26 results about "Rhodococcus sp." patented technology

To provide a technique for decomposing hydrocarbon compounds in soil and / or water, using microorganisms with hydrocarbon-decomposing capabilities even under anaerobic environments and low safety risks.SOLUTION: The present invention provides a method for decomposing hydrocarbon compounds in soil and / or water using microorganisms, the method comprising a step of contacting the soil and / or water containing the hydrocarbon compounds with the microorganisms. The microorganisms refer to one or more microorganisms belonging to any of the species of (1) Gordonia neofelifaecis, (2) Gordonia rubripertincta, and (3) Rhodococcus rhodochrous.SELECTED DRAWING: None

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The invention discloses rhodococcus and application thereof in biodegradation of octogen, and belongs to the technical field of microorganisms. The invention aims to provide a strain of rhodococcus capable of degrading oversaturated high-concentration octogen. The invention provides a strain of Rhodococcus sp. LH-2, the name of the Rhodococcus sp. LH-2 is Rhodococcus sp. LH-2, the Rhodococcus sp. LH-2 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 15, 2024, and the preservation number is CGMCC No.31308. The invention also provides a method for preparing the Rhodococcus sp. LH-2. The Rhodococcus sp.LH-2 used in the invention can degrade saturated high-concentration octogen, which indicates that the Rhodococcus sp.LH-2 has a certain application prospect in the resource utilization of octogen wastewater.

The present invention discloses a composite microbial agent and its preparation method and application. The composite microbial agent includes bacterial strains, and the bacterial strains include Pseudomonas, Rhodococcus, and Bacillus. The Pseudomonas includes at least one of Pseudomonas sp. Z07 with a preservation number of CCTCC NO: M 20241294 and Pseudomonas sp. ZD1 with a preservation number of CCTCC NO: M 20241560. The Rhodococcus includes Rhodococcus sp. ZS1D-28 with a preservation number of CCTCC NO: M 2023986. The Bacillus includes Bacillus velezensis XN1 with a preservation number of CCTCC NO: M 2024857. Using the composite microbial agent of the present invention for the pollution remediation of slightly polluted water bodies in plain river network areas can grow and degrade nitrogen and phosphorus pollution in water bodies under a low-nutrient environment without adding an external carbon source or with less addition of a carbon source, and has the advantages of high efficiency, simplicity, and being conducive to popularization and application.

The present application relates to the field of microbial technology, in particular to a strain of Rhodococcus sp YH-1, its application and aromatic hydrocarbon pollution degradation agent, the Rhodococcus sp YH-1 is preserved in China typical culture preservation center on April 30, 2024, and the preservation number is CCTCC NO:M2024845.The Rhodococcus sp YH-1 of the present application has good degradation performance to benzene, toluene, chlorobenzene and o-dichlorobenzene.When degrading for 96h, the degradation rate of Rhodococcus sp YH-1 to 1000mg / L toluene, chlorobenzene and o-dichlorobenzene all reaches more than 50%;The degradation rate to benzene with concentration of 20-1000mg / L reaches more than 90%.The results show that Rhodococcus sp YH-1 has good degradation effect to aromatic hydrocarbon, and it has high practical application value and broad development prospect in repairing aromatic hydrocarbon contaminated soil, purifying aromatic hydrocarbon contaminated air and treating aromatic hydrocarbon wastewater.

Bacterial strains PG42 and NH42 are obtained through primary screening from alpine forest litters and soil, bacterial colonial morphology is combined to obtain bacteria, bacterial strains with multiple physiological activities are screened through physiological and biochemical experiments, DNA is extracted from the bacterial strains, 16S rRNA is amplified, and sequence alignment is carried out; the preparation method comprises the following steps: screening a microbial inoculum, namely activating bacillus subtilis MZS1 (B.sub MZS1) and rhodococcus PG42 (Rhodococcus sp.PG42) according to a ratio of 2: 1; and preparing the microbial inoculum. The microbial agent is applied to grass seed screening, the screening efficiency is high, the acting speed is high, the effect of replacing chemical fertilizer is achieved, and pollution to the environment is reduced.

The invention relates to the technical field of enzyme engineering, synthetic biology and microbial fermentation, in particular to an amide hydrolase mutant and application thereof. The substrate range of the amide hydrolase RsAD from the microorganism Rhodococcus sp. Is relatively narrow, and after the amino acid residue L447 in the substrate channel of the amide hydrolase RsAD is mutated into alanine, the reaction enzyme activity of the alanine on the large-volume substrate, namely indoleacetamide, is increased, so that the production efficiency of indoleacetic acid is greatly improved.

The invention relates to the technical field of environmental microorganisms, in particular to Rhodococcus ruber CPU-RR2502 with a strong nitrite degradation function, the strain is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.35890. The invention further discloses a preparation method of the Rhodococcus ruber CPU-RR2502 with the strong nitrite degradation function. The strain is separated from bottom mud of an aquaculture pond and has efficient degradation capacity on nitrite in a water body, and the degradation rate on 1g / L sodium nitrite within 72 hours reaches 96.8%. The strain can adapt to an aquaculture environment, can be used for developing a microbial preparation, greenly and safely controlling the nitrite concentration through a bioremediation mode, ensuring the safety of aquatic animals and reducing the risk of secondary pollution caused by a traditional chemical method, and has a wide application prospect.

To promote the growth of microalgae.SOLUTION: The present invention provides a microorganism belonging to any genus selected from Rhodococcus, Xanthobacter, Ancylobacter, Shewanella, or Aeromonas. The microorganism may be Rhodococcus cerastii of the genus Rhodococcus, Xanthobacter flavus of the genus Xanthobacter, or Ancylobacter rudongensis of the genus Ancylobacter. The microorganism may be intended for promoting the growth of cyanobacteria, green algae, gray algae or euglenoids.SELECTED DRAWING: Figure 4

The invention discloses a method for immobilizing rhodococcus and application thereof, and belongs to the technical field of microbial immobilization. The invention aims to provide a rhodococcus which has an immobilization effect and can efficiently and cyclically degrade hexogen and octogen and a method for cyclically utilizing the rhodococcus. The invention provides a method for immobilizing Rhodococcus sp.. The method is characterized in that the immobilized Rhodococcus sp. Is obtained from a bacterial solution of Rhodococcus LH-2, hydroxyl-terminated polybutadiene rubber blocks and Rhodococcus LH-2 according to a mixing ratio of 0.5 cm < 3 > * (10-20) / 200ml, or obtained from a bacterial solution of polyurethane foam and Rhodococcus LH-2 according to a mixing ratio of 1 cm < 3 > * (5-15) / 200ml, and the immobilized Rhodococcus sp. Is obtained from the bacterial solution of polyurethane foam and Rhodococcus LH-2 according to a mixing ratio of 1 cm < 3 > * (5-15) / 200ml. The immobilized microorganism technology is utilized, hydroxyl-terminated polybutadiene is used as an immobilized carrier, and free rhodococcus can improve the self activity and stability, so that a theoretical basis and a data support are provided for practical application and bioremediation of the rhodococcus.

The invention belongs to the field of sewage treatment, and particularly relates to a complex microbial inoculant for rural domestic sewage treatment and application of the complex microbial inoculant. The composite bacterial agent is prepared from at least two of the following bacterial strains: Rhodococcus sp. 24, Rhodococcus sp. YB and Pseudomonas tianjinensis sp., and the composite bacterial agent is prepared from at least two of the following bacterial strains: Rhodococcus sp. 24, Rhodococcus sp. YB and Pseudomonas tianjinensis sp. The complex microbial inoculant can efficiently remove ammonia nitrogen and organic pollutants in rural black grey water treatment, is high in adaptability and stable in treatment effect, and has a wide application prospect. The invention provides a new solution for rural sewage treatment, and has important environmental and economic benefits.

The invention relates to the technical field of microorganisms, and particularly discloses a multifunctional microbial agent as well as a preparation method and application thereof, and the technical key point is that the microbial agent comprises bacillus subtilis MZS1 (B.sub MZS1), rhodococcus PG42 (Rhodococcus sp.PG42) and an auxiliary synergist. The auxiliary synergist is an amino acid compound, and the amino acid compound contains 9% of glutamic acid, 3.5% of proline, 6.5% of glycine, 7% of alanine, 7% of valine, 0.5% of methionine, 0.5% of isoleucine, 9% of leucine, 2% of tyrosine, 5% of phenylalanine, 8% of lysine, 6% of histidine and 3% of arginine. According to the preparation method of the multifunctional microbial agent and the using technology of the multifunctional microbial agent in the alpine degraded grassland, the problems that in the prior art, the using cost is high, the using effect is unknown, and the ecological restoration development requirement of the alpine environment cannot be met are solved.

The invention belongs to the technical field of estrogen degradation, and particularly discloses an application of low-temperature-resistant SDR dehydrogenase in estradiol degradation, and the low-temperature-resistant SDR dehydrogenase RQCSRS03565 is obtained by screening from Rhodococcus RCBS9, expressing in Escherichia coli and purifying. According to the invention, a novel SDR dehydrogenase RQCSRS03565 is identified and functionally verified from a rhodococcus strain RCBS9 for the first time, and the novel SDR dehydrogenase RQCSRS03565 has the characteristic of low temperature resistance and can still efficiently degrade E2 at a low temperature of 10 DEG C. Through structural simulation and site-directed mutagenesis experiments, the SER-205 is identified as a key functional residue of the enzyme for catalyzing estradiol degradation for the first time. Discovery of the low-temperature-resistant degrading enzyme and identification of key residues of the low-temperature-resistant degrading enzyme provide core elements and theoretical bases for development of efficient estradiol bioremediation technologies and preparations suitable for low-temperature environments, and the low-temperature-resistant degrading enzyme has important application value.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to rhodococcus and application thereof. The strain is Rhodococcus qingshenii (short for strain R), the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 13, 2025, and the preservation number of the strain is CGMCC No. 34527. The invention further discloses a preparation method of the Rhodococcus qingshenii strain. The strain has high-efficiency fermentation iron reduction capacity at low temperature (4 DEG C) and under strict anaerobic conditions, and can be applied to remediation of polluted sites in cold regions, especially deep anaerobic environments with low-temperature stress, such as high-latitude underground water, polar soil and even glacier sediment environments; meanwhile, the influence of extreme low temperature on the microbial fermentation iron reduction process is also known. The research is helpful for developing an innovative in-situ bioremediation technology so as to solve a series of ferric iron pollution problems in an alpine anaerobic environment.

The invention discloses a promoter, a promoter library, a gene expression cassette, a recombinant expression vector, a recombinant cell and a preparation method. The provided promoter comprises a feature fragment 1 and / or a feature fragment 2; the sequence of the feature fragment 1 comprises VBCGHCDW, and the sequence of the feature fragment 2 comprises SNNSWWBBWB. The promoter provided by the invention shows the promoter activity in rhodococcus in a stable phase specific mode, can express a target gene in a stable phase without additionally adding an inducer, and does not influence the growth of cells.

The present invention relates to a microorganism belonging to the genus Rhodococcus, the genus Xanthobacter, the genus Ancylobacter, the genus Shewanella, or the genus Aeromonas, and a method for producing the same, and more specifically, to a microorganism belonging to the genus Rhodococcus, the genus Xanthobacter, the genus Ancylobacter, the genus Shewanella, or the genus Aeromonas. The microorganism can be Rhodococcus hedyotidis (Rhodococcus cerevisiae) of the genus Rhodococcus, Xanthobacter flavus (Xanthobacter flavus) of the genus Xanthobacter, or Ancylobacter rudongensis of the genus Ancylobacter, and the microorganism can be a microorganism of the genus Ancylobacter, or a microorganism of the genus Ancylobacter, or a microorganism of the genus Ancylobacter, or a microorganism of the genus Ancylobacter and a microorganism of the genus Ancylobacter. The microorganism can be used for promoting the growth of blue algae, green algae, grey algae or euglena. The microorganism can be used to promote the growth of an organism belonging to the phylum cyanobacteria, unequal flagellum, euglenophyta, cryptophyta, rhodophyta, chlorophyta or chalaphyta.

The invention belongs to the field of biological breeding, and relates to rhodococcus and application and method thereof in toadstool cultivation. According to the rhodococcus sp. QD20240815 disclosed by the invention, the Latin is Rhodococcus sp. QD20240815, the collection date is September 15, 2025, the collection unit is the China Center for Type Culture Collection, and the collection number is CCTCC NO: M20252031. The rhodococcus QD20240815 disclosed by the invention is a beneficial bacterium of morchella, and is introduced into a morchella hypha cell to establish an internal symbiotic relationship between the rhodococcus QD20240815 and the morchella hypha cell, so that the growth capability of an internal symbiotic morchella strain can be improved, the stress resistance of the internal symbiotic morchella strain can be enhanced, and the yield of artificial cultivation of the internal symbiotic The technology provided by the invention provides a new microbial germplasm resource for application of a co-biochemical breeding technology in the morchella, is beneficial to high-yield and high-antibacterial strain breeding of the morchella, and promotes continuous and stable development of the morchella industry.

The invention discloses a rhodococcus strain with broad-spectrum plasticizer degradation capacity and application of the rhodococcus strain for coding alkyl sulfatase. The rhodococcus strain is preserved in China Center for Type Culture Collection on April 27, 2025 with the preservation name of SPR1 and the preservation number of CCTCC NO.M 2025907. The invention further discloses a preparation method of the rhodococcus strain. Gene sequence analysis and identification prove that the strain is rhodococcus fangensis of rhodococcus genus, is named as SPR1, and belongs to gram-positive bacteria. The SPR1 strain is capable of efficiently degrading a plurality of traditional plasticizers (such as dioctyl phthalate) and novel plasticizers (such as trioctyl trimellitate). In addition, the invention discloses the novel carboxyl esterase activity of alkyl sulfatase (named as TOTMAseA) encoded by the strain for the first time, and proves that the enzyme plays a key role in degradation of trioctyl trimellitate. The strain and enzyme gene resources thereof have important research and development values and wide engineering application prospects in the fields of plastic waste treatment and plasticizer polluted environment restoration.

The invention provides a strain of Rhodococcus sp. D-12 and an application thereof, and belongs to the technical field of microorganisms. The Rhodococcus sp. D-12 is preserved in the China Center for Type Culture Collection on November 20, 2025, the preservation number is CCTCC NO: M20252603, the Rhodococcus sp. D-12 can be applied to degradation of five kinds of phthalic acid esters at the same time, and the biological treatment effect of PAEs is improved.

The invention provides a construction method and application of a P450 fusion protein. The construction method comprises the following steps: step (1), fusing abamectin oxidation type P450 Ema1 with a Rhodococcus P450RhF-RPs reductase structural domain by adopting a connecting peptide to obtain Ema1-Rhf; and (2) carrying out point mutation on the Ema1-Rhf, and constructing the P450 fusion protein. According to the invention, redox partners derived from Rhodococcus are connected with a structural domain of P450 Ema1 through a designed connecting peptide; the cytochrome P450 monooxygenase mutant further improves the enzyme activity in the process of catalyzing abamectin to be converted into methylamino abamectin, the maximum conversion rate can be improved by 2.2 times through determination, and the mutant achieves 90% conversion rate within 39.8 hours, which is far better than the expression of an unfused system. And the method has certain significance on industrial production of the emamectin benzoate.

The invention provides a flora with PAEs degradation and siderophore secretion capabilities as well as a product and application thereof, and belongs to the technical field of soil remediation. The microbial community comprises Arthrobacter sp. Y-2, Rhodococcus sp. D-12, Rhodococcus sp. 2G, Bacillus mucilaginosus Krassica nikov 19749 and Pantoea sp. 60226, the Arthrobacter sp. Y-2 is preserved in the China Center for Type Culture Collection on November 20, 2025, the preservation number is CCTCC NO: M20252602, the Rhodococcus sp. D-12 is preserved in the China Center for Type Culture Collection on November 20, 2025, the preservation number is CCTCC NO: M20252603, and the Rhodococcus sp. D-12 can be applied to simultaneous degradation of six PAEs, can be applied to degradation of Pantoea sp. The composite degradation efficiency of the strain is obviously superior to that of any single strain, and meanwhile, the strain has the capability of secreting siderophores, so that the treatment efficiency of microorganisms on PAEs is enhanced.

The invention discloses rhodococcus sp. NJPE1, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 20, 2024, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 1.64773. The rhodococcus sp. The invention also provides a screening method of the rhodococcus and application of the strain in degradation of phthalate, polycyclic aromatic hydrocarbon, polychlorinated biphenyl and polybrominated diphenyl ether. The rhodococcus NJPE1 obtained through screening has a good degradation effect on PAEs of different structures, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, polybrominated diphenyl ethers and the like.

This invention relates to a method for enhancing the remediation of petroleum hydrocarbon-contaminated soil and synergistically improving soil carbon sequestration using indigenous degrading bacteria, belonging to the field of ecological environmental protection and remediation technology. Highly efficient petroleum-degrading bacteria strains Acinetobacter junii A2-1 and Rhodococcus sp. A17 were isolated and screened from petroleum hydrocarbon-contaminated soil and deposited at the China General Microbiological Culture Collection Center (CGMCC). The accession number for strain A2-1 is CGMCC No. 23724, and for strain A17 is CGMCC No. 23725. The A2-1 and A17 strains were prepared into a bio-enhancing agent, which was then inoculated into petroleum hydrocarbon-contaminated soil in appropriate amounts to improve the degradation efficiency of petroleum hydrocarbons. Furthermore, vegetation restoration synergistically enhances the soil's carbon sequestration function. This invention not only accelerates the remediation process of petroleum hydrocarbon-contaminated soil but also significantly improves the carbon sequestration capacity of petroleum hydrocarbon-contaminated soil, which is of great significance for the remediation of contaminated soil and the achievement of the "carbon neutrality" goal.

The invention relates to rhodococcus capable of stably and efficiently degrading estrogen and application of the rhodococcus. The rhodococcus C1 is preserved in the China General Microbiological Culture Collection Center on May 15, 2023, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.27337. Compared with the prior art, the Rhodococcus sp C1 provided by the invention can efficiently degrade the natural estrogen 17-beta estradiol, is tolerant to high-salt and heavy metal ions, can stably degrade the 17-beta estradiol, and has the application potential of repairing estrogen pollution.

The present invention belongs to the technical field of organic pollutant treatment, and specifically relates to a strain of Rhodococcus YZ-1 with high efficiency in pentane degradation. The Rhodococcus of the present invention is named Rhodococcus ( Rhodococcus sp.) YZ-1, which was deposited in China Center for Type Culture Collection on July 13, 2022, with a deposit number of CCTCC No: M 20221106. The Rhodococcus sp. of the present invention ( Rhodococcus Rhodococcus sp. YZ-1 can grow and reproduce using pentane as its sole carbon and energy source, adapts to a wide range of environmental temperatures and pH values, and can efficiently degrade pentane at initial concentrations up to 600 mg / L. Furthermore, the bacterium can also use n-hexane, cyclohexane, 2-methylpentane, and 3-methylpentane as carbon and energy sources, efficiently degrading n-hexane, 2-methylpentane, and 3-methylpentane. The Rhodococcus sp. YZ-1 provided by the present invention will play an important role in the practical treatment of pentane, n-hexane, cyclohexane, 2-methylpentane, and 3-methylpentane waste gas and wastewater.

The invention provides a composite functional flora and a method for simultaneously degrading six phthalic acid esters, and belongs to the technical field of soil remediation. The composite functional flora comprises Rhodococcus sp. D-12, Arthrobacter sp. Y-2 and Bacillus sp. W34, the Rhodococcus sp. D-12 is preserved in the China Center for Type Culture Collection on November 20, 2025 with the preservation number of CCTCC NO: M20252603, the Arthrobacter sp. Y-2 is preserved in the China Center for Type Culture Collection on November 20, 2025 with the preservation number of CCTCC NO: M20252602, and the Bacillus sp. W34 is preserved in the China Center for Type Culture Collection on November 20, 2025 with the preservation number of CCTCC NO: M20252602 with the preservation number of CCTCC NO: M20252603. The composite functional flora can be used for degrading six phthalic acid esters at the same time, the degradation effect is obviously higher than the independent degradation effect of any strain, and the biological treatment effect of PAEs is improved.