Extraction method of alfalfa leaf protein

Abstract

The invention discloses a method for extracting cloverleaf protein, which comprises that the cloverleaf of clover at squaring stage is taken as raw material and is pulped after being washed; the pulped cloverleaf slurry is filtered to obtain slurry liquid and filter residue; the obtained slurry liquid is treated by fermentation, lactic acid bacillus is adopted as zymolytic strain, the dosage of inoculation is 10<7>-10<9> of lactic acid bacillus viable cell used in every milliliter of the slurry liquid, the fermentation temperature is 30-39 DEG C, and the fermentation time lasts from 8 to 10 hours; the product which is treated by fermentation is continuously processed for 8-12min under the 300r / min of centrifugal effect, and crude extract of leaf protein and the supernatant fluid are obtained by flocculation after treatment; the crude extract of leaf protein is extracted and is treated by freeze drying, and cloverleaf protein products are obtained after the treatment. The method has simple technique and high content of extracted leaf protein; as heating is not needed, energy consumption is low; furthermore, acid, alkali or organic solvent, and the like are not needed to add, the obtained leaf protein is avoided being polluted.

Description

technical field [0001] The invention relates to a leaf protein extraction technology, in particular to a method for extracting alfalfa leaf protein. Background technology three [0002] Alfalfa Leaves Protein (ALP) refers to the crude protein product extracted from alfalfa leaves, mainly nitrogenous substances such as chloroplast protein, cytoplasmic protein and nucleic acid, and also includes carbohydrates, fatty acids, vitamins and minerals, etc. Composition. At present, the extraction process of leaf protein is mainly based on laboratory scale, including heating method, acid method, alkali method, acid heat method, alkali heat method, solvent method and ultrafiltration method. However, each method has certain deficiencies, as follows: [0003] (1) The heating method consumes a lot of energy, and in the process of slowly heating up, it is easy to cause a large amount of protein to suffer loss due to enzymatic hydrolysis; [0004] (2) Both the acid method and the acid he...

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Problems solved by technology

[0003] (1) The heating method consumes a lot of energy, and in the process of slowly heating up, it is easy to cause a large amount of protein to suffer loss due to enzymatic hydrolysis;

[0004] (2) Both the acid method and the acid heat method have acid pollution, which makes the obtained leaf protein odor, color change, poor, and causes the problem that the digestibility of the cytoplasmic protein components in the leaf protein is reduced, and the acid heat method still exists The disadvantage of consuming energy;

[0005] (3) Alkaline method and alkali-thermal method all have the problem that the extracted leaf protein contains a large amount of alkaline chemical substances, has an unpleasant smell, and is brown in color, and the follow-up use has to go through the problems of alkali removal, deodorization and decolorization; And the alkaline thermal method also has the disadvantage of consuming energy;

[0006] (4) The solvent method has the disadvantages of excessive solvent consumption in the extraction process, residual organic solvent and loss of part of the pigment, and poor color of the product;

[0007] (5) Although the ultrafiltration method can obtain products that maintain the color and smell of the alfalfa grassland, the expensive ultrafiltration equipment is the main obstacle to the popularization of this process, resulting in a corresponding increase in production costs

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