21 results about "Alfalfa leaf" patented technology

The invention discloses a low-temperature-resistant leaf fertilizer for promoting reviving and growth of alfalfa as well as a preparation method and application of the leaf fertilizer, and belongs to the technical field of crop fertilizers. The invention discloses an alfalfa leaf fertilizer capable of resisting low temperature and promoting reviving and growth. The alfalfa leaf fertilizer comprises the following components in parts by weight: 40.0-50.0 parts of phosphorus and potassium-containing macroelement components; 1.0 to 3.0 parts of a Harpin immune protein; 1.0 to 3.0 parts of chitosan oligosaccharide; 0.5 to 1.0 part of gamma-aminobutyric acid; 10.0 to 20.0 parts of amino acid; 10.0 to 20.0 parts of glucose; 0.05 to 0.10 part of a plant regulating agent; 0.06 to 0.15 part of a surfactant; and 20 to 30 parts of water. By preferably selecting various low-temperature freezing injury resistant active components and phosphorus and potassium nutrients according to a specific ratio, the low-temperature freezing injury resistant capacity of the alfalfa is improved, the problem of repairing after low-temperature freezing injury of the alfalfa is solved, growth is quickly promoted, and a synergistic absorption effect is achieved.

According to the stress-resistant agent for alfalfa planting and the use method of the stress-resistant agent, the synergistic effect of betaine, glucose and monopotassium phosphate is utilized, and a seed soaking or foliage spraying mode is adopted, so that on one hand, expression of a series of stress-resistant related genes can be induced, ROS generated by stress excitation can be eliminated, and the stress resistance of alfalfa can be improved; the damage of reactive oxygen free radicals and the like to plasma membranes is reduced, the activity of antioxidant enzymes is enhanced, the accumulation of osmotic regulation substances is enhanced, and the chlorophyll synthesis of alfalfa leaves is remarkably improved, so that the stress resistance of plants is enhanced, and the damage of adversity stress to alfalfa plants is relieved. The stress-resistant agent is simple in component, free of pungent smell and good in stress-resistant effect, has the characteristics of no environmental pollution, zero emission and no residue, and can obviously promote germination of alfalfa seeds, regulate growth of alfalfa seedlings and improve stress resistance of the alfalfa seedlings.

Disclosed are equine supplement compositions for enhancing the health and well-being of horses. For instance, a composition may include a first portion containing one or more components selected from slippery elm, milk thistle, spirulina, marshmallow root, licorice root, alfalfa leaf, and ashwaganda; and a second portion containing one or more components selected from lysine, MSM, and goji berry. Additionally, a second composition may include an oil component selected from flax seed oil and coconut oil, combined with a honey component comprising manuka honey. Furthermore, a third composition may include a vitamin E component and a selenium component. The supplemental compositions provides a novel, cost-effective, simple, effective, efficient, and sustainable solution for supporting the overall health, performance, and longevity of horses by providing essential nutrients and beneficial compounds that target various aspects of equine health.

The present invention discloses the application of alfalfa MsTC protein and its encoding gene in regulating the vitamin E content of plants. The present invention constructs the MsTC gene in alfalfa onto a pBI121 vector to form a pBI121-MsTC recombinant vector, and then transforms the pBI121-MsTC recombinant vector into alfalfa "Zhongmu No. 1" by a method of alfalfa leaf disk Agrobacterium transformation to obtain transgenic alfalfa overexpressing MsTC. Through transgenic experiments, it was found that overexpressing the MsTC gene can significantly increase the vitamin E content of alfalfa, which is of great significance for improving the quality of alfalfa and promoting the stable and healthy development of animal husbandry.

The invention discloses an application of MsBBX25 protein and a coding gene thereof in regulating and controlling the content of vitamin E and crude protein in plants. According to the invention, the MsBBX25 gene in medicago sativa is constructed on a pBI121 vector to form a pBI121-MsBBX25 recombinant vector, and then the pBI121-MsBBX25 recombinant vector is transformed into medicago sativa 'Zhongshu No.1' by a method of transforming leaf discs of medicago sativa through agrobacterium, so that the MsBBX25 transgenic medicago sativa is obtained. Transgenic tests find that the overexpression of the MsBBX25 gene can significantly improve the alpha-tocopherol content, the total tocopherol content and the crude protein content of alfalfa, which is of great significance for improving the quality of alfalfa and promoting the stable and healthy development of animal husbandry.

The invention discloses a production regulator for planting alfalfa and a mixing device for preparing the production regulator, belongs to the technical field of plant growth regulators, and aims to solve the problems of more raw materials and higher cost of the conventional alfalfa growth regulators. The gibberellic acid, the diethyl aminoethyl hexanoate and the brassinolide in the production regulator can effectively promote growth and development of the alfalfa, so that the alfalfa can reach a higher growth level in a short time, the amino acid water-soluble fertilizer provides rich nutrition for the alfalfa, healthy growth and yield increase of the alfalfa are facilitated, and after the production regulator is used, the growth rate of the alfalfa is increased. The yield of the alfalfa can be predicted to be increased by 10%-15%, the production regulator is simple in formula, low in cost and simple in use method, the diluted regulator only needs to be uniformly sprayed on leaves of the alfalfa when the alfalfa grows to about 30 cm, and the economic value can be remarkably increased.

The invention relates to a biocontrol bacterium for preventing and treating alfalfa leaf spot disease, a biocontrol microbial agent and a preparation method and application thereof, and belongs to the field of microorganisms, the biocontrol bacterium MX282 belongs to Pantoea agglomerans, is preserved in China General Microbiological Culture Collection Center on June 23, 2025, has the preservation number of CGMCC No.34969, and has the preservation number of CGMCC No.34969. The biocontrol bacterium MX282 has the preservation number of CGMCC No.34969. The biocontrol bacterium MX282 has the preservation number of CGMCC No.34969 and the preservation number of CGMCC No.34969. Meanwhile, the invention discloses application of the biocontrol bacterium MX282 to prevention and treatment of alfalfa leaf spot disease and promotion of alfalfa growth.

The invention provides a plant polysaccharide with an immunomodulatory function, an extraction method thereof and an immunomodulator, and relates to the technical field of plant polysaccharides. The extraction method provided by the invention comprises the following steps: carrying out alcohol extraction on plant raw materials, and then separating alcohol extraction residues; carrying out water extraction on the alcohol extraction residues, and separating to obtain a water extract; carrying out concentration and alcohol precipitation on the water extract, and centrifuging to obtain a polysaccharide mixture; deproteinizing, dialyzing and freeze-drying the polysaccharide mixture to obtain plant polysaccharide; the plant raw material is one of clover leaves, fairy leaves and banana flowers. According to the extraction method provided by the invention, the plant polysaccharide with relatively high immunocompetence can be obtained, and the plant polysaccharide effectively activates immune effector cells such as macrophages and promotes secretion of cytokines through a bidirectional immunoregulation effect, so that safe raw materials can be provided for developing products for enhancing body defense and maintaining immune balance.

The invention belongs to the technical field of material preparation and water treatment, and provides a preparation method and application of a biochar supported bimetallic CuMnO2 heterogeneous Fenton-like catalyst, and the preparation method comprises the following steps: in an inert gas environment, heating alfalfa leaf powder, and cooling to obtain biochar; manganese salt, copper salt, water and strong base are mixed, and a bimetallic solution is obtained; mixing the bimetallic solution with the charcoal to obtain dispersion liquid; the dispersion liquid is subjected to a hydrothermal reaction, and biochar-loaded bimetal mixed liquid is obtained; and adjusting the biochar-loaded bimetallic mixed solution to be neutral by using dilute nitric acid, and then drying to obtain the CuMnO2 / biochar composite material. Compared with pure CuMnO2, the catalyst prepared by the method has the advantages that the agglomeration of CuMnO2 particles can be reduced and the specific surface area is increased by introducing the charcoal; meanwhile, the forbidden band width of CuMnO2 can be reduced by introducing the biochar, and the degradation efficiency of pollutants is greatly improved.

The invention discloses a preparation method of plant-derived carbon quantum dots with an anti-inflammatory imaging function, which comprises the following steps: selecting various anti-inflammatory plant raw materials such as isatis tinctoria leaves, pea leaves, broad bean leaves, alfalfa leaves, cornflower, honeysuckle, rheum officinale, selfheal, dandelion leaves, corn poppy, semen trichosanthis, wormwood and magnolia petals, and preparing the plant-derived carbon quantum dots with the anti-inflammatory imaging function through a hydrothermal method and a microwave method. The preparation method comprises the following steps: taking a plurality of plant raw materials with the anti-inflammatory effect as a carbon source, respectively adopting a raw material pretreatment method of homogenization and solvent extraction, and taking a hydrothermal method or a microwave method as a carbonization mode to prepare the plant-derived carbon quantum dots with the anti-inflammatory effect. The carbon quantum dot prepared by the method has the characteristics of small size, high fluorescence quantum yield, good anti-inflammatory and biocompatibility and the like, can be used as a carrier of anti-inflammatory drugs or anti-inflammatory nano-drugs, and has a good development prospect in the field of biological medicines.

The invention discloses a method for improving characters of alfalfa, which is characterized in that DELLA1 genes in the alfalfa are highly expressed by an agrobacterium-mediated method; the method for improving the characters of the alfalfa comprises the following steps: increasing branches of alfalfa plants; the fresh weight of alfalfa plants is increased; the leaf number of alfalfa plants is increased; the content of starch in alfalfa leaves is increased; the content of soluble protein in alfalfa leaves is increased; the content of soluble sugar in alfalfa leaves is increased. The method has application and popularization prospects.

The invention discloses a solid beverage for increasing lactation yield in a lactation period. A formula of the solid beverage comprises the following raw materials: anhydrous dextrose, maltodextrin, fennel powder, illicium verum powder, alfalfa leaf powder, ferric pyrophosphate, zinc citrate and vitamin B12. The fennel powder, the illicium verum powder and the clover leaf powder are used for stimulating prolactin secretion of puerperae, anhydrous dextrose, maltodextrin, ferric pyrophosphate, zinc citrate and vitamin B12 can enrich the taste of the beverage, and meanwhile, energy and trace elements required by daily life are provided for the puerperae, so that the product better conforms to the taste of the puerperae, the appetite of the puerperae is improved, and the health care effect of the puerperae is improved. The lactation condition of the puerpera is comprehensively improved.

The invention discloses an alfalfa leaf and stem separating and screening device and a using method thereof. The alfalfa leaf and stem separating and screening device comprises a supporting plate, a screening box, a third alfalfa stem collecting box, a second alfalfa leaf collecting box, a first alfalfa leaf collecting box, a third alfalfa leaf collecting box, a second alfalfa stem collecting box and a first alfalfa stem collecting box. The upper ends of the front faces of the first alfalfa leaf collecting box and the first alfalfa stem collecting box are fixedly connected with third supports. The device comprises a motor, a fourth bevel gear, a second filter screen cover, a third support, a first gear disc, a first support, a first bevel gear, a third alfalfa stem collecting box, a second alfalfa leaf collecting box, a first alfalfa leaf collecting box, a third alfalfa leaf collecting box, a second alfalfa stem collecting box, a first alfalfa stem collecting box, a screening box, a second gear disc and a first filter screen cover. According to the multi-stage screening device, the purpose of multi-stage screening can be achieved through a first support, a sixth support, a first fan blade, a baffle, a second fan blade, a driven conveying roller, a conveying belt and a driving conveying roller.

The application discloses a kind of MsGDSL1 The application discloses application of a gene in regulating plant leaf size and yield, and belongs to the technical field of plant genetic engineering. MsGDSL1 The application clones a gene involved in regulating alfalfa leaf size by using transcriptome analysis. MsGDSL1 Experimental data show that, compared with wild type control plants, the leaf area of alfalfa MsGDSL1 gene overexpression plants is enlarged by an average of 60.54%, the fresh grass yield of transgenic plants is increased by an average of 58.97%, and the dry grass yield is increased by an average of 61.88%; and the leaf area of Arabidopsis overexpression lines is enlarged by an average of 4 times compared with wild type. MsGDSL1 MsGDSL1 MsGDSL1 MsGDSL1 MsGDSL1 MsGDSL1 MsG This result further indicates that the function of the gene in regulating plant leaf size is highly conserved across species, and proves the core value of the gene in improving the yield of alfalfa.

The present application relates to the field of biotechnology, and more particularly to a kind of alfalfa transient expression cellulase and its application in Xinguan fruit leaf active substance extraction, comprising the following steps: (1) cellulase coding gene construction vector, transformation agrobacterium, culture, centrifugation, collection of bacteria, suspended in buffer, dilution;(2) the bacteria solution obtained in step (1) is used to carry out vacuum infiltration on alfalfa leaf, culture, and 1-500 μM melatonin is sprayed on alfalfa leaf, and spraying is carried out once a day;(3) the alfalfa leaf obtained in step (2) is ground, homogenized, centrifuged, and supernatant is extracted to obtain cellulase enzyme solution;Melatonin is applied in the process of expression, and the activity of cellulase obtained is significantly improved, the problems of low activity of plant expressed cellulase and extraction of leaf active substance biological enzyme are solved;And the steps are simple, the cellulase prepared does not contain endotoxin, can be used for Xinguan fruit leaf active substance extraction, and has wide application prospect.

The invention relates to the technical field of functional food, in particular to a tendon-nourishing and bone-strengthening composition and a preparation method thereof, and the tendon-nourishing and bone-strengthening composition comprises a fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, clover leaf saponin, a selenium yeast-pachymaran compound, glucosamine, compound nuts, vitamin D, a beef-pork compound, a traditional Chinese medicine compound and cheese; the three functional components of the fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, the clover leaf saponin and the selenium yeast-pachymaran compound cooperate to effectively block the accumulation and toxic effect of harmful heavy metals in the Chinese herbal medicines in the human body, and normal absorption of trace elements necessary for the human body is not interfered at the same time.

The invention discloses a method for individual marking and fertility determination of alfalfa leafcutter bees. The method comprises the following steps: selecting a marking object, fixing the insect body, marking the chest, and mixing male and female adults to mark the individual alfalfa leafcutter bees. The method comprises the following steps: placing beehives and nest boards, transporting male and female adults, releasing them in the field, monitoring nesting activities, and calculating fertility indicators to evaluate the fertility of the alfalfa leafcutter bees. According to tests, the method for individual marking and fertility determination of alfalfa leafcutter bees provided by the invention has little effect on the flight of adult alfalfa leafcutter bees, and 98.13% of female insects can fly freely. In addition, the method for individual marking and fertility determination provided by the invention has a large sample size that can be marked, a high degree of visualization, and can determine the number of nests built throughout the life history of the alfalfa leafcutter bees, which is of great significance for the artificial breeding of alfalfa leafcutter bees.

The present invention discloses a method for recovering honeycomb cells and monitoring offspring development of alfalfa leafcutter bees, comprising the following steps: 1. placing honeycombs and hanging honeycomb panels; 2. inserting paper straws into the honeycomb panels; 3. releasing adult bees; 4. recovering paper straws; 5. cultivating larvae; 6. cultivating prepupae; and 7. performing data statistics and analysis. The method for recovering honeycomb cells and monitoring offspring development provided by the present invention can promptly and quickly recover newly constructed honeycombs by female bees, monitor the development of offspring in real time, and obtain information such as the diapause rate, offspring mortality rate, natural enemy damage rate, empty nest rate, and pollen ball percentage of offspring recovered within a selected time period. This provides a simple and rapid monitoring technology for timely and accurate understanding of the causes of bee losses during the propagation and application of alfalfa leafcutter bees. The method provided by the present invention can guide those skilled in the art to take timely measures to reduce offspring losses, and provide more efficient and high-quality pollination services for alfalfa seed production by protecting and utilizing alfalfa leafcutter bees.

This application discloses a method for fusing tetraploid alfalfa with diploid alfalfa and alfalfa mesophyll protoplasts, comprising the following steps: 1) enzymatically digesting cotyledon strips of tissue-cultured seedlings of tetraploid alfalfa and diploid alfalfa respectively to obtain a mesophyll protoplast mixture, and preparing pure tetraploid protoplasts and pure diploid protoplasts from the mesophyll protoplast mixture; 2) resuspending the pure tetraploid protoplasts and pure diploid protoplasts respectively using W5 buffer to obtain tetraploid suspension and diploid suspension, mixing the tetraploid suspension and diploid suspension at a volume ratio of 1:1, and then electrofusion to induce protoplast fusion, and allowing the protoplasts to stand after electrofusion to complete the fusion. This method can achieve a fusion rate of 5.98% between tetraploid alfalfa mesophyll protoplasts and diploid alfalfa mesophyll protoplasts, and a fusion rate of 5.49% between tetraploid alfalfa mesophyll protoplasts and diploid alfalfa mesophyll protoplasts.

This invention discloses a MsMYB-ZD The application of genes in regulating alfalfa leaf size and yield falls under the field of plant genetic engineering. This invention utilizes transcriptome analysis to clone a gene that regulates alfalfa leaf size. MsMYB-ZD Transcription factors; experimental data show that, MsMYB-ZD The gene exhibits a gradually upregulated expression pattern during leaf development; compared to wild-type alfalfa Gongnong 1 (1-16) individual plants, overexpression... MsMYB-ZD MsMYB-ZD The leaf area of ​​the transgenic plants increased by an average of 77%, and the expression level of the transgenic lines showed a significant positive correlation with the leaf area. The fresh grass yield of the transgenic plants increased by 158.7%, and the dry grass yield increased by 207.5%. These results indicate that this gene is of great significance for improving alfalfa yield.

This invention provides a strain MC-M1 for controlling Artemisia weeds, a biological herbicide, and its application, belonging to the field of Artemisia control technology. The gene sequence of strain MC-M1 is shown in SEQ ID NO: 1, and it was isolated from the leaves of naturally infected alfalfa. The fermentation broth of this strain showed a 100% inhibition rate on the germination of Artemisia seeds and an 83.33% disease incidence rate after spraying potted Artemisia. When its fermentation broth was mixed with a 1:1 volume ratio of bensulfuron-methyl solution at a bensulfuron-methyl concentration of 0.1165 g / L, the resulting compound microbial herbicide improved the control effect of Artemisia to 100%, significantly better than the single treatment, achieving synergistic effect. This provides a new strain resource and technical solution for the green control of Artemisia weeds, with good application prospects and ecological value.