10 results about "Alfalfa leaf" patented technology

The invention discloses a production regulator for planting alfalfa and a mixing device for preparing the production regulator, belongs to the technical field of plant growth regulators, and aims to solve the problems of more raw materials and higher cost of the conventional alfalfa growth regulators. The gibberellic acid, the diethyl aminoethyl hexanoate and the brassinolide in the production regulator can effectively promote growth and development of the alfalfa, so that the alfalfa can reach a higher growth level in a short time, the amino acid water-soluble fertilizer provides rich nutrition for the alfalfa, healthy growth and yield increase of the alfalfa are facilitated, and after the production regulator is used, the growth rate of the alfalfa is increased. The yield of the alfalfa can be predicted to be increased by 10%-15%, the production regulator is simple in formula, low in cost and simple in use method, the diluted regulator only needs to be uniformly sprayed on leaves of the alfalfa when the alfalfa grows to about 30 cm, and the economic value can be remarkably increased.

The invention relates to a biocontrol bacterium for preventing and treating alfalfa leaf spot disease, a biocontrol microbial agent and a preparation method and application thereof, and belongs to the field of microorganisms, the biocontrol bacterium MX282 belongs to Pantoea agglomerans, is preserved in China General Microbiological Culture Collection Center on June 23, 2025, has the preservation number of CGMCC No.34969, and has the preservation number of CGMCC No.34969. The biocontrol bacterium MX282 has the preservation number of CGMCC No.34969. The biocontrol bacterium MX282 has the preservation number of CGMCC No.34969 and the preservation number of CGMCC No.34969. Meanwhile, the invention discloses application of the biocontrol bacterium MX282 to prevention and treatment of alfalfa leaf spot disease and promotion of alfalfa growth.

The invention provides a plant polysaccharide with an immunomodulatory function, an extraction method thereof and an immunomodulator, and relates to the technical field of plant polysaccharides. The extraction method provided by the invention comprises the following steps: carrying out alcohol extraction on plant raw materials, and then separating alcohol extraction residues; carrying out water extraction on the alcohol extraction residues, and separating to obtain a water extract; carrying out concentration and alcohol precipitation on the water extract, and centrifuging to obtain a polysaccharide mixture; deproteinizing, dialyzing and freeze-drying the polysaccharide mixture to obtain plant polysaccharide; the plant raw material is one of clover leaves, fairy leaves and banana flowers. According to the extraction method provided by the invention, the plant polysaccharide with relatively high immunocompetence can be obtained, and the plant polysaccharide effectively activates immune effector cells such as macrophages and promotes secretion of cytokines through a bidirectional immunoregulation effect, so that safe raw materials can be provided for developing products for enhancing body defense and maintaining immune balance.

The invention discloses a method for improving characters of alfalfa, which is characterized in that DELLA1 genes in the alfalfa are highly expressed by an agrobacterium-mediated method; the method for improving the characters of the alfalfa comprises the following steps: increasing branches of alfalfa plants; the fresh weight of alfalfa plants is increased; the leaf number of alfalfa plants is increased; the content of starch in alfalfa leaves is increased; the content of soluble protein in alfalfa leaves is increased; the content of soluble sugar in alfalfa leaves is increased. The method has application and popularization prospects.

The application discloses a kind of MsGDSL1 The application discloses application of a gene in regulating plant leaf size and yield, and belongs to the technical field of plant genetic engineering. MsGDSL1 The application clones a gene involved in regulating alfalfa leaf size by using transcriptome analysis. MsGDSL1 Experimental data show that, compared with wild type control plants, the leaf area of alfalfa MsGDSL1 gene overexpression plants is enlarged by an average of 60.54%, the fresh grass yield of transgenic plants is increased by an average of 58.97%, and the dry grass yield is increased by an average of 61.88%; and the leaf area of Arabidopsis overexpression lines is enlarged by an average of 4 times compared with wild type. MsGDSL1 MsGDSL1 MsGDSL1 MsGDSL1 MsGDSL1 MsGDSL1 MsG This result further indicates that the function of the gene in regulating plant leaf size is highly conserved across species, and proves the core value of the gene in improving the yield of alfalfa.

The present application relates to the field of biotechnology, and more particularly to a kind of alfalfa transient expression cellulase and its application in Xinguan fruit leaf active substance extraction, comprising the following steps: (1) cellulase coding gene construction vector, transformation agrobacterium, culture, centrifugation, collection of bacteria, suspended in buffer, dilution;(2) the bacteria solution obtained in step (1) is used to carry out vacuum infiltration on alfalfa leaf, culture, and 1-500 μM melatonin is sprayed on alfalfa leaf, and spraying is carried out once a day;(3) the alfalfa leaf obtained in step (2) is ground, homogenized, centrifuged, and supernatant is extracted to obtain cellulase enzyme solution;Melatonin is applied in the process of expression, and the activity of cellulase obtained is significantly improved, the problems of low activity of plant expressed cellulase and extraction of leaf active substance biological enzyme are solved;And the steps are simple, the cellulase prepared does not contain endotoxin, can be used for Xinguan fruit leaf active substance extraction, and has wide application prospect.

The invention relates to the technical field of functional food, in particular to a tendon-nourishing and bone-strengthening composition and a preparation method thereof, and the tendon-nourishing and bone-strengthening composition comprises a fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, clover leaf saponin, a selenium yeast-pachymaran compound, glucosamine, compound nuts, vitamin D, a beef-pork compound, a traditional Chinese medicine compound and cheese; the three functional components of the fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, the clover leaf saponin and the selenium yeast-pachymaran compound cooperate to effectively block the accumulation and toxic effect of harmful heavy metals in the Chinese herbal medicines in the human body, and normal absorption of trace elements necessary for the human body is not interfered at the same time.

This application discloses a method for fusing tetraploid alfalfa with diploid alfalfa and alfalfa mesophyll protoplasts, comprising the following steps: 1) enzymatically digesting cotyledon strips of tissue-cultured seedlings of tetraploid alfalfa and diploid alfalfa respectively to obtain a mesophyll protoplast mixture, and preparing pure tetraploid protoplasts and pure diploid protoplasts from the mesophyll protoplast mixture; 2) resuspending the pure tetraploid protoplasts and pure diploid protoplasts respectively using W5 buffer to obtain tetraploid suspension and diploid suspension, mixing the tetraploid suspension and diploid suspension at a volume ratio of 1:1, and then electrofusion to induce protoplast fusion, and allowing the protoplasts to stand after electrofusion to complete the fusion. This method can achieve a fusion rate of 5.98% between tetraploid alfalfa mesophyll protoplasts and diploid alfalfa mesophyll protoplasts, and a fusion rate of 5.49% between tetraploid alfalfa mesophyll protoplasts and diploid alfalfa mesophyll protoplasts.

This invention discloses a MsMYB-ZD The application of genes in regulating alfalfa leaf size and yield falls under the field of plant genetic engineering. This invention utilizes transcriptome analysis to clone a gene that regulates alfalfa leaf size. MsMYB-ZD Transcription factors; experimental data show that, MsMYB-ZD The gene exhibits a gradually upregulated expression pattern during leaf development; compared to wild-type alfalfa Gongnong 1 (1-16) individual plants, overexpression... MsMYB-ZD MsMYB-ZD The leaf area of ​​the transgenic plants increased by an average of 77%, and the expression level of the transgenic lines showed a significant positive correlation with the leaf area. The fresh grass yield of the transgenic plants increased by 158.7%, and the dry grass yield increased by 207.5%. These results indicate that this gene is of great significance for improving alfalfa yield.

This invention provides a strain MC-M1 for controlling Artemisia weeds, a biological herbicide, and its application, belonging to the field of Artemisia control technology. The gene sequence of strain MC-M1 is shown in SEQ ID NO: 1, and it was isolated from the leaves of naturally infected alfalfa. The fermentation broth of this strain showed a 100% inhibition rate on the germination of Artemisia seeds and an 83.33% disease incidence rate after spraying potted Artemisia. When its fermentation broth was mixed with a 1:1 volume ratio of bensulfuron-methyl solution at a bensulfuron-methyl concentration of 0.1165 g / L, the resulting compound microbial herbicide improved the control effect of Artemisia to 100%, significantly better than the single treatment, achieving synergistic effect. This provides a new strain resource and technical solution for the green control of Artemisia weeds, with good application prospects and ecological value.