The invention provides a preparation method of a microbial flocculant for efficiently removing
antibiotics, which comprises the following steps: inoculating a Vilnelande
azotobacter AZ6 strain into
fermentation liquor, carrying out shake culture, and mixing the obtained
fermentation liquor with bacterial liquid with
glycerol to obtain preserved bacterial liquid; inoculating the preserved
bacteria solution into a new
fermentation solution, carrying out shake culture, centrifuging, and collecting a supernatant; adding absolute ethyl
alcohol into the supernate, uniformly mixing, standing, centrifuging and collecting precipitate; and freeze-
drying the precipitate to obtain the microbial flocculant. The invention also provides application of the microbial flocculant for removing
antibiotics in water. The microbial flocculant is used for removing
antibiotics in water. The microbial flocculant disclosed by the invention is applied to a mechanism of removing antibiotics through a synergistic effect of surface adsorption, charge
neutralization, adsorption bridging and sweeping and netting.