Preparation method for biological flocculant

A biological flocculant and seed technology, applied in the direction of flocculation/sedimentation water/sewage treatment, fungi, etc., can solve the problems of narrow range of action, high cost, low flocculation effect, etc., and achieve non-toxic effects on the human body, simple production conditions, Good flocculation effect

Inactive Publication Date: 2008-02-13
ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After searching, the relevant patents in this field include: 1. the method for preparing bioflocculant by Arthrobacter (application number is 200310105515.X), from the perspective of the medium composition of its production, the cost is relatively high, and the pH of wastewater treatment is relatively high. In 6-9, the scope of action is relatively narrow, and its flocculation and sedimentation effect is not clear
2. A sphingella and a method for preparing a glycoprotein bioflocculant by using it (the application number is 200410023988.X), the flocculation effect of this flocculant is relatively low, and the cost is high; 3. It is prepared from Nocardia A biological flocculant (application number 01108045.0), whose flocculation effect is relatively low; ④The production bacteria and production process of microbial flocculants using bean dregs (application number 200610031485.6), the invention is prepared by Paenibacillus polymyxa Biological flocculant, its production process is relatively complicated, the flocculation rate of kaolin can reach 94%, and the flocculation effect is relatively low; In the flocculation effect test, the inorganic flocculant aluminum chloride was used as a coagulation aid, and aluminum chloride itself is a flocculant with a good flocculation effect. Therefore, the flocculation effect of the flocculant of the invention was not reflected.
[0004] In the above patent applications, there is generally a shortcoming that the flocculation effect of the prepared flocculant is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0014] Prepare shaker medium by weight percentage, its composition is cornstarch 4%, urea 0.04%, ammonium sulfate 0.5%, sodium chloride 0.06%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.3%, add tap water to 3L , formulated as a liquid fermentation medium. Pack the culture medium in 1L shake flasks, each bottle contains 300ml of culture medium, inoculate the seed liquid of Aspergillus sojae according to 3% of the weight of the culture liquid, and cultivate it for 50 hours at a temperature of 29°C, pH 6, and ventilation conditions, and the number of rotations of the shaker is The temperature is 140rpm. Observe that the liquid in the shake flask is clear and the solid matter is uniform, then the culture is completed.

[0015] Filtrate and concentrate the fermented liquid (concentrate with thin film: flow rate 100L / h, temperature 60°C) to 1L, stir and add 95% ethanol to the concentrated liquid to make the final concentration of ethanol 66%, leave it at r...

example 2

[0022] Prepare 20L fermented liquid by weight percentage, its composition is: corn starch 5%, urea 0.05%, ammonium sulfate 0.6%, sodium chloride 0.08%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.4%, add water to required volume. Then inoculate the Aspergillus sojae seed solution according to 5% of the volume of the fermentation culture solution, and cultivate it with aeration at a temperature of 30° C., pH 7, and stirring for 58 hours.

[0023] Filtrate and concentrate the fermented liquid (concentrated by ultrafiltration) to about 5L, stir and add absolute ethanol to the concentrated liquid to make the final concentration of ethanol 63%, let it stand at room temperature for more than half an hour, centrifuge, and wash the precipitate with ethanol Washed twice and dried in vacuum to obtain 468g of crude flocculant.

[0024] Flocculation treatment of Linhe domestic sewage in Lintong District, Xi'an: according to the comparison of adding 0.015% floccu...

example 3

[0026] Prepare 23L fermentation broth by weight percentage, and its composition is: cornstarch 6%, urea 0.06%, ammonium sulfate 0.6%, sodium chloride 0.1%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.4%, add water to reach volume required. Then inoculate the Aspergillus sojae seed liquid according to 5% of the volume of the fermentation liquid, and cultivate it at a temperature of 30° C., pH 7, stirring and ventilation for 55 hours.

[0027] Filtrate and concentrate the fermented liquid (concentrated by thin film) to about 4.6L, ​​stir and add absolute ethanol to the concentrated liquid to make the final concentration of ethanol 60%, let it stand at room temperature for more than half an hour, centrifuge and separate the precipitate with ethanol After washing once and vacuum drying, 613 g of crude flocculant was obtained.

[0028] The flocculation treatment of Xi'an Hanxing Paper Co., Ltd.'s papermaking water interruption: according to the control of...

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Abstract

The invention discloses a method to prepare bio-flocculant. The invention is characterized in that aspergillus fermentating cultivation is adopted. The aspergillus is agitated and fermented (28-30 DEG C, pH6-7, under ventilating condition) for 48-60 hours; the fermentation broth is filtered and condensed, and then proper amount of ethanol is added into the condensed broth and centrifugal separation is conducted after stewing; the deposition is washed with ethanol and dried in vacuum to achieve a crude product of fawn flocculant. The prevention method has the advantages of simple condition of production and low cost. The bio-flocculant prepared by the method is applicable to disposing sewage containing granular contaminants or filth, and the dosage generally drops in 0.1 to 0.3 per mill. It is proved by flocculation treatment that, compared with the prior art, the invention has sound flocculation effect, low flocculant speed and small dosage; besides, the invention is environment-friendly and harmless to human body.

Description

technical field [0001] The invention belongs to a method for preparing a biological flocculant by using microorganisms, and relates to the technical field of sewage treatment. Background technique [0002] The flocculation and sedimentation method occupies an extremely important position in water treatment. The proportion of domestic and foreign industrial wastewater treatment using the flocculation and sedimentation method accounts for 60%-75%, while almost 100% of the tap water industry uses the flocculation method as a means of water purification. At present, the flocculants widely used in water treatment mainly include inorganic polymer flocculants and organic polymer flocculants. Because inorganic flocculants are generally used in large amounts and are likely to cause secondary pollution to the environment, such as aluminum ions in the series of polyaluminium, they will cause harm to human health; while the residues of organic polymer flocculants (such as: polyacrylamid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F1/52
Inventor 岳淑宁马齐张强秦涛张红艳阮祥稳李世强李忠玲
Owner ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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