Method for preparing biological flocculent by biological hydrogen-making waste liquid
A biological flocculant, biological hydrogen production technology, applied in the direction of flocculation/sedimentation water/sewage treatment, etc., can solve the problems of low yield of biological flocculant, high production cost, and not reaching industrial application.
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specific Embodiment approach 1
[0004] Specific embodiment 1: The method for producing biological flocculant by using biological hydrogen production waste liquid in this embodiment is carried out according to the following steps: (1) Mix hydrogen production waste liquid and flocculant producing bacteria culture medium, and prepare a culture medium (2) transfer the domesticated strains to the waste liquid of biological hydrogen production, under the conditions of temperature 30°C and shaker speed 140r / min When the flocculation rate is greater than 50%, the acclimatization is over, and the bioflocculant is obtained. The biological hydrogen production waste liquid comes from the ethanol-type fermentation biological hydrogen production reactor of the Environmental Biotechnology Research Center of the Municipal Environmental Engineering College of Harbin Institute of Technology. The COD concentration is greater than 4000mg / L, and the pH value is 4.0-4.5. The biological hydrogen production waste liquid is composed...
specific Embodiment approach 2
[0005] Specific implementation mode two: this implementation mode is realized in this way:
[0006] 1. Compound flocculogen F 2 -F 6 domestication of:
[0007] a. Set up 9 groups of 250mL culture bottles, take a total of 150mL of the mixture of hydrogen production waste liquid and flocculant producing bacteria culture medium, put it into the culture bottle for sterilization, and the ratio of culture medium and hydrogen production waste liquid in the culture bottle is 9:1 respectively , 6.5:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:6.5 and 1:9, in order to make the acclimatization medium similar to the flocculation medium, keep the culture medium Contains a mass percentage of 0.2% K 2 HPO 4 and 0.08% KH 2 PO 4 , wherein the biological hydrogen production waste liquid is the effluent of the fermentative biological hydrogen production reactor of the Environmental Biotechnology Research Center of Harbin Institute of Technology, its COD is about 4000mg / L, and its pH value is 4.0-4.5. De...
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