is preserved in China General Microbiological Culture Collection Center on November 06, 2012 with preservation number of CGMCC No.6782. With the application of the bacillus licheniformis LZ-1 as well as a production method provided by the invention, a fermentation broth, which is high in flocculating activity, is obtained under the condition that an initial pH value is alkaline, and high-molecular-weight polysaccharide, which is 0.8-3.2*10<7>Da, serves as a major flocculating active ingredient. The polysaccharide flocculating agent provided by the invention has the advantages of being high in flocculating activity, low in dosage, low in production cost and the like, and the flocculating agent has a flocculating conditioning effect on sludge. The bacillus licheniformis LZ-1 fermentation broth or a product, which is extracted from the fermentation broth and contains the polysaccharide flocculating agent, can be applied to deep dewatering by sludge plate-frame pressure filtration in a mode of replacing a polyacrylamide flocculating agent, so that sewage treatment plant concentrated sludge, which is 96-98% in moisture content, is further dewatered into a sludge cake which is lower than 60% in moisture content and the size of the sludge is diminished to be 1/20-1/10 of an original size.

The invention discloses a multi-functional composite bioflocculant for wastewater treatment. The composite bioflocculant is prepared from the following raw materials in percentage by weight: 15 to 30 percent of aluminum polychlorid, 5 to 20 percent of ferrous sulfate, 5 to 20 percent of sodium hydrogen phosphate, 5 to 10 percent of magnesium sulfate, 2 to 5 percent of potassium chloride, 2 to 5 percent of zinc chloride, 1 to 2 percent of calcium carbonate and 50 percent of microbial flocculant. The composite bioflocculant has the characteristics of high-efficiency flocculation, easiness for microbial degradation and environment friendliness, also has certain microbial growth factors, and can be used for the growth and succession of microorganisms in the subsequent biochemical treatment process of wastewater as well as quick and mass propagation in severe environments, accelerate biochemical reaction and improve the treatment effect of biochemical systems.

The invention relates to a method for extracting a bioflocculant. The method is characterized by extracting the flocculant from activated sludge, has the advantages of wide source and low cost, comprising the following steps: screening the activated sludge and extracting bacterial strains with best activity, culturing in a glucose medium, and then carrying out extraction on the supernatant liquor in the medium by acetone process, ethanol process or CTAB process. The obtained flocculant has high activity, high yield, no secondary pollution to the environment, and very good potential of large scale production.

The invention discloses an industrial sewage treatment adsorbent. The industrial sewage treatment adsorbent comprises the following raw materials in parts by weight: 30-40 parts of attapulgite, 30-40 parts of argil, 20-30 parts of expanded graphite cyclodextrin compound, 10-18 parts of bamboo charcoal, 10-20 parts of carbon nanotube, 5-10 parts of retinervus luffae fructus, 8-18 parts of adhesive, 20-35 parts of flocculant and 3-5 parts of oxidizing bactericide. According to the invention, industrial sewage treatment is performed by means of the attapulgite clay; and the industrial sewage treatment adsorbent can adsorb wastewater containing heavy metal, is high in effect and low in cost, and achieves considerable economic benefits.

A method for producing biological flocculent by biological hydrogen waste liquid is carried out by: 1) mixing producing fungus substratum of hydrogen waste liquid and flocculent to prepare culturing solution, and circulating acclimating flocculent by rich and poor nutritive alternating method; 2) transferring acclimated bacterial strain to biological hydrogen waste liquid at temperature 30deg.C, culturing at table speed 140r/min, and finishing acclimating to obtain biological flocculent when flocculating ratio>50%. It achieves high efficiency and safety, and more effective.

The present invention in particular relates to a preparation method of biological type sludge conditioning agent and the application thereof. The biological type sludge conditioning agent is prepared by using aspergillus sojae (Aspergillus sojae) as raw material, and the obtained sludge conditioning agent can be applied to the sludge dewatering. The coagulation activity of the conditioning agent of the present invention is high, the cost is low, the dosage output is high, the stability is strong, the applicable range is wide, and the present invention has the biological degradability and the environmental friendliness, and the second pollution is not caused.

The invention provides a method for preparing a microbial flocculant by a protoplast fusion technology, and belongs to the field of flocculant preparation. The method is characterized by (1) protoplast producing bacterium LV-1 regeneration conditions and preparation steps, specifically comprising carrying out an activation operation in a liquid complete medium (CM) for 5 to 10 hours based on an inoculation amount of 0.5 to 2%, adding 2 to 6 mol/L of glycine into the liquid complete medium before enzymolysis to carry out a treatment for 5 to 15 hours, carrying out an enzymolysis process in a water bath for 1 to 4 hours at a temperature of 37 DEG C and an enzyme concentration of 0.05 to 0.2 mg/mL, and coating a regeneration template containing cane sugar as a stabilizer, and (2) protoplast fusion strain LV-2 fermentation and flocculation conditions, specifically comprising a cane sugar content of 8 to 12 g/L, a lactose content of 5 to 10 g/L, an ammonium nitrate content of 0.4 to 1.2 g/L, a peptone content of 0.4 to 1.2 g/t, an extract yeast content of 0.1 to 0.4 g/L, a culture temperature of 20 to 40 DEG C and an optimal adding amount of 0.2 to 2%. Compared with the prior art, the method has the advantages of high effectiveness, simple process, board application scope, low cost and good biological safety.

The invention discloses a compound oil paint flocculating agent where bacterial cellulose shell-core structures are added. The following steps including the first step of strain separation and purification, the second step of strain screening, the third step of strain fermentation and cultivation and the fourth step of compound magnetic microsphere preparation are included. The preparation process is simple, the compound oil paint flocculating agent can be suitable for industrialized large-scale production, can be repeatedly used and can reduce cost, the problems that the flocculating efficiency is poor and sediment sinks in circulating water are effectively solved, and the long-term effective safe operation of the circulating water is ensured.

The invention relates to an industrial ammonia-nitrogen organic wastewater treatment agent and a preparation method thereof, and belongs to the technical field of industrial wastewater treatment. By arranging a self-made zeolite carrier and multiple functional microorganisms, modifying the natural zeolite, compounding the microorganisms and cooperating with each other, the ammonia nitrogen and COD in the industrial ammonia nitrogen organic wastewater are effectively removed finally, the turbidity of the wastewater is remarkably reduced, and the treatment agent has a wide application prospect.

The invention discloses a method for preparing a bioflocculant and use thereof. The method comprises the following steps of: (a) performing fermentation culture on penicillium funiculosum to obtain fermentation liquor and centrifuging the fermentation liquor to remove mycelium to obtain supernate; and (b), mixing the supernate with ethanol and centrifuging the mixture to obtain the bioflocculant. The bioflocculant can be used for treating activated sludge, fly ash, charcoal, ink, muddy water, river sediment suspension, kaoline suspension, manure water, dyeing and printing wastewater and/or fruit juice.

The invention provides a preparation method and application for immobilized microorganism flocculant. The preparation method comprises the following steps: preparing aqueous solution containing 0.5-2% of sodium alginate, 0.5-1.5% of polyvinyl alcohol and 0.5-1.5% of gelatin according to the mass concentration; after the mixture is fully dissolved, adding Geotrichum candidum to ensure that the Geotrichum candidum accounts for 3-6wt% of the solution; evenly stirring; dripping into 0.05-0.15mol/L of CaCl2 solution prepared in advance; standing for 3-5h; and carrying out suction filtering and washing to obtain the immobilized microorganism flocculant. The immobilized microorganism flocculant has the advantages of high flocculation activity, small use amount, no secondary pollution and the like when being used for treating water, the mineralization flocculation rate for the underground karst water is about 70%, and the maximum hardness flocculation rate can be about 60%.

The invention relates to the technical field of polymer flocculant materials for sewage treatment, in particular to a starch-based polymer composite flocculant and a preparation method thereof. The preparation method of the composite flocculant comprises the following steps: drying potato peels in the sun and crushing the potato peels to obtain potato peel powder, adding distilled water to preparea turbid liquid, and adding a NaOH solution to activate chemical functional groups of the potato peel powder to obtain a solution A; adding hexamethylenediamine into the solution A, and conducting reacting at 40-60 DEG C for 1-2 hours to obtain a solution B; and adding a cross-linking agent dimethyl diallyl ammonium chloride and an initiator potassium persulfate into the solution B, conducting reacting at 30-60 DEG C for 2-3 hours, adding polyaluminium chloride accounting for 0.5-2% of the total mass of the system, and carrying out sufficient and uniform stirring to obtain a starch-based polymer composite flocculant product. The potato peel is used as a raw material and is cheap and easy to obtain; and flocculation activity is good, and a sedimentation rate is favorably improved.