94 results about "Rhodococcus sp." patented technology

The invention relates to rhodococcus with the function of removal of ammonia nitrogen and nitrite in water and a low cost rhodococcus microbial agent production method and an application method, and belongs to the technical field of environmental microorganisms. The adopted strain is an efficient denitrification bacteria-(Rhodococcus sp.) YY12 strain separated and screened from activated sludge of sewage treatment plants, and the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No. 6924. According to the method, monosodium glutamate wastewater is used as a fermentation medium for large-scale culture of the Rhodococcus, the microbial agent for efficient removal of ammonia nitrogen and nitrite in water is prepared, and the process has the advantages of low production cost, simple operation, resource utilization of the monosodium glutamate wastewater, and reduction of pollution emissions. The microbial agent used for aquaculture not only can improve the water quality of aquaculture water, but also can significantly improve the aquatic products output, and shows a wide application prospect.

The invention discloses a rhodococcus sp. strain and application thereof, belonging to the field of environmental microbiological application. The strain is named as (Rhodococcus sp.) CM-HZX1 and is now collected with the serial number of CCTCC (China Center For Type Culture Collection) NO:M 2014329 in CCTCC on July 9th, 2014. The rhodococcus sp.CM-HZX1 has relatively strong phenol degrading capacity, namely that the degradation rate is up to 93.6% after 0.5g / L of phenol is degraded for 24 hours, and the degradation rate is up to more than 90% after 1.5g / L of phenol is degraded for 48 hours. The strain is capable of tolerating the salinity of 4% and strong in adaptability; and the strain is efficient when being used for biological treatment of phenol wastewater so as to be wide in application prospect.

The present invention relates to a novel Rhodococcus bacterium and to a process of hydrolyzing a cyano group of a nitrile compound using a novel Rhodococcus bacterium to produce the corresponding carboxylic acid. The present invention also relates to a process of producing carboxylic acids, in particular cyano carboxylic acids using a transformant transformed with a plasmid containing a nitrilase gene, a nitrile hydratase gene and an amidase gene derived from Rhodococcus bacteria capable of exhibiting particularly excellent position selectivity for the cyano group of aromatic polynitrile compounds, to such a transformant, such a plasmid, to such genes, to a process of producing an enzyme using the transformant, and to enzymes obtained by the process. The carboxylic acids, in particular cyano carboxylic acids obtained by the present invention are useful as starting materials for the synthesis of drugs, agrochemicals, dyestuffand other chemicals.

The invention relates to Rhodococcus sp., lipopeptid extra-cellular bio-demulsifier produced by culturing the Rhodococcus sp. and an application thereof. The dosage of the bio-demulsifier is 100-200mg / L, and the demulsification rate of the W / O type kerosene model emulsion can be 72.7-89.1% within 24h when the demulsification test temperature is 35 DEG C. When the bio-demulsifier is used in thin oil extraction liquid in ocean extraction plants of Shengli Oil Field (SLOF) and the dosage is 90-140mg / L, the demulsification rate can achieve 60.0-62.5% within 6.5h which is further superior to the chemical demulsifier used on site. The extra-cellular bio-demulsifier has the advantages of convenient extraction, low production cost, easy storage and transportation, and can solve the problems of influenced oil product quality and high extraction cost of cell wall combined products when full culture liquid is added.

An objective of the present invention is to provide a nitrile hydratase capable of producing 2-hydroxy-4-methylthiobutyroamide. The present invention provides a novel nitrile hydratase producing alpha-hydroxyamide using, alpha-hydroxnitrile as the substrate, and the encoding DNA thereof. The enzyme can be obtained from Rhodococcus sp. Further, the enzymatic activity of the enzyme can he maintained stably during the reaction. The present invention provides a method for producing amide compounds, the method comprising the step of reacting this enzyme to nitrile compounds. According to the present invention, from hydroxy nitrile compounds, corresponding amide compounds can be produced biochemically without reducing the enzyme activity of nitrile hydratase.

The invention discloses a bacterium capable of degrading herbicides and an application thereof. The bacterium capable of degrading herbicides is rhodococcus sp. 198-R-56, and the collection number of the bacterium in the China General Microbiological Culture Collection Centre is CGMCC No. 9868. With regard to the rhodococcus sp. 198-R-56 CGMCC No. 9868 provided by the invention, in an inorganic salt culture medium for 7 days, the degradation rate for 100mg / L of imazethapyr achieves 84.63%, the degradation rate for 50mg / L of acetochlor achieves 69.15%, the degradation rate for 100mg / L of chlorimuron-ethyl achieves 52.20%, and the degradation rate for 100mg / L of acifluorfen sodium achieves 20.16%, which indicates that the strain is capable of degrading the herbicides imazethapyr, acetochlor, chlorimuron-ethyl and acifluorfen sodium; meanwhile, the strain has a capacity of producing plant hormone heteroauxin and has a yield of 62.35+ / -3.76mu g / ml, which indicates that the strain has a capacity of promoting plant growth.

The invention discloses a 17 beta-estradiol degrading strain and an application thereof. A rhodococcus sp. JX-2 is preserved in China general microbiological culture collection center at September 4, 2014, and the strain preservation number is CGMCC NO.9636. An immobilized 17 beta-estradiol degrading bacterial agent produced by the rhodococcus sp. JX-2 is provided, a JX-2 bacterial suspension is mixed evenly with a sodium alginate solution, a CaCl2 solution is used as a crosslinking agent, crosslinking is carried out for 5.5-6.5 h, and immobilized small balls with a diameter of about 3-5 mm are obtained. The rhodococcus sp. JX-2 obtained from screening enables the 17 beta-estradiol degradation rate to reach 90% or more under laboratory shake flask culture conditions. The immobilized bacterial agent is applied directly in 17 beta-estradiol polluted water bodies and livestock and poultry manure, and can allow 17 beta-estradiol residues to be reduced by 60% or more in a short period of time.

The invention relates to a biochemical preparation for treating threonine fermentation sewage. The biochemical preparation is characterized by comprising a physical adsorbent and a composite microbial agent, wherein the composite microbial agent is prepared from the following raw materials by a volume ratio: 6 to 7 parts of arthrobacter sp., 5 to 7 parts of pseudomonas stutzeri, 5 to 7 parts of rhodococcus sp., 4 to 5 parts of bacillus amyloliquefaciens, 4 to 5 parts of clostridium papyrosolvens and 3 to 4 parts of scenedesmus obliquus. The biochemical preparation contains multiple microorganisms having good degrading capability to recalcitrant pollutants; various strains are reasonably matched to realize a good degrading effect; the biochemical preparation has a broad application prospect.

The invention discloses rhodococcus sp. and application of the rhodococcus sp. to zearalenone degradation. The preservation number of the rhodococcus sp. is CGMCC No.7186. The invention also provides a biological detoxication agent containing the preserved bacterial strain of the rhodococcus sp. and a preparing method of the biological detoxication agent. In addition, the invention also provides application of the bacterial strain or the biological detoxication agent to the zearalenone degradation.

The invention belongs to the technical field of pollutant biological repair, in particular relates to rhodococcussp WB-1, a separation and culture method of the rhodococcussp and a method for degrading polychlorinated biphenyl. A bacterial strain is a gram-positive bacterium and is verified to be the rhodococcussp, and a CGMCC preservation number is 5500. The bacterial strain can grow with biphenyl as the only carbon source and energy, can degrades more than 90% 2,3-dichloro diphenyl of 1mg / L, 2,4-dichloro diphenyl, 3,4-dichloro diphenyl and 2,4,4-trichlorine diphenyl in 24 hours, further can degrade more than 90% of 2,4,4-trichlorine diphenyl of 10mg / L and more than 50% of 2,2',3,3'-tetrachloro diphenyl in 72 hours. The application range of the bacterial strain comprises treatment of sewage containing the polychlorinated biphenyl and repair of polychlorinated biphenyl polluted soil.