9 results about "Rhodococcus strain" patented technology

The invention discloses a rhodococcus ruber WM-5 strain, and the preservation number of the rhodococcus ruber WM-5 strain is CCTCC (China Center for Type Culture Collection) NO.M2025874. Research shows that the rhodococcus ruber WM-5 strain has the functions of resisting cadmium and degrading phthalic acid ester, has extremely high degradation efficiency on DBP (dibutyl phthalate), and is relatively wide in suitable degradation condition range; the Rhodococcus ruber WM-5 strain has strong tolerance to Cd < 2 + > and PAEs, when the Rhodococcus ruber WM-5 strain is applied to PAEs polluted solution systems under the condition of different initial concentrations of PAEs and Cd-PAEs composite polluted solution systems with different initial concentrations of Cd < 2 + >, high PAEs degradation efficiency can be maintained, and technical support is provided for remediation of Cd-PAEs composite pollution. In a word, the invention makes up the technical blank of Cd-resistant PAEs degrading strains, and the Rhodococcus ruber WM-5 strain or the microbial agent thereof has great application value in the aspects of degrading phthalate and repairing Cd-PAEs composite pollutants.

The invention relates to the technical field of micro-ecology, in particular to a rhodococcus coryneform strain and application thereof in aquaculture. The invention discloses a strain of Rhodococcus corynobacteria, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.23799. The invention further discloses a preparation method of the strain of Rhodococcus corynobacteria. The rhodococcus strain can oxidize sulfides under the condition of low dissolved oxygen, does not reduce the pH value of a water body, reduces sulfide pollution of an aquaculture water body, relieves bottom mud acidification, and has no toxic effect on cultured animals.

The invention belongs to the technical field of biotechnology and biocatalysis, and particularly relates to rhodococcus etherivorans and application thereof. The rhodococcus etherivorans strain disclosed by the invention can be used for effectively degrading p-methoxybenzonitrile to generate p-methoxybenzoic acid, and the hydrolysis conversion rate is high. The rhodococcus etherivorans strain disclosed by the invention also shows hydrolytic activity to other nitrile compounds, and shows unique hydrolytic activity to nitrile compounds.

This invention discloses a compound microbial agent for processing wastewater from Trichosanthes kirilowii seeds and its preparation method, relating to the field of environmental microbiology. The compound microbial agent is composed of saponin-degrading strains, polysaccharide-degrading strains, and decolorizing strains in a volume ratio of 3:2:1. The saponin-degrading strains are Bacillus strains, the polysaccharide-degrading strains are Pseudomonas strains, and the decolorizing strains are Rhodococcus strains. The preparation method includes: isolating and screening functional strains from Trichosanthes kirilowii planting soil and wastewater from the processing workshop; expanding the culture to a bacterial concentration of CFU / mL or higher; mixing them in a volume ratio of 3:2:1; adding a protective agent; and drying to prepare a powder with a viable count greater than CFU / g. This compound microbial agent is added to the biochemical treatment unit at 0.01%-0.05% of the wastewater volume. Microbial community acclimatization can be completed within 7-14 days, effectively improving COD removal rate, saponin degradation rate, and color removal rate. It is suitable for the efficient biological treatment of Trichosanthes kirilowii seed processing wastewater.

The invention belongs to the technical field of estrogen degradation, and particularly discloses an application of low-temperature-resistant SDR dehydrogenase in estradiol degradation, and the low-temperature-resistant SDR dehydrogenase RQCSRS03565 is obtained by screening from Rhodococcus RCBS9, expressing in Escherichia coli and purifying. According to the invention, a novel SDR dehydrogenase RQCSRS03565 is identified and functionally verified from a rhodococcus strain RCBS9 for the first time, and the novel SDR dehydrogenase RQCSRS03565 has the characteristic of low temperature resistance and can still efficiently degrade E2 at a low temperature of 10 DEG C. Through structural simulation and site-directed mutagenesis experiments, the SER-205 is identified as a key functional residue of the enzyme for catalyzing estradiol degradation for the first time. Discovery of the low-temperature-resistant degrading enzyme and identification of key residues of the low-temperature-resistant degrading enzyme provide core elements and theoretical bases for development of efficient estradiol bioremediation technologies and preparations suitable for low-temperature environments, and the low-temperature-resistant degrading enzyme has important application value.

The invention relates to an agricultural pest biological control technology, and aims to provide a rhodococcus strain and application thereof to degradation of insecticides and enhancement of parasitic wasp resistance. The strain is preserved in the China Center for Type Culture Collection, the preservation name is Fanqing Sheng Rhodococcus IT-1, the classification name of Latin is Rhodococcus qingshengii, and the preservation number is CCTCC NO: M 20252461. The 16S rRNA gene sequence of the strain is as shown in SEQ ID NO: 1. The strain is unique in source and broad in function spectrum; biological control can be enhanced, and the effect is remarkable; the strain is supplemented back into the parasitic wasp body in a feeding mode, the endurance capacity of the parasitic wasp to imidacloprid and thiamethoxam can be remarkably improved, and the strain has great significance in maintaining and enhancing the biological control function of an agricultural ecosystem and reducing dependence of chemical pesticides.

The present application relates to the field of microbial technology, and particularly to a Rhodococcus Z13 strain and application thereof.The screened Rhodococcus Z13 strain is identified as a new species of Rhodococcus genus, and is named as Rhodococcus ferula, with a preservation number of GDMCC No.62880; and the strain has the characteristics of high-temperature resistance, and can normally grow at a maximum temperature of 45 DEG C.Through experiments, it is proved that under the high-temperature condition of 45 DEG C, the Rhodococcus Z13 can completely degrade phenol with a concentration of 1300 mg / L within 30 h; it can be seen that the screened strain has high efficiency in the biological treatment of phenolic wastewater, and has wide application prospect.

The invention discloses a rhodococcus strain with broad-spectrum plasticizer degradation capacity and application of the rhodococcus strain for coding alkyl sulfatase. The rhodococcus strain is preserved in China Center for Type Culture Collection on April 27, 2025 with the preservation name of SPR1 and the preservation number of CCTCC NO.M 2025907. The invention further discloses a preparation method of the rhodococcus strain. Gene sequence analysis and identification prove that the strain is rhodococcus fangensis of rhodococcus genus, is named as SPR1, and belongs to gram-positive bacteria. The SPR1 strain is capable of efficiently degrading a plurality of traditional plasticizers (such as dioctyl phthalate) and novel plasticizers (such as trioctyl trimellitate). In addition, the invention discloses the novel carboxyl esterase activity of alkyl sulfatase (named as TOTMAseA) encoded by the strain for the first time, and proves that the enzyme plays a key role in degradation of trioctyl trimellitate. The strain and enzyme gene resources thereof have important research and development values and wide engineering application prospects in the fields of plastic waste treatment and plasticizer polluted environment restoration.

This invention relates to a method for enhancing the remediation of petroleum hydrocarbon-contaminated soil and synergistically improving soil carbon sequestration using indigenous degrading bacteria, belonging to the field of ecological environmental protection and remediation technology. Highly efficient petroleum-degrading bacteria strains Acinetobacter junii A2-1 and Rhodococcus sp. A17 were isolated and screened from petroleum hydrocarbon-contaminated soil and deposited at the China General Microbiological Culture Collection Center (CGMCC). The accession number for strain A2-1 is CGMCC No. 23724, and for strain A17 is CGMCC No. 23725. The A2-1 and A17 strains were prepared into a bio-enhancing agent, which was then inoculated into petroleum hydrocarbon-contaminated soil in appropriate amounts to improve the degradation efficiency of petroleum hydrocarbons. Furthermore, vegetation restoration synergistically enhances the soil's carbon sequestration function. This invention not only accelerates the remediation process of petroleum hydrocarbon-contaminated soil but also significantly improves the carbon sequestration capacity of petroleum hydrocarbon-contaminated soil, which is of great significance for the remediation of contaminated soil and the achievement of the "carbon neutrality" goal.