35 results about "Alfalfa leaf" patented technology

The invention discloses a method for extracting alfalfa leaf protein by an ultrasonic-assisted enzyme process, belonging to the technical field of vegetable proteins. The method comprises the following steps: cleaning alfalfa leaves in early flowering stage as the raw material, pulping, and filtering to obtain a juice and pulp dregs; treating the juice by an ultrasonic-assisted enzyme process; adding cellulase into the juice while carrying out ultrasonic extraction to obtain the extracting solution, wherein 3-4g of cellulase is added to every 100ml of juice, the ultrasonic power is 100-250W, the ultrasonic frequency is 20-40kHz, the temperature is 50-55 DEG C, and the holding time of the ultrasonic action is 30-40 minutes; inactivating the enzyme, and flocculating the solution; carrying out continual treatment under the 4000-5000 r / min centrifugal action for 15-20 minutes to obtain a leaf protein crude extract and a supernatant; and extracting the leaf protein crude extract, and drying to constant weight, thereby obtaining the alfalfa leaf protein product. The invention has the advantages of simple technical process, mild conditions, low energy consumption, short extraction time and high extraction rate, is easy to operate and control, and is convenient for large-scale industrial production.

The invention relates to the technical field of feed additives, and aims to provide a composite chicken feed additive taking Chinese herbal medicines as main raw materials. The composite chicken feed additive is composed of the following components in parts by weight: 20-45 parts of folium cortex eucommiae, 12-30 parts of motherwort herbs, 10-25 parts of cordate houttuynia, 10-20 parts of mint leaves, 5-15 parts of clover-leaves, 5-15 parts of dandelion, 5-15 parts of beautyberry leaves, 3-10 parts of fructus forsythiae and 3-10 parts of radix bupleuri. The preparation method comprises the following steps: by taking folium cortex eucommiae, motherwort herbs, cordate houttuynia, mint leaves, clover-leaves, dandelion, beautyberry leaves, fructus forsythiae and radix bupleuri which are fresh and are not eroded by plant diseases and insect pests as raw materials, respectively carrying out vacuum drying on each raw material, and crushing each raw material into powder with a size of less than 80 meshes; and preparing the raw materials into the chicken feed additive in the mass ratios. The feed additive can effectively enhance the immunocompetence of chickens, promote the growth of broiler chickens, increase the laying rate of laying hens and improve the quality of eggs and chicken.

The invention relates to the technical field of a feed additive and particularly relates to a compound type pig feed additive as well as preparation and application methods thereof. The compound type pig feed additive is formed by the following components in parts by weight: 50-85 parts of eucommia ulmoides leaf powder, 10-30 parts of alfalfa leaf powder and 5-20 parts of folium callicarpae pedunculalae leaf powder. The compound type pig feed additive is prepared by taking fresh eucommia ulmoides leaves, alfalfa leaves and folium callicarpae pedunculalae leaves, which are not corroded by plant diseases and insect pests as raw materials, crushing the leaves into powder of being less than 0.5 mm after drying the leaves in vacuum, and mixing according to the mass ratios. The feed additive has the advantages that the immunity of live pigs is effectively improved, the growth of the live pigs is accelerated and the meat quality is improved.

The invention discloses an alfalfa leaf extract preparation method and a use as a tobacco product additive. The method comprises the steps of using alfalfa leaves as extraction raw materials, and uniformly mixing alfalfa leaves with extracting agent according to volume ratio of 1:(10-30) to obtain mixture; extracting the mixture for 1-5h at 50-90DEG C and performing separation to obtain primary material liquid; adding water into the material liquid, separating sediment, performing separation to obtain secondary material liquid and concentrating the secondary material liquid to extract; redissolving the extract by using 5-10 multiples of extracting solution, filtering and separating byproducts and tertiary material liquid, and concentrating the tertiary material liquid to extract to obtain alfalfa leaf extract. By adding the alfalfa leaf extract prepared by adopting the method disclosed by the invention into tobacco products, an effect of effectively increasing the flavor of the tobacco products is achieved, the flavor is rich and the solidity of smoke is increased; the extract can also endow the tobacco products with unique flavor tones and notes, and can be used as a characteristic flavor.

The invention discloses a method for extracting cloverleaf protein, which comprises that the cloverleaf of clover at squaring stage is taken as raw material and is pulped after being washed; the pulped cloverleaf slurry is filtered to obtain slurry liquid and filter residue; the obtained slurry liquid is treated by fermentation, lactic acid bacillus is adopted as zymolytic strain, the dosage of inoculation is 10<7>-10<9> of lactic acid bacillus viable cell used in every milliliter of the slurry liquid, the fermentation temperature is 30-39 DEG C, and the fermentation time lasts from 8 to 10 hours; the product which is treated by fermentation is continuously processed for 8-12min under the 300r / min of centrifugal effect, and crude extract of leaf protein and the supernatant fluid are obtained by flocculation after treatment; the crude extract of leaf protein is extracted and is treated by freeze drying, and cloverleaf protein products are obtained after the treatment. The method has simple technique and high content of extracted leaf protein; as heating is not needed, energy consumption is low; furthermore, acid, alkali or organic solvent, and the like are not needed to add, the obtained leaf protein is avoided being polluted.

The invention provides an alfalfa leaf protein moistening facial mask. The facial mask is a transparent collagen moistening facial mask and is prepared from the following components in parts by mass: 0.05-5 parts of alfalfa leaf protein serving as a functional raw material, 0.2-10 parts of sodium carboxymethylcellulose, 0.2-10 parts of humectant, 0.05-10 parts of starch and 1-20 parts of a glutaraldehyde crosslinking agent, wherein the sodium carboxymethylcellulose, humectant, starch and glutaraldehyde crosslinking agent are used as auxiliary raw materials. The preparation method comprises the following steps: firstly, extracting alfalfa leaf protein from alfalfa leaves in a fermenting manner; then mixing the alfalfa leaf protein and the auxiliary raw materials; and finally, putting the mixture in a die, heating and forming a film. The alfalfa leaf protein moistening facial mask is mainly prepared from macromolecular substances in natural animal and plant, is non-toxic and odorless, and does not have side effect. The facial mask has the effects of double moistening effect, good toughness, good ductility and good adhesion, and has functions of better skin feeling and deep skin cleaning, thus being an economical, practical and effective skin-care beautifying cosmetic. The facial mask preparation process is simple, is convenient to operate, and does not pollute the environment.

The invention relates to nutrient beer having the green tea flavor and a brewing method thereof and belongs to the field of food processing. The 15-20 parts of green tea powder and 5-10 parts of alfalfa leaf protein are added, high-activity dried yeast is added for fermentation after the green tea powder, the alfalfa leaf protein and malt juice are mixed, the beer obtained after fermentation has the strong green tea fragrance flavor, bitter generated through hop fermentation is counteracted by the green tea flavor, and the alfalfa leaf protein contains the amino acid necessary for the human body; meanwhile, the alcoholic strength of the beer is lower than that of beer not containing green tea powder and alfalfa leaf protein. The nutrient beer having the green tea flavor and the brewing method thereof solve the problems that existing beer has only one taste and high in alcohol strength; due to the fact that the alfalfa leaf protein is added in the beer, the application range of the alfalfa leaf protein is widened, and the beer is made into a nutrient health-care product rich in protein.

The invention discloses medicago slices, which comprise the following components by weight: 80-100 parts of medicago leaf freeze-drying powder, 80-100 parts of hamburger coffee Xiang Maicai freeze-drying powder, 10-12 parts of matrimony vine powder, 5-10 parts of chrysanthemum powder, 8-12 parts of calcium citrate, 3-5 parts of magnesium stearate, 6-8 parts of hydroxypropyl methyl cellulose, 10-15 parts of starch, 5-10 parts of vitamin C and 5-8 parts of vitamin B. The hamburger coffee Xiang Maicai freeze-drying powder contains acidic aspartic acid and glutamic acid, can better remove the straw smell of medicago leaf, straw smell of the medicago leaf can be eliminated, the medicago slices have refreshing and tasty taste, is popular by wide consumers, and better promotes the medicago leaf application.

The invention discloses a preparation method of an alfalfa leaf protein peptide beverage. The method includes adopting fresh bloom alfalfa leaves as raw materials, obtaining alfalfa liquid by the washing, shearing, beating and filtering process, extracting alfalfa leaf protein by the acid-heating method, preparing alfalfa leaf protein peptide powder by enzymolysis of the alfalfa leaf protein, and obtaining the alfalfa leaf protein peptide beverage by mixing the peptide powder, concentrated fruit juice, castor sugar, sour agent, beta-cyclodextrin, compound stabilizer, emulsifier and drinking water. The beverage includes, by weights: 5 to 10 parts of alfalfa leaf protein peptide powder, 10 to 20 parts of concentrated fruit juice, 5 to 10 parts of castor sugar, 1 to 5 parts of sour agent, 0.2 to 0.5 part of beta-cyclodextrin, 0.1 to 0.5 part of compound stabilizer, 0.05 to 0.1 part of emulsifier and the balance of drinking water. According to the method, easily-absorbed alfalfa leaf protein with the low molecular weight is adopted as the protein source, the concentrated fruit juice and other food additives are added, various nutrients of proteins and carbohydrates are provided, and various biologically active ingredients with the health caring functions are provided. Thus, the beverage has good taste and flavor, nutrients are rich, various physiological functions of anti-aging performance, body immunity enhance and digestion and absorption promotion are provided, and the beverage is adaptive to various people. The process is simple, the conditions are mild, energy consumption is low, the production period is short, the peptide preparation rate is high, the operationality is high, and the method is adaptive to large-scale industrial production.

The invention discloses a method for improving conjugational transfer efficiency of fluorescent plasmid transfer of fluorescence labeling strains of rhizobia by taking sterilizing alfalfa leaves as triparental mating carriers instead of sterile filter membranes. The method can be used for efficiently constructing CFP (cyan fluorescent protein) gene labeled strains of rhizobia. The method specifically comprises the following steps of taking small and tender leaves of alfalfa, uniformly putting the leaves in a single layer in a culture dish after washing the leaves with distilled water, and sterilizing the leaves; taking out the leaves with a pair of sterile tweezers, sticking the leaves to the surface of TY culture media, and covering the culture dish; and using the leaves to replace microporous filter membranes in the triparental mating process to carry out mixed growing culture on donors, assistant bacteria and recipient bacteria. Compared with the microporous filter membranes as the mating carriers, the alfalfa leaves, as the mating carriers, can improve the conjugational transfer efficiency of the fluorescent plasmids by 8.4-11 times, and the difference is obvious. As the used raw materials are alfalfa plant leaves which are renewable and accessible, the method has the advantage that the raw materials are cheap and accessible, is simple and convenient to operate and is suitable for constructing the fluorescence labeling strains of rhizobia.

The invention discloses a method for genetic transformation of purple alfalfa chloroplast. The method is characterized in that purple alfalfa leaves can be bombarded through a particle gun, the bombarded leaves are screened on a selective medium containing spectinomycin, the transgenic plant of the purple alfalfa chloroplast is obtained after three times screening. The invention is capable of providing a carrier and a method for chloroplast transformation of purple alfalfa and realizing the expression of green fluorescent protein in the purple alfalfa chloroplast.

The invention discloses milk powder feed for young livestock. The milk powder feed mainly comprises whey protein, casein, immune globulin, lecithin, milk powder AA (arachidonic acid), milk powder DHA (docosahexaenoic acid), inositol, fructo-oligosaccharide, lutein, saccharose, starch, alfalfa leaf protein powder, nucleotide, unsaturated fatty acid, crude fiber enzyme, multi-vitamin, multi-mineral and the like; the raw materials above are ultramicro-pulverized and mixed well to obtain the milk powder for young of large animals. The milk powder feed is mainly applied to young livestock, boosting appetite and meeting basic needs of the young livestock; clinically, the milk powder feed serves as a supplement in case that first-time female animals have no milk or have insufficient milk for the young; meanwhile, the milk powder feed further serves as an energy supplement in case of diseases occurring.

The invention discloses a composite formaldehyde scavenger containing thuja sutchuenensis essential oil. The composite formaldehyde scavenger containing thuja sutchuenensis essential oil comprises thefollowing raw materials in parts by weight: 10-20 parts of soybean protein, 20-40 parts of plant leaf protein, 1-10 parts of a compound essential oil, 0.2-1 part of sodium hypochlorite, 0.2-1 part ofprotease, 0.2-1 part of activated carbon, 1-5 parts of a nano antibacterial agent and 50-80 parts of purified water; wherein the compound essential oil is a mixture of thuja sutchuenensis essential oil, mint essential oil and thyme essential oil in a mass ratio of 2-4: 1: 1; the plant leaf protein is one or two selected from alfalfa leaf protein and pumpkin leaf protein. The invention also discloses a preparation method of the composite formaldehyde scavenger containing thuja sutchuenensis essential oil. The composite formaldehyde scavenger containing thuja sutchuenensis essential oil can actively adsorb, absorb and capture formaldehyde, and then react with formaldehyde, the problem of indoor air pollution is well solved, meanwhile, immune protein increase can be promoted, breathing and central nervous system balance can be effectively adjusted, oral cavity and throat mucous membranes are protected, and growth of various tumor cells is inhibited.

The invention discloses a promoter sequence with photoinduction specificity and leaf tissue specificity for promoting expression of alfalfa plastocyanin and a pair of primers for quickly cloning the promoter. The document reports that the plastocyanin is a plastid protein related to plant photosynthesis, and has been proved to be a photoinduction expression protein. The alfalfa plastocyanin promoter has 9 specific elements, including TATAbox, CAATbox and other promoter necessary elements, a specific element G-box(ctttatca) related to photoresponse, and GT-1 basic sequence (aatccaca) and the like related to tissue specificity expression; and the experiment proves that the promoter has certain photoinduction specificity and leaf tissue specificity, and can drive the specific expression of the reported gene GUS in transgenic alfalfa leaf. The primers are specific primers designed in reference to related sequences of Medicago truncatula genome, and are suitable for quickly cloning the alfalfa plastocyanin promoter by a PCR (polymerase chain reaction) method.

The invention relates to a nutritional composition containing alfalfa leaf protein and a preparation method of the nutritional composition, and belongs to the field of health foods. The composition is prepared by the following raw materials by weight: 8 to 10 parts of alfalfa leaf protein, 18 to 20 parts of semen coicis, 18 to 20 parts of soybean, 8 to 12 parts of walnut, 9 to 12 parts of rhizoma dioscoreae, 8 to 10 parts of red dates, 8 to 10 parts of black sesame, 6 to 8 parts of osmanthus fragrans, 8 to 10 parts of konjac, and 8 to 10 parts of chestnut. The nutritional composition includes the alfalfa leaf protein, food ingredients of the alfalfa leaf protein and the like, containing a plurality of nutritional elements of amino acids, dietary fibers, vitamins and the like, the nutritional composition is rich in nutritional content and high in food therapy value; therefore, the purposes of balancing nutrition, maintaining body movement, conditioning bodies and preventing diseases can be achieved.

The invention relates to a nutritional composition containing alfalfa leaf protein and a preparation method of the nutritional composition, and belongs to the field of health foods. The composition is prepared by the following raw materials by weight: 8 to 10 parts of alfalfa leaf protein, 18 to 20 parts of semen coicis, 18 to 20 parts of soybean, 8 to 12 parts of walnut, 9 to 12 parts of rhizoma dioscoreae, 8 to 10 parts of red dates, 8 to 10 parts of black sesame, 6 to 8 parts of osmanthus fragrans, 8 to 10 parts of konjac, and 8 to 10 parts of chestnut. The nutritional composition includes the alfalfa leaf protein, food ingredients of the alfalfa leaf protein and the like, containing a plurality of nutritional elements of amino acids, dietary fibers, vitamins and the like, the nutritional composition is rich in nutritional content and high in food therapy value; therefore, the purposes of balancing nutrition, maintaining body movement, conditioning bodies and preventing diseases can be achieved.

The invention discloses a medicago truncatula leaf development regulatory gene STF, and encoding protein of the gene. The medicago truncatula leaf development regulatory gene has a nucleotide sequence shown as SEQ ID No.2 and an amino acid sequence shown as SEQ ID No.1. An overexpression medicago truncatula leaf development regulatory gene STF can obviously improve two important agronomic characters, namely leaves and stems, of monocotyledon crops (rice), STF gene overexpression rice material leaves are widened and thickened, the stems are thick, the photosynthetic efficiency and lodging-resistant capacity of the rice are remarkably improved, and the problems that the photosynthetic efficiency is low, the stems are weak, lodging easily occurs and the like of the monocotyledon crops such as the rice in agricultural production can be solved.

The invention relates to the technical field of a feed additive and particularly relates to a compound type pig feed additive as well as preparation and application methods thereof. The compound type pig feed additive is formed by the following components in parts by weight: 50-85 parts of eucommia ulmoides leaf powder, 10-30 parts of alfalfa leaf powder and 5-20 parts of folium callicarpae pedunculalae leaf powder. The compound type pig feed additive is prepared by taking fresh eucommia ulmoides leaves, alfalfa leaves and folium callicarpae pedunculalae leaves, which are not corroded by plant diseases and insect pests as raw materials, crushing the leaves into powder of being less than 0.5 mm after drying the leaves in vacuum, and mixing according to the mass ratios. The feed additive has the advantages that the immunity of live pigs is effectively improved, the growth of the live pigs is accelerated and the meat quality is improved.

The invention discloses a method for obtaining a water-soluble dietary fiber by fermenting alfalfa leaves through bacillus subtilis. The method includes the following steps that bacillus-subtilis culture liquid with the spore concentration of 107 CFU / ml to 108 CFU / ml is prepared; alfalfa leaf powder is added into nutrient liquid according to the solid-liquid ratio of 1g / 29 ml to 1g / 31 ml, sealingis conducted, then sterilization is conducted, and alfalfa liquid is obtained; under an aseptic condition, the bacillus-subtilis culture liquid is introduced into the alfalfa liquid, and fermentationcultivation is conducted in a shaking table; fermentation liquid is sterilized to be centrifuged; supernatant obtained through centrifugation is sucked and filtered, absolute ethyl alcohol is added into the obtained filter liquid, then standing and centrifugation are conducted, the obtained product is settled, frozen and dried, and the alfalfa water-soluble dietary fiber is obtained. The method iseasy to operate, short in used time and high in extraction rate.

The invention discloses a method for using sterilized alfalfa leaves instead of sterile filter membranes as a three-parent hybrid carrier to improve the efficiency of fluorescent plasmid transfer conjugation and transfer of rhizobia fluorescently labeled strains, which can be used to efficiently construct rhizobia CFP cyan fluorescent protein genes To mark the bacterial strain, the method is as follows: take the young leaves of alfalfa, wash them with distilled water, place the leaves evenly in a single layer in a petri dish, and sterilize them; take out the leaves with sterile tweezers, and stick them on the surface of TY medium , and cover the Petri dish. In the process of three-parent hybridization, instead of the microporous membrane, the mixed growth culture of donor bacteria, auxiliary bacteria and recipient bacteria is carried out. Compared with the microporous filter membrane as the hybrid carrier, using alfalfa leaves as the hybrid carrier can increase the conjugative transfer rate of fluorescent plasmids by 8.4-11 times, and the difference reaches a significant level; because the raw materials used are renewable and easily obtained alfalfa plants The leaves, raw materials are cheap and easy to obtain, and the operation is simple and convenient, which is suitable for the construction of rhizobia fluorescently labeled strains.

The invention discloses a chicken raising method for improving egg quality in summer. The method comprises the steps that 0.05% of choline, 0.05% of sunflower seed, 0.03% of paprika, 5% of sun dried and smashed alfalfa leaf are added by weight to a common chicken feed. The chicken raising method for improving egg quality in summer is capable of directly improving appetite of chicken in summer (chicken flocks have a poor appetite in summer and are reluctant to eat), the hen laying rate in summer is indirectly increased, the laid egg quality is improved, and therefore the yolk color gets darker and cholesterol in yolk is capable of decreasing by around10%.

The invention provides alfalfa antioxidant glycoprotein as well as a preparation method and application thereof, belonging to the technical field of protein modification. According to the invention, alfalfa leaf protein, reducing sugar and water are mixed and then subjected to a damp and hot Maillard reaction, and the alfalfa antioxidant glycoprotein is prepared. When a pH value is 9, a substrate ratio is 1: 3, reaction temperature is 80 DEG C and reaction time is 30 minutes, the antioxidant activity of the alfalfa antioxidant glycoprotein is most remarkable, total reducing capacity reaches 1.25, a DPPH. clearance rate reaches 47.55%, and a HO. clearance rate reaches 20.49%. By utilizing the method disclosed by the invention, the application range of the clover leaf protein can be obviously widened, and a reference basis is provided for resource development and utilization of the clover leaf protein, application of the clover leaf protein in food industry and development of novel and natural antioxidants.

The invention discloses an alfalfa leaf type flyover crossing structure which comprises a first main trunk road and a second main trunk road, wherein the first main trunk road and the second main trunk road cross. Turn-right ramps are arranged in four quadrants formed by the first main trunk road and the second main trunk road respectively. The alfalfa leaf type flyover crossing structure further comprises four turn-left ramps, and each turn-left ramp stretches over two adjacent quadrants. Each turn-left ramp comprises a turn-left circuitous section and a turn-left annular section; the entrance of each turn-left circuitous section is communicated with the corresponding turn-right ramp where left turning vehicles are driven away, and the exit of corresponding turn-left annular section is communicated with a target lane of the left turning vehicles; each turn-left circuitous section is located on the outer side of the turn-left annular section located in the same quadrant with the turn-left circuitous section. According to the alfalfa leaf type flyover crossing structure, collector-distributor lanes do not need to be arranged; no intersecting section exists between the entrance and the exit of each turn-left ramp, and the traffic capacity of the ramps and mainlines is improved; the alfalfa leaf type flyover crossing structure is slightly lower than an alfalfa leaf type flyover crossing structure provided with collector-distributor lanes in the aspects of land occupancy, building height and project building cost.

The invention relates to a nutritional beer with green tea taste and a brewing method thereof, belonging to the field of food processing. The added parts of green tea powder and alfalfa leaf protein are: 15-20 parts and 5-10 parts respectively. Green tea powder, alfalfa leaf protein and wort are mixed and fermented with high-activity dry yeast. The beer obtained from fermentation has green tea aroma Strong smell, balance the bitterness produced by hop fermentation, alfalfa leaf protein is rich in essential amino acids, and the alcohol content is lower than that without green tea powder and alfalfa leaf protein. The invention solves the problems of single taste and high alcohol content in the existing beer. The invention adds alfalfa leaf protein to the beer, which not only expands the application range of the alfalfa leaf protein, but also makes the beer a nutritional and health product rich in protein.

The invention discloses a preparation method of an alfalfa leaf protein peptide beverage. The method includes: using fresh alfalfa leaves as raw materials, obtaining alfalfa juice through processes such as washing, cutting, beating, and filtering; extracting alfalfa leaf protein by acid-heating method; preparing alfalfa leaf protein peptide powder by enzymatically hydrolyzing alfalfa leaf protein; The peptide powder is mixed with concentrated apple juice, white sugar, citric acid, β-cyclodextrin, compound stabilizer, drinking water, etc. to obtain the alfalfa leaf protein peptide beverage. The beverage is calculated in parts by mass, including 5-10 parts of alfalfa leaf protein peptide powder, 10-20 parts of concentrated fruit juice, 5-10 parts of white sugar, 1-5 parts of sour agent, 0.2-0.5 parts of β-cyclodextrin, compound 0.1-0.5 part of stabilizer, 0.05-0.1 part of sucrose fatty acid ester, and the balance is drinking water. The preparation method of the beverage provided by the present invention uses low-molecular-weight, easily absorbed alfalfa leaf protein peptide as the protein source, supplemented with concentrated fruit juice and other food additives, not only contains various nutrients such as protein and sugar, but also contains A variety of bioactive ingredients with health functions. Therefore, the alfalfa leaf protein peptide beverage provided by the present invention has excellent taste and flavor, is rich in nutrition, and has multiple physiological functions such as anti-aging, enhancing body immunity, and promoting digestion and absorption, and is suitable for drinking by various groups of people. The invention has simple process, mild conditions, low energy consumption, short production cycle, high peptide yield and strong operability, and is suitable for large-scale industrial production.

The invention discloses a method for extracting alfalfa leaf protein by an ultrasonic-assisted enzyme process, belonging to the technical field of vegetable proteins. The method comprises the following steps: cleaning alfalfa leaves in early flowering stage as the raw material, pulping, and filtering to obtain a juice and pulp dregs; treating the juice by an ultrasonic-assisted enzyme process; adding cellulase into the juice while carrying out ultrasonic extraction to obtain the extracting solution, wherein 3-4g of cellulase is added to every 100ml of juice, the ultrasonic power is 100-250W, the ultrasonic frequency is 20-40kHz, the temperature is 50-55 DEG C, and the holding time of the ultrasonic action is 30-40 minutes; inactivating the enzyme, and flocculating the solution; carrying out continual treatment under the 4000-5000 r / min centrifugal action for 15-20 minutes to obtain a leaf protein crude extract and a supernatant; and extracting the leaf protein crude extract, and drying to constant weight, thereby obtaining the alfalfa leaf protein product. The invention has the advantages of simple technical process, mild conditions, low energy consumption, short extraction time and high extraction rate, is easy to operate and control, and is convenient for large-scale industrial production.

The invention discloses a decoloring method for antioxidant peptide of alfalfa leaf protein, and belongs to the technical field of decoloring for foods. The decoloring method comprises the following steps of adjusting the pH of an antioxidant peptide solution from alfalfa leaf protein, which is prepared through alkali protease, to 2-4, adding a mixture of powdered activated carbon, floridin and alkaline kaolin, to obtain a decolorizer, wherein the addition quantity of the composite decolorizer is 1.0-3.0%(m / V) of the antioxidant peptide solution of the alfalfa leaf protein, performing water-bath vibration for decoloring at 50-70 DEG C for 60-80min, and performing continuous treatment at the centrifugation of 4000-5000 r / min for 20-30min so as to obtain supernatant namely the decolored antioxidant peptide from the alfalfa leaf albumen. The decoloring method disclosed by the invention is simple in technological process, easy and feasible to operate, mild in treatment condition, and low in energy consumption; and besides, high decoloring rate is guaranteed, the antioxidative activity is also effectively reserved, and the decoloring method can be widely applied to the industry of foods produced through alfalfa polypeptide and other bioactive peptide.