Extraction method of alfalfa leaf protein

A technology of alfalfa leaf protein and extraction method, which is applied to the preparation method of peptides, chemical instruments and methods, and methods based on microorganisms. It can solve the problems of high energy consumption, large solvent consumption, and energy consumption in heating methods, and achieve energy efficiency. Low consumption, simple process, and the effect of avoiding pollution

Inactive Publication Date: 2009-02-11
BEIJING FORESTRY UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] (1) The heating method consumes a lot of energy, and in the process of slowly heating up, it is easy to cause a large amount of protein to suffer loss due to enzymatic hydrolysis;
[0004] (2) Both the acid method and the acid heat method have acid pollution, which makes the obtained leaf protein odor, color change, poor, and causes the problem that the digestibility of the cytoplasmic protein components in the leaf protein is reduced, and the acid heat method still exists The disadvantage of consuming energy;
[0005] (3) Alkaline method and alkali-thermal method all have the problem that the extracted leaf protein contains a large amount of alkaline chemical substances, has an unpleasant

Method used

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  • Extraction method of alfalfa leaf protein
  • Extraction method of alfalfa leaf protein
  • Extraction method of alfalfa leaf protein

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Embodiment 1

[0031] This embodiment provides a method for extracting alfalfa leaf protein, mainly extracting leaf protein from alfalfa leaf pulp by fermentation, such as figure 1 As shown, the method specifically includes:

[0032] The raw material is the alfalfa leaves of alfalfa in the budding stage. Generally, the budding stage refers to the fresh alfalfa in the second budding stage, or the fresh alfalfa in the third budding stage, or the fresh alfalfa in the second budding stage and the first crop. For fresh alfalfa in the budding period of three batches, the raw material of the alfalfa leaf is cleaned with tap water, and the withered, yellowed, rotten and pest-infested leaves in the raw material of the alfalfa leaf are removed by cleaning, and the soil, dust and some parasites on the surface of the alfalfa leaf are cleaned. After cleaning, the alfalfa leaves are beaten. Generally, the alfalfa leaves and water accounting for 90% to 95% of the weight of the alfalfa leaves are put into a...

Embodiment 2

[0037] The present embodiment provides a kind of alfalfa leaf protein extraction method, the alfalfa that selects is the fresh alfalfa of the 2nd stalk budding period and the 3rd vegetative period fresh alfalfa, obtain 2382.5 kilograms of alfalfa fresh grass after harvesting, wherein blade and young stem and leaf, 1,186.75 kg of flowers and 1,195.75 kg of stems; harvest about 655 kg of fresh alfalfa in the second budding stage, about 986.5 kg of fresh alfalfa in the second budding stage, and harvest fresh alfalfa in the third vegetative growth period Grass about 741 kg;

[0038] In order to avoid pollution, the alfalfa leaves separated from the stalks need to be cleaned to remove the withered, yellowed, rotten and pest-infested leaves, the soil, dust and some parasitic bacteria on the surface of the leaves are cleaned, and non-polluting disinfection measures are taken , to further kill microorganisms; beat the cleaned and sterilized alfalfa leaves using HR2839 / AC Phillips beat...

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Abstract

The invention discloses a method for extracting cloverleaf protein, which comprises that the cloverleaf of clover at squaring stage is taken as raw material and is pulped after being washed; the pulped cloverleaf slurry is filtered to obtain slurry liquid and filter residue; the obtained slurry liquid is treated by fermentation, lactic acid bacillus is adopted as zymolytic strain, the dosage of inoculation is 10<7>-10<9> of lactic acid bacillus viable cell used in every milliliter of the slurry liquid, the fermentation temperature is 30-39 DEG C, and the fermentation time lasts from 8 to 10 hours; the product which is treated by fermentation is continuously processed for 8-12min under the 300r/min of centrifugal effect, and crude extract of leaf protein and the supernatant fluid are obtained by flocculation after treatment; the crude extract of leaf protein is extracted and is treated by freeze drying, and cloverleaf protein products are obtained after the treatment. The method has simple technique and high content of extracted leaf protein; as heating is not needed, energy consumption is low; furthermore, acid, alkali or organic solvent, and the like are not needed to add, the obtained leaf protein is avoided being polluted.

Description

technical field [0001] The invention relates to a leaf protein extraction technology, in particular to a method for extracting alfalfa leaf protein. Background technology three [0002] Alfalfa Leaves Protein (ALP) refers to the crude protein product extracted from alfalfa leaves, mainly nitrogenous substances such as chloroplast protein, cytoplasmic protein and nucleic acid, and also includes carbohydrates, fatty acids, vitamins and minerals, etc. Composition. At present, the extraction process of leaf protein is mainly based on laboratory scale, including heating method, acid method, alkali method, acid heat method, alkali heat method, solvent method and ultrafiltration method. However, each method has certain deficiencies, as follows: [0003] (1) The heating method consumes a lot of energy, and in the process of slowly heating up, it is easy to cause a large amount of protein to suffer loss due to enzymatic hydrolysis; [0004] (2) Both the acid method and the acid he...

Claims

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Application Information

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IPC IPC(8): C12P21/00C07K1/14C12R1/225
Inventor 卢欣石王峰李魁英王铁梅冯长松莫本田
Owner BEIJING FORESTRY UNIVERSITY
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