The invention provides a method for preparing a microbial flocculant by a
protoplast fusion technology, and belongs to the field of flocculant preparation. The method is characterized by (1)
protoplast producing bacterium LV-1 regeneration conditions and preparation steps, specifically comprising carrying out an activation operation in a liquid complete medium (CM) for 5 to 10 hours based on an
inoculation amount of 0.5 to 2%, adding 2 to 6 mol / L of
glycine into the liquid complete medium before enzymolysis to carry out a treatment for 5 to 15 hours, carrying out an enzymolysis process in a water bath for 1 to 4 hours at a temperature of 37 DEG C and an
enzyme concentration of 0.05 to 0.2 mg / mL, and
coating a regeneration template containing
cane sugar as a stabilizer, and (2)
protoplast fusion strain LV-2
fermentation and
flocculation conditions, specifically comprising a
cane sugar content of 8 to 12 g / L, a
lactose content of 5 to 10 g / L, an
ammonium nitrate content of 0.4 to 1.2 g / L, a peptone content of 0.4 to 1.2 g / t, an extract
yeast content of 0.1 to 0.4 g / L, a culture temperature of 20 to 40 DEG C and an optimal adding amount of 0.2 to 2%. Compared with the prior art, the method has the advantages of
high effectiveness, simple process, board application scope, low cost and good
biological safety.