32 results about "Pantoea sp." patented technology

The invention relates to a sugarcane endogenous nitrogen-fixing bacteria strain and an application thereof. The classification of the strain is named as Pantoea sp.NN08200 with a preservation number of CGMCC NO.5438. The strain disclosed herein can significantly promote the growth of plants and revisiting various plant pathogenic fungi. According to the invention, after inoculating the strain to sugarcane to conduct tissue culture plantlet for 50 days, dry weight of the plants and the total nitrogen content are respectively increased by 46.74% and 41.5% over blank control group, the nitrogen fixation efficiency is 12.85%; the dry weight of the overground part of corn seedling of the inoculated strain NN08200 is increased by 74.5% over control group, the dry weight of the underground part is increased by 84.6%, the plant height is increased by 16.3%, the chlorophyll content is increased by 29.2%, and the nitrogen content is increased by 27.0%. The strain has the effects of association nitrogen fixation, production of hormone, and the like for promoting the growth of plants, and can be applied for producing nitrogen-fixing microbial agents and bio-organic fertilizers with the effects of promoting growth and inhibiting plant diseases.

The invention discloses a nitrilase N1 derived from pantoea sp.AS-PWVM4 and a gene thereof, a nitrilase N2 derived from arabidopsis thaliana and a gene thereof, a nitrilase N3 derived from acidovoraxfacilis 72W and a gene thereof, a nitrilase N4 derived from leptolyngbya sp. and a gene thereof, a nitrilase N5 derived from brassica oleracea var.oleracea and a gene thereof and a nitrilase N6 derived from camelina sativa and a gene thereof, and a method for preparing paracyanobenzoic acid as p-aminomethylbenzoic acid intermediate by using the nitrilase as a biological catalyst; resting cells ofthe corresponding nitrilases can be used for catalyzing 100g/L of substrate; the conversion rate is greater than 99%; the method has the obvious characteristics of mild reaction conditions, no pollution and simple process route, and has broad industrial application prospects.

The invention discloses a rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin and the preservation number of the Pantoea sp. Sd-1 is CGMCC No. 6698. The Pantoea sp. Sd-1 is a bacterial strain which can grow on a culture medium with alkali lignin as a unique carbon source. After the Pantoea sp. Sd-1 is cultured for four days in a liquid culture medium containing 3g/L alkali lignin and supplemented with 1% of glucose and 0.5% of peptone, the fading rate and the degradation of lignin respectively reach 52.38% and 69.06%. And in a liquid culture medium containing 10g/L rice straws and supplemented with 1% of glucose and 0.5% of peptone, the degradation of straws can reach 54.51 at most.

The invention discloses pantoea sp. W36-1, of which the preservation number is CGMCC No. is 3701, a flocculant prepared by the pantoea sp. W36-1 and application of the flocculant in removal of suspensions, dyeing agents and heavy metal in waste water. Extracellular products of the bacteria have an obvious absorption peak at 200 to 250nm, and polysaccharide solution can remove 54.89 percent of free groups in the bacteria. The method for preparing the pantoea sp. W36-1 comprises the following steps of: performing fermentation culture of the bacteria strain for 48 hours, centrifuging 100ml of fermentation solution at a rotation speed of 6,000r/min for 10 minutes, adding absolute ethanol, of which the volume is 1.5 times that of supernatant, into the supernatant, uniformly mixing the absolute ethanol and the supernatant standing the mixed solution at the temperature of 4 DEG C overnight, centrifuging the precipitate, washing the precipitate by 70-percent ethanol for 2 to 3 times, and then drying the precipitate to obtain the finished product. The flocculent activity of the fermentation culture product of the pantoea sp. W36-1 is 81.7 to 83.15 percent, the yield of the pantoea sp. W36-1 is 6g/L and can be widely used for removing suspensions, dyeing agents, heavy metal and the like in waste water.

The invention discloses a pantoea strain which is classified and named pantoea sp. and is preserved in China General Microbiological Culture Collection Center on July 6, 2016 with the preservation number of CGMCC No.12737. The strain can generate ammonia gas and IAA, solubilize phosphate, and increase the content of nitrogen, phosphorus and potassium in crops and rhizosphere soil thereof. The strain can be applied during growing of crops like wheat, maize and rice.

A dye molecule detection method includes the steps of (1) synthesizing gold nano particles through a method of in-situ reduction of chloroauric acid with a bacteria strain Pantoea sp.IMH; (2) with the gold nano particles as a surface enhanced Raman substrate, mixing the gold nano particles with dye molecules; and (3) detecting a Raman characteristic peak of the dye molecules through a portable Raman spectrometer, thereby achieving the detection of four kinds of dye molecules.

The invention relates to a cellulose degradation composite flora which is composed of bacterial species and fungal species. The composite flora horizontal bacterial community is mainly composed of fontibacillus, stenotrophomonas, paenibacillus and pantoea; and the fungal community is mainly composed of petromyces and nectria. The composite flora is obtained by adopting a subculture continuous culture method; wheat straw is added into a Mandels culture medium to serve as a unique carbon source and is obtained by screening litters under a perennial pinus sylvestris forest in a Maowusu sandy land; and the composite flora can be used for innocent treatment and resource utilization of agricultural wastes such as phellinusribis bran. The method overcomes the defects of low efficiency, poor constant value, weak competitive capacity with indigenous microorganisms, non-ideal practical application effect and the like of cellulose degrading bacteria in practical application in the prior art, is suitable for decomposing and composting phellinusribis bran which is difficult to degrade, and can quickly and efficiently realize harmless treatment and resource utilization of the agricultural wastesdifficult to decompose.

The invention relates to a microorganism aerobiotically reducing arsenic high-effectively, which is separated from a high-arsenic pollution region in Inner Mongolia, China. 16S rDNA identification proves that the microorganism belongs to pantoea agglomerans and is named Pantoea sp.IMH. A bacterial strain of the microorganism is assigned the accession number CGMCC No.8825. The microorganism is wide in pH and temperature ranges of growth, is very high in tolerance on trivalent arsenic and pentavalent arsenic, and can reduce more than 90% of the pentavalent arsenic in 36 h under an aerobiotical culture condition.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to an organic solid waste aerobic composting strain. The organic solid waste aerobic composting strain is identified as Pantoea sp., and has the preservation number of CCTCC NO: M 2020973. The pantoea Pantoeasp.BK-PP311 is resistant to high temperature, has the capacity of efficiently degrading organic matters and quickly decomposing, and can be applied to the high-temperature aerobic composting treatment process of organic solid wastes such as food wastes, kitchen wastes, fruit and vegetable wastes, livestock and poultry breeding feces, municipal sludge and the like, so that quick composting and resource utilization of the organic solid wastes are realized.

The invention discloses a bacterial synthetic community, which is composed of eight bacteria, namely Acinetobacter lwoffii, Bacillus amyloliquefaciens, Curptobacterium luteum, Curptobacterium sp., Methylobacterium platani, Pantoea sp., Pseudomonas parasulva and Pseudomonas sp., compared with a single bacterial strain, the bacterial synthetic community can better control the occurrence and progress of citrus green mold and sour rot, can more enduringly control diseases, can be used for preparing a biological control agent for controlling postharvest diseases of citrus fruits, has good application potential. A new thought and method are provided for controlling the postharvest diseases of the citrus fruits, and the application range of the bacterial synthetic community is widened.

The invention discloses an endophytic bacterium pantoea sp. CcSh-1 generating volatile fragrant substance, and the preservation number is CGMCCNo.8715. The bacterium strain comes from the sheath part of matured cymbopogon citratus, is easy to culture, and is capable of generating refreshing and pleasing fragrance after being grown on a solid medium for 2-3 days, and the fragrant substance is identified to contain a citral composition. The bacterium provides a new microbe source for replacing plant raw materials producing perfumes.

The invention relates to the technical field of environmental microorganisms, in particular to a compound microbial agent and application thereof in high-temperature aerobic composting of biogas residues. The compound microbial agent is prepared from the following components: Geobacillus variotii G6-1, Acinetobacter piteti pittii AP-16, Pantoea sp. BK-PP311 (Pantoea sp. BK-PP311), and Geobacillus kaustophilus FWGK-JYJ1 (Bacillus kaustophilus FWGK-JYJ1), and the compound microbial agent is prepared from the following raw materials: Geobacter variotii G6-1, Acinetobacter piteti pittii AP-16, Pantoea sp. BK-PP311, Geobacter kaustophilus FWGK-JYJ1, Pantoea sp. The compound microbial agent is used for high-temperature aerobic composting of biogas residues, can improve the highest temperature of a compost body, prolong the high-temperature period, quickly decompose organic matters, shorten the composting time and improve the decomposition degree of materials and the quality of organic fertilizers, and has relatively high application value.

The invention discloses quinclorac degradation bacterium and application thereof. The quinclorac degradation bacterium J7 provided by the invention belongs to pantoea sp. and is preserved in China Center for Type Culture Collection (CCTCC); the preservation date is March 16, 2017 and the preservation number is CCTCC M2017131; the quinclorac degradation bacterium is identified to be pantoea sp. by 16S rDNA. The bacterium is a Gram-negative bacterium and has a light yellow bacterial colony, a smooth and round surface and an ordered edge. The bacterium has a function of degrading quinclorac and can be used for preventing and controlling phytotoxicity, caused by the quinclorac, on tobaccos.

The invention provides a method for reducing heavy metal lead and cadmium in plants in river sand. The method comprises the following steps: (1) enriching strains; (2) screening strain; (3) domesticating strains; (4) enlarging culture; and (5) mixing phosphate rock powder and phosphate solubilizing bacteria according to a mass ratio of (1-4): 1 to obtain a complex microbial inoculant, wherein thecomplex microbial inoculant is added in an amount which is 0.5 percent of the mass of the river sand and is uniformly mixed. After the phosphate solubilizing bacteria and the ground phosphate rock arecombined for use, heavy metal enriched by plants can be reduced, and the growth of the plants is promoted. Phosphorus-solubilizing pantoea phosphorus-solubilizing bacteria separated, purified and domesticated from ground phosphate rock have a good function of decomposing inorganic phosphorus, and absorption of plants to heavy metals Pb and Cd in river sand is reduced by compounding the phosphorus-solubilizing pantoea phosphorus-solubilizing bacteria and the ground phosphate rock from which the phosphorus-solubilizing pantoea phosphorus-solubilizing bacteria are separated. The method uses fewexogenous chemical substances, is environment-friendly, and is a referential method for planting plants on the heavy metal polluted river sand.