Method for preparing microbial flocculant by protoplast fusion technology

Abstract

The invention provides a method for preparing a microbial flocculant by a protoplast fusion technology, and belongs to the field of flocculant preparation. The method is characterized by (1) protoplast producing bacterium LV-1 regeneration conditions and preparation steps, specifically comprising carrying out an activation operation in a liquid complete medium (CM) for 5 to 10 hours based on an inoculation amount of 0.5 to 2%, adding 2 to 6 mol / L of glycine into the liquid complete medium before enzymolysis to carry out a treatment for 5 to 15 hours, carrying out an enzymolysis process in a water bath for 1 to 4 hours at a temperature of 37 DEG C and an enzyme concentration of 0.05 to 0.2 mg / mL, and coating a regeneration template containing cane sugar as a stabilizer, and (2) protoplast fusion strain LV-2 fermentation and flocculation conditions, specifically comprising a cane sugar content of 8 to 12 g / L, a lactose content of 5 to 10 g / L, an ammonium nitrate content of 0.4 to 1.2 g / L, a peptone content of 0.4 to 1.2 g / t, an extract yeast content of 0.1 to 0.4 g / L, a culture temperature of 20 to 40 DEG C and an optimal adding amount of 0.2 to 2%. Compared with the prior art, the method has the advantages of high effectiveness, simple process, board application scope, low cost and good biological safety.

Description

technical field [0001] The invention relates to a microbial flocculant, in particular to a method for preparing the microbial flocculant by utilizing protoplast fusion technology. Background technique [0002] Water is the most basic need for human survival. However, with the rapid development of industry, the problem of water pollution has become increasingly prominent. At the same time, the continuous improvement of people's material living standards has also put forward higher requirements for water quality. In the history of the development of water treatment agent flocculation technology, inorganic salt flocculants (such as aluminum sulfate, aluminum chloride, ferric chloride, etc.) were developed in the 1960s, but the treatment effect was not ideal. Agents (such as polyaluminum chloride, polyferric sulfate, etc.) have received good results in water treatment, but the aluminum ions of polyaluminum chloride will cause secondary pollution to the environment during use, an...

AI Technical Summary

Problems solved by technology

[0005] (1). High preparation cost: Most of the microbial flocculants are prepared according to the ideas of food fermentation and biopharmaceuticals, that is, a single strain and expensive medium are used, and the preparation cost is high

[0006] (2). The index for measuring the activity of flocculants is single: At present, the ability of microbial flocculants to treat kaolin suspensions is used to characterize their activity at home and abroad. For industrial wastewater with widely different properties and types, this index is obviously not comprehensive.

[0007] (3). Poor pertinence: When studying microbial flocculant wastewater treatment, there is no research on the corresponding relationship between flocculant types, components and wastewater treatment types, which will cause blindness and lack of microbial flocculant in the use process Targeted, making it difficult to improve the efficiency of microbial flocculants in wastewater treatment