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Method for obtaining water-soluble dietary fiber by fermenting alfalfa leaves through bacillus subtilis

A technology of Bacillus subtilis and alfalfa, applied in the food field, can solve the problem of low extraction rate of water-soluble dietary fiber, and achieve the effect of short time and simple operation

Pending Publication Date: 2019-07-12
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The water-soluble dietary fiber extraction rate of this method is low, only about 7%

Method used

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  • Method for obtaining water-soluble dietary fiber by fermenting alfalfa leaves through bacillus subtilis
  • Method for obtaining water-soluble dietary fiber by fermenting alfalfa leaves through bacillus subtilis
  • Method for obtaining water-soluble dietary fiber by fermenting alfalfa leaves through bacillus subtilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, a kind of method of Bacillus subtilis fermentation alfalfa leaf obtains water-soluble dietary fiber, carries out following steps successively:

[0052] 1), prepare spore concentration to be 10 7 -10 8 The Bacillus subtilis culture fluid of CFU / ml;

[0053] Weigh 90g of nutrient broth medium, add 500ml of distilled water, stir until completely dissolved, and sterilize (autoclave at 121°C for 20min) to obtain a liquid medium;

[0054] In a sterile environment, use a pipette gun to draw 0.1ml bacterial glycerin solution, add it to a 250ml Erlenmeyer flask containing 100ml liquid medium, and cultivate it in a shaker at 30°C with a shaking frequency of 130r / min until the spore concentration is about for 10 8 CFU / ml Bacillus subtilis culture solution, the culture time is about 36h.

[0055] Nutrient broth medium can be purchased from Hangzhou Microbial Reagent Co., Ltd.

[0056]Bacillus subtilis is the Bacillus subtilis number 1.14985 of China General Micro...

Embodiment 2

[0065] Embodiment 2, the inoculum amount of embodiment 1 step 5) is changed into 0.1%, 0.5%, 2%, 4%, 6%, 8%, 10% respectively by 1%, all the other are equal to embodiment 1; Gained extraction rate as figure 1 mentioned.

Embodiment 3

[0066] Embodiment 3, the solid-liquid ratio of embodiment 1 step 4) is changed into 1g / 10ml, 1g / 15ml, 1g / 20ml, 1g / 40ml, 1g / 50ml respectively by 1g / 30ml; All the other are equal to embodiment 1; Gained The extraction rate as figure 2 mentioned.

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Abstract

The invention discloses a method for obtaining a water-soluble dietary fiber by fermenting alfalfa leaves through bacillus subtilis. The method includes the following steps that bacillus-subtilis culture liquid with the spore concentration of 107 CFU / ml to 108 CFU / ml is prepared; alfalfa leaf powder is added into nutrient liquid according to the solid-liquid ratio of 1g / 29 ml to 1g / 31 ml, sealingis conducted, then sterilization is conducted, and alfalfa liquid is obtained; under an aseptic condition, the bacillus-subtilis culture liquid is introduced into the alfalfa liquid, and fermentationcultivation is conducted in a shaking table; fermentation liquid is sterilized to be centrifuged; supernatant obtained through centrifugation is sucked and filtered, absolute ethyl alcohol is added into the obtained filter liquid, then standing and centrifugation are conducted, the obtained product is settled, frozen and dried, and the alfalfa water-soluble dietary fiber is obtained. The method iseasy to operate, short in used time and high in extraction rate.

Description

technical field [0001] The invention belongs to the field of food, and in particular relates to a method for obtaining water-soluble dietary fiber by fermenting alfalfa leaves with bacillus subtilis. Background technique [0002] Common water-soluble dietary fibers mainly include: inulin, dextran, resistant starch, chitosan, oat β-glucan, guar gum, sodium alginate, fungal polysaccharides, etc. Barley, Foods such as beans, carrots, citrus, flax, oats and oat bran are rich in soluble fiber. In contrast, water-soluble dietary fiber has been widely used as a thickener, bulking agent, formulation aid and filler in food processing due to its good processing performance and better physiological functions. The production and development of food and functional food, and the related food of water-based dietary fiber have huge development space and broad market prospect. [0003] Medicago sativa (scientific name: Medicago sativa.) is a perennial herb of the leguminous family and alfa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/21
CPCA23L33/21
Inventor 于海宁吴漫漫沈生荣
Owner ZHEJIANG UNIV OF TECH
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