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Method for preparing microorganism flocculant

A microbial flocculant and flocculant technology, applied in the field of microbial science, can solve the problems of unstable flocculant properties, long fermentation cycle, and low flocculation efficiency, and achieve ideal flocculation effect, simple method, and high flocculation activity.

Inactive Publication Date: 2011-09-28
NORTHEASTERN UNIV LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although microbial flocculants have the characteristics of non-toxicity, no secondary pollution and biodegradability, due to their disadvantages such as high production cost, long fermentation cycle, low flocculation efficiency, and unstable properties of flocculants, they have not been developed yet. large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Use the river bottom mud as the strain source, prepare the medium according to the conventional method, and prepare the liquid medium according to the solid medium used for each single colony after the single colony is formed, and put the single colony into the liquid medium for cultivation according to the conventional method. prepared as microbial culture fluid.

[0050] Perform primary screening on the cultured bacteria. The primary screening method is: put 20ml of kaolin suspension with a concentration of 5g / L in the test tube, add 2 to 3 drops of the cultured microbial culture solution, shake, shake well, and let it stand for 5 minutes. , observe whether there is floc formation, and cultivate the bacteria with floc formation as microbial flocculant producing bacteria. The obtained bacterial strains with flocculation effect were isolated and purified by plate streaking method to obtain pure bacterial strains, which were prepared into microbial culture fluid, and the...

Embodiment 2

[0070] The obtaining method of Delftia acidovorus is the same as that in Example 1.

[0071] The preparation ratio of the liquid medium is 5 grams of soluble starch, NaNO 3 10 grams, K 2 HPO 4 0.5 g, MgSO 4 ·7H 2 0.1 g of O and 0.1 g of KCl were put into 1000 mL of distilled water and adjusted to pH=7.0 for preparation. Sterilize the liquid medium and 250mL Erlenmeyer flask by high-pressure steam; use the Erlenmeyer flask as a seed incubator, put 50mL of liquid medium after high-pressure steam sterilization into the seed incubator; inoculate the Acidovorax strain Put the seed incubator with liquid medium into the seed incubator, then put the seed incubator into the HZQ-C air bath shaker, cultivate it for 15h at 20°C and 180r / min, and obtain the Acidovorax delftii seeds culture medium.

[0072] The obtained Delftia acidovoria seed culture solution is inoculated into a microbial fermentation tank equipped with a liquid medium for fermentation, and the inoculation ratio is ...

Embodiment 3

[0083] The obtaining method of Delftia acidovorus is the same as that in Example 1.

[0084] The preparation ratio of the liquid medium is 20 grams of soluble starch, NaNO 3 8 grams, K 2 HPO 4 5 g, MgSO 4 ·7H 2 2 grams of O and 2 grams of KCl were put into 1000 mL of distilled water and adjusted to pH=8.0 for preparation. Sterilize the liquid medium and 250mL Erlenmeyer flask by high-pressure steam; use the Erlenmeyer flask as a seed incubator, put 50mL of liquid medium after high-pressure steam sterilization into the seed incubator; inoculate the Acidovorax strain Put the seed incubator with liquid medium into the seed incubator, then put the seed incubator into the HZQ-C air bath shaker, cultivate it for 12 hours at 35°C and 160r / min, and obtain the Acidovoria delftii seeds culture medium.

[0085] The obtained Delftia acidovoria seed culture solution is inoculated into a microbial fermenter equipped with a liquid medium for fermentation, the inoculation ratio is tha...

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PUM

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Abstract

The invention relates to a method for preparing a microbial flocculant which is characterized by: preparing the microbial flocculant through the steps of the preparation of liquid medium, the fermentation culture of the strain, the preparation of the flocculant crude product and flocculant refined product using the delftia acidovorans as the stain resource for preparing the microbial flocculant. The mehtod finds the delftia acidovorans capable of generating the microbial flocculant with a stable flocculability and a good flocculating effect for the first time in China. The screened flocculanthas advantages of high flocculating activity, few dosage, short fermentation time, simple extraction technology, which greatly reduces the production cost. The method for preparing the flocculant is simple and easy to apply. The experiment of wastewater treatment shows that the flocculating effect is ideal after using the flocculant.

Description

technical field [0001] The invention belongs to the field of microbial science, in particular to a method for preparing microbial flocculants. Background technique [0002] Flocculation technology is an important measure widely used in water supply, wastewater treatment, sludge dewatering and other treatment processes. The key to the flocculation effect depends on the choice of flocculant. For a long time, there are two main types of flocculants used in water supply and wastewater treatment: inorganic flocculants represented by aluminum salts, iron salts and their hydrolyzed polymers, and organic polymer flocculants represented by polyacrylamide and its derivatives. agent. Although inorganic and organic flocculants are widely used because of their good flocculation effect and low cost, people have to pay attention to the secondary pollution they cause to the environment during use. Studies have shown that aluminum ions in inorganic flocculants can easily cause Alzheimer's ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/52
Inventor 胡筱敏董怡华李亮姜彬慧付忠田叶舒帆朱茂森尚进
Owner NORTHEASTERN UNIV LIAONING
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