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Preparation method of alfalfa leaf protein peptide beverage

An alfalfa leaf protein and beverage technology, applied in food preparation, food science, application and other directions, can solve the problems of resource waste and lack of plant protein resources, and achieve the effect of high specificity, easier digestion and absorption, and easy digestion and absorption.

Active Publication Date: 2015-09-23
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of the shortage of plant protein resources in my country and the waste of resources caused by a large amount of high-quality forage such as alfalfa being only used as feed, the embodiment of the present invention provides a method for preparing alfalfa leaf protein peptide beverage, that is, using a biological enzyme method to prepare alfalfa leaf protein peptide , and supplemented with concentrated fruit juice and various food additives to prepare alfalfa leaf protein peptide beverage with excellent taste, flavor, rich nutrition, anti-aging, enhancing body immunity, promoting digestion and absorption, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of alfalfa leaf protein peptide drink, the steps of the preparation method are:

[0029] (1) Using fresh alfalfa leaves at the budding stage as raw materials, alfalfa juice is obtained through processes such as washing, cutting, beating, and filtering;

[0030] (2) Place the obtained alfalfa juice in a constant temperature water bath shaker at a temperature of 50 °C for heat preservation treatment. The speed of the water bath shaker is 150 r / min. While shaking, add 1 mol / L hydrochloric acid solution dropwise to adjust the pH of the alfalfa juice When the value reaches the isoelectric point (4.0), the alfalfa leaf protein is precipitated. After centrifuging at 4000 r / min for 30 min, the supernatant is discarded, and the precipitated alfalfa leaf protein is freeze-dried at a temperature of - Pre-freeze in a low-temperature refrigerator at 60°C for 12 hours, and then transfer to a freeze dryer for 48 hours to freeze-dry to obtain alfalfa leaf protein f...

Embodiment 2

[0035] A preparation method of alfalfa leaf protein peptide drink, the steps of the preparation method are:

[0036] (1) Same as embodiment 1;

[0037] (2) Place the obtained alfalfa juice in a constant temperature water bath shaker at 50 °C for heat preservation treatment. The speed of the water bath shaker is 200 r / min. While shaking, add 1 mol / L hydrochloric acid solution dropwise to adjust the pH of the alfalfa juice When the value reaches the isoelectric point (4.0), the alfalfa leaf protein is precipitated. After centrifuging at 4000 r / min for 30 min, the supernatant is discarded, and the precipitated alfalfa leaf protein is freeze-dried at a temperature of - Pre-freeze in a low-temperature refrigerator at 60°C for 12 hours, and then transfer to a freeze dryer for 48 hours to freeze-dry to obtain alfalfa leaf protein freeze-dried powder;

[0038] (3) The obtained alfalfa leaf protein freeze-dried powder was made into an alfalfa leaf protein solution with a mass concentr...

Embodiment 3

[0042] A preparation method of alfalfa leaf protein peptide drink, the steps of the preparation method are:

[0043] (1) Same as embodiment 1;

[0044] (2) Place the obtained alfalfa juice in a constant temperature water bath shaker at 50 °C for heat preservation treatment. The speed of the water bath shaker is 200 r / min. While shaking, add 1 mol / L hydrochloric acid solution dropwise to adjust the pH of the alfalfa juice When the value reaches the isoelectric point (4.0), the alfalfa leaf protein is precipitated. After centrifuging at 4000 r / min for 30 min, the supernatant is discarded, and the precipitated alfalfa leaf protein is freeze-dried at a temperature of - Pre-freeze in a low-temperature refrigerator at 60°C for 12 hours, and then transfer to a freeze dryer for 48 hours to freeze-dry to obtain alfalfa leaf protein freeze-dried powder;

[0045] (3) The obtained alfalfa leaf protein freeze-dried powder was made into an alfalfa leaf protein solution with a mass concentr...

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PUM

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Abstract

The invention discloses a preparation method of an alfalfa leaf protein peptide beverage. The method includes adopting fresh bloom alfalfa leaves as raw materials, obtaining alfalfa liquid by the washing, shearing, beating and filtering process, extracting alfalfa leaf protein by the acid-heating method, preparing alfalfa leaf protein peptide powder by enzymolysis of the alfalfa leaf protein, and obtaining the alfalfa leaf protein peptide beverage by mixing the peptide powder, concentrated fruit juice, castor sugar, sour agent, beta-cyclodextrin, compound stabilizer, emulsifier and drinking water. The beverage includes, by weights: 5 to 10 parts of alfalfa leaf protein peptide powder, 10 to 20 parts of concentrated fruit juice, 5 to 10 parts of castor sugar, 1 to 5 parts of sour agent, 0.2 to 0.5 part of beta-cyclodextrin, 0.1 to 0.5 part of compound stabilizer, 0.05 to 0.1 part of emulsifier and the balance of drinking water. According to the method, easily-absorbed alfalfa leaf protein with the low molecular weight is adopted as the protein source, the concentrated fruit juice and other food additives are added, various nutrients of proteins and carbohydrates are provided, and various biologically active ingredients with the health caring functions are provided. Thus, the beverage has good taste and flavor, nutrients are rich, various physiological functions of anti-aging performance, body immunity enhance and digestion and absorption promotion are provided, and the beverage is adaptive to various people. The process is simple, the conditions are mild, energy consumption is low, the production period is short, the peptide preparation rate is high, the operationality is high, and the method is adaptive to large-scale industrial production.

Description

technical field [0001] The invention relates to a functional beverage, in particular to a preparation method of an alfalfa leaf protein peptide beverage. Specifically, the invention relates to a method for producing alfalfa leaf protein peptide beverage by adopting an enzymatic hydrolysis method, and belongs to the field of food. Background technique [0002] Alfalfa leaf protein peptide is a polymer of several, dozens or even hundreds of amino acids linked by peptide bonds, which is produced after hydrolysis of alfalfa leaf protein by protease. In recent years, many scholars have studied the digestion and absorption mechanism of peptides. They believe that peptides are more easily absorbed by the intestine than proteins and free amino acids. Biological metabolism experiments have confirmed that the absorption rate of peptides is greater than that of free amino acids, and it is easier and faster than amino acids to pass through. Small intestinal mucosa to be absorbed and u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L1/29A23L1/305A23L1/015
CPCA23L2/38A23V2002/00A23V2250/548A23V2250/5112A23V2200/222A23V2300/28
Inventor 许英一吴红艳黄新育林巍孙长豹
Owner QIQIHAR UNIVERSITY
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