A kind of anti-high temperature floc producing strain and its cultivation and application method
A high-temperature, strain-resistant technology, applied in the field of microbiology, can solve the problems of large amount of flocculant, low flocculation rate, complicated operation, etc., and achieve the effect of low amount of use, simple cultivation and operation process, and high flocculation activity
Image
Examples
Embodiment 1
[0061] The method for producing microbial fermented liquid by using Paenibacillus resistant to high temperature and using it as a flocculant for kaolin suspension, the specific steps are as follows:
[0062] (1) Strain activation: Pick a ring of Paenibacillus sp. CGMCC No.2040 bacterial lawn from the slant medium and inoculate it in the liquid medium, and incubate at 30°C for 24 hours on a shaking table with a rotation speed of 140r / min. The activation can be completed;
[0063] (2) Fermentation culture: inoculate the activated bacterial liquid in the optimized medium at a volume ratio of 2% to continue culturing for 48 hours to obtain a microbial flocculant fermentation liquid.
[0064] (3) Kaolin flocculation: Add 0.5g of kaolin to a 100mL graduated cylinder, add 100mL of water and shake well to prepare a suspension of kaolin, add 0.05mL of fermentation broth, shake well and let stand for 5min, take 50mL of supernatant and use spectrophotometry Measuring the optical density...
Embodiment 2
[0066] The method for cultivating and producing microbial fermentation liquid and using it as a flocculant for kaolin suspension after high-temperature heat treatment by using heat-resistant Paenibacillus bacillus, the specific steps are as follows:
[0067] (1) Strain activation: Pick a ring of Paenibacillus sp. CGMCC No.2040 bacterial lawn from the slant culture medium and inoculate it in the liquid medium, and culture it on a shaking table with a rotation speed of 160r / min at 28°C for 24h The activation can be completed;
[0068] (2) High-temperature heating of the strain: heat the activated bacterial liquid at 80° C. for 20 minutes.
[0069] (3) Fermentation culture: Inoculate the heated bacterial solution in the optimized medium at a volume ratio of 0.1% and continue to cultivate for 72 hours to obtain a microbial flocculant fermentation solution.
[0070] (4) Kaolin flocculation: Add 0.5g of kaolin to a 100mL graduated cylinder, add 100mL of water and shake well to prep...
Embodiment 3
[0074] The method for cultivating and producing microbial fermentation liquid and using it as a flocculant for kaolin suspension after high-temperature heat treatment by using heat-resistant Paenibacillus bacillus, the specific steps are as follows:
[0075] (1) Strain activation: Pick a ring of Paenibacillus sp. CGMCC No. 2040 lawn from the slant culture medium and inoculate it in the liquid medium, and culture it on a shaking table with a rotation speed of 140r / min at 30°C for 24h The activation can be completed;
[0076] (2) Strain heating at high temperature: heat the activated bacterial liquid at 60°C for 30 minutes;
[0077] (3) Fermentation culture: Inoculate the heated bacterial solution in the optimized medium at a volume ratio of 1% and continue to cultivate for 60 hours to obtain a microbial flocculant fermentation solution.
[0078] (4) Kaolin flocculation: Add 0.5g of kaolin to a 100mL graduated cylinder, add 100mL of water and shake well to prepare a suspension ...
PUM
Login to View More Abstract
Description
Claims
Application Information
- IPC
- C12N1/20; C02F3/34; C02F1/52; C12R1/01
- Inventors
- 胡筱敏; 姜彬慧