Preparation method for biological flocculant
A biological flocculant and seed technology, applied in the direction of flocculation/sedimentation water/sewage treatment, fungi, etc., can solve the problems of narrow range of action, high cost, low flocculation effect, etc., and achieve non-toxic effects on the human body, simple production conditions, Good flocculation effect
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example 1
[0014] Prepare shaker medium by weight percentage, its composition is cornstarch 4%, urea 0.04%, ammonium sulfate 0.5%, sodium chloride 0.06%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.3%, add tap water to 3L , formulated as a liquid fermentation medium. Pack the culture medium in 1L shake flasks, each bottle contains 300ml of culture medium, inoculate the seed liquid of Aspergillus sojae according to 3% of the weight of the culture liquid, and cultivate it for 50 hours at a temperature of 29°C, pH 6, and ventilation conditions, and the number of rotations of the shaker is The temperature is 140rpm. Observe that the liquid in the shake flask is clear and the solid matter is uniform, then the culture is completed.
[0015] Filtrate and concentrate the fermented liquid (concentrate with thin film: flow rate 100L / h, temperature 60°C) to 1L, stir and add 95% ethanol to the concentrated liquid to make the final concentration of ethanol 66%, leave it at r...
example 2
[0022] Prepare 20L fermented liquid by weight percentage, its composition is: corn starch 5%, urea 0.05%, ammonium sulfate 0.6%, sodium chloride 0.08%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.4%, add water to required volume. Then inoculate the Aspergillus sojae seed solution according to 5% of the volume of the fermentation culture solution, and cultivate it with aeration at a temperature of 30° C., pH 7, and stirring for 58 hours.
[0023] Filtrate and concentrate the fermented liquid (concentrated by ultrafiltration) to about 5L, stir and add absolute ethanol to the concentrated liquid to make the final concentration of ethanol 63%, let it stand at room temperature for more than half an hour, centrifuge, and wash the precipitate with ethanol Washed twice and dried in vacuum to obtain 468g of crude flocculant.
[0024] Flocculation treatment of Linhe domestic sewage in Lintong District, Xi'an: according to the comparison of adding 0.015% floccu...
example 3
[0026] Prepare 23L fermentation broth by weight percentage, and its composition is: cornstarch 6%, urea 0.06%, ammonium sulfate 0.6%, sodium chloride 0.1%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.4%, add water to reach volume required. Then inoculate the Aspergillus sojae seed liquid according to 5% of the volume of the fermentation liquid, and cultivate it at a temperature of 30° C., pH 7, stirring and ventilation for 55 hours.
[0027] Filtrate and concentrate the fermented liquid (concentrated by thin film) to about 4.6L, stir and add absolute ethanol to the concentrated liquid to make the final concentration of ethanol 60%, let it stand at room temperature for more than half an hour, centrifuge and separate the precipitate with ethanol After washing once and vacuum drying, 613 g of crude flocculant was obtained.
[0028] The flocculation treatment of Xi'an Hanxing Paper Co., Ltd.'s papermaking water interruption: according to the control of...
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