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Method for preparing biological sludge amendment and application thereof

A kind of sludge conditioning and bio-based technology, which is applied to the direction of fungus and fungi fixed on or in the inorganic carrier, which can solve the problem of high price of artificially synthesized organic polymer flocculants, difficult biodegradation of residues, and the amount of sludge conditioner Large and other problems, to achieve the effect of biodegradability and environmental friendliness, no secondary pollution, and good conditioning effect

Inactive Publication Date: 2008-03-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that commonly used sludge conditioners are large in dosage, have certain corrosiveness and toxicity, and cause secondary pollution to the environment. In order to solve the problems of biodegradation and threat to ecological security, it provides a high-efficiency, energy-saving and environmentally friendly preparation method of sludge conditioner and its application in sludge dewatering

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of biological sludge conditioner is prepared according to the following steps with Aspergillus sojae as raw material:

[0022] (1) Inoculate fresh mold spores into the fermentation medium, ferment in a bioreactor, the stirrer speed is 200rpm, the fermentation temperature is 30°C, and the fermentation time is 30h, collect the culture medium, filter and remove the bacteria with gauze to obtain the fermentation liquid ; Wherein the concentration of each component in the mold fermentation medium is glucose: 1%, NaCl: 0.1%, K 2 HPO 4 : 0.06%, KCl: 0.05%, MgSO 4 : 0.05%, FeSO 4 : 0.001%.

[0023] (2) Place the fermentation broth in step (1) in a 4°C refrigerator to pre-cool, then use 2 times the volume of absolute ethanol pre-cooled in a 4°C refrigerator to precipitate and extract, place it in the refrigerator for 16 hours, and discard the supernatant liquid, washed the precipitate with 75% ethanol, and vacuum freeze-dried the precipitate to obtain a crude product;...

Embodiment 2

[0028] A kind of biological sludge conditioner is prepared according to the following steps with Aspergillus sojae as raw material:

[0029] (1) Inoculate fresh mold spores into the fermentation medium, ferment in the bioreactor, the stirrer speed is 400rpm, the fermentation temperature is 33°C, the fermentation time is 32h, collect the culture medium, filter the bacteria with gauze, and obtain the fermentation liquid ; Wherein the concentration of each component in the mold fermentation medium is glucose: 2%, NaCl: 0.3%, K 2 HPO 4 : 0.10%, KCl: 0.10%, MgSO 4 : 0.10%, FeSO 4 : 0.001%.

[0030] (2) Pre-cool the fermentation broth in step (1) in a 4°C refrigerator, then use 2 times the volume of acetone pre-cooled in a 4°C refrigerator to precipitate and extract, place it in the refrigerator for 20 hours, and discard the supernatant , wash the precipitate with 75% ethanol, and vacuum freeze-dry the precipitate to obtain the crude product; the crude product is dissolved in di...

Embodiment 3

[0035] A kind of biological sludge conditioner is prepared according to the following steps with Aspergillus sojae as raw material:

[0036] (1) Inoculate fresh mold spores into the fermentation medium, ferment in the bioreactor, the stirrer speed is 300rpm, the fermentation temperature is 35°C, and the fermentation time is 35h, collect the culture medium, filter and remove the bacteria with gauze to obtain the fermentation liquid ; Wherein the concentration of each component in the mold fermentation medium is glucose: 1.5%, NaCl: 0.2%, K 2 HPO 4 : 0.08%, KCl: 0.07%, MgSO 4 : 0.08%, FeSO 4 : 0.001%.

[0037] (2) Place the fermentation broth in step (1) in a 4°C refrigerator to pre-cool, then use 2 times the volume of absolute ethanol pre-cooled in a 4°C refrigerator to precipitate and extract, place it in the refrigerator for 24 hours, and discard the above The supernatant was washed with 75% ethanol, and the precipitate was vacuum freeze-dried to obtain a crude product; t...

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Abstract

The present invention in particular relates to a preparation method of biological type sludge conditioning agent and the application thereof. The biological type sludge conditioning agent is prepared by using aspergillus sojae (Aspergillus sojae) as raw material, and the obtained sludge conditioning agent can be applied to the sludge dewatering. The coagulation activity of the conditioning agent of the present invention is high, the cost is low, the dosage output is high, the stability is strong, the applicable range is wide, and the present invention has the biological degradability and the environmental friendliness, and the second pollution is not caused.

Description

technical field [0001] The invention belongs to the technical field of sludge dewatering, in particular to a preparation method and application of a biological sludge conditioner. Background technique [0002] The water contained in the concentrated sludge of the sewage treatment plant can be divided into four categories: the interstitial water between the particles, which accounts for about 70% of the total water; the water in the capillary between the particles is about 20%; Water, about 10% of the total moisture. The purpose of adding the sludge conditioner is to remove the interstitial water of the particles through electrostatic adsorption, electrical neutralization, bridge network capture, etc., so that the fine and loose sludge particles aggregate into denser particles and settle down. [0003] At present, the commonly used sludge conditioners mainly include inorganic conditioners, organic polymer conditioners and natural polymer conditioners. Inorganic polymer cond...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N1/14
Inventor 尹华叶锦韶何宝燕张娜彭辉
Owner JINAN UNIVERSITY
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