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A kind of anti-high temperature floc producing strain and its cultivation and application method

A high-temperature, strain-resistant technology, applied in the field of microbiology, can solve the problems of large amount of flocculant, low flocculation rate, complicated operation, etc., and achieve the effect of low amount of use, simple cultivation and operation process, and high flocculation activity

Inactive Publication Date: 2016-03-09
NORTHEASTERN UNIV LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of them are bacterial strains such as Paenibacillus polymyxa, Paenibacillus terrae and their microbial preparations applied to the prevention and control of plant diseases and insect pests in the agricultural field; Paenibacillus polymyxa and Paenibacillus colloid are used to produce microbial fertilizers to achieve Recycling of sludge, or for the preparation of exopolysaccharides to obtain exopolysaccharides and live microbial preparations, etc., but there are few studies on the application of Paenibacillus as a flocculant in water treatment
At the same time, in the fermentation and purification process of microbial flocculant, there are also problems such as complicated operation, large amount of flocculant, and low flocculation rate.

Method used

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  • A kind of anti-high temperature floc producing strain and its cultivation and application method
  • A kind of anti-high temperature floc producing strain and its cultivation and application method
  • A kind of anti-high temperature floc producing strain and its cultivation and application method

Examples

Experimental program
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Effect test

Embodiment 1

[0061] The method for producing microbial fermented liquid by using Paenibacillus resistant to high temperature and using it as a flocculant for kaolin suspension, the specific steps are as follows:

[0062] (1) Strain activation: Pick a ring of Paenibacillus sp. CGMCC No.2040 bacterial lawn from the slant medium and inoculate it in the liquid medium, and incubate at 30°C for 24 hours on a shaking table with a rotation speed of 140r / min. The activation can be completed;

[0063] (2) Fermentation culture: inoculate the activated bacterial liquid in the optimized medium at a volume ratio of 2% to continue culturing for 48 hours to obtain a microbial flocculant fermentation liquid.

[0064] (3) Kaolin flocculation: Add 0.5g of kaolin to a 100mL graduated cylinder, add 100mL of water and shake well to prepare a suspension of kaolin, add 0.05mL of fermentation broth, shake well and let stand for 5min, take 50mL of supernatant and use spectrophotometry Measuring the optical density...

Embodiment 2

[0066] The method for cultivating and producing microbial fermentation liquid and using it as a flocculant for kaolin suspension after high-temperature heat treatment by using heat-resistant Paenibacillus bacillus, the specific steps are as follows:

[0067] (1) Strain activation: Pick a ring of Paenibacillus sp. CGMCC No.2040 bacterial lawn from the slant culture medium and inoculate it in the liquid medium, and culture it on a shaking table with a rotation speed of 160r / min at 28°C for 24h The activation can be completed;

[0068] (2) High-temperature heating of the strain: heat the activated bacterial liquid at 80° C. for 20 minutes.

[0069] (3) Fermentation culture: Inoculate the heated bacterial solution in the optimized medium at a volume ratio of 0.1% and continue to cultivate for 72 hours to obtain a microbial flocculant fermentation solution.

[0070] (4) Kaolin flocculation: Add 0.5g of kaolin to a 100mL graduated cylinder, add 100mL of water and shake well to prep...

Embodiment 3

[0074] The method for cultivating and producing microbial fermentation liquid and using it as a flocculant for kaolin suspension after high-temperature heat treatment by using heat-resistant Paenibacillus bacillus, the specific steps are as follows:

[0075] (1) Strain activation: Pick a ring of Paenibacillus sp. CGMCC No. 2040 lawn from the slant culture medium and inoculate it in the liquid medium, and culture it on a shaking table with a rotation speed of 140r / min at 30°C for 24h The activation can be completed;

[0076] (2) Strain heating at high temperature: heat the activated bacterial liquid at 60°C for 30 minutes;

[0077] (3) Fermentation culture: Inoculate the heated bacterial solution in the optimized medium at a volume ratio of 1% and continue to cultivate for 60 hours to obtain a microbial flocculant fermentation solution.

[0078] (4) Kaolin flocculation: Add 0.5g of kaolin to a 100mL graduated cylinder, add 100mL of water and shake well to prepare a suspension ...

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Abstract

A high-temperature resistant floc-producing strain and its cultivation and application methods belong to the field of microbiology. Preserved by the General Microbiology Center (CGMCC) of the China Council for the Collection of Microbial Cultures, preservation number: CGMCC? No.2040, classification name: Paenibacillus? sp. Date of preservation: May 11, 2007. The high-temperature-resistant Paenibacillus strain screened from fruit tree soil is the first new strain discovered in China. After being heated at 40 to 80°C for 5 to 60 minutes, it is cultured through aerobic fermentation at 25 to 35°C. The fermentation liquid is used as a flocculant for kaolin suspensions. When the ratio is 0.01% to 0.2% (v / v), the flocculation rate is 88% to 97%. When the Paenibacillus and its fermentation liquid provided by the invention are used as kaolin suspension flocculant, the cultivation operation process is simple, the cost is low, the flocculation activity is high, the usage is low, and there is no secondary pollution.

Description

technical field [0001] The invention belongs to the field of microbiology, and particularly relates to a high-temperature-resistant flocculation-producing strain and a cultivation and application method thereof. Background technique [0002] Flocculant, also known as settling agent, is a kind of substance that can coagulate and precipitate solid suspended particles that are not easy to settle in liquid. Flocculation technology is widely used in water supply, wastewater treatment and sludge dehydration. It is an effective way to improve water treatment efficiency. An economical and simple treatment method, the flocculation effect often determines the operation status of the subsequent process, the final effluent water quality and cost. In the process of flocculation treatment, the type, nature and variety of flocculant directly affect the treatment effect, so flocculant is the core of water treatment by flocculation method. [0003] The currently used flocculants can be divi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/52C12R1/01
Inventor 胡筱敏姜彬慧邓述波付丽丽赵鑫杨程程李凤达刘金亮
Owner NORTHEASTERN UNIV LIAONING
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